BaboA.01191.a: Dihydrofolate reductase/thymidylate synthase
Target Characteristics
from organism:
Babesia bovis T2Bo
most recent status:
in PDB
center reference id:
BaboA.01191.a
is community request:
True
associated disease:
Babesiosis (atypical)
NIH risk group:
2
is select agent:
False
NIH priority
I
Ordering Clones & Proteins
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Clones*
CENTER
DOMAIN/REGION
INFO
AA
AA
ORDER
BaboA.01191.a.D11.GE00003
BaboA.01191.a
more 1
511
GE00003
BaboA.01191.a.D12.GE00001
BaboA.01191.a
more 1
188
GE00001
BaboA.01191.a.D13.GE00001
BaboA.01191.a
more 203
511
GE00001
BaboA.01191.a.D14.GE00001
BaboA.01191.a
more 203
499
GE00001
BaboA.01191.a.D15.GE00001
BaboA.01191.a
more 225
511
GE00001
BaboA.01191.a.D16.GE00001
BaboA.01191.a
more 225
499
GE00001
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER
DOMAIN/REGION
INFO
AA
AA
ORDER
BaboA.01191.a.D13.PD00068
BaboA.01191.a
more 203
511
PD00068
Structures
3I3R
Deposited: 6/30/2009
Determination: XRayDiffraction Image
3K2H
Deposited: 9/30/2009
Determination: XRayDiffraction Image
3KJR
Deposited: 11/3/2009
Determination: XRayDiffraction Image
3NRR
Deposited: 6/30/2010
Determination: XRayDiffraction Image
Publications by SSGCID
Inhibitor-bound complexes of dihydrofolate reductase-thymidylate synthase from Babesia bovis
Abendroth J, Begley DW, Edwards TE, Hartley RC, Lorimer DD, Myler PJ, Raymond AC, Sankaran B, Smith ER, Staker BL, Stewart LJ
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1070-7
External Resources Enzyme & Pathway Information
Pathway
Pathway ID
EC Number
N10-formyl-tetrahydrofolate biosynthesis
1CMET2-PWY 1.5.1.3
superpathway of chorismate metabolism
ALL-CHORISMATE-PWY 1.5.1.3
superpathway of tetrahydrofolate biosynthesis and salvage
FOLSYN-PWY 1.5.1.3
folate transformations II
PWY-3841 1.5.1.3
superpathway of tetrahydrofolate biosynthesis
PWY-6612 1.5.1.3
tetrahydrofolate biosynthesis
PWY-6614 1.5.1.3
N10-formyl-tetrahydrofolate biosynthesis
1CMET2-PWY 2.1.1.45
purine and pyrimidine metabolism
P1-PWY 2.1.1.45
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)
PWY0-166 2.1.1.45
folate transformations II
PWY-3841 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis I
PWY-7184 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis II
PWY-7187 2.1.1.45
pyrimidine deoxyribonucleotides de novo biosynthesis IV
PWY-7198 2.1.1.45
pyrimidine deoxyribonucleosides salvage
PWY-7199 2.1.1.45
superpathway of pyrimidine deoxyribonucleoside salvage
PWY-7200 2.1.1.45
pyrimidine deoxyribonucleotides biosynthesis from CTP
PWY-7210 2.1.1.45
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PWY-7211 2.1.1.45
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MSNSYEGCGD LTIFVAVALN KVIGHKNQIP WPHITHDFRF LRNGTTYIPP EVLSKNPDIQ NVVIFGRKTY ESIPKASLPL KNRINVILSR TVKEVPGCLV YEDLSTAIRD LRANVPHNKI FILGGSFLYK EVLDNGLCDK IYLTRLNKEY PGDTYFPDIP DTFEITAISP TFSTDFVSYD FVIYERKDCK TVFPDPPFDQ LLMTGTDISV PKPKYVACPG VRIRNHEEFQ YLDILADVLS HGVLKPNRTG TDAYSKFGYQ MRFDLSRSFP LLTTKKVALR SIIEELLWFI KGSTNGNDLL AKNVRIWELN GRRDFLDKNG FTDREEHDLG PIYGFQWRHF GAEYLDMHAD YTGKGIDQLA EIINRIKTNP NDRRLIVCSW NVSDLKKMAL PPCHCFFQFY VSDNKLSCMM HQRSCDLGLG VPFNIASYSI LTAMVAQVCG LGLGEFVHNL ADAHIYVDHV DAVTTQIARI PHPFPRLRLN PDIRNIEDFT IDDIVVEDYV SHPPIPMAMS A
NT Sequence
atgtctaatt catacgaagg gtgtggagat cttacgattt tcgtagccgt tgccttgaat aaggtaattg gccataaaaa ccaaattccc tggccgcata ttacgcatga ttttcgtttt ttgcgtaatg gcacaactta cattccacct gaggtgttgt ctaagaaccc agatatacag aatgttgtga ttttcggtag gaagacttat gaaagtatcc ctaaagcttc gctccctctt aaaaaccgta taaacgtgat actgtctcgt actgtcaagg aagttcctgg ttgtttggtg tacgaggacc tttctactgc cattcgtgat ttgcgtgcaa atgtccctca taacaagatt tttatcttag gcggttcttt tctttacaag gaggtgcttg acaatggttt gtgtgataag atttatttga ctcgtctcaa caaggagtat cctggtgaca cctacttccc tgatattccg gatacttttg agataaccgc cattagtcct acgttttcca ctgactttgt cagttatgat tttgttattt acgagcgtaa ggattgcaag actgttttcc ccgatcctcc ttttgatcaa ttattgatga cagggactga cattagcgtt cccaaaccaa agtacgttgc ttgtcctggc gtcaggatac gcaaccatga agagttccaa taccttgaca tattggcaga tgtgctaagt catggtgtat tgaagcccaa ccgtaccggt actgatgcct actctaagtt tggctatcag atgcgatttg acctttcacg ttcattcccg ttattgacta ccaagaaggt agcattgcgc agcataattg aggaactgct ctggtttatt aagggtagta ccaacggcaa cgacctactg gctaagaatg tgcgcatttg ggagcttaac ggtcgtcgtg atttccttga taagaatggt tttacagatc gtgaagagca tgatttaggt cctatatatg gtttccaatg gcgtcatttt ggtgctgagt acttggatat gcacgctgat tacactggta agggtattga tcagcttgct gagataatta accgcattaa aacgaaccca aacgaccgta ggctgattgt atgctcttgg aatgtttcgg acctaaagaa gatggctttg cctccttgtc attgtttctt ccaattttat gtcagtgaca acaagctttc ttgtatgatg catcagcgtt cctgtgacct tggtttgggt gtacctttca acattgcttc ttatagtata cttactgcta tggtggctca ggtttgcggt ttaggtttag gggagtttgt ccataatttg gcagacgccc acatttatgt tgaccacgtg gacgccgtta ctactcagat tgctcgcatt cctcatccat ttccccgttt gcgtttgaac cctgatatta ggaatatcga ggactttact attgacgaca ttgtagttga ggattacgtc tcgcatcctc ccatacccat ggccatgtcc gcatga
harvested on: data unavailable
sequenced on: 7/28/2009
expected MW: 0kDa
observed MW: 0kDa
MW ratio: data unavailable
antibiotic marker:
kanamycin
expression colonies count:
data unavailable
total expression level:
Moderate Expression
soluble expression level:
Moderate Expression
expression host:
data unavailable
sequencing result:
pass with incomplete coverage
percent identity: 81
percent coverage: 63
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMSNS YEGCGDLTIF VAVALNKVIG HKNQIPWPHI THDFRFLRNG TTYIPPEVLS KNPDIQNVVI FGRKTYESIP KASLPLKNRI NVILSRTVKE VPGCLVYEDL STAIRDLRAN VPHNKIFILG GSFLYKEVLD NGLCDKIYLT RLNKEYPGDT YFPDIPDTFE ITAISPTFST DFVSYDFVIY ERKDCKTVFP DPPFXXLLMT GTDISVPKPK YVXXPXFVXV TXKNXXIXTS XLXXCPXXCX XXXXXXXLXX XXXXXXXXXX XLXXXXXXXX XXXXXXXXXX XXXXXX
CTNNNNNNAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGAATG TCCAATAGCT ATGAAGGTTG TGGCGATCTG ACTATCTTCG TAGCTGTCGC ACTCAACAAA GTTATCGGTC ATAAAAATCA GATCCCTTGG CCACACATAA CCCATGACTT TCGTTTCCTG CGTAACGGCA CCACCTACAT CCCGCCGGAA GTACTGAGCA AGAATCCTGA TATCCAGAAC GTGGTGATCT TCGGCCGTAA AACTTACGAG TCTATCCCTA AGGCCTCGCT GCCGCTCAAG AATCGTATCA ACGTAATCCT CTCTCGTACC GTTAAGGAAG TGCCTGGTTG CTTAGTTTAT GAAGATCTGT CCACTGCGAT TCGCGACCTG CGTGCAAACG TACCTCACAA CAAGATCTTC ATCCTGGGCG GTTCCTTCCT CTACAAAGAA GTTCTTGACA ACGGTCTGTG TGACAAGATC TATCTGACCC GCCTCAACAA GGAATACCCG GGCGACACTT ACTTCCCGGA TATCCCTGAT ACCTTCGAAA TAACCGCCAT CTCTCCTACC TTCTCCACTG ACTTCGTTTC TTACGATTTC GTAATTTACG AGCGTAAAGA TTGCAAGACT GTGTTCCCGG ACCCGCCATT TGANCNACTC CTGATGACCG GCACCGACAT CTCTGTTCCT AAACCGAAGT ACGTANCCTG NCCGGNGTTC GTATNCGTAA CCNCNAAGAA TNNNAGNATC NTGACNTCTN AGCTGANNNG CTGTCCNNNG NNGTGCTNAN NCNANCNNCN NCNGNNNNNA CTNNNNNNAA NTTNNNNNCN NNNGNNNNNN NNNNNNCNNN ANCTNCNNCN CNNNCNNNAN ANNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNCNNNNNN NNNNNNNNNN NNNNNNAA
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMSNS YEGCGDLTIF VAVALNKVIG HKNQIPWPHI THDFRFLRNG TTYIPPEVLS KNPDIQNVVI FGRKTYESIP KASLPLKNRI NVILSRTVKE VPGCLVYEDL STAIRDLRAN VPHNKIFILG GSFLYKEVLD NGLCDKIYLT RLNKEYPGDT YFPDIPDTFE ITAISPTFST DFVSYDFVIY ERKDCKTVFP DPPFDQLLMT GTDISVPKPK YVACPGVRIR NHEEFQYLDI LADVLSHGVL KPNRTGTDAY SKFGYQMRFD LSRSFPLLTT KKVALRSIIE ELLWFIKGST NGNDLLAKNV RIWELNGRRD FLDKNGFTDR EEHDLGPIYG FQWRHFGAEY LDMHADYTGK GIDQLAEIIN RIKTNPNDRR LIVCSWNVSD LKKMALPPCH CFFQFYVSDN KLSCMMHQRS CDLGLGVPFN IASYSILTAM VAQVCGLGLG EFVHNLADAH IYVDHVDAVT TQIARIPHPF PRLRLNPDIR NIEDFTIDDI VVEDYVSHPP IPMAMSA
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GTCCAATAGC TATGAAGGTT GTGGCGATCT GACTATCTTC GTAGCTGTCG CACTCAACAA AGTTATCGGT CATAAAAATC AGATCCCTTG GCCACACATA ACCCATGACT TTCGTTTCCT GCGTAACGGC ACCACCTACA TCCCGCCGGA AGTACTGAGC AAGAATCCTG ATATCCAGAA CGTGGTGATC TTCGGCCGTA AAACTTACGA GTCTATCCCT AAGGCCTCGC TGCCGCTCAA GAATCGTATC AACGTAATCC TCTCTCGTAC CGTTAAGGAA GTGCCTGGTT GCTTAGTTTA TGAAGATCTG TCCACTGCGA TTCGCGACCT GCGTGCAAAC GTACCTCACA ACAAGATCTT CATCCTGGGC GGTTCCTTCC TCTACAAAGA AGTTCTTGAC AACGGTCTGT GTGACAAGAT CTATCTGACC CGCCTCAACA AGGAATACCC GGGCGACACT TACTTCCCGG ATATCCCTGA TACCTTCGAA ATAACCGCCA TCTCTCCTAC CTTCTCCACT GACTTCGTTT CTTACGATTT CGTAATTTAC GAGCGTAAAG ATTGCAAGAC TGTGTTCCCG GACCCGCCAT TTGACCAACT CCTGATGACC GGCACCGACA TCTCTGTTCC TAAACCGAAG TACGTAGCCT GCCCTGGCGT TCGTATCCGT AACCACGAAG AATTCCAGTA TCTTGACATC TTAGCTGACG TGCTGTCCCA CGGTGTGCTG AAGCCGAACC GCACCGGCAC TGACGCTTAC TCGAAATTTG GTTACCAGAT GCGCTTCGAC CTTTCCCGTA GCTTCCCGCT CCTGACCACT AAGAAGGTTG CGCTCCGCTC TATCATCGAG GAACTGCTCT GGTTTATCAA AGGTTCTACC AACGGTAACG ATCTGTTAGC TAAGAATGTT CGTATCTGGG AACTGAACGG TCGTCGTGAC TTTCTTGACA AGAATGGTTT CACTGATCGC GAAGAGCACG ACCTGGGTCC GATTTACGGC TTCCAGTGGC GTCACTTTGG TGCTGAATAC CTTGACATGC ACGCTGATTA CACTGGCAAA GGTATCGATC AGTTAGCTGA AATCATTAAT CGCATCAAGA CTAACCCGAA CGATCGCCGT CTGATCGTTT GCTCCTGGAA CGTCTCTGAT CTCAAGAAAA TGGCACTGCC GCCTTGTCAC TGCTTCTTCC AGTTCTATGT TAGCGACAAC AAACTGAGCT GCATGATGCA CCAGCGTAGC TGCGATCTGG GCCTGGGCGT TCCGTTCAAT ATCGCTTCCT ACTCCATTCT GACCGCTATG GTTGCCCAAG TTTGTGGTCT TGGTCTGGGT GAATTCGTTC ATAACCTGGC TGATGCCCAC ATTTACGTGG ACCACGTTGA CGCTGTGACC ACCCAGATAG CTCGTATTCC TCACCCATTT CCGCGCCTGC GTCTTAATCC AGACATTCGT AACATTGAAG ACTTCACCAT CGACGATATC GTCGTGGAAG ATTACGTGTC CCACCCACCG ATTCCTATGG CCATGTCTGC ATAAAAGCTT GCGGCCGCAC TCGAGCACCA CCACCACCAC CACTGAGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG AT
purified on: 6/26/2009
concentration: 27.2mg/ml
observed molecular weight:
data unavailable
expression level:
High Expression
protein purification buffer:
20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
expression host:
data unavailable
vial count (approx.):
1
vial volume:
50µl
percent identity: 100
percent coverage: 61
MRFDLSRSFP LLTTKKVALR SIIEELLWFI KGSTNGNDLL AKNVRIWELN GRRDFLDKNG FTDREEHDLG PIYGFQWRHF GAEYLDMHAD YTGKGIDQLA EIINRIKTNP NDRRLIVCSW NVSDLKKMAL PPCHCFFQFY VSDNKLSCMM HQRSCDLGLG VPFNIASYSI LTAMVAQVCG LGLGEFVHNL ADAHIYVDHV DAVTTQIARI PHPFPRLRLN PDIRNIEDFT IDDIVVEDYV SHPPIPMAMS A
TTCNGGCTTT GTTAGCAGCC GGNTCTCNGT GGTGGTGGTG GTGGTGCTCG AGTGCGGCCG CAAGCTTTTA TGCAGACATG GCCATAGGAA TCGGTGGGTG GGACACGTAA TCTTCCACGA CGATATCGTC GATGGTGAAG TCTTCAATGT TACGAATGTC TGGATTAAGA CGCAGGCGCG GAAATGGGTG AGGAATACGA GCTATCTGGG TGGTCACAGC GTCAACGTGG TCCACGTAAA TGTGGGCATC AGCCAGGTTA TGAACGAATT CACCCAGACC AAGACCACAA ACTTGGGCAA CCATAGCGGT CAGAATGGAG TAGGAAGCGA TATTGAACGG AACGCCCAGG CCCAGATCGC AGCTACGCTG GTGCATCATG CAGCTCAGTT TGTTGTCGCT AACATAGAAC TGGAAGAAGC AGTGACAAGG CGGCAGTGCC ATTTTCTTGA GATCAGAGAC GTTCCAGGAG CAAACGATCA GACGGCGATC GTTCGGGTTA GTCTTGATGC GATTAATGAT TTCAGCTAAC TGATCGATAC CTTTGCCAGT GTAATCAGCG TGCATGTCAA GGTATTCAGC ACCAAAGTGA CGCCACTGGA AGCCGTAAAT CGGACCCAGG TCGTGCTCTT CGCGATCAGT GAAACCATTC TTGTCAAGAA AGTCACGACG ACCGTTCAGT TCCCAGATAC GAACATTCTT AGCTAACAGA TCGTTACCGT TGGTAGAACC TTTGATAAAC CAGAGCAGTT CCTCGATGAT AGAGCGGAGC GCAACCTTCT TAGTGGTCAG GAGCGGGAAG CTACGGGAAA GGTCGAAGCG CATCTGGTAA CCAAATTTCG AGTAAGCGTC AGTGCCNGTG CGGTTCGGCT TCAGCACACC GTGGGACAGC ACGTCAGCTA AGATGTCNAG ATACTGGAAT TCTTCGTGGT TNNNGATACG AACGCCNGGN NGGCTACGTA CTTCGGTTTN NNAACAGANN NGTCGGTGNC GNTCATTCCA CCGATTNNNN CANGNNNNNC NTCGANNATG TCNTNNTCNT NNNTGTNCAG ATNNTNNNNN NNNNGNCTNN CNNANTANGN NNNCCNNNNN NNAAAGCNNN NNNNTNNNNT TNNNNNNNCN NNNNNTNNNN NANNGNNNCN NNNGNNNNNN NNNNNGNNNN NNNNNNNNNN NNANANCNNN NNNNNNNNNN GNNNNAANNN NNNNNNNCCG G
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMTGT DISVPKPKYV ACPGVRIRNH EEFQYLDILA DVLSHGVLKP NRTGTDAYSK FGYQMRFDLS RSFPLLTTKK VALRSIIEEL LWFIKGSTNG NDLLAKNVRI WELNGRRDFL DKNGFTDREE HDLGPIYGFQ WRHFGAEYLD MHADYTGKGI DQLAEIINRI KTNPNDRRLI VCSWNVSDLK KMALPPCHCF FQFYVSDNKL SCMMHQRSCD LGLGVPFNIA SYSILTAMVA QVCGLGLGEF VHNLADAHIY VDHVDAVTTQ IARIPHPFPR LRLNPDIRNI EDFTIDDIVV EDYVSHPPIP MAMSA
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GACCGGCACC GACATCTCTG TTCCTAAACC GAAGTACGTA GCCTGCCCTG GCGTTCGTAT CCGTAACCAC GAAGAATTCC AGTATCTTGA CATCTTAGCT GACGTGCTGT CCCACGGTGT GCTGAAGCCG AACCGCACCG GCACTGACGC TTACTCGAAA TTTGGTTACC AGATGCGCTT CGACCTTTCC CGTAGCTTCC CGCTCCTGAC CACTAAGAAG GTTGCGCTCC GCTCTATCAT CGAGGAACTG CTCTGGTTTA TCAAAGGTTC TACCAACGGT AACGATCTGT TAGCTAAGAA TGTTCGTATC TGGGAACTGA ACGGTCGTCG TGACTTTCTT GACAAGAATG GTTTCACTGA TCGCGAAGAG CACGACCTGG GTCCGATTTA CGGCTTCCAG TGGCGTCACT TTGGTGCTGA ATACCTTGAC ATGCACGCTG ATTACACTGG CAAAGGTATC GATCAGTTAG CTGAAATCAT TAATCGCATC AAGACTAACC CGAACGATCG CCGTCTGATC GTTTGCTCCT GGAACGTCTC TGATCTCAAG AAAATGGCAC TGCCGCCTTG TCACTGCTTC TTCCAGTTCT ATGTTAGCGA CAACAAACTG AGCTGCATGA TGCACCAGCG TAGCTGCGAT CTGGGCCTGG GCGTTCCGTT CAATATCGCT TCCTACTCCA TTCTGACCGC TATGGTTGCC CAAGTTTGTG GTCTTGGTCT GGGTGAATTC GTTCATAACC TGGCTGATGC CCACATTTAC GTGGACCACG TTGACGCTGT GACCACCCAG ATAGCTCGTA TTCCTCACCC ATTTCCGCGC CTGCGTCTTA ATCCAGACAT TCGTAACATT GAAGACTTCA CCATCGACGA TATCGTCGTG GAAGATTACG TGTCCCACCC ACCGATTCCT ATGGCCATGT CTGCATAAAA GCTTGCGGCC GCACTCGAGC ACCACCACCA CCACCACTGA GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGAT
