SSGCID: Target Page

BaboA.01078.a

GMP reductase, putative

CENTER ID:	BaboA.01078.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.01078.a.A1.GE26567	Full length( BaboA.01078.a )		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.01078.a.A1.PW26637	Full length( BaboA.01078.a )		1	327

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_IV005560
RefSeq:	XP_001610485.1
UniProt:	A7AQU8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEIFDFDDVM LVPRMCTLET RAHADVSAKL GNRTFKIPLM ASNMPSVLDE NIVIELAQNN YFYVMHRIDV DLVEFSRKMR DLQLFVSISI GVQEQSYKVV DSLVANDLIP DYVTIDVAHG HSLAMQRMIS YIKKAFGPKT FIIAGNVATA EGVVDLENWG ADAIKVGLGP GYVCSTSIRT GFGTRNWQLA AVRECARVAK KAVIIADGGC RLSGDIVKAL YMGADWVMSG YFYAGFIKSP SETEIVDGVP MKVYYGNASA RCKKSRSRVE GVSLMVPCEE GTLLEKLKGI EEDLQSAVSF AGGRCILDVR KCEYVIVRHS RGPKESG

NT Sequence

ATGGAAATCT TCGACTTTGA CGATGTTATG CTCGTCCCAA GGATGTGTAC CTTGGAGACT AGAGCTCATG CTGACGTATC CGCTAAGCTG GGAAATCGCA CATTCAAGAT TCCACTTATG GCTTCCAATA TGCCTTCAGT TTTGGATGAG AATATTGTAA TTGAACTAGC CCAGAACAAC TACTTCTACG TTATGCATAG GATCGATGTT GACCTCGTTG AGTTTTCACG GAAAATGCGT GATCTTCAAT TGTTTGTCTC AATATCCATT GGTGTACAAG AGCAATCATA CAAGGTAGTA GACAGCCTCG TGGCAAACGA CCTCATCCCA GACTATGTCA CCATCGACGT GGCCCATGGA CATAGCCTTG CAATGCAACG TATGATTTCG TACATTAAGA AGGCATTTGG TCCAAAGACT TTTATCATCG CCGGTAACGT CGCTACTGCA GAAGGGGTGG TTGACCTAGA AAACTGGGGA GCTGATGCCA TCAAGGTTGG CCTTGGACCA GGATATGTAT GCAGCACATC TATTCGTACT GGATTCGGAA CACGTAACTG GCAATTAGCA GCCGTAAGGG AATGTGCGCG TGTGGCAAAG AAGGCTGTCA TTATTGCTGA CGGTGGATGC CGTCTCAGTG GTGATATTGT CAAAGCACTC TACATGGGAG CTGATTGGGT AATGTCAGGC TACTTCTATG CGGGATTCAT TAAGTCACCG TCGGAAACGG AAATTGTAGA TGGTGTGCCA ATGAAGGTTT ACTACGGAAA CGCCTCTGCA CGTTGTAAAA AAAGCAGGAG CCGCGTAGAA GGTGTATCGC TAATGGTTCC ATGTGAAGAG GGCACCTTAT TAGAGAAACT TAAAGGTATT GAGGAAGACT TGCAGTCAGC TGTCTCATTT GCAGGTGGAA GGTGCATCCT AGACGTACGT AAGTGCGAAT ATGTCATTGT AAGACACTCA CGTGGACCCA AGGAATCAGG A

Details for BaboA.01078.a.A1.GE26567

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	38kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Validated AA Sequence

MXHHXHHXXX TLEAQTXGPG SMEIFXFDDV MLVPRMCTLE TRAHADVSAK LGNRTFKIPL MASNMPSVLD ENIVIELAQN NYFYVMHRID VDLVEFSRKM RDLQLFVSIS IGVQEQSYKV VDSLVANDLI PDYVTIDVAH GHSLAMQRMI SYIKKAFGPK TFIIAGNVAT AEGVVDLENW GADAIKVGLG PGYVCSTSIR TGFGTRNWQL AAVRECARVA KKAVIIADGG CRLSGDIVKA LYMGADWVMS GYFYAGFIKS PSETEIVDGV PMKVYYGNAS ARCKKSRSRV EGVSLMVPCE EGTLLEKLKG IEEDLQSAVS FAGGRCILDV RKCEYVIVRH SRGPK

Validated NT Sequence

taccatggnt catcaccntc accatcntnt ggntaccctg gaagctcaga cccngggtcc tggttcgatg gaaatcttcn nntttgacga tgttatgctc gtcccaagga tgtgtacctt ggagactaga gctcatgctg acgtatccgc taagctggga aatcgcacat tcaagattcc acttatggct tccaatatgc cttcagtttt ggatgagaat attgtaattg aactagccca gaacaactac ttctacgtta tgcataggat cgatgttgac ctcgttgagt tttcacggaa aatgcgtgat cttcaattgt ttgtctcaat atccattggt gtacaagagc aatcatacaa ggtagtagac agcctcgtgg caaacgacct catcccagac tatgtcacca tcgacgtggc ccatggacat agccttgcaa tgcaacgtat gatttcgtac attaagaagg catttggtcc aaagactttt atcatcgccg gtaacgtcgc tactgcagaa ggggtggttg acctagaaaa ctggggagct gatgccatca aggttggcct tggaccagga tatgtatgca gcacatctat tcgtactgga ttcggaacac gtaactggca attagcagcc gtaagggaat gtgcgcgtgt ggcaaagaag gctgtcatta ttgctgacgg tggatgccgt ctcagtggtg atattgtcaa agcactctac atgggagctg attgggtaat gtcaggctac ttctatgcgg gattcattaa gtcaccgtcg gaaacggaaa ttgtagatgg tgtgccaatg aaggtttact acggaaacgc ctctgcacgt tgtaaaaaaa gcaggagccg cgtagaaggt gtatcgctaa tggttccatg tgaagagggc accttattag agaaacttaa aggtattgag gaagacttgc agtcagctgt ctcatttgca ggtggaaggt gcatcctaga cgtacgtaag tgcgaatatg tcattgtaag acactcacgt ggacccaagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEIFDFDDV MLVPRMCTLE TRAHADVSAK LGNRTFKIPL MASNMPSVLD ENIVIELAQN NYFYVMHRID VDLVEFSRKM RDLQLFVSIS IGVQEQSYKV VDSLVANDLI PDYVTIDVAH GHSLAMQRMI SYIKKAFGPK TFIIAGNVAT AEGVVDLENW GADAIKVGLG PGYVCSTSIR TGFGTRNWQL AAVRECARVA KKAVIIADGG CRLSGDIVKA LYMGADWVMS GYFYAGFIKS PSETEIVDGV PMKVYYGNAS ARCKKSRSRV EGVSLMVPCE EGTLLEKLKG IEEDLQSAVS FAGGRCILDV RKCEYVIVRH SRGPKESG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaatcttc gactttgacg atgttatgct cgtcccaagg atgtgtacct tggagactag agctcatgct gacgtatccg ctaagctggg aaatcgcaca ttcaagattc cacttatggc ttccaatatg ccttcagttt tggatgagaa tattgtaatt gaactagccc agaacaacta cttctacgtt atgcatagga tcgatgttga cctcgttgag ttttcacgga aaatgcgtga tcttcaattg tttgtctcaa tatccattgg tgtacaagag caatcataca aggtagtaga cagcctcgtg gcaaacgacc tcatcccaga ctatgtcacc atcgacgtgg cccatggaca tagccttgca atgcaacgta tgatttcgta cattaagaag gcatttggtc caaagacttt tatcatcgcc ggtaacgtcg ctactgcaga aggggtggtt gacctagaaa actggggagc tgatgccatc aaggttggcc ttggaccagg atatgtatgc agcacatcta ttcgtactgg attcggaaca cgtaactggc aattagcagc cgtaagggaa tgtgcgcgtg tggcaaagaa ggctgtcatt attgctgacg gtggatgccg tctcagtggt gatattgtca aagcactcta catgggagct gattgggtaa tgtcaggcta cttctatgcg ggattcatta agtcaccgtc ggaaacggaa attgtagatg gtgtgccaat gaaggtttac tacggaaacg cctctgcacg ttgtaaaaaa agcaggagcc gcgtagaagg tgtatcgcta atggttccat gtgaagaggg caccttatta gagaaactta aaggtattga ggaagacttg cagtcagctg tctcatttgc aggtggaagg tgcatcctag acgtacgtaa gtgcgaatat gtcattgtaa gacactcacg tggacccaag gaatcaggaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BaboA.01078.a.A1.PW26637

PURIFICATION DATe:	7/6/2009
CONCENTRATION:	2.02mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	27
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAATCTTC GACTTTGACG ATGTTATGCT CGTCCCAAGG ATGTGTACCT TGGAGACTAG AGCTCATGCT GACGTATCCG CTAAGCTGGG AAATCGCACA TTCAAGATTC CACTTATGGC TTCCAATATG CCTTCAGTTT TGGATGAGAA TATTGTAATT GAACTAGCCC AGAACAACTA CTTCTACGTT ATGCATAGGA TCGATGTTGA CCTCGTTGAG TTTTCACGGA AAATGCGTGA TCTTCAATTG TTTGTCTCAA TATCCATTGG TGTACAAGAG CAATCATACA AGGTAGTAGA CAGCCTCGTG GCAAACGACC TCATCCCAGA CTATGTCACC ATCGACGTGG CCCATGGACA TAGCCTTGCA ATGCAACGTA TGATTTCGTA CATTAAGAAG GCATTTGGTC CAAAGACTTT TATCATCGCC GGTAACGTCG CTACTGCAGA AGGGGTGGTT GACCTAGAAA ACTGGGGAGC TGATGCCATC AAGGTTGGCC TTGGACCAGG ATATGTATGC AGCACATCTA TTCGTACTGG ATTCGGAACA CGTAACTGGC AATTAGCAGC CGTAAGGGAA TGTGCGCGTG TGGCAAAGAA GGCTGTCATT ATTGCTGACG GTGGATGCCG TCTCAGTGGT GATATTGTCA AAGCACTCTA CATGGGAGCT GATTGGGTAA TGTCAGGCTA CTTCTATGCG GGATTCATTA AGTCACCGTC GGAAACGGAA ATTGTAGATG GTGTGCCAAT GAAGGTTTAC TACGGAAACG CCTCTGCACG TTGTAAAAAA AGCAGGAGCC GCGTAGAAGG TGTATCGCTA ATGGTTCCAT GTGAAGAGGG CACCTTATTA GAGAAACTTA AAGGTATTGA GGAAGACTTG CAGTCAGCTG TCTCATTTGC AGGTGGAAGG TGCATCCTAG ACGTACGTAA GTGCGAATAT GTCATTGTAA GACACTCACG TGGACCCAAG GAATCAGGAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A