SSGCID: Target Page

BaboA.00436.a

cAMP-dependent protein kinase

CENTER ID:	BaboA.00436.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.00436.a.B1.GE26926	Full length( BaboA.00436.a )		1	416

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_I004190
RefSeq:	XP_001609068.1
UniProt:	A7AWS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGVTYSITNG IDTGMGAYEN KNHVNKETSY SVVQSTRRLP QPQTAPRISR FMARCNNIAK HMICTNDSMA SDVRYKAWTE SDTISDGTME IIPSLPEKLD ISNFDIGPIV GTGSYATVCI ATLNTGPKSQ RATFALKILH KTKVIAERQV MHLRNERHIL STVRHPFIVN YVKSFQDDIN VYILLEYVSG GELFSYIYKY GALGHTVARF YTAQAILAID FLHFKGIVYR DLKPENMLLD QDGHIRLVDF GFAKEVKHKT YTVCGTHDYL APEIFLRCGH GIEADWWSLG VLVYEILTGV PPFHASDPSA TYDLALANHI RFPLSVRVEV KDFIRRLLVV NPRKRLGGSN SSELYSHRFF AGMDWMKLLS KQLKPPIVPR VSGLSDTTNY MQYPDDWRAH DARITRSQQD EYFSDF

NT Sequence

ATGGGCGTCA CGTATTCCAT TACCAATGGC ATCGATACAG GCATGGGCGC TTATGAAAAC AAAAATCATG TAAACAAGGA GACATCTTAC AGCGTTGTCC AATCAACAAG AAGGTTACCT CAACCACAGA CTGCTCCGAG GATTTCGAGG TTTATGGCTC GGTGCAACAA TATCGCAAAG CACATGATTT GTACAAACGA TTCTATGGCC TCAGATGTAC GTTATAAGGC ATGGACAGAA TCAGATACAA TAAGCGACGG TACTATGGAA ATAATACCAT CGCTACCTGA GAAACTGGAC ATTTCAAATT TTGATATAGG ACCAATAGTA GGAACTGGGT CATATGCTAC AGTGTGCATT GCGACTCTCA ATACTGGTCC GAAGTCTCAG CGTGCAACCT TCGCACTAAA GATTTTGCAT AAAACAAAAG TCATCGCTGA ACGACAGGTA ATGCATCTAC GTAATGAGAG ACACATTTTG TCAACCGTCA GACATCCTTT CATAGTAAAT TATGTGAAGA GTTTTCAAGA CGATATAAAT GTCTATATAT TGTTAGAATA TGTTTCTGGA GGAGAATTAT TCAGTTACAT ATATAAATAT GGAGCCCTAG GGCACACCGT TGCTCGATTC TATACTGCAC AAGCGATATT AGCTATTGAT TTTTTACACT TCAAGGGTAT AGTGTATCGT GATTTAAAGC CTGAAAATAT GCTACTTGAT CAGGACGGCC ATATACGTCT AGTTGACTTT GGATTTGCCA AGGAGGTAAA GCACAAAACA TACACGGTAT GTGGTACTCA TGACTATCTT GCTCCTGAAA TATTCCTACG TTGTGGACAC GGTATAGAAG CAGATTGGTG GTCACTAGGT GTATTAGTAT ACGAAATTTT GACGGGTGTT CCTCCATTTC ATGCTTCCGA CCCTAGTGCA ACTTATGACT TAGCGTTGGC GAACCACATT CGGTTTCCGC TGTCTGTGAG GGTTGAGGTC AAGGACTTCA TACGTCGTTT GCTAGTGGTG AACCCGCGAA AGCGTCTTGG TGGTAGTAAC AGCTCCGAGC TGTACTCCCA CAGGTTCTTC GCGGGTATGG ATTGGATGAA ATTGCTCAGT AAGCAGTTGA AGCCTCCTAT AGTACCACGT GTATCTGGTT TATCAGACAC TACTAACTAT ATGCAGTATC CAGATGACTG GCGTGCGCAT GATGCGCGTA TAACGAGGTC ACAACAAGAT GAATACTTTA GCGACTTT

Details for BaboA.00436.a.B1.GE26926

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/9/2009
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	75

Validated AA Sequence

MAHHHHHHMG VTYSITNGID TGMGAYENKN HVNKETSYSV VQSTRRLPQP QTAPRISRFM ARCNNIAKHM ICTNDSMASD VRYKAWTESD TISDGTMEII PSLPEKLDIS NFDIGPIVGT GSYATVCIAT LNTGPKSQRA TFALKILHKT KVIAERQVMH LRNERHILST VRHPFIVNYV KSFQDDINVY ILLEYVSGGE LFSYIYKYGA LGHTVARFYT AQAILAIDFL HFKGIVYRDL KPENMLLDQD GHIRLVDFGF AKEVKHKTYT VCGTHDYLAP EIFLRCGHXX EAXWWSLGXX VYXIXTGXPX FHAXDPXATX D

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg tcacgtattc cattaccaat ggcatcgata caggcatggg cgcttatgaa aacaaaaatc atgtaaacaa ggagacatct tacagcgttg tccaatcaac aagaaggtta cctcaaccac agactgctcc gaggatttcg aggtttatgg ctcggtgcaa caatatcgca aagcacatga tttgtacaaa cgattctatg gcctcagatg tacgttataa ggcatggaca gaatcagata caataagcga cggtactatg gaaataatac catcgctacc tgagaaactg gacatttcaa attttgatat aggaccaata gtaggaactg ggtcatatgc tacagtgtgc attgcgactc tcaatactgg tccgaagtct cagcgtgcaa ccttcgcact aaagattttg cataaaacaa aagtcatcgc tgaacgacag gtaatgcatc tacgtaatga gagacacatt ttgtcaaccg tcagacatcc tttcatagta aattatgtga agagttttca agacgatata aatgtctata tattgttaga atatgtttct ggaggagaat tattcagtta catatataaa tatggagccc tagggcacac cgttgctcga ttctatactg cacaagcgat attagctatt gattttttac acttcaaggg tatagtgtat cgtgatttaa agcctgaaaa tatgctactt gatcaggacg gccatatacg tctagttgac tttggatttg ccaaggaggt aaagcacaaa acatacacgg tatgtggtac tcatgactat cttgctcctg aaatattcct acgttgtgga cacggtatag aagcagattg gtggtcacta ggtgtattag tatacgaaat tttgacgggt gttcctccat ttcatgcttc cgaccctagt gcaacttatg acttagcgtt ggcgaaccac attcggtttc cgctgtctgt gagggttgag gtcaaggact tcatacgtcg tttgctagtg gtgaacccgc gaaagcgtct tggtggtagt aacagctccg agctgtactc ccacaggttc ttcgcgggta tggattggat gaaattgctc agtaagcagt tgaagcctcc tatagtacca cgtgtatctg gtttatcaga cactactaac tatatgcagt atccagatga ctggcgtgcg catgatgcgc gtataacgag gtcacaacaa gatgaatact ttagcgactt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat