BaboA.00728.a

actin

CENTER ID: BaboA.00728.a
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.00728.a.B1.GE26929 Full length( BaboA.00728.a ) 1 376
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_I000300
OrthoMCL: OG5_126595
RefSeq: XP_001608692.1
UniProt: A7AX51
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDEEATALV IDNGSGNVKA GVAGDDAPRC VFPSIVGRPK NPALMVGMDE KDTYVGDEAQ AKRGILTLKY PIEHGIVTNW EDMEKIWHHT FYNELRIAPE EHPVLLTEAP MNPKANREKM TTIMFETHNV PAMYVAIQAV LSLYSSGRTT GIVLDSGDGV THTVPIYEGY ALPHAIMRID LAGRDLTEFM QKILAERGFT FTTTAEKEIV RDIKEKLCYI ALDFEEEMNS SASSSEIEKS YELPDGNIIT VGNERFRCPE VLFQPSFIGQ ESAGIHTTTF NSIARCDVDI RKDLYANVVL SGGTTMYEGI GQRMTKELNA LVPSTMKIKV VAPPERKYSV WIGGSILSSL STFQQMWITK EEFDESGPNI VHRKCF
NT Sequence
ATGTCTGACG AAGAGGCTAC TGCGTTGGTC ATTGACAATG GATCCGGTAA TGTCAAGGCT GGTGTCGCTG GTGATGACGC TCCCCGTTGT GTGTTCCCCA GCATTGTAGG TCGTCCAAAG AACCCTGCCC TTATGGTTGG CATGGATGAA AAGGACACCT ACGTTGGAGA TGAAGCCCAG GCTAAGCGTG GTATCTTGAC CCTTAAGTAC CCGATTGAGC ACGGTATCGT CACCAACTGG GAAGACATGG AAAAGATATG GCATCACACC TTCTACAATG AACTCCGTAT TGCTCCTGAG GAACACCCCG TGCTCCTTAC TGAGGCTCCC ATGAACCCTA AGGCCAACCG TGAGAAGATG ACCACCATTA TGTTCGAGAC CCACAACGTC CCAGCTATGT ACGTCGCTAT CCAGGCTGTG CTTTCCCTGT ACTCATCTGG TCGTACCACT GGTATTGTGC TCGACTCTGG TGATGGTGTT ACTCACACCG TGCCCATCTA TGAGGGTTAT GCCCTTCCTC ACGCCATTAT GCGTATTGAC CTTGCTGGTC GTGACTTAAC TGAGTTCATG CAAAAAATAT TGGCCGAACG TGGCTTTACA TTTACTACTA CTGCCGAAAA GGAAATTGTG CGTGACATTA AGGAAAAGCT TTGTTACATT GCACTTGACT TCGAAGAGGA AATGAACTCT TCTGCATCAT CTAGTGAAAT CGAGAAATCT TACGAATTGC CTGATGGAAA CATCATTACC GTTGGAAACG AGCGTTTCAG GTGCCCTGAG GTGCTCTTCC AGCCTAGCTT CATCGGTCAG GAATCTGCCG GTATCCACAC CACCACCTTC AACTCCATTG CTCGTTGTGA TGTCGACATT CGCAAGGACC TTTACGCAAA CGTCGTTCTT TCTGGAGGTA CAACTATGTA CGAGGGTATA GGACAGCGTA TGACGAAAGA ACTCAACGCA CTTGTGCCCA GCACCATGAA GATCAAGGTC GTTGCACCAC CCGAGCGCAA ATACTCTGTA TGGATTGGTG GTTCTATTCT TTCTTCACTT TCTACCTTCC AGCAAATGTG GATCACCAAG GAAGAGTTCG ATGAGTCTGG ACCCAACATC GTCCACAGGA AGTGCTTC
Details for BaboA.00728.a.B1.GE26929
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/9/2009
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 83
Validated AA Sequence
MVGMDEKDTY VGDEAQAKRG ILTLKYPIEH GIVTNWEDME KIWHHTFYNE LRIAPEEHPX LLTXAPXXPK AXRXXMTTIX FXTHXXPXMX XAXXAVXXLY SXXRXXXXXX XXXXGVXXXV XIYEXXXXPX XIXXIDLAXX DLTEFMQKIL XERGFTFTTT AEKEIVRDIK EKLCYIALDF EEEMNSSASS SEIEKSYELP DGNIITVGNE RFRXPEVLFQ PSFIGQESAG IHTTTFNSIA RCDVDIRKDL YANVVLSGGT TMYEGIGQRM TKELNALVPS TMKIKVVAPP ERKYSVWIGG SILSSLSTFQ QMWITKEEFD ES
Validated NT Sequence
tccagactca tcgaactctt ccttggtgat ccacatttgc tggaaggtag aaagtgaaga aagaatagaa ccaccaatcc atacagagta tttgcgctcg ggtggtgcaa cgaccttgat cttcatggtg ctgggcacaa gtgcgttgag ttctttcgtc atacgctgtc ctataccctc gtacatagtt gtacctccag aaagaacgac gtttgcgtaa aggtccttgc gaatgtcgac atcacaacga gcaatggagt tgaaggtggt ggtgtggata ccggcagatt cctgaccgat gaagctaggc tggaagagca cctcaggnca cctgaaacgc tcgtttccaa cggtaatgat gtttccatca ggcaattcgt aagatttctc gatttcacta gatgatgcag aagagttcat ttcctcttcg aagtcaagtg caatgtaaca aagcttttcc ttaatgtcac gcacaatttc cttttcggca gtagtagtaa atgtaaagcc acgttcgncc aatatttttt gcatgaactc agttaagtcn ngancagcaa ggtcaatacn nntaatgncg ngaggnannn cntanncctc atagatgnnn acgnngngag naacaccnnn nnnagnnnng annncaanan nnnngnnncg nncannngag tacaggnaaa ncacagcctn nntngcgang nacatagntg ggangtngtg ggtctngaac nnaatggtgg tcatnttcnc acgnntggcc ttagggtnna tgggagcntc agtaaggagc nnggggtgtt cctcaggagc aatncggagt tcattgtaga aggtgtgatg ccatatcttt tccatgtctt cccagttngt nacgataccg tgctcaatcg ggtacttaag ggtcaagata ccacgcttag cctgggcttc atctccaacg taggtgtcct tttcatccat gccaaccata agggcagggt tctttggacg acctacaatg ctggggaaca cacaacgggg agcgtcatca ccagcgacac cagccttgac attaccggat ccattgtcaa tgaccaacgc agtagcctct tnnt
Expected Protein Sequence
MAHHHHHHMS DEEATALVID NGSGNVKAGV AGDDAPRCVF PSIVGRPKNP ALMVGMDEKD TYVGDEAQAK RGILTLKYPI EHGIVTNWED MEKIWHHTFY NELRIAPEEH PVLLTEAPMN PKANREKMTT IMFETHNVPA MYVAIQAVLS LYSSGRTTGI VLDSGDGVTH TVPIYEGYAL PHAIMRIDLA GRDLTEFMQK ILAERGFTFT TTAEKEIVRD IKEKLCYIAL DFEEEMNSSA SSSEIEKSYE LPDGNIITVG NERFRCPEVL FQPSFIGQES AGIHTTTFNS IARCDVDIRK DLYANVVLSG GTTMYEGIGQ RMTKELNALV PSTMKIKVVA PPERKYSVWI GGSILSSLST FQQMWITKEE FDESGPNIVH RKCF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg acgaagaggc tactgcgttg gtcattgaca atggatccgg taatgtcaag gctggtgtcg ctggtgatga cgctccccgt tgtgtgttcc ccagcattgt aggtcgtcca aagaaccctg cccttatggt tggcatggat gaaaaggaca cctacgttgg agatgaagcc caggctaagc gtggtatctt gacccttaag tacccgattg agcacggtat cgtcaccaac tgggaagaca tggaaaagat atggcatcac accttctaca atgaactccg tattgctcct gaggaacacc ccgtgctcct tactgaggct cccatgaacc ctaaggccaa ccgtgagaag atgaccacca ttatgttcga gacccacaac gtcccagcta tgtacgtcgc tatccaggct gtgctttccc tgtactcatc tggtcgtacc actggtattg tgctcgactc tggtgatggt gttactcaca ccgtgcccat ctatgagggt tatgcccttc ctcacgccat tatgcgtatt gaccttgctg gtcgtgactt aactgagttc atgcaaaaaa tattggccga acgtggcttt acatttacta ctactgccga aaaggaaatt gtgcgtgaca ttaaggaaaa gctttgttac attgcacttg acttcgaaga ggaaatgaac tcttctgcat catctagtga aatcgagaaa tcttacgaat tgcctgatgg aaacatcatt accgttggaa acgagcgttt caggtgccct gaggtgctct tccagcctag cttcatcggt caggaatctg ccggtatcca caccaccacc ttcaactcca ttgctcgttg tgatgtcgac attcgcaagg acctttacgc aaacgtcgtt ctttctggag gtacaactat gtacgagggt ataggacagc gtatgacgaa agaactcaac gcacttgtgc ccagcaccat gaagatcaag gtcgttgcac cacccgagcg caaatactct gtatggattg gtggttctat tctttcttca ctttctacct tccagcaaat gtggatcacc aaggaagagt tcgatgagtc tggacccaac atcgtccaca ggaagtgctt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat