SSGCID: Target Page

BaboA.01010.a

Cdc-related protein kinase I

CENTER ID:	BaboA.01010.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.01010.a.B1.GE36482	full length		1	505

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_II004380
RefSeq:	XP_001609961.1
UniProt:	A7ATY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASDSSEEGY LLTPEEYDVP REVKDTPIQA NSTNEDVCNV EDIPNRSNAT KEVTQADLTS SDNDEESKQR HRSSTVSPYP SNSTQEDYSR TNSDAGNQER NNRSIPDFTQ NDITAAGVVV TEMQACRNVE IYKCLNKISE GTYGSVYRAL DTETGNIVAL KHIKYHEGHW KEGFPVSYLR EISILLELRH PNVLSVKEVV TNEARDQYYV VMEYVEHELK TLLHDGKPDF TLSERKCLLY QLLRGVEYLH SHWVLHRDLK TTNILYNNKG VLKICDFGMA RKFGRPLKNY TQNVVTHWYR APELFLGQNH YDEAVDMWSV GCIFAEIICG KPMFTGSSDA DTLDKIFRCC GTPNNETWPG FSELRLVKEG KFPISKHKPN FHAFFKNNLT SNDQCYMTEA GLDLLMKMLT LNPSKRISAK EAMEHPYLTT VCLYCGFFIM MQEKPRTQAI ELMPTIPDTN AKIRVKRSPD EESNKMSRFA GRVNPVKFLA LMERNRSKKT SRQFE

NT Sequence

atggcgagtg attccagtga agaaggatat ttgttgacac cggaagaata tgatgtaccc agagaagtta aagatactcc aattcaagca aatagtacaa atgaggacgt gtgtaacgta gaagacatac ccaaccggag caatgcaact aaagaagtaa cacaggctga tcttacatca tccgacaatg atgaagaatc aaaacaacga catagaagtt caacagtgtc accctatccc tctaattcaa ctcaggagga ttattcacgc actaatagtg atgctggaaa tcaagagaga aacaatcgta gtataccgga ttttacacaa aatgacataa ctgcagctgg tgtagttgta actgaaatgc aagcttgccg taatgtagaa atttacaaat gtctaaataa aatatccgag ggaacatatg gttctgtcta tagagcccta gatacagaga ctggaaacat tgttgctttg aaacatataa aataccatga aggacactgg aaagaaggat tccctgtaag ctatttgaga gaaatatcca tattgcttga actcagacat ccaaatgtac tcagtgtaaa agaagttgtt accaatgaag ctagggatca atattatgtg gtaatggaat atgtagaaca tgaactcaaa acattgttgc atgatgggaa acccgatttc acactatcgg aacgtaagtg cttgctttat cagctgctcc gtggtgtaga atacctgcac tcccactggg ttctacaccg agatttgaaa acgacaaaca tattgtataa taataagggg gtactaaaaa tatgtgattt tggaatggcc agaaagtttg gacgacccct gaaaaattat acccaaaatg tagttacgca ttggtataga gcgcctgaac tatttcttgg ccagaatcac tacgatgaag cagtggatat gtggtcagtc ggttgtatat ttgccgaaat aatatgtgga aagccaatgt tcacgggttc atccgatgca gataccctgg ataaaatatt tagatgctgt ggaactccaa ataatgaaac gtggccagga tttagcgaat tgcgtctagt aaaagaagga aagtttccaa taagtaaaca taagcccaat ttccacgcat tcttcaagaa taatttaaca agcaatgacc aatgctacat gaccgaagca ggtctggatc tcctaatgaa aatgctaacg ttaaacccat caaaacgtat aagtgctaaa gaagcaatgg aacatcccta tcttactacg gtatgtttat actgtggttt ttttattatg atgcaggaaa aacccaggac acaggcaatc gaacttatgc ctactatacc ggatacaaat gctaaaataa gagtaaaaag atctcctgat gaagaatcaa acaagatgtc aagatttgct ggtagagtta acccagttaa gttcttagct cttatggaaa ggaatagatc aaaaaaaaca agtcggcaat ttgaataa

Details for BaboA.01010.a.B1.GE36482

HARVESTED ON:	1/9/2013
SEQUENCED ON:	1/7/2013
EXPECTED MW:	59kDa
OBSERVED MW:	59kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	65

Validated AA Sequence

Validated NT Sequence

anttttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgg cgagtgattc cagtgaagaa ggatatttgt tgacaccgga agaatatgat gtacccagag aagttaaaga tactccaatt caagcaaata gtacaaatga ggacgtgtgt aacgtagaag acatacccaa ccggagcaat gcaactaaag aagtaacaca ggctgatctt acatcatccg acaatgatga agaatcaaaa caacgacata gaagttcaac agtgtcaccc tatccctcta attcaactca ggaggattat tcacgcacta atagtgatgc tggaaatcaa gagagaaaca atcgtagtat accggatttt acacaaaatg acataactgc agctggtgta gttgtaactg aaatgcaagc ttgccgtaat gtagaaattt acaaatgtct aaataaaata tccgagggaa catatggttc tgtctataga gccctagata cagagactgg aaacattgtt gctttgaaac atataaaata ccatgaagga cactggaaag aaggattccc tgtaagctat ttgagagaaa tatccatatt gcttgaactc agacatccaa atgtactcag tgtaaaagaa gttgttacca atgaagctag ggatcaatat tatgtggtaa tggaatatgt agaacatgaa ctcaaaacat tgttgcatga tgggaaaccc gatttcacac tatcggaacg taagtgcttg ctttatcagc tgctccgtgg tgtagaatac ctgcactccc actgggttct acaccgagat ttgaaaacga caaacatatt gtataataat aagggggtac taaaaatatg tgantttgga atggccagaa agtttggacg acccctgaaa aattataccc aaaatgnagt tacgcattgg tatagagcgc ctgaactatt tcttggnnng aatcactacn atgaagcagt nnnatgnggt cagtcggtng tatatttgcc naantaatat gngnaagccn atgntnacng gttcntccga tgcnnanncc ntgnnnaaaa nanttnnntg ctgnngnncn nnannnatga ancnnttann nannnnnnna ntaaanaann naannnnnna ntannnnanc cntnnnnnnn nttnnnnnna anantnnnnn nnnnnnnann nnnnnnnncn nnnnnnnnnn nnnnnnnnan nntnnnnnnn ncnnnnnnaa

Expected Protein Sequence

MAHHHHHHMA SDSSEEGYLL TPEEYDVPRE VKDTPIQANS TNEDVCNVED IPNRSNATKE VTQADLTSSD NDEESKQRHR SSTVSPYPSN STQEDYSRTN SDAGNQERNN RSIPDFTQND ITAAGVVVTE MQACRNVEIY KCLNKISEGT YGSVYRALDT ETGNIVALKH IKYHEGHWKE GFPVSYLREI SILLELRHPN VLSVKEVVTN EARDQYYVVM EYVEHELKTL LHDGKPDFTL SERKCLLYQL LRGVEYLHSH WVLHRDLKTT NILYNNKGVL KICDFGMARK FGRPLKNYTQ NVVTHWYRAP ELFLGQNHYD EAVDMWSVGC IFAEIICGKP MFTGSSDADT LDKIFRCCGT PNNETWPGFS ELRLVKEGKF PISKHKPNFH AFFKNNLTSN DQCYMTEAGL DLLMKMLTLN PSKRISAKEA MEHPYLTTVC LYCGFFIMMQ EKPRTQAIEL MPTIPDTNAK IRVKRSPDEE SNKMSRFAGR VNPVKFLALM ERNRSKKTSR QFE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga gtgattccag tgaagaagga tatttgttga caccggaaga atatgatgta cccagagaag ttaaagatac tccaattcaa gcaaatagta caaatgagga cgtgtgtaac gtagaagaca tacccaaccg gagcaatgca actaaagaag taacacaggc tgatcttaca tcatccgaca atgatgaaga atcaaaacaa cgacatagaa gttcaacagt gtcaccctat ccctctaatt caactcagga ggattattca cgcactaata gtgatgctgg aaatcaagag agaaacaatc gtagtatacc ggattttaca caaaatgaca taactgcagc tggtgtagtt gtaactgaaa tgcaagcttg ccgtaatgta gaaatttaca aatgtctaaa taaaatatcc gagggaacat atggttctgt ctatagagcc ctagatacag agactggaaa cattgttgct ttgaaacata taaaatacca tgaaggacac tggaaagaag gattccctgt aagctatttg agagaaatat ccatattgct tgaactcaga catccaaatg tactcagtgt aaaagaagtt gttaccaatg aagctaggga tcaatattat gtggtaatgg aatatgtaga acatgaactc aaaacattgt tgcatgatgg gaaacccgat ttcacactat cggaacgtaa gtgcttgctt tatcagctgc tccgtggtgt agaatacctg cactcccact gggttctaca ccgagatttg aaaacgacaa acatattgta taataataag ggggtactaa aaatatgtga ttttggaatg gccagaaagt ttggacgacc cctgaaaaat tatacccaaa atgtagttac gcattggtat agagcgcctg aactatttct tggccagaat cactacgatg aagcagtgga tatgtggtca gtcggttgta tatttgccga aataatatgt ggaaagccaa tgttcacggg ttcatccgat gcagataccc tggataaaat atttagatgc tgtggaactc caaataatga aacgtggcca ggatttagcg aattgcgtct agtaaaagaa ggaaagtttc caataagtaa acataagccc aatttccacg cattcttcaa gaataattta acaagcaatg accaatgcta catgaccgaa gcaggtctgg atctcctaat gaaaatgcta acgttaaacc catcaaaacg tataagtgct aaagaagcaa tggaacatcc ctatcttact acggtatgtt tatactgtgg tttttttatt atgatgcagg aaaaacccag gacacaggca atcgaactta tgcctactat accggataca aatgctaaaa taagagtaaa aagatctcct gatgaagaat caaacaagat gtcaagattt gctggtagag ttaacccagt taagttctta gctcttatgg aaaggaatag atcaaaaaaa acaagtcggc aatttgaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat