BaboA.00914.a

glyceraldehyde-3-phosphate dehydrogenase protein

CENTER ID: BaboA.00914.a
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.00914.a.A1.GE26547 Full length( BaboA.00914.a ) 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.00914.a.A1.PS00233 Full length( BaboA.00914.a ) 1 337
External Resources
RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_II002540
OrthoMCL: OG5_126628
RefSeq: XP_001609777.1
UniProt: A7ATE8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVVKVGINGF GRIGRLVLRA SLAYDNLEVV AINDPFMTAD YMAYLLKYDS VHGTLGETVS VTADTLKIGS RSIKLFFERE PSQIPWGQNG VDFVAECTGV FTSSEKSQQH IAGGAKLVII SAPPSDSTPI YVYGVNHTKY EKSQRIVSNA SCTTNCLAPL AKVIHEKFGI VEGLMTTVHA TTANQLTVDG ASRGGKDWRA GRCAGANIIP ASTGAAKAVG KVIPELNGKL TGMAFRVPTP DVSVVDLTCK LAKPATYDEI VSAVKAASEG ELKGILGWAD DDLVSTDFVH DKRSSIFDVK AGIALTDTFV KLVSWYDNEW GFSNRLLDLG LYICSKQ
NT Sequence
ATGGTCGTCA AGGTTGGTAT TAACGGTTTT GGCCGTATTG GTCGTTTGGT TCTCCGTGCT TCTTTGGCCT ACGACAACCT CGAGGTTGTT GCTATTAACG ACCCATTCAT GACCGCTGAT TACATGGCTT ACTTGCTGAA GTACGATTCT GTTCACGGCA CTCTTGGTGA GACCGTCTCC GTTACTGCTG ACACTCTCAA GATTGGTTCT CGTTCTATTA AATTGTTCTT CGAGCGTGAG CCTTCTCAGA TTCCATGGGG ACAGAATGGC GTAGACTTCG TTGCTGAGTG CACTGGTGTA TTCACTAGCA GTGAGAAGTC ACAACAGCAC ATTGCAGGTG GTGCCAAGTT GGTCATTATT TCTGCTCCTC CCAGCGACTC TACTCCCATT TACGTTTACG GTGTCAACCA CACTAAATAT GAGAAGTCTC AGCGCATCGT TTCGAACGCT AGTTGTACCA CAAACTGTTT GGCTCCATTG GCTAAGGTTA TTCATGAGAA GTTCGGCATT GTCGAAGGCC TTATGACCAC TGTACACGCC ACCACTGCTA ACCAGCTTAC TGTTGACGGT GCTTCCCGTG GTGGTAAGGA CTGGAGGGCT GGTCGCTGTG CCGGTGCTAA CATCATTCCT GCCTCCACCG GTGCTGCCAA GGCTGTAGGC AAGGTCATTC CCGAGTTGAA TGGTAAGCTC ACAGGTATGG CCTTCCGTGT TCCTACCCCC GATGTCAGTG TCGTTGACTT GACCTGCAAA CTCGCCAAGC CAGCTACTTA TGACGAAATT GTTAGTGCTG TTAAGGCTGC TTCTGAGGGT GAACTTAAGG GCATTCTTGG TTGGGCCGAT GATGACTTGG TTTCCACCGA TTTTGTTCAC GACAAGAGAT CTAGCATCTT TGACGTCAAG GCCGGTATTG CCCTTACCGA CACCTTCGTA AAGTTGGTTT CATGGTACGA CAATGAATGG GGATTCTCCA ACAGGCTTCT TGACTTGGGT CTCTACATCT GTTCGAAGCA G
Details for BaboA.00914.a.A1.GE26547
HARVESTED ON: 4/23/2009
SEQUENCED ON: 5/21/2009
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVVKVGING FGRIGRLVLR ASLAYDNLEV VAINDPFMTA DYMAYLLKYD SVHGTLGETV SVTADTLKIG SRSIKLFFER EPSQIPWGQN GVDFVAECTG VFTSSEKSQQ HIAGGAKLVI ISAPPSDSTP IYVYGVNHTK YEKSQRIVSN ASCTTNCLAP LAKVIHEKFG IVEGLMTTVH ATTANQLTVD GASRGGKDWR AGRCAGANII PASTGAAKAV GKVIPELNGK LTGMAFRVPT PDVSVVDLTC KLAKPATYDE IVSAVKAASE GELKGILGWA DDDLVSTDFV HDKRSSIFDV KAGIALTDTF VKLVSWYDNE WGFSNRLLDL GLYICS
Validated NT Sequence
tcgaacagat gtagagaccc aagtcaagaa gcctgttgga gaatccccat tcattgtcgt accatgaaac caactttacg aaggtgtcgg taagggcaat accggccttg acgtcaaaga tgctagatct cttgtcgtga acaaaatcgg tggaaaccaa gtcatcatcg gcccaaccaa gaatgccctt aagttcaccc tcagaagcag ccttaacagc actaacaatt tcgtcataag tagctggctt ggcgagtttg caggtcaagt caacgacact gacatcgggg gtaggaacac ggaaggccat acctgtgagc ttaccattca actcgggaat gaccttgcct acagccttgg cagcaccggt ggaggcagga atgatgttag caccggcaca gcgaccagcc ctccagtcct taccaccacg ggaagcaccg tcaacagtaa gctggttagc agtggtggcg tgtacagtgg tcataaggcc ttcgacaatg ccgaacttct catgaataac cttagccaat ggagccaaac agtttgtggt acaactagcg ttcgaaacga tgcgctgaga cttctcatat ttagtgtggt tgacaccgta aacgtaaatg ggagtagagt cgctgggagg agcagaaata atgaccaact tggcaccacc tgcaatgtgc tgttgtgact tctcactgct agtgaataca ccagtgcact cagcaacgaa gtctacgcca ttctgtcccc atggaatctg agaaggctca cgctcgaaga acaatttaat agaacgagaa ccaatcttga gagtgtcagc agtaacggag acggtctcac caagagtgcc gtgaacagaa tcgtacttca gcaagtaagc catgtaatca gcggtcatga atgggtcgtt aatagcaaca acctcgaggt tgtcgtaggc caaagaagca cggagaacca aacgaccaat acggccaaaa ccgttaatac caaccttgac gaccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtannnctcc ttcttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVKVGING FGRIGRLVLR ASLAYDNLEV VAINDPFMTA DYMAYLLKYD SVHGTLGETV SVTADTLKIG SRSIKLFFER EPSQIPWGQN GVDFVAECTG VFTSSEKSQQ HIAGGAKLVI ISAPPSDSTP IYVYGVNHTK YEKSQRIVSN ASCTTNCLAP LAKVIHEKFG IVEGLMTTVH ATTANQLTVD GASRGGKDWR AGRCAGANII PASTGAAKAV GKVIPELNGK LTGMAFRVPT PDVSVVDLTC KLAKPATYDE IVSAVKAASE GELKGILGWA DDDLVSTDFV HDKRSSIFDV KAGIALTDTF VKLVSWYDNE WGFSNRLLDL GLYICSKQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtcgtcaag gttggtatta acggttttgg ccgtattggt cgtttggttc tccgtgcttc tttggcctac gacaacctcg aggttgttgc tattaacgac ccattcatga ccgctgatta catggcttac ttgctgaagt acgattctgt tcacggcact cttggtgaga ccgtctccgt tactgctgac actctcaaga ttggttctcg ttctattaaa ttgttcttcg agcgtgagcc ttctcagatt ccatggggac agaatggcgt agacttcgtt gctgagtgca ctggtgtatt cactagcagt gagaagtcac aacagcacat tgcaggtggt gccaagttgg tcattatttc tgctcctccc agcgactcta ctcccattta cgtttacggt gtcaaccaca ctaaatatga gaagtctcag cgcatcgttt cgaacgctag ttgtaccaca aactgtttgg ctccattggc taaggttatt catgagaagt tcggcattgt cgaaggcctt atgaccactg tacacgccac cactgctaac cagcttactg ttgacggtgc ttcccgtggt ggtaaggact ggagggctgg tcgctgtgcc ggtgctaaca tcattcctgc ctccaccggt gctgccaagg ctgtaggcaa ggtcattccc gagttgaatg gtaagctcac aggtatggcc ttccgtgttc ctacccccga tgtcagtgtc gttgacttga cctgcaaact cgccaagcca gctacttatg acgaaattgt tagtgctgtt aaggctgctt ctgagggtga acttaagggc attcttggtt gggccgatga tgacttggtt tccaccgatt ttgttcacga caagagatct agcatctttg acgtcaaggc cggtattgcc cttaccgaca ccttcgtaaa gttggtttca tggtacgaca atgaatgggg attctccaac aggcttcttg acttgggtct ctacatctgt tcgaagcaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BaboA.00914.a.A1.PS00233
PURIFICATION DATe: 6/24/2009
CONCENTRATION: 26.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 21
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVVKVGING FGRIGRLVLR ASLAYDNLEV VAINDPFMTA DYMAYLLKYD SVHGTLGETV SVTADTLKIG SRSIKLFFER EPSQIPWGQN GVDFVAECTG VFTSSEKSQQ HIAGGAKLVI ISAPPSDSTP IYVYGVNHTK YEKSQRIVSN ASCTTNCLAP LAKVIHEKFG IVEGLMTTVH ATTANQLTVD GASRGGKDWR AGRCAGANII PASTGAAKAV GKVIPELNGK LTGMAFRVPT PDVSVVDLTC KLAKPATYDE IVSAVKAASE GELKGILGWA DDDLVSTDFV HDKRSSIFDV KAGIALTDTF VKLVSWYDNE WGFSNRLLDL GLYICS
Validated NT Sequence
tcgaacagat gtagagaccc aagtcaagaa gcctgttgga gaatccccat tcattgtcgt accatgaaac caactttacg aaggtgtcgg taagggcaat accggccttg acgtcaaaga tgctagatct cttgtcgtga acaaaatcgg tggaaaccaa gtcatcatcg gcccaaccaa gaatgccctt aagttcaccc tcagaagcag ccttaacagc actaacaatt tcgtcataag tagctggctt ggcgagtttg caggtcaagt caacgacact gacatcgggg gtaggaacac ggaaggccat acctgtgagc ttaccattca actcgggaat gaccttgcct acagccttgg cagcaccggt ggaggcagga atgatgttag caccggcaca gcgaccagcc ctccagtcct taccaccacg ggaagcaccg tcaacagtaa gctggttagc agtggtggcg tgtacagtgg tcataaggcc ttcgacaatg ccgaacttct catgaataac cttagccaat ggagccaaac agtttgtggt acaactagcg ttcgaaacga tgcgctgaga cttctcatat ttagtgtggt tgacaccgta aacgtaaatg ggagtagagt cgctgggagg agcagaaata atgaccaact tggcaccacc tgcaatgtgc tgttgtgact tctcactgct agtgaataca ccagtgcact cagcaacgaa gtctacgcca ttctgtcccc atggaatctg agaaggctca cgctcgaaga acaatttaat agaacgagaa ccaatcttga gagtgtcagc agtaacggag acggtctcac caagagtgcc gtgaacagaa tcgtacttca gcaagtaagc catgtaatca gcggtcatga atgggtcgtt aatagcaaca acctcgaggt tgtcgtaggc caaagaagca cggagaacca aacgaccaat acggccaaaa ccgttaatac caaccttgac gaccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtannnctcc ttcttaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVKVGING FGRIGRLVLR ASLAYDNLEV VAINDPFMTA DYMAYLLKYD SVHGTLGETV SVTADTLKIG SRSIKLFFER EPSQIPWGQN GVDFVAECTG VFTSSEKSQQ HIAGGAKLVI ISAPPSDSTP IYVYGVNHTK YEKSQRIVSN ASCTTNCLAP LAKVIHEKFG IVEGLMTTVH ATTANQLTVD GASRGGKDWR AGRCAGANII PASTGAAKAV GKVIPELNGK LTGMAFRVPT PDVSVVDLTC KLAKPATYDE IVSAVKAASE GELKGILGWA DDDLVSTDFV HDKRSSIFDV KAGIALTDTF VKLVSWYDNE WGFSNRLLDL GLYICSKQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTCGTCAAG GTTGGTATTA ACGGTTTTGG CCGTATTGGT CGTTTGGTTC TCCGTGCTTC TTTGGCCTAC GACAACCTCG AGGTTGTTGC TATTAACGAC CCATTCATGA CCGCTGATTA CATGGCTTAC TTGCTGAAGT ACGATTCTGT TCACGGCACT CTTGGTGAGA CCGTCTCCGT TACTGCTGAC ACTCTCAAGA TTGGTTCTCG TTCTATTAAA TTGTTCTTCG AGCGTGAGCC TTCTCAGATT CCATGGGGAC AGAATGGCGT AGACTTCGTT GCTGAGTGCA CTGGTGTATT CACTAGCAGT GAGAAGTCAC AACAGCACAT TGCAGGTGGT GCCAAGTTGG TCATTATTTC TGCTCCTCCC AGCGACTCTA CTCCCATTTA CGTTTACGGT GTCAACCACA CTAAATATGA GAAGTCTCAG CGCATCGTTT CGAACGCTAG TTGTACCACA AACTGTTTGG CTCCATTGGC TAAGGTTATT CATGAGAAGT TCGGCATTGT CGAAGGCCTT ATGACCACTG TACACGCCAC CACTGCTAAC CAGCTTACTG TTGACGGTGC TTCCCGTGGT GGTAAGGACT GGAGGGCTGG TCGCTGTGCC GGTGCTAACA TCATTCCTGC CTCCACCGGT GCTGCCAAGG CTGTAGGCAA GGTCATTCCC GAGTTGAATG GTAAGCTCAC AGGTATGGCC TTCCGTGTTC CTACCCCCGA TGTCAGTGTC GTTGACTTGA CCTGCAAACT CGCCAAGCCA GCTACTTATG ACGAAATTGT TAGTGCTGTT AAGGCTGCTT CTGAGGGTGA ACTTAAGGGC ATTCTTGGTT GGGCCGATGA TGACTTGGTT TCCACCGATT TTGTTCACGA CAAGAGATCT AGCATCTTTG ACGTCAAGGC CGGTATTGCC CTTACCGACA CCTTCGTAAA GTTGGTTTCA TGGTACGACA ATGAATGGGG ATTCTCCAAC AGGCTTCTTG ACTTGGGTCT CTACATCTGT TCGAAGCAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A