BaboA.00641.a

casein kinase I, putative

CENTER ID:	BaboA.00641.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.00641.a.A1.GE26541	Full length( BaboA.00641.a )		1	336

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_III002900
OrthoMCL:	OG5_126780
RefSeq:	XP_001611422.1
UniProt:	A7AMS0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MISRSLEIRV GGKYRLGRKI GSGSFGDIFM GTQIQTGEDV AIKLESHRSR HPQLLYESKL YRLLAGGVGI PTIHWYGIEG EYNILIMDLL GPSLEDLFTI CNRKLSLKSV LMLADQMLNR IEYCHSKNFI HRDIKPDNFL IGRGKKISIV YIIDFGLAKK YRDPKTGQHI CYREGKNLTG TARYASINTH LGIEQSRRDD LEALGYVLLY FMRGSLPWQG LKANSKKDKY DKISEKKIAI PVDLLCKQLP FEFVTFINYA RSLRFEDRPD YPYLRRILKD LFFRQGYQYD FIFDWTFLHT AQLMHTMVNE DIERFERMNR ERDAEEPATR PALINN

NT Sequence

ATGATCTCGA GATCGTTGGA GATTCGCGTT GGTGGGAAGT ATCGTCTCGG ACGTAAGATT GGTAGCGGCT CATTTGGCGA TATCTTTATG GGCACGCAGA TTCAAACAGG CGAGGACGTT GCCATCAAAC TTGAATCACA CCGCAGCAGG CACCCTCAGC TTCTATATGA ATCAAAGCTA TATCGTCTTC TAGCCGGTGG AGTGGGTATA CCCACCATCC ACTGGTATGG CATCGAAGGT GAGTACAACA TCCTCATTAT GGATTTGCTC GGTCCCTCCC TGGAGGACCT CTTCACTATT TGTAATCGCA AGCTTAGTTT AAAGAGTGTA CTGATGCTTG CCGACCAGAT GCTGAACCGT ATAGAATATT GCCACTCCAA GAACTTTATT CATCGTGATA TTAAACCCGA TAACTTTTTG ATCGGTCGCG GTAAGAAAAT ATCGATAGTT TACATAATCG ACTTTGGATT GGCCAAGAAA TATCGTGACC CAAAGACTGG TCAGCACATT TGTTACCGTG AGGGCAAGAA TCTTACAGGT ACAGCTCGTT ACGCCAGCAT CAACACTCAT TTGGGTATTG AGCAAAGCCG CCGTGATGAT TTGGAGGCCT TGGGTTACGT TCTTCTGTAT TTCATGCGTG GATCACTTCC ATGGCAGGGC TTGAAGGCTA ACAGCAAGAA GGATAAGTAC GACAAGATCA GCGAGAAAAA GATCGCGATA CCGGTTGATT TATTATGTAA GCAGTTGCCC TTTGAGTTCG TCACGTTTAT TAACTATGCG CGCTCACTGC GCTTCGAGGA TCGTCCTGAT TACCCGTACC TACGCCGGAT ACTGAAGGAC CTGTTTTTCA GGCAAGGCTA TCAATACGAC TTCATTTTTG ACTGGACATT CTTACACACT GCCCAGTTGA TGCATACGAT GGTAAACGAG GACATTGAAC GGTTTGAGCG CATGAATCGG GAGCGTGATG CTGAGGAGCC TGCCACCCGC CCTGCTTTGA TTAATAAT

Details for BaboA.00641.a.A1.GE26541

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMISRSLEIR VGGKYRLGRK IGSSSFGDIF MGTQIQTGED VAIKLESHRS RHPQLLYESK LYRLLAGGVG IPTIHWYGIE GEYNILIMDL LGPSLEDLFT ICNRKLSLKS VLMLADQMLN RIEYCHSKNF IHRDIKPDNF LIGRGKKISI VYIIDFGLAK KYRDPKTGQH ICYREGKNLT GTARYASINT HLGIEQSRRD DLEALGYVLL YFMRGSLPWQ GLKANSKKDK YDKISEKKIA IPVDLLCKQL PFEFVTFINY ARSLRFEDRP DYPYLRRILK DLFFRQGYQY DFIFDWTFLH TAQLMHTMVN EDIERFERMN RERDAEEPAT RPALI

Validated NT Sequence

taatcaaagc agggcgggtg gcaggctcct cagcatcacg ctcccgattc atgcgctcaa accgttcaat gtcctcgttt accatcgtat gcatcaactg ggcagtgtgt aagaatgtcc agtcaaaaat gaagtcgtat tgatagcctt gcctgaaaaa caggtccttc agtatccggc gtaggtacgg gtaatcagga cgatcctcga agcgcagtga gcgcgcatag ttaataaacg tgacgaactc aaagggcaac tgcttacata ataaatcaac cggtatcgcg atctttttct cgctgatctt gtcgtactta tccttcttgc tgttagcctt caagccctgc catggaagtg atccacgcat gaaatacaga agaacgtaac ccaaggcctc caaatcatca cggcggcttt gctcaatacc caaatgagtg ttgatgctgg cgtaacgagc tgtacctgta agattcttgc cctcacggta acaaatgtgc tgaccagtct ttgggtcacg atatttcttg gccaatccaa agtcgattat gtaaactatc gatattttct taccgcgacc gatcaaaaag ttatcgggtt taatatcacg atgaataaag ttcttggagt ggcaatattc tatacggttc agcatctggt cggcaagcat cagtacactc tttaaactaa gcttgcgatt acaaatagtg aagaggtcct ccagggaggg accgagcaaa tccataatga ggatgttgta ctcaccttcg atgccatacc agtggatggt gggtataccc actccaccgg ctagaagacg atatagcttt gattcatata gaagctgagg gtgcctgctg cggtgtgatt caagtttgat ggcaacgtcc tcgcctgttt gaatctgcgt gcccataaag atatcgccaa atgagctgct accaatctta cgtccgagac gatacttccc accaacgcga atctccaacg atctcgagat catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMISRSLEIR VGGKYRLGRK IGSGSFGDIF MGTQIQTGED VAIKLESHRS RHPQLLYESK LYRLLAGGVG IPTIHWYGIE GEYNILIMDL LGPSLEDLFT ICNRKLSLKS VLMLADQMLN RIEYCHSKNF IHRDIKPDNF LIGRGKKISI VYIIDFGLAK KYRDPKTGQH ICYREGKNLT GTARYASINT HLGIEQSRRD DLEALGYVLL YFMRGSLPWQ GLKANSKKDK YDKISEKKIA IPVDLLCKQL PFEFVTFINY ARSLRFEDRP DYPYLRRILK DLFFRQGYQY DFIFDWTFLH TAQLMHTMVN EDIERFERMN RERDAEEPAT RPALINN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatctcgaga tcgttggaga ttcgcgttgg tgggaagtat cgtctcggac gtaagattgg tagcggctca tttggcgata tctttatggg cacgcagatt caaacaggcg aggacgttgc catcaaactt gaatcacacc gcagcaggca ccctcagctt ctatatgaat caaagctata tcgtcttcta gccggtggag tgggtatacc caccatccac tggtatggca tcgaaggtga gtacaacatc ctcattatgg atttgctcgg tccctccctg gaggacctct tcactatttg taatcgcaag cttagtttaa agagtgtact gatgcttgcc gaccagatgc tgaaccgtat agaatattgc cactccaaga actttattca tcgtgatatt aaacccgata actttttgat cggtcgcggt aagaaaatat cgatagttta cataatcgac tttggattgg ccaagaaata tcgtgaccca aagactggtc agcacatttg ttaccgtgag ggcaagaatc ttacaggtac agctcgttac gccagcatca acactcattt gggtattgag caaagccgcc gtgatgattt ggaggccttg ggttacgttc ttctgtattt catgcgtgga tcacttccat ggcagggctt gaaggctaac agcaagaagg ataagtacga caagatcagc gagaaaaaga tcgcgatacc ggttgattta ttatgtaagc agttgccctt tgagttcgtc acgtttatta actatgcgcg ctcactgcgc ttcgaggatc gtcctgatta cccgtaccta cgccggatac tgaaggacct gtttttcagg caaggctatc aatacgactt catttttgac tggacattct tacacactgc ccagttgatg catacgatgg taaacgagga cattgaacgg tttgagcgca tgaatcggga gcgtgatgct gaggagcctg ccacccgccc tgctttgatt aataataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa