BaboA.01644.a

GTP-binding nuclear protein Ran

CENTER ID: BaboA.01644.a
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.01644.a.MB1.GE30401 Full length( BaboA.01644.a ) 1 211
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_IV010920
RefSeq: XP_001611013.1
UniProt: A7ASC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEEMPQFKL LLVGDGGVGK TTLVKRHLTG EFEKKYIPTL GVEVHPLKFR TNCGGIQFNA WDTAGQEKYG GLRDGYYIKG ECAIIMFDVT SRITYRNVPN WHRDIVRVCD NIPMVLCGNK ADVKERQVKA GHIQFHRKRN LQYYDLSARS NFNFERPFLW LARRLLNQPQ LVFVGECAKE PEFRIDPQLA QECERNLEAA ANVAIDDDGD L
NT Sequence
ATGGCGGAGG AGATGCCACA ATTCAAATTG CTCCTTGTGG GAGATGGTGG TGTCGGTAAA ACTACCTTAG TAAAGCGTCA TTTGACTGGA GAGTTTGAAA AAAAGTACAT TCCCACGTTA GGTGTTGAGG TGCATCCTTT AAAGTTCCGT ACTAATTGCG GTGGTATTCA ATTCAACGCA TGGGATACTG CTGGTCAGGA GAAATACGGT GGTCTTCGTG ACGGTTACTA CATCAAGGGT GAGTGCGCCA TTATTATGTT CGACGTGACT AGCCGTATCA CATACCGCAA CGTACCTAAC TGGCATCGTG ACATCGTCCG TGTTTGTGAC AACATTCCAA TGGTTTTGTG TGGAAACAAG GCTGACGTCA AGGAGCGTCA AGTTAAGGCC GGCCACATTC AATTCCACCG CAAGAGGAAC TTGCAATATT ATGACTTATC AGCTCGTTCC AACTTCAACT TCGAGCGTCC ATTTTTGTGG CTCGCAAGAC GTTTGCTTAA CCAGCCTCAA TTGGTCTTCG TTGGAGAATG CGCGAAAGAG CCCGAGTTTA GGATTGACCC TCAGTTGGCT CAGGAGTGTG AGCGTAACCT AGAGGCTGCC GCCAACGTTG CTATTGACGA CGATGGTGAC CTT
Details for BaboA.01644.a.MB1.GE30401
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 39
Validated AA Sequence
MAEEMPQFKL LLVGDGGVGK TTLVKRHLTG EFEKKYIPTL GVEVHPLKFR TNCGGIQFNA WDTAGQEKYG GLRDGYYIKG ECAIIMFDVT SRITYRNVPN WHRDIVRVCG NIPMVLCGNK ADVKERQVKA GHIQFHRKRN LQYYDLSARS NFNFERPFLW LARRLLNQPQ LVFVGECAKE PEFRIDPQLA XECERNXEAA AXVXXXDXXT FNKQHEQVLX XSFSRIXLLT KPERKLXWLX XXXXXXXX
Validated NT Sequence
ggnnctcaga cccngggtcc tggttcgatg gcggaggaga tgccacaatt caaattgctc cttgtgggag atggtggtgt cggtaaaact accttagtaa agcgtcattt gactggagag tttgaaaaaa agtacattcc cacgttaggt gttgaggtgc atcctttaaa gttccgtact aattgcggtg gtattcaatt caacgcatgg gatactgctg gtcaggagaa atacggtggt cttcgtgacg gttactacat caagggtgag tgcgccatta ttatgttcga cgtgactagc cgtatcacat accgcaacgt acctaactgg catcgtgaca tcgtccgtgt ttgtggcaac attccaatgg ttttgtgtgg aaacaaggct gacgtcaagg agcgtcaagt taaggccggc cacattcaat tccaccgcaa gaggaacttg caatattatg acttatcagc tcgttccaac ttcaacttcg agcgtccatt tttgtggctc gcaagacgtt tgcttaacca gcctcaattg gtcttcgttg gagaatgcgc gaaggagccc gagtttagga ttgaccctca gttggctcan gagtgtgagc gtaaccnaga ggctgccgcn nacgttncta tnnacgacna nnngaccttt aataaacagc acgaacaagt tctgcanncc agcttctcga ggatccngct gctaacaaag cccgaaagga agctganttg gctgcngncn ncncnnnnnn nnnnnnnnna aaaa
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MAEEMPQFKL LLVGDGGVGK TTLVKRHLTG EFEKKYIPTL GVEVHPLKFR TNCGGIQFNA WDTAGQEKYG GLRDGYYIKG ECAIIMFDVT SRITYRNVPN WHRDIVRVCD NIPMVLCGNK ADVKERQVKA GHIQFHRKRN LQYYDLSARS NFNFERPFLW LARRLLNQPQ LVFVGECAKE PEFRIDPQLA QECERNLEAA ANVAIDDDGD L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggc ggaggagatg ccacaattca aattgctcct tgtgggagat ggtggtgtcg gtaaaactac cttagtaaag cgtcatttga ctggagagtt tgaaaaaaag tacattccca cgttaggtgt tgaggtgcat cctttaaagt tccgtactaa ttgcggtggt attcaattca acgcatggga tactgctggt caggagaaat acggtggtct tcgtgacggt tactacatca agggtgagtg cgccattatt atgttcgacg tgactagccg tatcacatac cgcaacgtac ctaactggca tcgtgacatc gtccgtgttt gtgacaacat tccaatggtt ttgtgtggaa acaaggctga cgtcaaggag cgtcaagtta aggccggcca cattcaattc caccgcaaga ggaacttgca atattatgac ttatcagctc gttccaactt caacttcgag cgtccatttt tgtggctcgc aagacgtttg cttaaccagc ctcaattggt cttcgttgga gaatgcgcga aagagcccga gtttaggatt gaccctcagt tggctcagga gtgtgagcgt aacctagagg ctgccgccaa cgttgctatt gacgacgatg gtgaccttta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a