SSGCID: Target Page

BaboA.17087.a

delta-aminolevulinic acid dehydratase

CENTER ID:	BaboA.17087.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.17087.a.A2.GE34105	mature protein		18	495

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_II001120
RefSeq:	XP_001609639.1
UniProt:	A7AT10

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLFWAISAL ALGINIPAGF YNESRFGIGV TYTDAVAIRR NDVQRISQAS RSSDSAAFVC VPSSQWAQPS TGVLDTQRGC ATSKVLAKRK EEGNLDSTPE GVEFDSDVDY EDIYDASDYE SDDVEGEDGS SSEYDSDEDT GNSAFDYNAR GELMLPNLTR LSRTRVSKNR HKLFRGRVLH LADLVYPLVV TDKESSSSIK EVPGLRLLSI PDLVKEVEEA RNLGITAFMI HPHIDKSLKS EFGDEGLNPD GLLPRAIDAV KEAFPDVQVF ADATVAHFSL DGHDGLVEPE TDVISNDVSV SQLAKQVTTL ASAGCDVVSL NDTLDGSVGV ARDALDFEGY TDVSLMSRAG KFNSVYMAQH QAMLGTTPAQ NVRVESYLHD VAIPDEPILK GVQDVDDGAD MVAVEPATAF VDVIRSLKDR LRSPVVAVHT TGEYRALKAA AAAGVYDEKA AAIEALRGLR RAGADLIVSS FAKDVAQWLL EDMQTNGMEN VALGV

NT Sequence

atgggactgt tttgggccat tagtgctctg gcccttggta tcaatattcc cgcaggattc tacaatgaat cgaggtttgg tatcggtgta acgtatactg atgccgttgc tatacgtcga aacgatgtgc aacgtatttc acaagcctca agatcgtcag acagcgcggc tttcgtttgt gttccatcga gccaatgggc gcaaccatca actggtgtgc tagacactca acggggatgt gctactagca aggtgctcgc caaacgtaaa gaagagggaa atttggattc cactcccgag ggtgtagaat ttgattctga tgtggactac gaggacattt atgacgctag tgactatgaa tctgatgacg tcgagggtga agacggatcc tcttctgagt atgattctga tgaagatacc ggtaatagtg cgtttgatta caatgcgcgt ggagagctta tgcttccaaa cctaacccgg ttatcgagaa cgcgagtgtc gaaaaaccgg cacaagctat tccgcggtcg cgttctccac ttggctgatt tggtatatcc tttagtagtc actgacaagg aatcttcatc gtctatcaag gaggtacctg gattgcggct attatcgatt cctgaccttg taaaggaggt tgaagaggca cgtaaccttg gaatcacagc tttcatgata cacccacaca tagacaagtc actaaagagc gagtttggtg acgagggttt gaaccctgat ggacttcttc caagggctat cgatgctgta aaagaggcgt tccccgatgt ccaggtgttt gctgatgcta cagtggccca cttttcactt gatggtcatg atggtcttgt ggagccggag actgacgtca tttccaatga cgtctctgtt agccaattag ccaagcaggt tacaacattg gcttccgctg gttgtgatgt ggtgtcgctc aacgacaccc ttgatgggtc ggttggtgtc gccagagatg ccttggactt tgaagggtac accgacgtat cgctaatgag tcgcgctgga aagttcaact cagtatacat ggcacagcat caggctatgt tgggaaccac gccagcgcaa aacgtgcgtg tcgagagcta cctccacgac gtggctattc ccgatgaacc tatcctcaag ggagttcaag acgttgatga cggtgcggat atggtagccg tggagcccgc gactgcattc gtggacgtca ttaggagcct caaggaccgg ctgaggtcgc cagttgtcgc agtgcacacc accggtgaat atcgtgcctt aaaggcagct gcagcagccg gtgtatatga cgaaaaggcc gcggcaatag aggctttacg tggacttcgg cgtgctggcg cggatctcat agtctcaagc ttcgcgaagg acgtggcgca gtggctacta gaggatatgc agaccaatgg gatggaaaat gtagccctgg gcgtctga

Details for BaboA.17087.a.A2.GE34105

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	54kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	89
PERCENT COVERAGE:	61

Validated AA Sequence

Validated NT Sequence

ttttntnnnn tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcagg attctacaat gaatcgaggt ttggtatcgg tgtaacgtat actgatgccg ttgctatacg tcgaaacgat gtgcaacgta tttcacaagc ctcaagatcg tcagacagcg cggctttcgt ttgtgttcca tcgagccaat gggcgcaacc atcaactggt gtgctagaca ctcaacgggg atgtgctact agcaaggtgc tcgccaaacg taaagaagag ggaaatttgg attccactcc cgagggtgta gaatttgatt ctgatgtgga ctacgaggac atttatgacg ctagtgacta tgaatctgat gacgtcgagg gtgaagacgg atcctcttct gagtatgatt ctgatgaaga taccggtaat agtgcgtttg attacaatgc gcgtggagag cttatgcttc caaacctaac ccggttatcg agaacgcgag tgtcgaaaaa ccggcacaag ctattccgcg gtcgcgttct ccacttggct gatttggtat atcctttagt agtcactgac aaggaatctt catcgtctat caaggaggta cctggattgc ggctattatc gattcctgac cttgtaaagg aggttgaaga ggcacgtaac cttggaatca cagctttcat gatacaccca cacatagaca agtcactaaa gagcgagttt ggtgacnagg gtttgaaccc tgatggactt cttccaaggg ctatcgatgc tgtaaaagag gcgttccccg atgtccnntg tttgctgatg ctacagtggc ccacttttca cttgatggtc atgatggtct tgtggagccg gagactgacg tcatttccaa tgacgtctct gttagccaat tagccnagcn ggttacannt tggcttccgc tggnngtgat gnggtgtngc tcannacacc cttgatgggn cggttgnnnn nnccnganat gcctnnnnnt tgaaggnnnn cnacgtnnnn nctagnnncn nnctngnaan tcanctnnnn nnnnnngnnn nnnatcnggn tnnngnannn nnncnnnnnn na

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAGFYNESR FGIGVTYTDA VAIRRNDVQR ISQASRSSDS AAFVCVPSSQ WAQPSTGVLD TQRGCATSKV LAKRKEEGNL DSTPEGVEFD SDVDYEDIYD ASDYESDDVE GEDGSSSEYD SDEDTGNSAF DYNARGELML PNLTRLSRTR VSKNRHKLFR GRVLHLADLV YPLVVTDKES SSSIKEVPGL RLLSIPDLVK EVEEARNLGI TAFMIHPHID KSLKSEFGDE GLNPDGLLPR AIDAVKEAFP DVQVFADATV AHFSLDGHDG LVEPETDVIS NDVSVSQLAK QVTTLASAGC DVVSLNDTLD GSVGVARDAL DFEGYTDVSL MSRAGKFNSV YMAQHQAMLG TTPAQNVRVE SYLHDVAIPD EPILKGVQDV DDGADMVAVE PATAFVDVIR SLKDRLRSPV VAVHTTGEYR ALKAAAAAGV YDEKAAAIEA LRGLRRAGAD LIVSSFAKDV AQWLLEDMQT NGMENVALGV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaggattc tacaatgaat cgaggtttgg tatcggtgta acgtatactg atgccgttgc tatacgtcga aacgatgtgc aacgtatttc acaagcctca agatcgtcag acagcgcggc tttcgtttgt gttccatcga gccaatgggc gcaaccatca actggtgtgc tagacactca acggggatgt gctactagca aggtgctcgc caaacgtaaa gaagagggaa atttggattc cactcccgag ggtgtagaat ttgattctga tgtggactac gaggacattt atgacgctag tgactatgaa tctgatgacg tcgagggtga agacggatcc tcttctgagt atgattctga tgaagatacc ggtaatagtg cgtttgatta caatgcgcgt ggagagctta tgcttccaaa cctaacccgg ttatcgagaa cgcgagtgtc gaaaaaccgg cacaagctat tccgcggtcg cgttctccac ttggctgatt tggtatatcc tttagtagtc actgacaagg aatcttcatc gtctatcaag gaggtacctg gattgcggct attatcgatt cctgaccttg taaaggaggt tgaagaggca cgtaaccttg gaatcacagc tttcatgata cacccacaca tagacaagtc actaaagagc gagtttggtg acgagggttt gaaccctgat ggacttcttc caagggctat cgatgctgta aaagaggcgt tccccgatgt ccaggtgttt gctgatgcta cagtggccca cttttcactt gatggtcatg atggtcttgt ggagccggag actgacgtca tttccaatga cgtctctgtt agccaattag ccaagcaggt tacaacattg gcttccgctg gttgtgatgt ggtgtcgctc aacgacaccc ttgatgggtc ggttggtgtc gccagagatg ccttggactt tgaagggtac accgacgtat cgctaatgag tcgcgctgga aagttcaact cagtatacat ggcacagcat caggctatgt tgggaaccac gccagcgcaa aacgtgcgtg tcgagagcta cctccacgac gtggctattc ccgatgaacc tatcctcaag ggagttcaag acgttgatga cggtgcggat atggtagccg tggagcccgc gactgcattc gtggacgtca ttaggagcct caaggaccgg ctgaggtcgc cagttgtcgc agtgcacacc accggtgaat atcgtgcctt aaaggcagct gcagcagccg gtgtatatga cgaaaaggcc gcggcaatag aggctttacg tggacttcgg cgtgctggcg cggatctcat agtctcaagc ttcgcgaagg acgtggcgca gtggctacta gaggatatgc agaccaatgg gatggaaaat gtagccctgg gcgtctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa