BaboA.00748.a

casein kinase II, alpha chain (CK II)

CENTER ID:	BaboA.00748.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.00748.a.A1.GE26545	Full length( BaboA.00748.a )		1	423

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_III007790
OrthoMCL:	OG5_126901
RefSeq:	XP_001611907.1
UniProt:	A7AP55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLTGGVWKL YLASNLLFAA FHSGVSLKPV SANEVGTAQS GAPRGQQRKP LAFRGSIRIF VNPEGYIEES ENDEPSPTNM DVESEKRATS RGTYPNTNLP IILPKLYADV NTKRGPDYWD YENITLMWNV PNNYEIVRKL GRGKYSEVFE GTNLLTHERI VVKILKPLKK KKIKREIKIL QNLSGGPNII KLLDIVKDPQ SRTPSLIFEY VNNTDFKILY PTFTIADIKY YMYQLLKAVD YAHSQGIMHR DVKPHNVMID HEKRELKLID WGLAEFYHPE QEYNVRVASR YYKGPELLVD MKYYDYSLDV WSIGCMLAGM VFKKEPFFYG HDNYDQLVKI AKVLGTEDLR AYFKKYGLKV APVYEKILGN YPRKPWSSFI EDENKHLCVP EVIDLIDKML VYDHTMRITP KDAMDHPFFK DVA

NT Sequence

ATGAAACTCA CCGGTGGAGT ATGGAAGCTA TATTTGGCTT CAAATTTGTT GTTTGCGGCA TTCCATAGCG GTGTGTCACT AAAACCAGTG AGCGCGAACG AGGTTGGCAC TGCCCAATCA GGAGCGCCCC GTGGACAACA AAGGAAGCCT CTAGCCTTTC GGGGATCTAT CCGAATTTTT GTAAATCCGG AGGGTTATAT AGAAGAGAGT GAGAACGACG AACCAAGTCC AACTAATATG GATGTCGAGA GTGAGAAACG CGCGACATCA CGCGGAACAT ATCCAAACAC AAATTTGCCC ATCATCCTTC CTAAGCTATA TGCAGATGTT AACACTAAAC GTGGTCCTGA TTATTGGGAC TATGAAAACA TTACACTCAT GTGGAATGTC CCTAACAACT ATGAGATCGT GAGGAAGCTT GGACGCGGCA AGTACAGCGA GGTTTTTGAA GGTACAAATC TCCTTACACA CGAACGTATA GTTGTCAAGA TTCTTAAGCC ATTGAAAAAG AAAAAAATCA AGAGGGAAAT CAAGATTCTA CAAAACCTCA GCGGGGGGCC AAACATCATT AAGCTATTAG ACATCGTCAA GGACCCTCAG AGCAGAACTC CATCGCTTAT ATTTGAATAC GTCAACAACA CGGATTTCAA AATCTTGTAT CCAACATTCA CGATCGCAGA TATCAAGTAC TACATGTACC AGCTGCTCAA GGCCGTGGAC TATGCTCACA GCCAAGGTAT CATGCACAGA GATGTGAAGC CGCATAACGT CATGATTGAC CATGAAAAGA GGGAATTGAA ACTAATTGAC TGGGGTTTGG CCGAGTTCTA CCACCCTGAA CAGGAATACA ACGTAAGAGT TGCAAGTAGA TATTACAAGG GACCTGAGCT ACTAGTAGAC ATGAAGTACT ACGACTATAG CTTGGATGTA TGGAGTATTG GATGTATGTT GGCAGGAATG GTATTCAAAA AGGAACCGTT CTTTTATGGA CACGACAATT ACGACCAGCT TGTTAAGATT GCAAAGGTTC TAGGAACCGA GGACCTACGT GCCTACTTCA AGAAGTACGG ACTTAAGGTG GCTCCAGTAT ACGAAAAAAT ACTGGGCAAT TATCCGCGCA AGCCGTGGTC TAGTTTCATC GAGGATGAAA ATAAACACCT TTGTGTGCCA GAAGTCATCG ATCTTATAGA TAAGATGCTG GTGTACGACC ACACCATGAG AATCACACCA AAGGATGCAA TGGACCACCC TTTCTTCAAA GATGTGGCC

Details for BaboA.00748.a.A1.GE26545

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	49

Validated AA Sequence

MYQLLKAVDY AHSQGIMHRD VKPHNVMIDH EKRELKLIDW GLAEFYHPEQ EYNVRVASRY YKGPELLVDM KYYDYSLDVW SXGCMLAGMV FKKEPFFYGH DNYDQLVKIA KVLGTEDLRA YFKKYGLKVA PVYEKILGNY PRKPWSSFIE DENKHLCVPE VIDLIDKMLV YDHTMRITPK DAMDHPFFKD VA

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKLTGGVWK LYLASNLLFA AFHSGVSLKP VSANEVGTAQ SGAPRGQQRK PLAFRGSIRI FVNPEGYIEE SENDEPSPTN MDVESEKRAT SRGTYPNTNL PIILPKLYAD VNTKRGPDYW DYENITLMWN VPNNYEIVRK LGRGKYSEVF EGTNLLTHER IVVKILKPLK KKKIKREIKI LQNLSGGPNI IKLLDIVKDP QSRTPSLIFE YVNNTDFKIL YPTFTIADIK YYMYQLLKAV DYAHSQGIMH RDVKPHNVMI DHEKRELKLI DWGLAEFYHP EQEYNVRVAS RYYKGPELLV DMKYYDYSLD VWSIGCMLAG MVFKKEPFFY GHDNYDQLVK IAKVLGTEDL RAYFKKYGLK VAPVYEKILG NYPRKPWSSF IEDENKHLCV PEVIDLIDKM LVYDHTMRIT PKDAMDHPFF KDVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaactcacc ggtggagtat ggaagctata tttggcttca aatttgttgt ttgcggcatt ccatagcggt gtgtcactaa aaccagtgag cgcgaacgag gttggcactg cccaatcagg agcgccccgt ggacaacaaa ggaagcctct agcctttcgg ggatctatcc gaatttttgt aaatccggag ggttatatag aagagagtga gaacgacgaa ccaagtccaa ctaatatgga tgtcgagagt gagaaacgcg cgacatcacg cggaacatat ccaaacacaa atttgcccat catccttcct aagctatatg cagatgttaa cactaaacgt ggtcctgatt attgggacta tgaaaacatt acactcatgt ggaatgtccc taacaactat gagatcgtga ggaagcttgg acgcggcaag tacagcgagg tttttgaagg tacaaatctc cttacacacg aacgtatagt tgtcaagatt cttaagccat tgaaaaagaa aaaaatcaag agggaaatca agattctaca aaacctcagc ggggggccaa acatcattaa gctattagac atcgtcaagg accctcagag cagaactcca tcgcttatat ttgaatacgt caacaacacg gatttcaaaa tcttgtatcc aacattcacg atcgcagata tcaagtacta catgtaccag ctgctcaagg ccgtggacta tgctcacagc caaggtatca tgcacagaga tgtgaagccg cataacgtca tgattgacca tgaaaagagg gaattgaaac taattgactg gggtttggcc gagttctacc accctgaaca ggaatacaac gtaagagttg caagtagata ttacaaggga cctgagctac tagtagacat gaagtactac gactatagct tggatgtatg gagtattgga tgtatgttgg caggaatggt attcaaaaag gaaccgttct tttatggaca cgacaattac gaccagcttg ttaagattgc aaaggttcta ggaaccgagg acctacgtgc ctacttcaag aagtacggac ttaaggtggc tccagtatac gaaaaaatac tgggcaatta tccgcgcaag ccgtggtcta gtttcatcga ggatgaaaat aaacaccttt gtgtgccaga agtcatcgat cttatagata agatgctggt gtacgaccac accatgagaa tcacaccaaa ggatgcaatg gaccaccctt tcttcaaaga tgtggccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa