BaboA.01035.a

succinly CoA-ligase beta subunit, putative

CENTER ID: BaboA.01035.a
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.01035.a.A1.GE26551 Full length( BaboA.01035.a ) 1 436
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.01035.a.A1.PS00329 Full length( BaboA.01035.a ) 1 436
External Resources
RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_IV010490
OrthoMCL: OG5_126862
RefSeq: XP_001610970.1
UniProt: A7AS83
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAFVGFRKA LARSIVNGKL SFKGDNASCN HARSTKRYLN VPEFGGMRIL KEHGIPTPMN RLARSPSEAE AMTHEILEST KCGEVVLKAL VLTGGRGKGK FVGTDISGVE LAKSPERAKT LAEGMIGNVL VTKQTGASGL KCKEVLVAEK LNLAKERYIS FMLDRSSCSI MAIATKHGGG NVEEVSEKDP SAVLTVKINP LKGITDDEVT KIATHLDFSK GLLEDTKSFV KNMYHAFVAK DALLLEINPL SETENSKLVA CDSKVIIDDN ADFRQKEIFE SVPPAENELE ARANEVGLNY ISLGGNIACI VNGAGLAMAT LDLLTHHGGS PSNFLDVGGN ATGHMLNQAL DIVASDAKAK ALLVNIVGGI VQCDQFALSF VEAAKNSKMQ IPVVLRLQGT NADAAKRILN EAQIPHIFCQ DFDSAAKSVV EIANAS
NT Sequence
ATGAGGGCTT TTGTAGGTTT TAGAAAAGCG CTTGCTCGTT CCATAGTCAA CGGGAAGCTT TCCTTTAAGG GTGATAACGC TTCCTGTAAT CATGCAAGAT CAACAAAACG ATACCTCAAT GTCCCAGAAT TTGGGGGTAT GAGGATTCTT AAAGAACATG GTATTCCTAC ACCCATGAAT CGTTTAGCGC GCTCCCCTAG CGAAGCAGAA GCGATGACTC ATGAAATACT TGAGAGTACA AAGTGTGGAG AAGTCGTACT TAAGGCCTTG GTGCTTACCG GAGGTAGAGG AAAGGGTAAA TTCGTAGGAA CTGATATCTC AGGAGTAGAG CTTGCTAAAT CACCCGAACG TGCAAAGACA CTCGCCGAAG GCATGATAGG CAATGTGTTG GTTACCAAGC AAACTGGAGC ATCTGGTTTG AAATGTAAAG AAGTCCTTGT AGCTGAAAAG TTGAATTTAG CTAAGGAAAG ATATATTTCA TTTATGCTTG ACAGAAGCTC ATGTTCCATT ATGGCAATAG CTACCAAACA TGGAGGTGGG AATGTAGAAG AGGTTTCCGA AAAGGATCCT TCTGCGGTGC TAACCGTGAA AATTAATCCC TTAAAAGGCA TAACTGATGA CGAAGTAACT AAAATAGCAA CCCATTTGGA TTTCTCTAAA GGTCTGTTAG AAGACACCAA ATCTTTTGTC AAGAACATGT ATCACGCATT TGTAGCTAAG GATGCTCTTC TCTTAGAAAT TAATCCTCTT TCAGAGACTG AAAATAGTAA GCTAGTGGCT TGTGATTCCA AAGTTATTAT CGACGATAAT GCTGATTTCA GGCAAAAAGA GATATTCGAA TCAGTGCCCC CTGCTGAAAA CGAATTGGAA GCGAGAGCCA ACGAAGTGGG ATTGAATTAC ATTTCTTTAG GTGGCAATAT TGCATGTATA GTTAATGGAG CTGGGTTGGC CATGGCTACA CTTGATTTGC TCACCCACCA CGGAGGTTCA CCATCTAATT TCCTAGACGT CGGAGGTAAT GCTACAGGTC ATATGCTGAA TCAGGCTCTC GATATTGTAG CTTCGGATGC AAAAGCTAAG GCTCTTCTGG TTAACATAGT AGGTGGTATC GTTCAGTGTG ACCAGTTTGC TCTGTCATTT GTAGAGGCTG CTAAGAACTC TAAAATGCAG ATCCCAGTCG TATTAAGACT CCAGGGTACG AACGCCGATG CTGCCAAAAG AATCCTCAAT GAAGCTCAAA TACCACATAT ATTCTGCCAG GATTTCGATT CCGCTGCTAA ATCAGTTGTC GAAATTGCTA ATGCATCT
Details for BaboA.01035.a.A1.GE26551
HARVESTED ON: 4/23/2009
SEQUENCED ON: 5/21/2009
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRAFVGFRK ALARSIVNGK LSFKGDNASC NHARSTKRYL NVPEFGGMRI LKEHGIPTPM NRLARSPSEA EAMTHEILES TKCGEVVLKA LVLTGGRGKG KFVGTDISGV ELAKSPERAK TLAEGMIGNV LVTKQTGASG LKCKEVLVAE KLNLAKERYI SFMLDRSSCS IMAIATKHGG GNVEEVSEKD PSAVLTVKIN PLKGITDDEV TKIATHLDFS KGLLEDTKSF VKNMYHAFVA KDALLLEINP LSETENSKLV ACDSKVIIDD NADFRQKEIF ESVPPAENEL EARANEVGLN YISLGGNIAC IVNGAGLAMA TLDLLTHHGG SPSNFLDVGG NATGHMLNQA LDIVASDAKA KALLVNIVGG IVQCDQFALS FVEAAKNSKM QIPVVLRLQG TNADAAKRIL NEAQIPHIFC QDFDSAAKSV VEIANAS
Validated NT Sequence
tttaagaagg agnnnnacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagggc ttttgtaggt tttagaaaag cgcttgctcg ttccatagtc aacgggaagc tttcctttaa gggtgataac gcttcctgta atcatgcaag atcaacaaaa cgatacctca atgtcccaga atttgggggt atgaggattc ttaaagaaca tggtattcct acacccatga atcgtttagc gcgctcccct agcgaagcag aagcgatgac tcatgaaata cttgagagta caaagtgtgg agaagtcgta cttaaggcct tggtgcttac cggaggtaga ggaaagggta aattcgtagg aactgatatc tcaggagtag agcttgctaa atcacccgaa cgtgcaaaga cactcgccga aggcatgata ggcaatgtgt tggttaccaa gcaaactgga gcatctggtt tgaaatgtaa agaagtcctt gtagctgaaa agttgaattt agctaaggaa agatatattt catttatgct tgacagaagc tcatgttcca ttatggcaat agctaccaaa catggaggtg ggaatgtaga agaggtttcc gaaaaggatc cttctgcggt gctaaccgtg aaaattaatc ccttaaaagg cataactgat gacgaagtaa ctaaaatagc aacccatttg gatttctcta aaggtctgtt agaagacacc aaatcttttg tcaagaacat gtatcacgca tttgtagcta aggatgctct tctcttagaa attaatcctc tttcagagac tgaaaatagt aagctagtgg cttgtgattc caaagttatt atcgacgata atgctgattt caggcaaaaa gagatattcg aatcagtgcc ccctgctgaa aacgaattgg aagcgagagc caacgaagtg ggattgaatt acatttcttt aggtggcaat attgcatgta tagttaatgg agctgggttg gccatggcta cacttgattt gctcacccac cacggaggtt caccatctaa tttcctagac gtcggaggta atgctacagg tcatatgctg aatcaggctc tcgatattgt agcttcggat gcaaaagcta aggctcttct ggttaacata gtaggtggta tcgttcagtg tgaccagttt gctctgtcat ttgtagaggc tgctaagaac tctaaaatgc agatcccagt cgtattaaga ctccagggta cgaacgccga tgctgccaaa agaatcctca atgaagctca aataccacat atattctgcc aggatttcga ttccgctgct aaatcagttg tcgaaattgc taatgcatct taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRAFVGFRK ALARSIVNGK LSFKGDNASC NHARSTKRYL NVPEFGGMRI LKEHGIPTPM NRLARSPSEA EAMTHEILES TKCGEVVLKA LVLTGGRGKG KFVGTDISGV ELAKSPERAK TLAEGMIGNV LVTKQTGASG LKCKEVLVAE KLNLAKERYI SFMLDRSSCS IMAIATKHGG GNVEEVSEKD PSAVLTVKIN PLKGITDDEV TKIATHLDFS KGLLEDTKSF VKNMYHAFVA KDALLLEINP LSETENSKLV ACDSKVIIDD NADFRQKEIF ESVPPAENEL EARANEVGLN YISLGGNIAC IVNGAGLAMA TLDLLTHHGG SPSNFLDVGG NATGHMLNQA LDIVASDAKA KALLVNIVGG IVQCDQFALS FVEAAKNSKM QIPVVLRLQG TNADAAKRIL NEAQIPHIFC QDFDSAAKSV VEIANAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagggctttt gtaggtttta gaaaagcgct tgctcgttcc atagtcaacg ggaagctttc ctttaagggt gataacgctt cctgtaatca tgcaagatca acaaaacgat acctcaatgt cccagaattt gggggtatga ggattcttaa agaacatggt attcctacac ccatgaatcg tttagcgcgc tcccctagcg aagcagaagc gatgactcat gaaatacttg agagtacaaa gtgtggagaa gtcgtactta aggccttggt gcttaccgga ggtagaggaa agggtaaatt cgtaggaact gatatctcag gagtagagct tgctaaatca cccgaacgtg caaagacact cgccgaaggc atgataggca atgtgttggt taccaagcaa actggagcat ctggtttgaa atgtaaagaa gtccttgtag ctgaaaagtt gaatttagct aaggaaagat atatttcatt tatgcttgac agaagctcat gttccattat ggcaatagct accaaacatg gaggtgggaa tgtagaagag gtttccgaaa aggatccttc tgcggtgcta accgtgaaaa ttaatccctt aaaaggcata actgatgacg aagtaactaa aatagcaacc catttggatt tctctaaagg tctgttagaa gacaccaaat cttttgtcaa gaacatgtat cacgcatttg tagctaagga tgctcttctc ttagaaatta atcctctttc agagactgaa aatagtaagc tagtggcttg tgattccaaa gttattatcg acgataatgc tgatttcagg caaaaagaga tattcgaatc agtgccccct gctgaaaacg aattggaagc gagagccaac gaagtgggat tgaattacat ttctttaggt ggcaatattg catgtatagt taatggagct gggttggcca tggctacact tgatttgctc acccaccacg gaggttcacc atctaatttc ctagacgtcg gaggtaatgc tacaggtcat atgctgaatc aggctctcga tattgtagct tcggatgcaa aagctaaggc tcttctggtt aacatagtag gtggtatcgt tcagtgtgac cagtttgctc tgtcatttgt agaggctgct aagaactcta aaatgcagat cccagtcgta ttaagactcc agggtacgaa cgccgatgct gccaaaagaa tcctcaatga agctcaaata ccacatatat tctgccagga tttcgattcc gctgctaaat cagttgtcga aattgctaat gcatctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BaboA.01035.a.A1.PS00329
PURIFICATION DATe: 6/24/2009
CONCENTRATION: 22.98mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRAFVGFRK ALARSIVNGK LSFKGDNASC NHARSTKRYL NVPEFGGMRI LKEHGIPTPM NRLARSPSEA EAMTHEILES TKCGEVVLKA LVLTGGRGKG KFVGTDISGV ELAKSPERAK TLAEGMIGNV LVTKQTGASG LKCKEVLVAE KLNLAKERYI SFMLDRSSCS IMAIATKHGG GNVEEVSEKD PSAVLTVKIN PLKGITDDEV TKIATHLDFS KGLLEDTKSF VKNMYHAFVA KDALLLEINP LSETENSKLV ACDSKVIIDD NADFRQKEIF ESVPPAENEL EARANEVGLN YISLGGNIAC IVNGAGLAMA TLDLLTHHGG SPSNFLDVGG NATGHMLNQA LDIVASDAKA KALLVNIVGG IVQCDQFALS FVEAAKNSKM QIPVVLRLQG TNADAAKRIL NEAQIPHIFC QDFDSAAKSV VEIANAS
Validated NT Sequence
tttaagaagg agnnnnacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagggc ttttgtaggt tttagaaaag cgcttgctcg ttccatagtc aacgggaagc tttcctttaa gggtgataac gcttcctgta atcatgcaag atcaacaaaa cgatacctca atgtcccaga atttgggggt atgaggattc ttaaagaaca tggtattcct acacccatga atcgtttagc gcgctcccct agcgaagcag aagcgatgac tcatgaaata cttgagagta caaagtgtgg agaagtcgta cttaaggcct tggtgcttac cggaggtaga ggaaagggta aattcgtagg aactgatatc tcaggagtag agcttgctaa atcacccgaa cgtgcaaaga cactcgccga aggcatgata ggcaatgtgt tggttaccaa gcaaactgga gcatctggtt tgaaatgtaa agaagtcctt gtagctgaaa agttgaattt agctaaggaa agatatattt catttatgct tgacagaagc tcatgttcca ttatggcaat agctaccaaa catggaggtg ggaatgtaga agaggtttcc gaaaaggatc cttctgcggt gctaaccgtg aaaattaatc ccttaaaagg cataactgat gacgaagtaa ctaaaatagc aacccatttg gatttctcta aaggtctgtt agaagacacc aaatcttttg tcaagaacat gtatcacgca tttgtagcta aggatgctct tctcttagaa attaatcctc tttcagagac tgaaaatagt aagctagtgg cttgtgattc caaagttatt atcgacgata atgctgattt caggcaaaaa gagatattcg aatcagtgcc ccctgctgaa aacgaattgg aagcgagagc caacgaagtg ggattgaatt acatttcttt aggtggcaat attgcatgta tagttaatgg agctgggttg gccatggcta cacttgattt gctcacccac cacggaggtt caccatctaa tttcctagac gtcggaggta atgctacagg tcatatgctg aatcaggctc tcgatattgt agcttcggat gcaaaagcta aggctcttct ggttaacata gtaggtggta tcgttcagtg tgaccagttt gctctgtcat ttgtagaggc tgctaagaac tctaaaatgc agatcccagt cgtattaaga ctccagggta cgaacgccga tgctgccaaa agaatcctca atgaagctca aataccacat atattctgcc aggatttcga ttccgctgct aaatcagttg tcgaaattgc taatgcatct taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRAFVGFRK ALARSIVNGK LSFKGDNASC NHARSTKRYL NVPEFGGMRI LKEHGIPTPM NRLARSPSEA EAMTHEILES TKCGEVVLKA LVLTGGRGKG KFVGTDISGV ELAKSPERAK TLAEGMIGNV LVTKQTGASG LKCKEVLVAE KLNLAKERYI SFMLDRSSCS IMAIATKHGG GNVEEVSEKD PSAVLTVKIN PLKGITDDEV TKIATHLDFS KGLLEDTKSF VKNMYHAFVA KDALLLEINP LSETENSKLV ACDSKVIIDD NADFRQKEIF ESVPPAENEL EARANEVGLN YISLGGNIAC IVNGAGLAMA TLDLLTHHGG SPSNFLDVGG NATGHMLNQA LDIVASDAKA KALLVNIVGG IVQCDQFALS FVEAAKNSKM QIPVVLRLQG TNADAAKRIL NEAQIPHIFC QDFDSAAKSV VEIANAS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGGGCTTTT GTAGGTTTTA GAAAAGCGCT TGCTCGTTCC ATAGTCAACG GGAAGCTTTC CTTTAAGGGT GATAACGCTT CCTGTAATCA TGCAAGATCA ACAAAACGAT ACCTCAATGT CCCAGAATTT GGGGGTATGA GGATTCTTAA AGAACATGGT ATTCCTACAC CCATGAATCG TTTAGCGCGC TCCCCTAGCG AAGCAGAAGC GATGACTCAT GAAATACTTG AGAGTACAAA GTGTGGAGAA GTCGTACTTA AGGCCTTGGT GCTTACCGGA GGTAGAGGAA AGGGTAAATT CGTAGGAACT GATATCTCAG GAGTAGAGCT TGCTAAATCA CCCGAACGTG CAAAGACACT CGCCGAAGGC ATGATAGGCA ATGTGTTGGT TACCAAGCAA ACTGGAGCAT CTGGTTTGAA ATGTAAAGAA GTCCTTGTAG CTGAAAAGTT GAATTTAGCT AAGGAAAGAT ATATTTCATT TATGCTTGAC AGAAGCTCAT GTTCCATTAT GGCAATAGCT ACCAAACATG GAGGTGGGAA TGTAGAAGAG GTTTCCGAAA AGGATCCTTC TGCGGTGCTA ACCGTGAAAA TTAATCCCTT AAAAGGCATA ACTGATGACG AAGTAACTAA AATAGCAACC CATTTGGATT TCTCTAAAGG TCTGTTAGAA GACACCAAAT CTTTTGTCAA GAACATGTAT CACGCATTTG TAGCTAAGGA TGCTCTTCTC TTAGAAATTA ATCCTCTTTC AGAGACTGAA AATAGTAAGC TAGTGGCTTG TGATTCCAAA GTTATTATCG ACGATAATGC TGATTTCAGG CAAAAAGAGA TATTCGAATC AGTGCCCCCT GCTGAAAACG AATTGGAAGC GAGAGCCAAC GAAGTGGGAT TGAATTACAT TTCTTTAGGT GGCAATATTG CATGTATAGT TAATGGAGCT GGGTTGGCCA TGGCTACACT TGATTTGCTC ACCCACCACG GAGGTTCACC ATCTAATTTC CTAGACGTCG GAGGTAATGC TACAGGTCAT ATGCTGAATC AGGCTCTCGA TATTGTAGCT TCGGATGCAA AAGCTAAGGC TCTTCTGGTT AACATAGTAG GTGGTATCGT TCAGTGTGAC CAGTTTGCTC TGTCATTTGT AGAGGCTGCT AAGAACTCTA AAATGCAGAT CCCAGTCGTA TTAAGACTCC AGGGTACGAA CGCCGATGCT GCCAAAAGAA TCCTCAATGA AGCTCAAATA CCACATATAT TCTGCCAGGA TTTCGATTCC GCTGCTAAAT CAGTTGTCGA AATTGCTAAT GCATCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA