BaboA.00612.a

lysine-tRNA ligase, putative

CENTER ID: BaboA.00612.a
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.00612.a.A1.GE34081 full length 1 522
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_II001160
RefSeq: XP_001609643.1
UniProt: A7AT14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSRMIKPIY PQTRPMLLYE STNDYNVGTL ADAFTSLAND AVKEQMSIRL AKLKHLESEL GSSGYPEIDS KETESMTTVE QINKNHRTLN NDEENEGVNY CIYGRIKAIR FGGLFVVIED KQGDELQVLF RRKMTIHIGD KPFNAENIDQ LIDIGDVIQV GGHVKKTGTG ELTLIPHHMS ILAKCLLPMP DGFHGLKDVA TRHKLRHLDI MVNEDTRGIL LQRSHIIWEL RKLLHSKGFV EVETPILQHI YSGANAQPFK TRSEALDENL YLRIAPEFFL KRLIVGGLGD KIFEIGKCFR NEGTSARHSP EFTMIEIYQQ MANAGDMINL LEEIIETLAK KIGVEFPKFT RKTMVGLIEE HIGINVDLCS SEQLLSILRE RGLDTNTLES NSWGSLVATL FKCAVEDNIT EPTHVTELPS DISPLAASEG SKGKVFESYL QGLEIAHGCT EECNPVQLLH KLDMCGLQGL GHTRDTELLN AVAHGMPPTA GIGIGIDRLV MALSKTTSIK NIQTFPLLKS EA
NT Sequence
atgtctagta ggatgataaa gcccatatac ccacaaacta ggcctatgct gttgtatgaa agcactaatg actacaatgt tggaactctg gcagatgcat ttacaagtct agctaacgac gccgtcaagg agcaaatgtc aattcgcctg gcaaaattga aacatctgga atctgaactt ggatcttctg gttatccaga aatagacagc aaagaaacag aatccatgac cactgtggaa caaataaata aaaaccatag aacgctaaat aatgatgaag aaaatgaagg tgttaactat tgcatttacg gaaggattaa agctatacgc ttcggcggtt tgtttgtggt aattgaagat aaacagggtg atgaactaca ggttctattc cggaggaaga tgactataca cattggagat aaacccttta atgcagaaaa tatagaccaa cttattgata tcggtgatgt aatccaagta ggggggcatg tgaagaaaac gggtactggg gagttaacct tgatacctca ccatatgtca atcctagcaa aatgtctgct gcctatgcct gatggattcc acggtctcaa agacgtcgcc actagacaca agttgcgtca tttagatatt atggtaaatg aagatactcg gggaatatta ttgcaacgtt cacatatcat ctgggaatta cgcaaactgt tacatagtaa ggggtttgtt gaggttgaga ccccaatcct gcagcatata tacagcggtg cgaatgcaca gccgtttaaa acacgctcgg aggcattgga tgaaaacctc tatctacgaa ttgcaccaga atttttcctc aaacgtctta tcgtgggcgg actgggtgac aagatatttg aaatcggtaa atgcttccgt aatgagggaa ccagcgctag gcactctcca gaatttacaa tgatagaaat ataccagcaa atggccaacg cgggagatat gataaacctt ctagaagaaa taatagaaac ccttgctaag aaaataggag tggaattccc taaattcact aggaaaacaa tggttggtct catagaagaa catataggca taaatgtcga cttgtgcagt agtgagcagc tattgtcaat attgagagaa cgtggcctag acactaacac tttagaaagt aatagctggg gaagcttagt tgcaaccctc ttcaagtgcg ccgtagaaga taatatcacg gagcctacgc atgtcactga actgcctagt gatatatcac ccttggcagc aagcgaaggt tcaaagggca aagtgttcga atcatatcta caaggtttgg aaatagccca tgggtgtacg gaagagtgta accccgtcca attgttgcat aaactagata tgtgtggcct acaggggttg ggacatacac gagataccga gttactaaac gctgttgctc atggtatgcc gccaacagct ggaattggaa taggcataga ccgattggtt atggcactgt ccaagaccac cagcattaag aatatacaaa cattcccact tttgaaaagc gaagcatga
Details for BaboA.00612.a.A1.GE34081
HARVESTED ON: 6/4/2012
SEQUENCED ON: 6/5/2012
EXPECTED MW: 60kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 95
Validated AA Sequence
MSSRMIKPIY PQTRPMLLYE STNDYNVGTL ADAFTSLAND AVKEQMSIRL AKLKHLESEL GSSGYPEIDS KETESMTTVE QINKNHRTLN NDEENEGVNY CIYGRIKAIR FGGLFVVIED KQGDELQVLF RRKMTIHIGD KPFNAENIDQ LIDIGDVIQV GGHVKKTGTG ELTLIPHHMS ILAKCLLPMP DGFHGLKDVA XRHKLRHLDI MVNEDTRGIL LQRSHIIWEL RKLLHSKGFV EVETPILQHI YSGANAQPFK TRSEAXDENL YLRIAPEFFL KRLIVGGLGD XIFEIGKCFX NEGTSARHSP EFTMIEIYQQ MANAGDMINL LEEIIETLAK KIGVEFXKFT RKTMVGLIEE HIGINVDXCS SEQXLSILRE RGLDTNTLES NSWGSLVATL FKCAVEDNIT EPTHVTELPS DISPLAASEG SKGKVFESYL QGLEIAHGCT EECNPVQLLH KLDMCGLQGL GHTRDTELLN AVAHGMPPTA GIGIGIDRLV MALSKTTSIK NIQTFPLLKS
Validated NT Sequence
cgcttttcaa aagtgggaat gtttgtatat tcttaatgct ggtggtcttg gacagtgcca taaccaatcg gtctatgcct attccaattc cagctgttgg cggcatacca tgagcaacag cgtttagtaa ctcggtatct cgtgtatgtc ccaacccctg taggccacac atatctagtt tatgcaacaa ttggacgggg ttacactctt ccgtacaccc atgggctatt tccaaacctt gtagatatga ttcgaacact ttgccctttg aaccttcgct tgctgccaag ggtgatatat cactaggcag ttcagtgaca tgcgtaggct ccgtgatatt atcttctacg gcgcacttga agagggttgc aactaagctt ccccagctat tactttctaa agtgttagtg tctaggccac gttctctcaa tattgacaat anctgctcac tactgcacan gtcgacattt atgcctatat gttcttctat gagaccaacc attgttttcc tagtgaattt anggaattcc actcctattt tcttagcaag ggtttctatt atttcttcta gaaggtttat catatctccc gcgttggcca tttgctggta tatttctatc attgtaaatt ctggagagtg cctagcgctg gttccctcat tacngaagca tttaccgatt tcaaatatct ngtcacccag tccgcccacg ataagacgtt tgaggaaaaa ttctggtgca attcgtagat agaggttttc atccnatgcc tccgagcgtg ttttaaacgg ctgtgcattc gcaccgctgt atatatgctg caggattggg gtctcaacct caacaaaccc cttactatgt aacagtttgc gtaattccca gatgatatgt gaacgttgca ataatattcc ccgagtatct tcatttacca taatatctaa atgacgcaac ttgtgtctag nggcgacgtc tttgagaccg tggaatccat caggcatagg cagcagacat tttgctagga ttgacatatg gtgaggtatc aaggttaact ccccagtacc cgttttcttc acatgccccc ctacttggat tacatcaccg atatcaataa gttggtctat attttctgca ttaaagggtt tatctccaat gtgtatagtc atcttcctcc ggaatagaac ctgtagttca tcaccctgtt tatcttcaat taccacaaac aaaccgccga agcgtatagc tttaatcctt ccgtaaatgc aatagttaac accttcattt tcttcatcat tatttagcgt tctatggttt ttatttattt gttccacagt ggtcatggat tctgtttctt tgctgtctat ttctggataa ccagaagatc caagttcaga ttccagatgt ttcaattttg ccaggcgaat tgacatttgc tccttgacgg cgtcgttagc tagacttgta aatgcatctg ccagagttcc aacattgtag tcattagtgc tttcatacaa cagcataggc ctagtttgtg ggtatatggg ctttatcatc ctactagaca tcgaaccagg accctgggtc tgagcttcca gggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSRMIKPI YPQTRPMLLY ESTNDYNVGT LADAFTSLAN DAVKEQMSIR LAKLKHLESE LGSSGYPEID SKETESMTTV EQINKNHRTL NNDEENEGVN YCIYGRIKAI RFGGLFVVIE DKQGDELQVL FRRKMTIHIG DKPFNAENID QLIDIGDVIQ VGGHVKKTGT GELTLIPHHM SILAKCLLPM PDGFHGLKDV ATRHKLRHLD IMVNEDTRGI LLQRSHIIWE LRKLLHSKGF VEVETPILQH IYSGANAQPF KTRSEALDEN LYLRIAPEFF LKRLIVGGLG DKIFEIGKCF RNEGTSARHS PEFTMIEIYQ QMANAGDMIN LLEEIIETLA KKIGVEFPKF TRKTMVGLIE EHIGINVDLC SSEQLLSILR ERGLDTNTLE SNSWGSLVAT LFKCAVEDNI TEPTHVTELP SDISPLAASE GSKGKVFESY LQGLEIAHGC TEECNPVQLL HKLDMCGLQG LGHTRDTELL NAVAHGMPPT AGIGIGIDRL VMALSKTTSI KNIQTFPLLK SEA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctagtagg atgataaagc ccatataccc acaaactagg cctatgctgt tgtatgaaag cactaatgac tacaatgttg gaactctggc agatgcattt acaagtctag ctaacgacgc cgtcaaggag caaatgtcaa ttcgcctggc aaaattgaaa catctggaat ctgaacttgg atcttctggt tatccagaaa tagacagcaa agaaacagaa tccatgacca ctgtggaaca aataaataaa aaccatagaa cgctaaataa tgatgaagaa aatgaaggtg ttaactattg catttacgga aggattaaag ctatacgctt cggcggtttg tttgtggtaa ttgaagataa acagggtgat gaactacagg ttctattccg gaggaagatg actatacaca ttggagataa accctttaat gcagaaaata tagaccaact tattgatatc ggtgatgtaa tccaagtagg ggggcatgtg aagaaaacgg gtactgggga gttaaccttg atacctcacc atatgtcaat cctagcaaaa tgtctgctgc ctatgcctga tggattccac ggtctcaaag acgtcgccac tagacacaag ttgcgtcatt tagatattat ggtaaatgaa gatactcggg gaatattatt gcaacgttca catatcatct gggaattacg caaactgtta catagtaagg ggtttgttga ggttgagacc ccaatcctgc agcatatata cagcggtgcg aatgcacagc cgtttaaaac acgctcggag gcattggatg aaaacctcta tctacgaatt gcaccagaat ttttcctcaa acgtcttatc gtgggcggac tgggtgacaa gatatttgaa atcggtaaat gcttccgtaa tgagggaacc agcgctaggc actctccaga atttacaatg atagaaatat accagcaaat ggccaacgcg ggagatatga taaaccttct agaagaaata atagaaaccc ttgctaagaa aataggagtg gaattcccta aattcactag gaaaacaatg gttggtctca tagaagaaca tataggcata aatgtcgact tgtgcagtag tgagcagcta ttgtcaatat tgagagaacg tggcctagac actaacactt tagaaagtaa tagctgggga agcttagttg caaccctctt caagtgcgcc gtagaagata atatcacgga gcctacgcat gtcactgaac tgcctagtga tatatcaccc ttggcagcaa gcgaaggttc aaagggcaaa gtgttcgaat catatctaca aggtttggaa atagcccatg ggtgtacgga agagtgtaac cccgtccaat tgttgcataa actagatatg tgtggcctac aggggttggg acatacacga gataccgagt tactaaacgc tgttgctcat ggtatgccgc caacagctgg aattggaata ggcatagacc gattggttat ggcactgtcc aagaccacca gcattaagaa tatacaaaca ttcccacttt tgaaaagcga agcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa