SSGCID: Target Page

BaboA.01331.a

phosphoglycerate kinase

CENTER ID:	BaboA.01331.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.01331.a.A1.GE34111	full length		1	415

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_IV008070
RefSeq:	XP_001610729.1
UniProt:	A7ARJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MESMLSSKLG LADIVDKLPG ARILLRADFN VPIKEGVIGD CTRIKATIPT IEFLLKHNVK SIVMMSHLGR PDGSRVEKYS LRPVATELSK ILNKKVDFLD DCVGETVEKF CQEAPTGTIV LLENLRFHAA EEGVKDKVKV DDSIIQAFRN SLTKLGDIYV NDAFGTAHRA HSSMVGVNVP IKVAGLLMKK ELDYFAKILE TPKRPFMSIL GGAKVKDKIQ LIKSIMRKVD ILFIGGGMAY TFKNVLNGMP IGQSLYDEEG AKIVPDIMAE AKQLGVKVYL PIDFKAATAF SNDAPFKVVS EADGISDDSQ GLDCGPKTVE LLKTVIEGCQ TIVWNGPLGV FEFSNFATGS IAALDIVGEA TKRGAISVIG GGDTASLAEQ TGRAHLFSHV STGGGASLEL MEGKVLPGVD ALSSK

NT Sequence

atggaatcca tgctctcgtc taaactcggc cttgccgata ttgtcgataa gttgcctggt gctagaatat tgctaagggc cgactttaat gtgcccatta aagaaggagt catcggggat tgcacacgca taaaagcaac aattcctacg atcgaatttc ttctaaaaca caatgtcaaa tctattgtta tgatgtcgca ccttggaagg cctgatggta gccgtgtaga aaagtactct cttcgtccgg tcgctaccga gctctccaaa attttaaaca aaaaggttga tttccttgat gactgcgttg gtgaaactgt tgaaaagttt tgtcaggaag ctcctacagg aacgatcgtg ttgttggaaa atcttagatt ccatgctgca gaagagggag tgaaagataa ggtaaaggtc gatgacagca ttatccaggc attccgcaat agcctgacca aattaggtga tatttatgtt aatgatgcat ttggaacagc tcatagagct cacagttcta tggtcggtgt aaatgtgcct attaaagtgg caggactcct tatgaaaaag gagctagact actttgccaa gatacttgaa actcctaaga gaccgttcat gagtattttg ggcggtgcta aagttaagga caaaatacaa ctaattaaat ccataatgcg aaaggttgat attctattca taggcggtgg tatggcctat acgttcaaga atgttctcaa tggaatgccc ataggtcaat cattatatga tgaagaagga gcgaaaattg ttccagatat aatggctgaa gcgaagcaac ttggtgttaa agtatacctt ccaattgact ttaaagctgc tactgcgttt tccaatgacg ctccttttaa ggttgttagc gaagcagatg ggatatctga tgattcacaa ggtttggact gtggcccaaa gacagtggaa ctgctcaaaa ctgttataga aggctgccaa accattgtgt ggaacggccc acttggtgtc ttcgaatttt ctaattttgc aacgggttcg attgccgcat tagatatcgt tggagaagct accaaaagag gagcaatcag tgtgattggt ggcggtgata cagcatctct tgccgaacaa acaggcaggg ctcatctttt cagccacgtt tctaccggtg gtggcgcctc actcgaactg atggaaggaa aggttcttcc aggtgtcgat gccctttcaa gcaagtga

Details for BaboA.01331.a.A1.GE34111

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ttgcttgaaa gggcatcgac acctggaaga acctttcctt ccatcagttc gagtgaggcg ccaccaccgg tagaaacgtg gctgaaaaga tgagccctgc ctgtttgttc ggcaagagat gctgtatcac cgccaccaat cacactgatt gctcctcttt tggtagcttc tccaacgata tctaatgcgg caatcgaacc cgttgcaaaa ttagaaaatt cgaagacacc aagtgggccg ttccacacaa tggtttggca gccttctata acagttttga gcagttccac tgtctttggg ccacagtcca aaccttgtga atcatcagat atcccatctg cttcgctaac aaccttaaaa ggagcgtcat tggaaaacgc agtagcagct ttaaagtcaa ttggaaggta tactttaaca ccaagttgct tcgcttcagc cattatatct ggaacaattt tcgctccttc ttcatcatat aatgattgac ctatgggcat tccattgaga acattcttga acgtataggc cataccaccg cctatgaata gaatatcaac ctttcgcatt atggatttaa ttagttgtat tttgtcctta actttagcac cgcccaaaat actcatgaac ggtctcttag gagtttcaag tatcttggca aagtagtcta gctccttttt cataaggagt cctgccactt taataggcac atttacaccg accatagaac tgtgagctct atgagctgtt ccaaatgcat cattaacata aatatcacct aatttggtca ggctattgcg gaatgcctgg ataatgctgt catcgacctt taccttatct ttcactccct cttctgcagc atggaatcta agattttcca acaacacgat cgttcctgta ggagcttcct gacaaaactt ttcaacagtt tcaccaacgc agtcatcaag gaaatcaacc tttttgttta aaattttgga gagctcggta gcgaccggac gaagagagta cttttctaca cggctaccat caggccttcc aaggtgcgac atcataacaa tagatttgac attgtgtttt agaagaaatt cgatcgtagg aattgttgct tttatgcgtg tgcaatcccc gatgactcct tctttaatgg gcacattaaa gtcggccctt agcaatattc tagcaccagg caacttatcg acaatatcgg caaggccgag tttagacgag agcatggatt ccatcgaacc aggaccctgg gtctgagctt ccaggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMESMLSSKL GLADIVDKLP GARILLRADF NVPIKEGVIG DCTRIKATIP TIEFLLKHNV KSIVMMSHLG RPDGSRVEKY SLRPVATELS KILNKKVDFL DDCVGETVEK FCQEAPTGTI VLLENLRFHA AEEGVKDKVK VDDSIIQAFR NSLTKLGDIY VNDAFGTAHR AHSSMVGVNV PIKVAGLLMK KELDYFAKIL ETPKRPFMSI LGGAKVKDKI QLIKSIMRKV DILFIGGGMA YTFKNVLNGM PIGQSLYDEE GAKIVPDIMA EAKQLGVKVY LPIDFKAATA FSNDAPFKVV SEADGISDDS QGLDCGPKTV ELLKTVIEGC QTIVWNGPLG VFEFSNFATG SIAALDIVGE ATKRGAISVI GGGDTASLAE QTGRAHLFSH VSTGGGASLE LMEGKVLPGV DALSSK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatccatg ctctcgtcta aactcggcct tgccgatatt gtcgataagt tgcctggtgc tagaatattg ctaagggccg actttaatgt gcccattaaa gaaggagtca tcggggattg cacacgcata aaagcaacaa ttcctacgat cgaatttctt ctaaaacaca atgtcaaatc tattgttatg atgtcgcacc ttggaaggcc tgatggtagc cgtgtagaaa agtactctct tcgtccggtc gctaccgagc tctccaaaat tttaaacaaa aaggttgatt tccttgatga ctgcgttggt gaaactgttg aaaagttttg tcaggaagct cctacaggaa cgatcgtgtt gttggaaaat cttagattcc atgctgcaga agagggagtg aaagataagg taaaggtcga tgacagcatt atccaggcat tccgcaatag cctgaccaaa ttaggtgata tttatgttaa tgatgcattt ggaacagctc atagagctca cagttctatg gtcggtgtaa atgtgcctat taaagtggca ggactcctta tgaaaaagga gctagactac tttgccaaga tacttgaaac tcctaagaga ccgttcatga gtattttggg cggtgctaaa gttaaggaca aaatacaact aattaaatcc ataatgcgaa aggttgatat tctattcata ggcggtggta tggcctatac gttcaagaat gttctcaatg gaatgcccat aggtcaatca ttatatgatg aagaaggagc gaaaattgtt ccagatataa tggctgaagc gaagcaactt ggtgttaaag tataccttcc aattgacttt aaagctgcta ctgcgttttc caatgacgct ccttttaagg ttgttagcga agcagatggg atatctgatg attcacaagg tttggactgt ggcccaaaga cagtggaact gctcaaaact gttatagaag gctgccaaac cattgtgtgg aacggcccac ttggtgtctt cgaattttct aattttgcaa cgggttcgat tgccgcatta gatatcgttg gagaagctac caaaagagga gcaatcagtg tgattggtgg cggtgataca gcatctcttg ccgaacaaac aggcagggct catcttttca gccacgtttc taccggtggt ggcgcctcac tcgaactgat ggaaggaaag gttcttccag gtgtcgatgc cctttcaagc aagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa