BaboA.00974.a
fructose-1,6-bisphosphate aldolase
| CENTER ID: | BaboA.00974.a |
| ORGANISM: | Babesia bovis T2Bo |
| ASSOCIATED DISEASE: | Babesiosis (atypical) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaboA.00974.a.A1.GE26549 | Full length( BaboA.00974.a ) | 1 | 358 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaboA.00974.a.A1.PW26631 | Full length( BaboA.00974.a ) | 1 | 358 |
Structures
3KX6
DEPOSITED: 12/2/2009
DETERMINATION: XRay
CLONE: BaboA.00974.a.A1.GE26549
PROTEIN: BaboA.00974.a.A1.PW26631
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | PiroplasmaDB:BBOV_IV000790 |
| OrthoMCL: | OG5_127143 |
| RefSeq: | XP_001609245.1 |
| UniProt: | A7AV51 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQVKLSQERA KELAENASFI ASPGKGILAA DESTGTIQKR FDNVGVENTE KNRAEYRSIL FTTKGLGKYI SGCILFEETL FQQAPNGQNM VDLLRAEGIL PGIKVDKGLV TIPNTDEEVS TTGLDGLAER CQKYYNAGAR FAKWRAVLSI DVKKNKPSNL SILETAHTLA RYAAICQENG LVPIVEPEIL ADGDHSIEVC AEVTERVLAA VFKALNDHKV LLEGALLKPN MVTQGVDCKD KPAPQTVGFL TSRALRRTVP PALPGVMFLS GGQSESMATR HLNEINKCNK HPWSLSFSYG RALQSSVLKT WNGSMSNAAA AQDVLMKLAQ QNSEASLGSL KTDLSDDGES LFEAKYIY
NT Sequence
ATGCAGGTGA AATTGTCGCA AGAACGCGCC AAGGAACTGG CTGAAAATGC CAGCTTCATT GCTTCCCCTG GTAAAGGTAT TCTAGCTGCT GACGAGAGTA CTGGAACCAT CCAGAAACGT TTCGACAATG TGGGCGTAGA AAACACGGAA AAGAATCGTG CCGAATACCG CTCTATTCTT TTTACCACTA AAGGGTTAGG AAAGTACATT TCGGGTTGTA TTCTTTTTGA GGAAACATTG TTCCAGCAAG CCCCAAATGG ACAGAACATG GTGGATTTGT TGCGTGCTGA GGGTATATTG CCCGGTATTA AGGTTGACAA AGGTTTGGTA ACCATTCCTA ACACTGATGA AGAAGTCAGC ACCACTGGAT TGGACGGTTT GGCAGAAAGG TGCCAAAAGT ACTATAATGC CGGTGCTCGT TTCGCAAAAT GGCGTGCTGT GCTGTCAATT GATGTTAAGA AGAACAAACC ATCAAACTTG TCAATACTTG AAACTGCCCA CACACTTGCA CGTTATGCCG CCATTTGCCA GGAAAACGGT TTGGTTCCTA TTGTTGAACC CGAGATTTTG GCAGATGGAG ACCATAGCAT TGAGGTTTGT GCCGAGGTTA CCGAACGCGT TTTGGCAGCT GTGTTCAAGG CTCTTAATGA TCACAAGGTT TTGCTCGAGG GTGCTCTACT AAAGCCAAAC ATGGTTACTC AAGGTGTTGA CTGCAAGGAT AAGCCTGCTC CCCAGACAGT TGGATTTTTG ACTTCACGTG CACTTCGTCG TACCGTGCCC CCCGCTCTCC CCGGTGTTAT GTTCTTGTCT GGTGGTCAAT CTGAATCTAT GGCTACTCGC CATTTGAATG AGATCAACAA GTGCAACAAA CACCCATGGA GCCTCAGCTT CTCCTATGGC CGTGCTCTTC AGTCATCCGT ATTGAAGACT TGGAACGGTT CTATGAGCAA TGCTGCTGCT GCTCAGGATG TTCTGATGAA ATTGGCTCAG CAGAACTCCG AGGCCTCTCT TGGCAGTTTG AAGACCGATT TATCTGACGA TGGCGAATCT CTTTTTGAAG CAAAATACAT ATAC
Details for BaboA.00974.a.A1.GE26549
| HARVESTED ON: | 4/23/2009 |
| SEQUENCED ON: | 5/21/2009 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQVKLSQER AKELAENASF IASPGKGILA ADESTGTIQK RFDNVGVENT EKNRAEYRSI LFTTKGLGKY ISGCILFEET LFQQAPNGQN MVDLLRAEGI LPGIKVDKGL VTIPNTDEEV STTGLDGLAE RCQKYYNAGA RFAKWRAVLS IDVKKNKPSN LSILETAHTL ARYAAICQEN GLVPIVEPEI LADGDHSIEV CAEVTERVLA AVFKALNDHK VLLEGALLKP NMVTQGVDCK DKPAPQTVGF LTSRALRRTV PPALPGVMFL SGGQSESMAT RHLNEINKCN KHPWSLSFSY GRALQSSVLK TWNGSMSNAA AAQDVLMKLA QQNSEASLGS LKTDLSDDGE SLFEAKYIY
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcaggtg aaattgtcgc aagaacgcgc caaggaactg gctgaaaatg ccagcttcat tgcttcccct ggtaaaggta ttctagctgc tgacgagagt actggaacca tccagaaacg tttcgacaat gtgggcgtag aaaacacgga aaagaatcgt gccgaatacc gctctattct ttttaccact aaagggttag gaaagtacat ttcgggttgt attctttttg aggaaacatt gttccagcaa gccccaaatg gacagaacat ggtggatttg ttgcgtgctg agggtatatt gcccggtatt aaggttgaca aaggtttggt aaccattcct aacactgatg aagaagtcag caccactgga ttggacggtt tggcagaaag gtgccaaaag tactataatg ccggtgctcg tttcgcaaaa tggcgtgctg tgctgtcaat tgatgttaag aagaacaaac catcaaactt gtcaatactt gaaactgccc acacacttgc acgttatgcc gccatttgcc aggaaaacgg tttggttcct attgttgaac ccgagatttt ggcagatgga gaccatagca ttgaggtttg tgccgaggtt accgaacgcg ttttggcagc tgtgttcaag gctcttaatg atcacaaggt tttgctcgag ggtgctctac taaagccaaa catggttact caaggtgttg actgcaagga taagcctgct ccccagacag ttggattttt gacttcacgt gcacttcgtc gtaccgtgcc ccccgctctc cccggtgtta tgttcttgtc tggtggtcaa tctgaatcta tggctactcg ccatttgaat gagatcaaca agtgcaacaa acacccatgg agcctcagct tctcctatgg ccgtgctctt cagtcatccg tattgaagac ttggaacggt tctatgagca atgctgctgc tgctcaggat gttctgatga aattggctca gcagaactcc gaggcctctc ttggcagttt gaagaccgat ttatctgacg atggcgaatc tctttttgaa gcaaaataca tatactaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQVKLSQER AKELAENASF IASPGKGILA ADESTGTIQK RFDNVGVENT EKNRAEYRSI LFTTKGLGKY ISGCILFEET LFQQAPNGQN MVDLLRAEGI LPGIKVDKGL VTIPNTDEEV STTGLDGLAE RCQKYYNAGA RFAKWRAVLS IDVKKNKPSN LSILETAHTL ARYAAICQEN GLVPIVEPEI LADGDHSIEV CAEVTERVLA AVFKALNDHK VLLEGALLKP NMVTQGVDCK DKPAPQTVGF LTSRALRRTV PPALPGVMFL SGGQSESMAT RHLNEINKCN KHPWSLSFSY GRALQSSVLK TWNGSMSNAA AAQDVLMKLA QQNSEASLGS LKTDLSDDGE SLFEAKYIY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggtgaaa ttgtcgcaag aacgcgccaa ggaactggct gaaaatgcca gcttcattgc ttcccctggt aaaggtattc tagctgctga cgagagtact ggaaccatcc agaaacgttt cgacaatgtg ggcgtagaaa acacggaaaa gaatcgtgcc gaataccgct ctattctttt taccactaaa gggttaggaa agtacatttc gggttgtatt ctttttgagg aaacattgtt ccagcaagcc ccaaatggac agaacatggt ggatttgttg cgtgctgagg gtatattgcc cggtattaag gttgacaaag gtttggtaac cattcctaac actgatgaag aagtcagcac cactggattg gacggtttgg cagaaaggtg ccaaaagtac tataatgccg gtgctcgttt cgcaaaatgg cgtgctgtgc tgtcaattga tgttaagaag aacaaaccat caaacttgtc aatacttgaa actgcccaca cacttgcacg ttatgccgcc atttgccagg aaaacggttt ggttcctatt gttgaacccg agattttggc agatggagac catagcattg aggtttgtgc cgaggttacc gaacgcgttt tggcagctgt gttcaaggct cttaatgatc acaaggtttt gctcgagggt gctctactaa agccaaacat ggttactcaa ggtgttgact gcaaggataa gcctgctccc cagacagttg gatttttgac ttcacgtgca cttcgtcgta ccgtgccccc cgctctcccc ggtgttatgt tcttgtctgg tggtcaatct gaatctatgg ctactcgcca tttgaatgag atcaacaagt gcaacaaaca cccatggagc ctcagcttct cctatggccg tgctcttcag tcatccgtat tgaagacttg gaacggttct atgagcaatg ctgctgctgc tcaggatgtt ctgatgaaat tggctcagca gaactccgag gcctctcttg gcagtttgaa gaccgattta tctgacgatg gcgaatctct ttttgaagca aaatacatat acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BaboA.00974.a.A1.PW26631
| PURIFICATION DATe: | 6/16/2009 |
| CONCENTRATION: | 29.96mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 24 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQVKLSQER AKELAENASF IASPGKGILA ADESTGTIQK RFDNVGVENT EKNRAEYRSI LFTTKGLGKY ISGCILFEET LFQQAPNGQN MVDLLRAEGI LPGIKVDKGL VTIPNTDEEV STTGLDGLAE RCQKYYNAGA RFAKWRAVLS IDVKKNKPSN LSILETAHTL ARYAAICQEN GLVPIVEPEI LADGDHSIEV CAEVTERVLA AVFKALNDHK VLLEGALLKP NMVTQGVDCK DKPAPQTVGF LTSRALRRTV PPALPGVMFL SGGQSESMAT RHLNEINKCN KHPWSLSFSY GRALQSSVLK TWNGSMSNAA AAQDVLMKLA QQNSEASLGS LKTDLSDDGE SLFEAKYIY
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcaggtg aaattgtcgc aagaacgcgc caaggaactg gctgaaaatg ccagcttcat tgcttcccct ggtaaaggta ttctagctgc tgacgagagt actggaacca tccagaaacg tttcgacaat gtgggcgtag aaaacacgga aaagaatcgt gccgaatacc gctctattct ttttaccact aaagggttag gaaagtacat ttcgggttgt attctttttg aggaaacatt gttccagcaa gccccaaatg gacagaacat ggtggatttg ttgcgtgctg agggtatatt gcccggtatt aaggttgaca aaggtttggt aaccattcct aacactgatg aagaagtcag caccactgga ttggacggtt tggcagaaag gtgccaaaag tactataatg ccggtgctcg tttcgcaaaa tggcgtgctg tgctgtcaat tgatgttaag aagaacaaac catcaaactt gtcaatactt gaaactgccc acacacttgc acgttatgcc gccatttgcc aggaaaacgg tttggttcct attgttgaac ccgagatttt ggcagatgga gaccatagca ttgaggtttg tgccgaggtt accgaacgcg ttttggcagc tgtgttcaag gctcttaatg atcacaaggt tttgctcgag ggtgctctac taaagccaaa catggttact caaggtgttg actgcaagga taagcctgct ccccagacag ttggattttt gacttcacgt gcacttcgtc gtaccgtgcc ccccgctctc cccggtgtta tgttcttgtc tggtggtcaa tctgaatcta tggctactcg ccatttgaat gagatcaaca agtgcaacaa acacccatgg agcctcagct tctcctatgg ccgtgctctt cagtcatccg tattgaagac ttggaacggt tctatgagca atgctgctgc tgctcaggat gttctgatga aattggctca gcagaactcc gaggcctctc ttggcagttt gaagaccgat ttatctgacg atggcgaatc tctttttgaa gcaaaataca tatactaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQVKLSQER AKELAENASF IASPGKGILA ADESTGTIQK RFDNVGVENT EKNRAEYRSI LFTTKGLGKY ISGCILFEET LFQQAPNGQN MVDLLRAEGI LPGIKVDKGL VTIPNTDEEV STTGLDGLAE RCQKYYNAGA RFAKWRAVLS IDVKKNKPSN LSILETAHTL ARYAAICQEN GLVPIVEPEI LADGDHSIEV CAEVTERVLA AVFKALNDHK VLLEGALLKP NMVTQGVDCK DKPAPQTVGF LTSRALRRTV PPALPGVMFL SGGQSESMAT RHLNEINKCN KHPWSLSFSY GRALQSSVLK TWNGSMSNAA AAQDVLMKLA QQNSEASLGS LKTDLSDDGE SLFEAKYIY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGGTGAAA TTGTCGCAAG AACGCGCCAA GGAACTGGCT GAAAATGCCA GCTTCATTGC TTCCCCTGGT AAAGGTATTC TAGCTGCTGA CGAGAGTACT GGAACCATCC AGAAACGTTT CGACAATGTG GGCGTAGAAA ACACGGAAAA GAATCGTGCC GAATACCGCT CTATTCTTTT TACCACTAAA GGGTTAGGAA AGTACATTTC GGGTTGTATT CTTTTTGAGG AAACATTGTT CCAGCAAGCC CCAAATGGAC AGAACATGGT GGATTTGTTG CGTGCTGAGG GTATATTGCC CGGTATTAAG GTTGACAAAG GTTTGGTAAC CATTCCTAAC ACTGATGAAG AAGTCAGCAC CACTGGATTG GACGGTTTGG CAGAAAGGTG CCAAAAGTAC TATAATGCCG GTGCTCGTTT CGCAAAATGG CGTGCTGTGC TGTCAATTGA TGTTAAGAAG AACAAACCAT CAAACTTGTC AATACTTGAA ACTGCCCACA CACTTGCACG TTATGCCGCC ATTTGCCAGG AAAACGGTTT GGTTCCTATT GTTGAACCCG AGATTTTGGC AGATGGAGAC CATAGCATTG AGGTTTGTGC CGAGGTTACC GAACGCGTTT TGGCAGCTGT GTTCAAGGCT CTTAATGATC ACAAGGTTTT GCTCGAGGGT GCTCTACTAA AGCCAAACAT GGTTACTCAA GGTGTTGACT GCAAGGATAA GCCTGCTCCC CAGACAGTTG GATTTTTGAC TTCACGTGCA CTTCGTCGTA CCGTGCCCCC CGCTCTCCCC GGTGTTATGT TCTTGTCTGG TGGTCAATCT GAATCTATGG CTACTCGCCA TTTGAATGAG ATCAACAAGT GCAACAAACA CCCATGGAGC CTCAGCTTCT CCTATGGCCG TGCTCTTCAG TCATCCGTAT TGAAGACTTG GAACGGTTCT ATGAGCAATG CTGCTGCTGC TCAGGATGTT CTGATGAAAT TGGCTCAGCA GAACTCCGAG GCCTCTCTTG GCAGTTTGAA GACCGATTTA TCTGACGATG GCGAATCTCT TTTTGAAGCA AAATACATAT ACAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA