BaboA.10272.a

hexokinase

CENTER ID: BaboA.10272.a
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.10272.a.A1.GE27710 Full length( BaboA.10272.a ) 1 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_I000860
OrthoMCL: OG5_126743
RefSeq: XP_001608748.1
UniProt: A7AXA7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIGEISVIRQ AAEAVEVPYK VTQVLDCDEA VRLDQIIGQL DIPIRYLKDV AQAFYAELVN GLMAHRRHRN LWLPNECSFK MLDSYITHLP TGNEKGCYYA IDFGGSNLRA VRINVTGTGT MERMQSTFSL RHATALRPKG LLDRTATATE LFDHFAKNIG NLMEEAGDVS DPGHIYPVGF TFSFPCTMLS RRNAILLDWT KGFETGRDTT EQVEGRDIGM LMDEAFKRNH VNARVSIILN DTVGTLMSVA YQKPPGYPEC RMGLILGTGF NICYVEHDYL HYGYIGKVVN IECGNFDKLL PTTPVDFEID WYTSNSGRGM MEKLIAGAYL GDIIRRNMLL YLREKAPAKM WNIGTFTSID AAEILNDQSE TFEKAKEIVK ENWDAVLEHH HLAGLRRICE AAFSRSAGLA AAAITATARK TRSYVTNKTT CAVDGSLYVK NQWYRDRLAY YLEKVSRSDL IGSVVMYACD DGSGKGAAIA AAMMAED
NT Sequence
ATGATAGGTG AAATATCTGT TATTCGTCAG GCTGCGGAAG CCGTGGAGGT ACCGTACAAG GTTACTCAAG TGCTGGATTG TGATGAAGCA GTCAGACTTG ACCAAATCAT TGGCCAGCTC GATATACCAA TTAGGTACCT TAAGGATGTC GCTCAGGCAT TTTATGCTGA GTTGGTTAAC GGTCTTATGG CTCACAGAAG GCACCGTAAC TTATGGTTAC CTAATGAGTG TAGCTTCAAG ATGTTAGATT CCTATATTAC TCATTTGCCA ACTGGTAATG AGAAGGGTTG TTACTATGCT ATAGATTTCG GTGGATCTAA CTTGAGAGCC GTCCGTATCA ATGTTACTGG TACTGGTACT ATGGAGAGAA TGCAGTCAAC ATTTAGTTTG AGGCATGCCA CTGCCCTTCG ACCAAAGGGT TTGCTTGACC GTACTGCAAC TGCTACTGAG CTTTTCGACC ATTTTGCGAA GAACATTGGC AATCTTATGG AAGAGGCTGG TGATGTATCA GATCCCGGTC ATATTTACCC TGTTGGCTTT ACCTTTTCAT TCCCGTGCAC TATGTTATCA AGGCGCAATG CCATTCTTCT TGACTGGACT AAGGGTTTTG AGACTGGTCG TGACACAACT GAGCAGGTTG AGGGTCGTGA CATTGGTATG CTTATGGACG AGGCCTTCAA GCGCAACCAT GTTAACGCCA GGGTGTCCAT TATTCTCAAC GACACTGTAG GAACTCTTAT GTCAGTTGCT TATCAGAAGC CTCCTGGTTA CCCTGAATGC CGTATGGGTC TCATTCTTGG TACTGGTTTC AATATATGTT ATGTTGAGCA TGATTACCTT CACTACGGTT ACATTGGTAA GGTTGTCAAC ATTGAGTGTG GTAACTTCGA CAAGCTGTTA CCTACTACTC CAGTGGACTT TGAGATTGAT TGGTACACGT CCAACAGTGG CAGGGGTATG ATGGAGAAGC TCATTGCTGG AGCCTACCTT GGTGACATCA TTAGGAGGAA CATGCTTCTA TATCTACGCG AGAAAGCACC CGCTAAAATG TGGAACATTG GCACTTTCAC TTCTATTGAT GCAGCTGAGA TATTAAACGA CCAGTCTGAA ACCTTTGAGA AGGCTAAGGA GATCGTTAAG GAGAACTGGG ATGCTGTATT GGAGCATCAC CATTTGGCAG GTCTTAGGAG AATCTGTGAG GCTGCATTCT CAAGGTCTGC TGGTCTTGCT GCTGCTGCCA TCACTGCTAC TGCTCGCAAG ACGAGGTCTT ATGTCACCAA CAAGACCACG TGTGCTGTTG ATGGATCACT GTATGTTAAG AACCAGTGGT ACAGGGACAG GCTTGCTTAC TACCTTGAGA AGGTCAGCAG GTCTGACCTT ATAGGATCAG TTGTGATGTA TGCCTGTGAT GATGGATCAG GCAAGGGTGC TGCGATTGCT GCTGCCATGA TGGCGGAGGA C
Details for BaboA.10272.a.A1.GE27710
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 56kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MIGEISVIRQ AAEAVEVPYK VTQVLDCDEA VRLDQIIGQL DIPIRYLKDV AQAFYAELVN GLMAHRRHRN LWLPNECSFK MLDSYITHLP TGNEKGCYYA IDFGGSNLRA VRINVTGTGT MERMQSTFSL RHATALRPKG LLDRTATATE LFDHFAKNIG NLMEEAGDVS DPGHIYPVGF TFSFPCTMLS RRNAILLDWT KGFETGRDTT EQVEGRDIGM LMDEAFKRNH VNARVSIILN DTVGTLMSVA YQKPPGYPEC RMGLILGTGF NICYVEHDYL HYGYIGKVVN IECGNFDKLL PTTPVDFEID WYTSNSGRGM MEKLIAGAYL GDIIRRNMLL YLREKAPAKM WNIGTFTSID AAEILNDQSE TFEKAKEIVK ENWDAVLEHH HLAGLRRICE AAFSRSAGLA AAAITATARK TRSYVTNKTT CAVDGSLYVK NQWYRDRLAY YLEKVSRSDL IGSVVMYACD DGSGKGAAIA AAMMA
Validated NT Sequence
ccgccatcat ggcagcagca atcgcagcac ccttgcctga tccatcatca caggcataca tcacaactga tcctataagg tcagacctgc tgaccttctc aaggtagtaa gcaagcctgt ccctgtacca ctggttctta acatacagtg atccatcaac agcacacgtg gtcttgttgg tgacataaga cctcgtcttg cgagcagtag cagtgatggc agcagcagca agaccagcag accttgagaa tgcagcctca cagattctcc taagacctgc caaatggtga tgctccaata cagcatccca gttctcctta acgatctcct tagccttctc aaaggtttca gactggtcgt ttaatatctc agctgcatca atagaagtga aagtgccaat gttccacatt ttagcgggtg ctttctcgcg tagatataga agcatgttcc tcctaatgat gtcaccaagg taggctccag caatgagctt ctccatcata cccctgccac tgttggacgt gtaccaatca atctcaaagt ccactggagt agtaggtaac agcttgtcga agttaccaca ctcaatgttg acaaccttac caatgtaacc gtagtgaagg taatcatgct caacataaca tatattgaaa ccagtaccaa gaatgagacc catacggcat tcagggtaac caggaggctt ctgataagca actgacataa gagttcctac agtgtcgttg agaataatgg acaccctggc gttaacatgg ttgcgcttga aggcctcgtc cataagcata ccaatgtcac gaccctcaac ctgctcagtt gtgtcacgac cagtctcaaa acccttagtc cagtcaagaa gaatggcatt gcgccttgat aacatagtgc acgggaatga aaaggtaaag ccaacagggt aaatatgacc gggatctgat acatcaccag cctcttccat aagattgcca atgttcttcg caaaatggtc gaaaagctca gtagcagttg cagtacggtc aagcaaaccc tttggtcgaa gggcagtggc atgcctcaaa ctaaatgttg actgcattct ctccatagta ccagtaccag taacattgat acggacggct ctcaagttag atccaccgaa atctatagca tagtaacaac ccttctcatt accagttggc aaatgagtaa tataggaatc taacatcttg aagctacact cattaggtaa ccataagtta cggtgccttc tgtgagccat aagaccgtta accaactcag cataaaatgc ctgagcgaca tccttaaggt acctaattgg tatatcgagc tggccaatga tttggtcaag tctgactgct tcatcacaat ccagcacttg agtaaccttg tacggtacct ccacggcttc cgcagcctga cgaataacag atatttcacc tatcatcgaa ccaggaccct gggtctgagc ttccagggta ccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIGEISVIR QAAEAVEVPY KVTQVLDCDE AVRLDQIIGQ LDIPIRYLKD VAQAFYAELV NGLMAHRRHR NLWLPNECSF KMLDSYITHL PTGNEKGCYY AIDFGGSNLR AVRINVTGTG TMERMQSTFS LRHATALRPK GLLDRTATAT ELFDHFAKNI GNLMEEAGDV SDPGHIYPVG FTFSFPCTML SRRNAILLDW TKGFETGRDT TEQVEGRDIG MLMDEAFKRN HVNARVSIIL NDTVGTLMSV AYQKPPGYPE CRMGLILGTG FNICYVEHDY LHYGYIGKVV NIECGNFDKL LPTTPVDFEI DWYTSNSGRG MMEKLIAGAY LGDIIRRNML LYLREKAPAK MWNIGTFTSI DAAEILNDQS ETFEKAKEIV KENWDAVLEH HHLAGLRRIC EAAFSRSAGL AAAAITATAR KTRSYVTNKT TCAVDGSLYV KNQWYRDRLA YYLEKVSRSD LIGSVVMYAC DDGSGKGAAI AAAMMAED
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gataggtgaa atatctgtta ttcgtcaggc tgcggaagcc gtggaggtac cgtacaaggt tactcaagtg ctggattgtg atgaagcagt cagacttgac caaatcattg gccagctcga tataccaatt aggtacctta aggatgtcgc tcaggcattt tatgctgagt tggttaacgg tcttatggct cacagaaggc accgtaactt atggttacct aatgagtgta gcttcaagat gttagattcc tatattactc atttgccaac tggtaatgag aagggttgtt actatgctat agatttcggt ggatctaact tgagagccgt ccgtatcaat gttactggta ctggtactat ggagagaatg cagtcaacat ttagtttgag gcatgccact gcccttcgac caaagggttt gcttgaccgt actgcaactg ctactgagct tttcgaccat tttgcgaaga acattggcaa tcttatggaa gaggctggtg atgtatcaga tcccggtcat atttaccctg ttggctttac cttttcattc ccgtgcacta tgttatcaag gcgcaatgcc attcttcttg actggactaa gggttttgag actggtcgtg acacaactga gcaggttgag ggtcgtgaca ttggtatgct tatggacgag gccttcaagc gcaaccatgt taacgccagg gtgtccatta ttctcaacga cactgtagga actcttatgt cagttgctta tcagaagcct cctggttacc ctgaatgccg tatgggtctc attcttggta ctggtttcaa tatatgttat gttgagcatg attaccttca ctacggttac attggtaagg ttgtcaacat tgagtgtggt aacttcgaca agctgttacc tactactcca gtggactttg agattgattg gtacacgtcc aacagtggca ggggtatgat ggagaagctc attgctggag cctaccttgg tgacatcatt aggaggaaca tgcttctata tctacgcgag aaagcacccg ctaaaatgtg gaacattggc actttcactt ctattgatgc agctgagata ttaaacgacc agtctgaaac ctttgagaag gctaaggaga tcgttaagga gaactgggat gctgtattgg agcatcacca tttggcaggt cttaggagaa tctgtgaggc tgcattctca aggtctgctg gtcttgctgc tgctgccatc actgctactg ctcgcaagac gaggtcttat gtcaccaaca agaccacgtg tgctgttgat ggatcactgt atgttaagaa ccagtggtac agggacaggc ttgcttacta ccttgagaag gtcagcaggt ctgaccttat aggatcagtt gtgatgtatg cctgtgatga tggatcaggc aagggtgctg cgattgctgc tgccatgatg gcggaggaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a