CENTER ID: | BaboA.01620.a |
ORGANISM: | Babesia bovis T2Bo |
ASSOCIATED DISEASE: | Babesiosis (atypical) |
CURRENT STATUS: | diffraction |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
I |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
BaboA.01620.a.A2.GE29393 | corrected N-term | 33 | 199 | ||
BaboA.01620.a.AE2.GE43216 | corrected N-term | 33 | 199 | ||
BaboA.01620.a.B1.GE26932 | Full length( BaboA.01620.a ) | 1 | 199 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
BaboA.01620.a.A2.PW29290 | corrected N-term | 33 | 199 |
RESOURCE | REFERENCE ID |
---|---|
EuPathDB: | PiroplasmaDB:BBOV_IV002810 |
OrthoMCL: | OG5_130616 |
RefSeq: | XP_001609446.1 |
UniProt: | A7AVQ2 |
HARVESTED ON: | 5/27/2010 |
SEQUENCED ON: | 6/24/2010 |
EXPECTED MW: | 20kDa |
OBSERVED MW: | 19kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 6/12/2019 |
SEQUENCED ON: | 6/18/2019 |
EXPECTED MW: | 19kDa |
OBSERVED MW: | 19kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 101 |
HARVESTED ON: | 6/18/2009 |
SEQUENCED ON: | 7/15/2009 |
EXPECTED MW: | 22kDa |
OBSERVED MW: | 23kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with sequence variation |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 10/18/2010 |
CONCENTRATION: | 23.08mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 5 |
VIAL VOLUME: | 200µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |