BaboA.00409.b

proteasome subunit alpha type 4, subunit

CENTER ID: BaboA.00409.b
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.00409.b.A1.GE34089 full length 1 257
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.00409.b.A1.PS01559 full length 1 257

External Resources

RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_III009190
RefSeq: XP_001612044.1
UniProt: Q2PKF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRRYDTKTT TFSQEGRLYQ VEYALEAINN ANLTVGLLCD LGVVLAADKP ISTPLLDPGK INEKLYKLDS HMFCAVAGLT ADATVLINTC KLYAQRHRYA YGEPQDVEQH VVQICNLKQS YTQFGGLRPF GVSFLFAGWD SHLGFQLYHT DPSGNYSGWK ATAIGHNSQP AQSMLRQEWK EGMNLDEALY LAVKVLTKSM DSTMPKADKI EVGMLWRGPN GDGEPTVEMV SESRVAEILS KLTDEAAAKA VESGHSI
NT Sequence
atgtcgcgtc gttacgatac taagactact accttttctc aggagggtcg tttgtatcag gtggagtacg cattagaggc cattaacaat gcgaatttga ctgttggttt gttgtgtgac ctcggtgtag tattggctgc tgataaacca atcagtactc cactactaga ccctggtaag ataaatgaga agttgtacaa gttggattct cacatgtttt gtgctgttgc tggtttgact gctgatgcca ctgtattgat aaacacttgc aagctgtatg ctcagaggca caggtacgct tacggcgagc ctcaggatgt tgagcagcat gttgtacaga tatgtaattt gaagcagagt tacactcaat ttggtggttt acgtccattt ggcgttagct ttttatttgc tggttgggac agtcatttag gttttcagct ttatcacact gatccctctg gtaactattc tggttggaag gctactgcca taggtcacaa cagtcagcca gctcaatcca tgttacgtca ggagtggaag gaaggtatga accttgatga ggccttatat ttggccgtta aggtgcttac taaatctatg gactctacta tgccaaaagc tgacaagatt gaggttggta tgttatggcg cggtcctaat ggcgacggcg agcctacagt tgagatggtt tcggagtctc gcgttgctga gatactatct aagttgactg atgaagcagc tgccaaggct gttgagtcag gccactcgat atga
Details for BaboA.00409.b.A1.GE34089
HARVESTED ON: 6/4/2012
SEQUENCED ON: 6/5/2012
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SISRRYDTKT TTFSQEGRLY QVEYALEAIN NANLTVGLLC DLGVVLAADK PISTPLLDPG KINEKLYKLD SHMFCAVAGL TADATVLINT CKLYAQRHRY AYGEPQDVEQ HVVQICNLKQ SYTQFGGLRP FGVSFLFAGW DSHLGFQLYH TDPSGNYSGW KATAIGHNSQ PAQSMLRQEW KEGMNLDEAL YLAVKVLTKS MDSTMPKADK IEVGMLWRGP NGDGEPTVEM VSESRVAEIL SKLTDEAAGK AVESGHSI
Validated NT Sequence
ttattatatc gagtggcctg actcaacagc cttgccagct gcttcatcag tcaacttaga tagtatctca gcaacgcgag actccgaaac catctcaact gtaggctcgc cgtcgccatt aggaccgcgc cataacatac caacctcaat cttgtcagct tttggcatag tagagtccat agatttagta agcaccttaa cggccaaata taaggcctca tcaaggttca taccttcctt ccactcctga cgtaacatgg attgagctgg ctgactgttg tgacctatgg cagtagcctt ccaaccagaa tagttaccag agggatcagt gtgataaagc tgaaaaccta aatgactgtc ccaaccagca aataaaaagc taacgccaaa tggacgtaaa ccaccaaatt gagtgtaact ctgcttcaaa ttacatatct gtacaacatg ctgctcaaca tcctgaggct cgccgtaagc gtacctgtgc ctctgagcat acagcttgca agtgtttatc aatacagtgg catcagcagt caaaccagca acagcacaaa acatgtgaga atccaacttg tacaacttct catttatctt accagggtct agtagtggag tactgattgg tttatcagca gccaatacta caccgaggtc acacaacaaa ccaacagtca aattcgcatt gttaatggcc tctaatgcgt actccacctg atacaaacga ccctcctgag aaaaggtagt agtcttagta tcgtaacgac gcgatatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRRYDTKT TTFSQEGRLY QVEYALEAIN NANLTVGLLC DLGVVLAADK PISTPLLDPG KINEKLYKLD SHMFCAVAGL TADATVLINT CKLYAQRHRY AYGEPQDVEQ HVVQICNLKQ SYTQFGGLRP FGVSFLFAGW DSHLGFQLYH TDPSGNYSGW KATAIGHNSQ PAQSMLRQEW KEGMNLDEAL YLAVKVLTKS MDSTMPKADK IEVGMLWRGP NGDGEPTVEM VSESRVAEIL SKLTDEAAAK AVESGHSI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcgtcgt tacgatacta agactactac cttttctcag gagggtcgtt tgtatcaggt ggagtacgca ttagaggcca ttaacaatgc gaatttgact gttggtttgt tgtgtgacct cggtgtagta ttggctgctg ataaaccaat cagtactcca ctactagacc ctggtaagat aaatgagaag ttgtacaagt tggattctca catgttttgt gctgttgctg gtttgactgc tgatgccact gtattgataa acacttgcaa gctgtatgct cagaggcaca ggtacgctta cggcgagcct caggatgttg agcagcatgt tgtacagata tgtaatttga agcagagtta cactcaattt ggtggtttac gtccatttgg cgttagcttt ttatttgctg gttgggacag tcatttaggt tttcagcttt atcacactga tccctctggt aactattctg gttggaaggc tactgccata ggtcacaaca gtcagccagc tcaatccatg ttacgtcagg agtggaagga aggtatgaac cttgatgagg ccttatattt ggccgttaag gtgcttacta aatctatgga ctctactatg ccaaaagctg acaagattga ggttggtatg ttatggcgcg gtcctaatgg cgacggcgag cctacagttg agatggtttc ggagtctcgc gttgctgaga tactatctaa gttgactgat gaagcagctg ccaaggctgt tgagtcaggc cactcgatat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BaboA.00409.b.A1.PS01559
PURIFICATION DATe: 7/12/2012
CONCENTRATION: 17.69mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SISRRYDTKT TTFSQEGRLY QVEYALEAIN NANLTVGLLC DLGVVLAADK PISTPLLDPG KINEKLYKLD SHMFCAVAGL TADATVLINT CKLYAQRHRY AYGEPQDVEQ HVVQICNLKQ SYTQFGGLRP FGVSFLFAGW DSHLGFQLYH TDPSGNYSGW KATAIGHNSQ PAQSMLRQEW KEGMNLDEAL YLAVKVLTKS MDSTMPKADK IEVGMLWRGP NGDGEPTVEM VSESRVAEIL SKLTDEAAGK AVESGHSI
Validated NT Sequence
ttattatatc gagtggcctg actcaacagc cttgccagct gcttcatcag tcaacttaga tagtatctca gcaacgcgag actccgaaac catctcaact gtaggctcgc cgtcgccatt aggaccgcgc cataacatac caacctcaat cttgtcagct tttggcatag tagagtccat agatttagta agcaccttaa cggccaaata taaggcctca tcaaggttca taccttcctt ccactcctga cgtaacatgg attgagctgg ctgactgttg tgacctatgg cagtagcctt ccaaccagaa tagttaccag agggatcagt gtgataaagc tgaaaaccta aatgactgtc ccaaccagca aataaaaagc taacgccaaa tggacgtaaa ccaccaaatt gagtgtaact ctgcttcaaa ttacatatct gtacaacatg ctgctcaaca tcctgaggct cgccgtaagc gtacctgtgc ctctgagcat acagcttgca agtgtttatc aatacagtgg catcagcagt caaaccagca acagcacaaa acatgtgaga atccaacttg tacaacttct catttatctt accagggtct agtagtggag tactgattgg tttatcagca gccaatacta caccgaggtc acacaacaaa ccaacagtca aattcgcatt gttaatggcc tctaatgcgt actccacctg atacaaacga ccctcctgag aaaaggtagt agtcttagta tcgtaacgac gcgatatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRRYDTKT TTFSQEGRLY QVEYALEAIN NANLTVGLLC DLGVVLAADK PISTPLLDPG KINEKLYKLD SHMFCAVAGL TADATVLINT CKLYAQRHRY AYGEPQDVEQ HVVQICNLKQ SYTQFGGLRP FGVSFLFAGW DSHLGFQLYH TDPSGNYSGW KATAIGHNSQ PAQSMLRQEW KEGMNLDEAL YLAVKVLTKS MDSTMPKADK IEVGMLWRGP NGDGEPTVEM VSESRVAEIL SKLTDEAAAK AVESGHSI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGCGTCGT TACGATACTA AGACTACTAC CTTTTCTCAG GAGGGTCGTT TGTATCAGGT GGAGTACGCA TTAGAGGCCA TTAACAATGC GAATTTGACT GTTGGTTTGT TGTGTGACCT CGGTGTAGTA TTGGCTGCTG ATAAACCAAT CAGTACTCCA CTACTAGACC CTGGTAAGAT AAATGAGAAG TTGTACAAGT TGGATTCTCA CATGTTTTGT GCTGTTGCTG GTTTGACTGC TGATGCCACT GTATTGATAA ACACTTGCAA GCTGTATGCT CAGAGGCACA GGTACGCTTA CGGCGAGCCT CAGGATGTTG AGCAGCATGT TGTACAGATA TGTAATTTGA AGCAGAGTTA CACTCAATTT GGTGGTTTAC GTCCATTTGG CGTTAGCTTT TTATTTGCTG GTTGGGACAG TCATTTAGGT TTTCAGCTTT ATCACACTGA TCCCTCTGGT AACTATTCTG GTTGGAAGGC TACTGCCATA GGTCACAACA GTCAGCCAGC TCAATCCATG TTACGTCAGG AGTGGAAGGA AGGTATGAAC CTTGATGAGG CCTTATATTT GGCCGTTAAG GTGCTTACTA AATCTATGGA CTCTACTATG CCAAAAGCTG ACAAGATTGA GGTTGGTATG TTATGGCGCG GTCCTAATGG CGACGGCGAG CCTACAGTTG AGATGGTTTC GGAGTCTCGC GTTGCTGAGA TACTATCTAA GTTGACTGAT GAAGCAGCTG CCAAGGCTGT TGAGTCAGGC CACTCGATAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA