BaboA.01523.b

Putative uncharacterized protein (PRMT7 homologue)

CENTER ID: BaboA.01523.b
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.01523.b.D12.GE082212a full length 1 527
BaboA.01523.b.D13.GE082212a full length 161 537
BaboA.01523.b.D16.GE082212a full length 154 527
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.01523.b.D12.PD00114 full length 1 527
BaboA.01523.b.D16.PD00115 full length 154 527
BaboA.01523.b.D13.PD00163 full length 161 537
External Resources
RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_III002510
OrthoMCL: OG5_147460
RefSeq: XP_001611384.1
UniProt: A7AMN2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTESDDSG SSGSEMESVW EGDGVAYSIF DDFQSPYAVE VYRHMASKFG FCHSILGKGT LHRIALVTYL EREKARGSDP LESYRRVCAD PSIVLDHLDE YINPPFSNEK LIWDFLESSD EEDDEGVFQR HLHLERGTKT AKSQTKKCIQ DDDYFKGYKN LAIHREMVND RVRTGAYESF IRLNKSLFKD KVVLDVGSGC GILSLFAAKA GARLVVGIDN CDNMIKMSEN HARINGIGNV QFLHGKVEDS DLVYSGGVVQ FKRDDLPCEP FKCDILISEW MGYALLYENM LSSVLFARDR YLISGGLMVP SKVKLGLFAV DMYDDIKSKL NEWDERKYGL ILDGLRYEAH ELLKEPVVEV VDPSRIVSDA SGICILDLGR LNVQDLGKAH EFQVAIHDGC KCSSLALYFD CIFEPVASDS ANVIQSLKGK RFHTLESYRE VLGIDELTSY VTSPDYPHPE EPMSTAVVMS TRADQPPTHW KQVILHLTDH NNMPVAIQGA IGGKLSLLNN SSNCRWLDIL FQPDDTSLLG ECYYKLM
NT Sequence
atgagctcca ctgaatctga tgactctgga tcgtctggat ccgagatgga gtcagtttgg gaaggtgacg gtgttgctta cagtatcttt gatgacttcc aatccccgta tgccgtggaa gtatatcggc acatggcatc taagtttggt ttttgtcata gtatattggg taagggcaca ttacatcgta tagctttggt aacctacctt gagcgtgaga aggctcgtgg ttcagatcct ttggaatcct accgtcgtgt ttgtgcggat ccttctatag tactcgatca tttagatgag tacataaacc ctccatttag caatgagaag ttgatttggg actttttgga atccagtgat gaggaggatg acgagggtgt gttccaacgt caccttcatt tagagcgtgg tactaaaact gcaaaatcgc aaaccaaaaa gtgcatccag gatgatgact atttcaaggg ctacaaaaac cttgctatac accgtgagat ggtgaacgat cgcgttagga cgggtgctta tgagtctttc attaggctca acaaatcgct tttcaaggac aaggtggttc ttgatgttgg ttctggttgc ggcatactat cgctatttgc cgctaaggct ggtgctcgtt tggttgttgg cattgacaat tgtgacaaca tgattaagat gtctgaaaat catgctcgta tcaacggtat tggtaatgtc cagtttttac atgggaaggt tgaggattcc gatctagttt actctggcgg cgttgtgcag ttcaagcgtg acgaccttcc ttgtgaaccc tttaagtgtg acattcttat ttcggaatgg atgggttatg ctcttttata tgaaaacatg ctttcttcgg tgttatttgc tcgtgataga tatcttatat cgggtggtct gatggttcct agtaaggtta agttagggct ctttgctgtg gacatgtacg acgacataaa gtctaagttg aacgaatggg atgaacgtaa atatggactc atccttgatg gtttgcgtta cgaggcacat gaactgctta aggagccagt cgttgaagtg gtcgacccat cgcgtattgt cagcgatgcg agtggcattt gcatcctaga tttgggacgc ttgaatgtgc aagatttggg caaggcgcat gaattccagg tagccataca cgacggctgc aaatgctcat ctctagctct atactttgac tgcatctttg agcctgttgc tagtgacagt gccaatgtca tacaatcgct aaagggcaaa cgcttccaca cactggaatc ttatcgggaa gttcttggta ttgatgaact cacgtcatac gtaacttcac ctgattatcc acaccccgag gaacctatga gcactgctgt tgtgatgtca acaagggctg accagccgcc tactcattgg aagcaagtta tattacatct tacggatcac aataacatgc ctgtggccat acaaggcgcc attggcggca aactatcgct tcttaacaat tccagcaatt gcagatggtt ggacatatta ttccagccgg atgacacctc gttgctgggc gagtgttact ataagctgat gtag
Details for BaboA.01523.b.D12.GE082212a
HARVESTED ON: 2/19/2010
SEQUENCED ON: 9/18/2012
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 73
PERCENT COVERAGE: 60
Validated AA Sequence
MGHHHHHHSG EVXXEVKXXT HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMSST ESDDSGSSGS EMESVWEGDG VAYSIFDDFQ SPYAVEVYRH MASKFGFCHS ILGKGTLHRI ALVTYLEREK ARGSDPLESY RRVCADPSIV LDHLDEYINP PFSNEKLIWD FLESSDEEDD EGVFQRXLHL ERGTKTAKSQ TKKCIQDDDY XXXYKNLAIH REMVNDRVRT XAYESFIXLX XLCLXXXXSG RWXXXXXCLX XCXXXSSXXX XXCXXDXXXX SXXHXXXXXX CXXXXXXTXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
ctnnnnnnnt ttnnttaact ttaagaagga ganataccat gggccaccac caccaccacc atagcggcga agtnnanncg gaagtgaagn ngganaccca catcaacctg aaggttagcg acggtagcag cgaaatcttc ttcaagatta agaagaccac cccgctgcgt cgcctgatgg aagcattcgc taaacgccag ggcaaggaaa tggattccct gcgctttctg tacgacggta tccgtattca ggcagaccag actccggaag atctggacat ggaagataac gacattatcg aagcacaccg tgaacaaatc ggtggaatgt cttctactga atctgacgac agcggcagct ctggttctga aatggaaagc gtgtgggaag gtgacggcgt agcttacagc atctttgacg actttcagtc tccgtacgct gttgaagttt atcgtcacat ggctagcaaa tttggcttct gccactccat tttaggcaaa ggtactctgc atcgtatcgc cctggtgacc tacctggaac gtgagaaggc tcgcggtagc gacccgctgg aatcctatcg tcgtgtctgc gctgatccgt ctatagttct ggatcacctg gacgagtaca tcaacccgcc tttctctaac gagaagttaa tctgggactt cctggagtct tctgacgaag aggacgacga gggcgtgttc cagcgccncc ttcacttaga gcgcggtact aagaccgcta agtcccagac caagaagtgt atccaagacg atgactacnt caanngctac aaaaacttag ctattcatcg cgaaatggtt aacgaccgcg tacgcaccng tgcgtatgaa tctttcattc ntctcannan tctctgttta aananaanta nttctggacg ttggtnctgn tnnngtatnt gtctcnntnn ctgcnnnnng nnctcgtcnn nntnnnnttg nnantgcnan anagatannn nannnanatc anncnnncat nacgnatnnn nnntncantg cangnnnntn aannnnnnna ctnnnnnnnn ngncnnnnnn tnnnnnannn nnnncnnnnn nnnnnnntna nnnnnnnnnn nnnnnnnnnn nnnngnangg nnnnnnncnn nnnnnnnnnn nnnnnaaaan nnnnnnnna
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMSST ESDDSGSSGS EMESVWEGDG VAYSIFDDFQ SPYAVEVYRH MASKFGFCHS ILGKGTLHRI ALVTYLEREK ARGSDPLESY RRVCADPSIV LDHLDEYINP PFSNEKLIWD FLESSDEEDD EGVFQRHLHL ERGTKTAKSQ TKKCIQDDDY FKGYKNLAIH REMVNDRVRT GAYESFIRLN KSLFKDKVVL DVGSGCGILS LFAAKAGARL VVGIDNCDNM IKMSENHARI NGIGNVQFLH GKVEDSDLVY SGGVVQFKRD DLPCEPFKCD ILISEWMGYA LLYENMLSSV LFARDRYLIS GGLMVPSKVK LGLFAVDMYD DIKSKLNEWD ERKYGLILDG LRYEAHELLK EPVVEVVDPS RIVSDASGIC ILDLGRLNVQ DLGKAHEFQV AIHDGCKCSS LALYFDCIFE PVASDSANVI QSLKGKRFHT LESYREVLGI DELTSYVTSP DYPHPEEPMS TAVVMSTRAD QPPTHWKQVI LHLTDHNNMP VAIQGAIGGK LSLLNNSSNC RWLDILFQPD DTS
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gtcttctact gaatctgacg acagcggcag ctctggttct gaaatggaaa gcgtgtggga aggtgacggc gtagcttaca gcatctttga cgactttcag tctccgtacg ctgttgaagt ttatcgtcac atggctagca aatttggctt ctgccactcc attttaggca aaggtactct gcatcgtatc gccctggtga cctacctgga acgtgagaag gctcgcggta gcgacccgct ggaatcctat cgtcgtgtct gcgctgatcc gtctatagtt ctggatcacc tggacgagta catcaacccg cctttctcta acgagaagtt aatctgggac ttcctggagt cttctgacga agaggacgac gagggcgtgt tccagcgcca ccttcactta gagcgcggta ctaagaccgc taagtcccag accaagaagt gtatccaaga cgatgactac ttcaaaggct acaaaaactt agctattcat cgcgaaatgg ttaacgaccg cgtacgcacc ggtgcgtatg aatctttcat tcgtctcaac aagtctctgt ttaaagataa agtagttctg gacgttggtt ctggttgtgg tattctgtct ctgttcgctg ctaaggcagg cgctcgtctg gttgtcggca ttgacaactg cgacaatatg attaagatga gcgagaatca cgcacgcatc aacggtatcg gcaacgtgca gttcttgcat ggtaaagttg aagactctga cctggtttac tctggcggcg tagttcaatt caaacgtgat gacctgccgt gcgagccgtt caaatgcgac atcctgatca gcgagtggat gggctacgct ctgctctatg aaaatatgct gagctctgtg ctcttcgccc gcgatcgcta cctgatcagc ggcggtctga tggttccatc taaagtaaag ttaggcctct tcgcagttga catgtatgac gacattaagt ctaaactgaa cgaatgggat gaacgtaagt atggcctgat cctcgatggt ctgcgctacg aagctcatga actgctgaaa gaaccggtcg tagaagtggt ggacccttcc cgcattgttt ctgacgcctc cggtatctgc atcctcgatc tgggccgtct gaacgtacag gacttaggca aagcacatga atttcaggta gctattcacg acggttgtaa atgctcttcc ctggcccttt actttgactg catctttgaa ccggtagctt ctgatagcgc taacgtgatc cagtccctga aaggtaaacg tttccacact ctcgagtcct accgcgaagt tctgggtatc gacgaactga cttcctatgt gacctctccg gactaccctc accctgaaga accaatgagc accgctgttg ttatgtctac ccgtgccgac cagcctccga cccattggaa gcaggttatc ctgcatctga ccgaccacaa caacatgccg gttgcaatcc agggcgctat tggcggcaag ttatccctgc tcaacaactc ttccaactgc cgctggctgg atattctgtt tcagcctgac gacacctcct aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for BaboA.01523.b.D13.GE082212a
HARVESTED ON: data unavailable
SEQUENCED ON: 9/18/2012
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGLAIH REMVNDRVRT GAYESFIRLN KSLFKDKVVL DVGSGCGILS LFAAKAGARL VVGIDNCDNM IKMSENHARI NGIGNVQFLH GKVEDSDLVY SGGVVQFKRD DLPCEPFKCD ILISEWMGYA LLYENMLSSV LFARDRYLIS GGLMVPSKVK LGLFAVDMYD DIKSKLNEWD ERKYGLILDG LRYEAHELLK EPVVEVVDPS RIVSDASGIC ILDLGRLNVQ DLGKAHEFQV AIHDGCKCSS LALYFDCIFE PVASDSANVI QSLKGKRFHT LESYREVLGI DELTSYVTSP DYPHPEEPMS TAVVMSTRAD QPPTHWKQVI LHLTDHNNMP VAIQGAIGGK LSLLNNSSNC RWLDILFQPD DTSLLGECYY KLM
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggatt agctattcat cgcgaaatgg ttaacgaccg cgtacgcacc ggtgcgtatg aatctttcat tcgtctcaac aagtctctgt ttaaagataa agtagttctg gacgttggtt ctggttgtgg tattctgtct ctgttcgctg ctaaggcagg cgctcgtctg gttgtcggca ttgacaactg cgacaatatg attaagatga gcgagaatca cgcacgcatc aacggtatcg gcaacgtgca gttcttgcat ggtaaagttg aagactctga cctggtttac tctggcggcg tagttcaatt caaacgtgat gacctgccgt gcgagccgtt caaatgcgac atcctgatca gcgagtggat gggctacgct ctgctctatg aaaatatgct gagctctgtg ctcttcgccc gcgatcgcta cctgatcagc ggcggtctga tggttccatc taaagtaaag ttaggcctct tcgcagttga catgtatgac gacattaagt ctaaactgaa cgaatgggat gaacgtaagt atggcctgat cctcgatggt ctgcgctacg aagctcatga actgctgaaa gaaccggtcg tagaagtggt ggacccttcc cgcattgttt ctgacgcctc cggtatctgc atcctcgatc tgggccgtct gaacgtacag gacttaggca aagcacatga atttcaggta gctattcacg acggttgtaa atgctcttcc ctggcccttt actttgactg catctttgaa ccggtagctt ctgatagcgc taacgtgatc cagtccctga aaggtaaacg tttccacact ctcgagtcct accgcgaagt tctgggtatc gacgaactga cttcctatgt gacctctccg gactaccctc accctgaaga accaatgagc accgctgttg ttatgtctac ccgtgccgac cagcctccga cccattggaa gcaggttatc ctgcatctga ccgaccacaa caacatgccg gttgcaatcc agggcgctat tggcggcaag ttatccctgc tcaacaactc ttccaactgc cgctggctgg atattctgtt tcagcctgac gacacctccc tgctgggtga atgctactac aaactgatgt aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for BaboA.01523.b.D16.GE082212a
HARVESTED ON: 2/19/2010
SEQUENCED ON: 9/18/2012
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 33
PERCENT COVERAGE: 21
Validated AA Sequence
MGHHHXXXXX AXXXXXXXXX XXAAAAXXXL XXXSRLRRPP RCXXXXXXXA KRXGKEMDSL RFLYDGIXIX AXXTPXXXXX XXXXXXXXXX EXXXGYFXG
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYFKG YKNLAIHREM VNDRVRTGAY ESFIRLNKSL FKDKVVLDVG SGCGILSLFA AKAGARLVVG IDNCDNMIKM SENHARINGI GNVQFLHGKV EDSDLVYSGG VVQFKRDDLP CEPFKCDILI SEWMGYALLY ENMLSSVLFA RDRYLISGGL MVPSKVKLGL FAVDMYDDIK SKLNEWDERK YGLILDGLRY EAHELLKEPV VEVVDPSRIV SDASGICILD LGRLNVQDLG KAHEFQVAIH DGCKCSSLAL YFDCIFEPVA SDSANVIQSL KGKRFHTLES YREVLGIDEL TSYVTSPDYP HPEEPMSTAV VMSTRADQPP THWKQVILHL TDHNNMPVAI QGAIGGKLSL LNNSSNCRWL DILFQPDDTS
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata cttcaaaggc tacaaaaact tagctattca tcgcgaaatg gttaacgacc gcgtacgcac cggtgcgtat gaatctttca ttcgtctcaa caagtctctg tttaaagata aagtagttct ggacgttggt tctggttgtg gtattctgtc tctgttcgct gctaaggcag gcgctcgtct ggttgtcggc attgacaact gcgacaatat gattaagatg agcgagaatc acgcacgcat caacggtatc ggcaacgtgc agttcttgca tggtaaagtt gaagactctg acctggttta ctctggcggc gtagttcaat tcaaacgtga tgacctgccg tgcgagccgt tcaaatgcga catcctgatc agcgagtgga tgggctacgc tctgctctat gaaaatatgc tgagctctgt gctcttcgcc cgcgatcgct acctgatcag cggcggtctg atggttccat ctaaagtaaa gttaggcctc ttcgcagttg acatgtatga cgacattaag tctaaactga acgaatggga tgaacgtaag tatggcctga tcctcgatgg tctgcgctac gaagctcatg aactgctgaa agaaccggtc gtagaagtgg tggacccttc ccgcattgtt tctgacgcct ccggtatctg catcctcgat ctgggccgtc tgaacgtaca ggacttaggc aaagcacatg aatttcaggt agctattcac gacggttgta aatgctcttc cctggccctt tactttgact gcatctttga accggtagct tctgatagcg ctaacgtgat ccagtccctg aaaggtaaac gtttccacac tctcgagtcc taccgcgaag ttctgggtat cgacgaactg acttcctatg tgacctctcc ggactaccct caccctgaag aaccaatgag caccgctgtt gttatgtcta cccgtgccga ccagcctccg acccattgga agcaggttat cctgcatctg accgaccaca acaacatgcc ggttgcaatc cagggcgcta ttggcggcaa gttatccctg ctcaacaact cttccaactg ccgctggctg gatattctgt ttcagcctga cgacacctcc taaaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for BaboA.01523.b.D12.PD00114
PURIFICATION DATe: 3/7/2010
CONCENTRATION: 15.88mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 73
PERCENT COVERAGE: 60
Protocol Notes
notes unavailable
Validated AA Sequence
MGHHHHHHSG EVXXEVKXXT HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMSST ESDDSGSSGS EMESVWEGDG VAYSIFDDFQ SPYAVEVYRH MASKFGFCHS ILGKGTLHRI ALVTYLEREK ARGSDPLESY RRVCADPSIV LDHLDEYINP PFSNEKLIWD FLESSDEEDD EGVFQRXLHL ERGTKTAKSQ TKKCIQDDDY XXXYKNLAIH REMVNDRVRT XAYESFIXLX XLCLXXXXSG RWXXXXXCLX XCXXXSSXXX XXCXXDXXXX SXXHXXXXXX CXXXXXXTXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
ctnnnnnnnt ttnnttaact ttaagaagga ganataccat gggccaccac caccaccacc atagcggcga agtnnanncg gaagtgaagn ngganaccca catcaacctg aaggttagcg acggtagcag cgaaatcttc ttcaagatta agaagaccac cccgctgcgt cgcctgatgg aagcattcgc taaacgccag ggcaaggaaa tggattccct gcgctttctg tacgacggta tccgtattca ggcagaccag actccggaag atctggacat ggaagataac gacattatcg aagcacaccg tgaacaaatc ggtggaatgt cttctactga atctgacgac agcggcagct ctggttctga aatggaaagc gtgtgggaag gtgacggcgt agcttacagc atctttgacg actttcagtc tccgtacgct gttgaagttt atcgtcacat ggctagcaaa tttggcttct gccactccat tttaggcaaa ggtactctgc atcgtatcgc cctggtgacc tacctggaac gtgagaaggc tcgcggtagc gacccgctgg aatcctatcg tcgtgtctgc gctgatccgt ctatagttct ggatcacctg gacgagtaca tcaacccgcc tttctctaac gagaagttaa tctgggactt cctggagtct tctgacgaag aggacgacga gggcgtgttc cagcgccncc ttcacttaga gcgcggtact aagaccgcta agtcccagac caagaagtgt atccaagacg atgactacnt caanngctac aaaaacttag ctattcatcg cgaaatggtt aacgaccgcg tacgcaccng tgcgtatgaa tctttcattc ntctcannan tctctgttta aananaanta nttctggacg ttggtnctgn tnnngtatnt gtctcnntnn ctgcnnnnng nnctcgtcnn nntnnnnttg nnantgcnan anagatannn nannnanatc anncnnncat nacgnatnnn nnntncantg cangnnnntn aannnnnnna ctnnnnnnnn ngncnnnnnn tnnnnnannn nnnncnnnnn nnnnnnntna nnnnnnnnnn nnnnnnnnnn nnnngnangg nnnnnnncnn nnnnnnnnnn nnnnnaaaan nnnnnnnna
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMSST ESDDSGSSGS EMESVWEGDG VAYSIFDDFQ SPYAVEVYRH MASKFGFCHS ILGKGTLHRI ALVTYLEREK ARGSDPLESY RRVCADPSIV LDHLDEYINP PFSNEKLIWD FLESSDEEDD EGVFQRHLHL ERGTKTAKSQ TKKCIQDDDY FKGYKNLAIH REMVNDRVRT GAYESFIRLN KSLFKDKVVL DVGSGCGILS LFAAKAGARL VVGIDNCDNM IKMSENHARI NGIGNVQFLH GKVEDSDLVY SGGVVQFKRD DLPCEPFKCD ILISEWMGYA LLYENMLSSV LFARDRYLIS GGLMVPSKVK LGLFAVDMYD DIKSKLNEWD ERKYGLILDG LRYEAHELLK EPVVEVVDPS RIVSDASGIC ILDLGRLNVQ DLGKAHEFQV AIHDGCKCSS LALYFDCIFE PVASDSANVI QSLKGKRFHT LESYREVLGI DELTSYVTSP DYPHPEEPMS TAVVMSTRAD QPPTHWKQVI LHLTDHNNMP VAIQGAIGGK LSLLNNSSNC RWLDILFQPD DTS
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GTCTTCTACT GAATCTGACG ACAGCGGCAG CTCTGGTTCT GAAATGGAAA GCGTGTGGGA AGGTGACGGC GTAGCTTACA GCATCTTTGA CGACTTTCAG TCTCCGTACG CTGTTGAAGT TTATCGTCAC ATGGCTAGCA AATTTGGCTT CTGCCACTCC ATTTTAGGCA AAGGTACTCT GCATCGTATC GCCCTGGTGA CCTACCTGGA ACGTGAGAAG GCTCGCGGTA GCGACCCGCT GGAATCCTAT CGTCGTGTCT GCGCTGATCC GTCTATAGTT CTGGATCACC TGGACGAGTA CATCAACCCG CCTTTCTCTA ACGAGAAGTT AATCTGGGAC TTCCTGGAGT CTTCTGACGA AGAGGACGAC GAGGGCGTGT TCCAGCGCCA CCTTCACTTA GAGCGCGGTA CTAAGACCGC TAAGTCCCAG ACCAAGAAGT GTATCCAAGA CGATGACTAC TTCAAAGGCT ACAAAAACTT AGCTATTCAT CGCGAAATGG TTAACGACCG CGTACGCACC GGTGCGTATG AATCTTTCAT TCGTCTCAAC AAGTCTCTGT TTAAAGATAA AGTAGTTCTG GACGTTGGTT CTGGTTGTGG TATTCTGTCT CTGTTCGCTG CTAAGGCAGG CGCTCGTCTG GTTGTCGGCA TTGACAACTG CGACAATATG ATTAAGATGA GCGAGAATCA CGCACGCATC AACGGTATCG GCAACGTGCA GTTCTTGCAT GGTAAAGTTG AAGACTCTGA CCTGGTTTAC TCTGGCGGCG TAGTTCAATT CAAACGTGAT GACCTGCCGT GCGAGCCGTT CAAATGCGAC ATCCTGATCA GCGAGTGGAT GGGCTACGCT CTGCTCTATG AAAATATGCT GAGCTCTGTG CTCTTCGCCC GCGATCGCTA CCTGATCAGC GGCGGTCTGA TGGTTCCATC TAAAGTAAAG TTAGGCCTCT TCGCAGTTGA CATGTATGAC GACATTAAGT CTAAACTGAA CGAATGGGAT GAACGTAAGT ATGGCCTGAT CCTCGATGGT CTGCGCTACG AAGCTCATGA ACTGCTGAAA GAACCGGTCG TAGAAGTGGT GGACCCTTCC CGCATTGTTT CTGACGCCTC CGGTATCTGC ATCCTCGATC TGGGCCGTCT GAACGTACAG GACTTAGGCA AAGCACATGA ATTTCAGGTA GCTATTCACG ACGGTTGTAA ATGCTCTTCC CTGGCCCTTT ACTTTGACTG CATCTTTGAA CCGGTAGCTT CTGATAGCGC TAACGTGATC CAGTCCCTGA AAGGTAAACG TTTCCACACT CTCGAGTCCT ACCGCGAAGT TCTGGGTATC GACGAACTGA CTTCCTATGT GACCTCTCCG GACTACCCTC ACCCTGAAGA ACCAATGAGC ACCGCTGTTG TTATGTCTAC CCGTGCCGAC CAGCCTCCGA CCCATTGGAA GCAGGTTATC CTGCATCTGA CCGACCACAA CAACATGCCG GTTGCAATCC AGGGCGCTAT TGGCGGCAAG TTATCCCTGC TCAACAACTC TTCCAACTGC CGCTGGCTGG ATATTCTGTT TCAGCCTGAC GACACCTCCT AAAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for BaboA.01523.b.D16.PD00115
PURIFICATION DATe: 3/8/2010
CONCENTRATION: 15.17mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 33
PERCENT COVERAGE: 21
Protocol Notes
notes unavailable
Validated AA Sequence
MGHHHXXXXX AXXXXXXXXX XXAAAAXXXL XXXSRLRRPP RCXXXXXXXA KRXGKEMDSL RFLYDGIXIX AXXTPXXXXX XXXXXXXXXX EXXXGYFXG
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYFKG YKNLAIHREM VNDRVRTGAY ESFIRLNKSL FKDKVVLDVG SGCGILSLFA AKAGARLVVG IDNCDNMIKM SENHARINGI GNVQFLHGKV EDSDLVYSGG VVQFKRDDLP CEPFKCDILI SEWMGYALLY ENMLSSVLFA RDRYLISGGL MVPSKVKLGL FAVDMYDDIK SKLNEWDERK YGLILDGLRY EAHELLKEPV VEVVDPSRIV SDASGICILD LGRLNVQDLG KAHEFQVAIH DGCKCSSLAL YFDCIFEPVA SDSANVIQSL KGKRFHTLES YREVLGIDEL TSYVTSPDYP HPEEPMSTAV VMSTRADQPP THWKQVILHL TDHNNMPVAI QGAIGGKLSL LNNSSNCRWL DILFQPDDTS
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATA CTTCAAAGGC TACAAAAACT TAGCTATTCA TCGCGAAATG GTTAACGACC GCGTACGCAC CGGTGCGTAT GAATCTTTCA TTCGTCTCAA CAAGTCTCTG TTTAAAGATA AAGTAGTTCT GGACGTTGGT TCTGGTTGTG GTATTCTGTC TCTGTTCGCT GCTAAGGCAG GCGCTCGTCT GGTTGTCGGC ATTGACAACT GCGACAATAT GATTAAGATG AGCGAGAATC ACGCACGCAT CAACGGTATC GGCAACGTGC AGTTCTTGCA TGGTAAAGTT GAAGACTCTG ACCTGGTTTA CTCTGGCGGC GTAGTTCAAT TCAAACGTGA TGACCTGCCG TGCGAGCCGT TCAAATGCGA CATCCTGATC AGCGAGTGGA TGGGCTACGC TCTGCTCTAT GAAAATATGC TGAGCTCTGT GCTCTTCGCC CGCGATCGCT ACCTGATCAG CGGCGGTCTG ATGGTTCCAT CTAAAGTAAA GTTAGGCCTC TTCGCAGTTG ACATGTATGA CGACATTAAG TCTAAACTGA ACGAATGGGA TGAACGTAAG TATGGCCTGA TCCTCGATGG TCTGCGCTAC GAAGCTCATG AACTGCTGAA AGAACCGGTC GTAGAAGTGG TGGACCCTTC CCGCATTGTT TCTGACGCCT CCGGTATCTG CATCCTCGAT CTGGGCCGTC TGAACGTACA GGACTTAGGC AAAGCACATG AATTTCAGGT AGCTATTCAC GACGGTTGTA AATGCTCTTC CCTGGCCCTT TACTTTGACT GCATCTTTGA ACCGGTAGCT TCTGATAGCG CTAACGTGAT CCAGTCCCTG AAAGGTAAAC GTTTCCACAC TCTCGAGTCC TACCGCGAAG TTCTGGGTAT CGACGAACTG ACTTCCTATG TGACCTCTCC GGACTACCCT CACCCTGAAG AACCAATGAG CACCGCTGTT GTTATGTCTA CCCGTGCCGA CCAGCCTCCG ACCCATTGGA AGCAGGTTAT CCTGCATCTG ACCGACCACA ACAACATGCC GGTTGCAATC CAGGGCGCTA TTGGCGGCAA GTTATCCCTG CTCAACAACT CTTCCAACTG CCGCTGGCTG GATATTCTGT TTCAGCCTGA CGACACCTCC TAAAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T
Details for BaboA.01523.b.D13.PD00163
PURIFICATION DATe: 9/2/2010
CONCENTRATION: 22.44mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: data unavailable
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGLAIH REMVNDRVRT GAYESFIRLN KSLFKDKVVL DVGSGCGILS LFAAKAGARL VVGIDNCDNM IKMSENHARI NGIGNVQFLH GKVEDSDLVY SGGVVQFKRD DLPCEPFKCD ILISEWMGYA LLYENMLSSV LFARDRYLIS GGLMVPSKVK LGLFAVDMYD DIKSKLNEWD ERKYGLILDG LRYEAHELLK EPVVEVVDPS RIVSDASGIC ILDLGRLNVQ DLGKAHEFQV AIHDGCKCSS LALYFDCIFE PVASDSANVI QSLKGKRFHT LESYREVLGI DELTSYVTSP DYPHPEEPMS TAVVMSTRAD QPPTHWKQVI LHLTDHNNMP VAIQGAIGGK LSLLNNSSNC RWLDILFQPD DTSLLGECYY KLM
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATT AGCTATTCAT CGCGAAATGG TTAACGACCG CGTACGCACC GGTGCGTATG AATCTTTCAT TCGTCTCAAC AAGTCTCTGT TTAAAGATAA AGTAGTTCTG GACGTTGGTT CTGGTTGTGG TATTCTGTCT CTGTTCGCTG CTAAGGCAGG CGCTCGTCTG GTTGTCGGCA TTGACAACTG CGACAATATG ATTAAGATGA GCGAGAATCA CGCACGCATC AACGGTATCG GCAACGTGCA GTTCTTGCAT GGTAAAGTTG AAGACTCTGA CCTGGTTTAC TCTGGCGGCG TAGTTCAATT CAAACGTGAT GACCTGCCGT GCGAGCCGTT CAAATGCGAC ATCCTGATCA GCGAGTGGAT GGGCTACGCT CTGCTCTATG AAAATATGCT GAGCTCTGTG CTCTTCGCCC GCGATCGCTA CCTGATCAGC GGCGGTCTGA TGGTTCCATC TAAAGTAAAG TTAGGCCTCT TCGCAGTTGA CATGTATGAC GACATTAAGT CTAAACTGAA CGAATGGGAT GAACGTAAGT ATGGCCTGAT CCTCGATGGT CTGCGCTACG AAGCTCATGA ACTGCTGAAA GAACCGGTCG TAGAAGTGGT GGACCCTTCC CGCATTGTTT CTGACGCCTC CGGTATCTGC ATCCTCGATC TGGGCCGTCT GAACGTACAG GACTTAGGCA AAGCACATGA ATTTCAGGTA GCTATTCACG ACGGTTGTAA ATGCTCTTCC CTGGCCCTTT ACTTTGACTG CATCTTTGAA CCGGTAGCTT CTGATAGCGC TAACGTGATC CAGTCCCTGA AAGGTAAACG TTTCCACACT CTCGAGTCCT ACCGCGAAGT TCTGGGTATC GACGAACTGA CTTCCTATGT GACCTCTCCG GACTACCCTC ACCCTGAAGA ACCAATGAGC ACCGCTGTTG TTATGTCTAC CCGTGCCGAC CAGCCTCCGA CCCATTGGAA GCAGGTTATC CTGCATCTGA CCGACCACAA CAACATGCCG GTTGCAATCC AGGGCGCTAT TGGCGGCAAG TTATCCCTGC TCAACAACTC TTCCAACTGC CGCTGGCTGG ATATTCTGTT TCAGCCTGAC GACACCTCCC TGCTGGGTGA ATGCTACTAC AAACTGATGT AAAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT