SSGCID: Target Page

BaboA.01278.a

prolyl-t-RNA synthase, putative

CENTER ID:	BaboA.01278.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.01278.a.A1.GE34112	full length		1	439

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_III011010
RefSeq:	XP_001612221.1
UniProt:	A7AQ19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGFRVYKAGI PKLVTRSHAD YNWDVSHDNE LFGVAESRGD VSGNRNNLRK PGILTHMSPG ISTVLPIGKR IVNRIERVIH VEMSRIGAKE VELPMLLSSD TINERRNEFG RELFSLTDRN GKHYDLCPTC EELICRMLDK ALSPLSKRNL PIYLYQIGRK FRDEARPCNS NMRCREFIMK DAYSFHSDKD SAAMDYNKFK QAYERIFTLL ELSFDTVTIG DTEHEFRAML PNKKTLMEIA HIFQLGDAIT TEAGLKYEGE GRMKHPVYLN SYGIGIHRLL QAAASQHADS EGIRLPQIIA PYDVAVIPCD ENSEQASADI YTLLSKRGID TFLDTRKAPI NQRVSDMMNI GIPHIVYVSS VYTEKAMSCP NEDITMKIIE GSKKSNYYHQ YQSPTYSLLQ DKEIPDTIQR INVGYSHRSF PGEYTIPVSK LLQLLAVAR

NT Sequence

atgggattca gggtatataa agcaggtata ccgaaactgg ttacaagaag ccatgccgat tacaattggg atgtatcgca tgacaatgag ttgtttggtg tcgctgaatc acgaggcgac gtctctggta atcgcaacaa ccttcgtaaa ccgggcatcc taactcatat gtcacctgga atcagtactg tactgcccat aggtaaacgg attgttaacc gtatcgaacg tgtaattcac gttgagatgt ccagaatcgg tgcaaaggaa gttgaattgc caatgttgct atcaagcgat actattaatg aacggcgtaa tgaattcggt agggagttgt tttcgttgac tgacagaaac ggtaaacatt atgacttgtg tccaacatgt gaagaactaa tttgtaggat gctggataaa gcgttatccc ccttgtctaa acggaatcta cctatatatc tataccaaat agggcgtaaa tttcgcgatg aggctagacc atgcaactcc aatatgcgat gtcgtgaatt tataatgaag gatgcatata gttttcacag tgacaaggac tccgcagcta tggattataa caaattcaag caggcttacg aacgcatttt cactttactg gagttgtctt ttgacaccgt gactattggg gatacagaac acgaattcag ggcaatgctc ccaaataaaa aaacacttat ggagattgca catatattcc agttaggcga tgctataact acagaagccg gattgaaata tgaaggtgaa ggtagaatga agcacccggt gtacctcaat tcatacggca tcggaatcca tagactcctt caggcggcag cttcacaaca tgctgacagt gaaggcataa ggttaccaca gataattgcg ccttacgacg ttgcagttat cccgtgcgac gaaaattcag agcaagcttc agccgatatc tacacgctgc tctccaagcg aggtattgac acatttcttg atactcgcaa agcgcctata aaccagcgtg tatcggatat gatgaacatc ggcatccctc atatcgtata tgtatcgtct gtatacaccg aaaaagcaat gtcgtgccct aatgaagata taacgatgaa aataatagaa gggtcaaaga aatccaatta ttaccatcaa tatcaatctc caacctatag tcttttgcaa gataaggaaa taccggatac aatccagcgg ataaatgtcg gatactccca tagatcattc ccaggtgagt acacaatacc tgtttccaaa ctgttgcaac tgcttgctgt agccaggtaa

Details for BaboA.01278.a.A1.GE34112

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	52kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

ctacagcaag cagttgcaac agtttggaaa caggtattgt gtactcacct gggaatgatc tatgggagta tccgacattt atccgctgga ttgtatccgg tatttcctta tcttgcaaaa gactataggt tggagattga tattgatggt aataattgga tttctttgac ccttctatta ttttcatcgt tatatcttca ttagggcacg acattgcttt ttcggtgtat acagacgata catatacgat atgagggatg ccgatgttca tcatatccga tacacgctgg tttataggcg ctttgcgagt atcaagaaat gtgtcaatac ctcgcttgga gagcagcgtg tagatatcgg ctgaagcttg ctctgaattt tcgtcgcacg ggataactgc aacgtcgtaa ggcgcaatta tctgtggtaa ccttatgcct tcactgtcag catgttgtga agctgccgcc tgaaggagtc tatggattcc gatgccgtat gaattgaggt acaccgggtg cttcattcta ccttcacctt catatttcaa tccggcttct gtagttatag catcgcctaa ctggaatata tgtgcaatct ccataagtgt ttttttattt gggagcattg ccctgaattc gtgttctgta tccccaatag tcacggtgtc aaaagacaac tccagtaaag tgaaaatgcg ttcgtaagcc tgcttgaatt tgttataatc catagctgcg gagtccttgt cactgtgaaa actatatgca tccttcatta taaattcacg acatcgcata ttggagttgc atggtctagc ctcatcgcga aatttacgcc ctatttggta tagatatata ggtagattcc gtttagacaa gggggataac gctttatcca gcatcctaca aattagttct tcacatgttg gacacaagtc ataatgttta ccgtttctgt cagtcaacga aaacaactcc ctaccgaatt cattacgccg ttcattaata gtatcgcttg atagcaacat tggcaattca acttcctttg caccgattct ggacatctca acgtgaatta cacgttcgat acggttaaca atccgtttac ctatgggcag tacagtactg attccaggtg acatatgagt taggatgccc ggtttacgaa ggttgttgcg attaccagag acgtcgcctc gtgattcagc gacaccaaac aactcattgt catgcgatac atcccaattg taatcggcat ggcttcttgt aaccagtttc ggtatacctg ctttatatac cctgaatccc atcgaaccag gaccctgggt ctgagcttcc agggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGFRVYKAG IPKLVTRSHA DYNWDVSHDN ELFGVAESRG DVSGNRNNLR KPGILTHMSP GISTVLPIGK RIVNRIERVI HVEMSRIGAK EVELPMLLSS DTINERRNEF GRELFSLTDR NGKHYDLCPT CEELICRMLD KALSPLSKRN LPIYLYQIGR KFRDEARPCN SNMRCREFIM KDAYSFHSDK DSAAMDYNKF KQAYERIFTL LELSFDTVTI GDTEHEFRAM LPNKKTLMEI AHIFQLGDAI TTEAGLKYEG EGRMKHPVYL NSYGIGIHRL LQAAASQHAD SEGIRLPQII APYDVAVIPC DENSEQASAD IYTLLSKRGI DTFLDTRKAP INQRVSDMMN IGIPHIVYVS SVYTEKAMSC PNEDITMKII EGSKKSNYYH QYQSPTYSLL QDKEIPDTIQ RINVGYSHRS FPGEYTIPVS KLLQLLAVAR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggattcagg gtatataaag caggtatacc gaaactggtt acaagaagcc atgccgatta caattgggat gtatcgcatg acaatgagtt gtttggtgtc gctgaatcac gaggcgacgt ctctggtaat cgcaacaacc ttcgtaaacc gggcatccta actcatatgt cacctggaat cagtactgta ctgcccatag gtaaacggat tgttaaccgt atcgaacgtg taattcacgt tgagatgtcc agaatcggtg caaaggaagt tgaattgcca atgttgctat caagcgatac tattaatgaa cggcgtaatg aattcggtag ggagttgttt tcgttgactg acagaaacgg taaacattat gacttgtgtc caacatgtga agaactaatt tgtaggatgc tggataaagc gttatccccc ttgtctaaac ggaatctacc tatatatcta taccaaatag ggcgtaaatt tcgcgatgag gctagaccat gcaactccaa tatgcgatgt cgtgaattta taatgaagga tgcatatagt tttcacagtg acaaggactc cgcagctatg gattataaca aattcaagca ggcttacgaa cgcattttca ctttactgga gttgtctttt gacaccgtga ctattgggga tacagaacac gaattcaggg caatgctccc aaataaaaaa acacttatgg agattgcaca tatattccag ttaggcgatg ctataactac agaagccgga ttgaaatatg aaggtgaagg tagaatgaag cacccggtgt acctcaattc atacggcatc ggaatccata gactccttca ggcggcagct tcacaacatg ctgacagtga aggcataagg ttaccacaga taattgcgcc ttacgacgtt gcagttatcc cgtgcgacga aaattcagag caagcttcag ccgatatcta cacgctgctc tccaagcgag gtattgacac atttcttgat actcgcaaag cgcctataaa ccagcgtgta tcggatatga tgaacatcgg catccctcat atcgtatatg tatcgtctgt atacaccgaa aaagcaatgt cgtgccctaa tgaagatata acgatgaaaa taatagaagg gtcaaagaaa tccaattatt accatcaata tcaatctcca acctatagtc ttttgcaaga taaggaaata ccggatacaa tccagcggat aaatgtcgga tactcccata gatcattccc aggtgagtac acaatacctg tttccaaact gttgcaactg cttgctgtag ccaggtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa