BaboA.00409.a

proteasome component PRE3 precursor, putative

CENTER ID: BaboA.00409.a
ORGANISM: Babesia bovis T2Bo
ASSOCIATED DISEASE: Babesiosis (atypical)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.00409.a.A1.GE34090 full length 1 266
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaboA.00409.a.A1.PS01590 full length 1 266
BaboA.00409.a.A1.PS01591 full length 1 266

External Resources

RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BBOV_II005970
RefSeq: XP_001610115.1
UniProt: A7AUD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMVDCSYYDG ISEAGIAQEC VSGSTAADDR AHDISMGTTI IAMIYRDGIL LAADSRTSSG PIVVNRVARK ITRILPNVFM LRSGSAADSQ MLSVIVRYHA QALCLQLRKS GRASGDDAIG SDDAMQIDSE MPTESEYSYR FDESMVYPPL KALATATHKI VHEYREQLYC GIILGGYDMQ HGPEIYNVTL GGTLLKVPHF VASGSGSGYI TAFLHDNYEP DMSRDQCLSL LKRAIKYAID IDNSSGGLMR AVSVSRTGVS EFCFTF
NT Sequence
atgatggtag attgtagtta ttatgatggc atttcggaag ccggaatcgc acaggagtgt gtgtctggct ccaccgcggc tgacgatcgt gctcatgaca taagcatggg tacgactatt atcgcgatga tttatcgtga tggtatactt ttggctgcgg atagtcgtac atcatctggt cctattgttg taaatcgtgt ggctcgcaag attactcgca ttcttccaaa tgtttttatg ctacgttccg gttcagctgc cgattcgcag atgctatctg ttatagtacg ttatcatgca caggctttat gtctacaatt gcgcaagagc ggacgtgcgt ctggtgatga tgctattggt agtgacgatg cgatgcagat tgacagcgaa atgcctactg agagtgaata tagttacaga ttcgacgaga gtatggttta ccctcctctt aaagcactgg ctactgcgac acacaagatt gtacatgagt accgtgagca gctgtactgt ggtataatat tgggaggtta tgatatgcaa cacggtcccg agatatacaa tgtgacattg ggtggcaccc tgttgaaagt accacacttt gtggctagtg gatcgggttc aggatacatt acagcatttt tacacgacaa ctacgagcct gacatgtctc gcgatcagtg tttgtcactg ctaaagaggg ctatcaagta cgcgattgac atcgataata gttcaggcgg tctaatgcgt gcggtatctg tttctcgtac cggcgtgtct gagttttgct tcactttcta g
Details for BaboA.00409.a.A1.GE34090
HARVESTED ON: 6/4/2012
SEQUENCED ON: 6/5/2012
EXPECTED MW: 31kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMVDCSYYD GISEAGIAQE CVSGSTAADD RAHDISMGTT IIAMIYRDGI LLAADSRTSS GPIVVNRVAR KITRILPNVF MLRSGSAADS QMLSVIVRYH AQALCLQLRK SGRASGDDAI GSDDAMQIDS EMPTESEYSY RFDESMVYPP LKALATATHK IVHEYREQLY CGIILGGYDM QHGPEIYNVT LGGTLLKVPH FVASGSGSGY ITAFLHDNYE PDMSRDQCLS LLKRAIKYAI DIDNSSGGLM RAVSVSRTGV SEFCFTF
Validated NT Sequence
ttattagaaa gtgaagcaaa actcagacac gccggtacga gaaacagata ccgcacgcat tagaccgcct gaactattat cgatgtcaat cgcgtacttg atagccctct ttagcagtga caaacactga tcgcgagaca tgtcaggctc gtagttgtcg tgtaaaaatg ctgtaatgta tcctgaaccc gatccactag ccacaaagtg tggtactttc aacagggtgc cacccaatgt cacattgtat atctcgggac cgtgttgcat atcataacct cccaatatta taccacagta cagctgctca cggtactcat gtacaatctt gtgtgtcgca gtagccagtg ctttaagagg agggtaaacc atactctcgt cgaatctgta actatattca ctctcagtag gcatttcgct gtcaatctgc atcgcatcgt cactaccaat agcatcatca ccagacgcac gtccgctctt gcgcaattgt agacataaag cctgtgcatg ataacgtact ataacagata gcatctgcga atcggcagct gaaccggaac gtagcataaa aacatttgga agaatgcgag taatcttgcg agccacacga tttacaacaa taggaccaga tgatgtacga ctatccgcag ccaaaagtat accatcacga taaatcatcg cgataatagt cgtacccatg cttatgtcat gagcacgatc gtcagccgcg gtggagccag acacacactc ctgtgcgatt ccggcttccg aaatgccatc ataataacta caatctacca tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMVDCSYYD GISEAGIAQE CVSGSTAADD RAHDISMGTT IIAMIYRDGI LLAADSRTSS GPIVVNRVAR KITRILPNVF MLRSGSAADS QMLSVIVRYH AQALCLQLRK SGRASGDDAI GSDDAMQIDS EMPTESEYSY RFDESMVYPP LKALATATHK IVHEYREQLY CGIILGGYDM QHGPEIYNVT LGGTLLKVPH FVASGSGSGY ITAFLHDNYE PDMSRDQCLS LLKRAIKYAI DIDNSSGGLM RAVSVSRTGV SEFCFTF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatggtagat tgtagttatt atgatggcat ttcggaagcc ggaatcgcac aggagtgtgt gtctggctcc accgcggctg acgatcgtgc tcatgacata agcatgggta cgactattat cgcgatgatt tatcgtgatg gtatactttt ggctgcggat agtcgtacat catctggtcc tattgttgta aatcgtgtgg ctcgcaagat tactcgcatt cttccaaatg tttttatgct acgttccggt tcagctgccg attcgcagat gctatctgtt atagtacgtt atcatgcaca ggctttatgt ctacaattgc gcaagagcgg acgtgcgtct ggtgatgatg ctattggtag tgacgatgcg atgcagattg acagcgaaat gcctactgag agtgaatata gttacagatt cgacgagagt atggtttacc ctcctcttaa agcactggct actgcgacac acaagattgt acatgagtac cgtgagcagc tgtactgtgg tataatattg ggaggttatg atatgcaaca cggtcccgag atatacaatg tgacattggg tggcaccctg ttgaaagtac cacactttgt ggctagtgga tcgggttcag gatacattac agcattttta cacgacaact acgagcctga catgtctcgc gatcagtgtt tgtcactgct aaagagggct atcaagtacg cgattgacat cgataatagt tcaggcggtc taatgcgtgc ggtatctgtt tctcgtaccg gcgtgtctga gttttgcttc actttctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BaboA.00409.a.A1.PS01590
PURIFICATION DATe: 8/7/2012
CONCENTRATION: 43.89mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMVDCSYYD GISEAGIAQE CVSGSTAADD RAHDISMGTT IIAMIYRDGI LLAADSRTSS GPIVVNRVAR KITRILPNVF MLRSGSAADS QMLSVIVRYH AQALCLQLRK SGRASGDDAI GSDDAMQIDS EMPTESEYSY RFDESMVYPP LKALATATHK IVHEYREQLY CGIILGGYDM QHGPEIYNVT LGGTLLKVPH FVASGSGSGY ITAFLHDNYE PDMSRDQCLS LLKRAIKYAI DIDNSSGGLM RAVSVSRTGV SEFCFTF
Validated NT Sequence
ttattagaaa gtgaagcaaa actcagacac gccggtacga gaaacagata ccgcacgcat tagaccgcct gaactattat cgatgtcaat cgcgtacttg atagccctct ttagcagtga caaacactga tcgcgagaca tgtcaggctc gtagttgtcg tgtaaaaatg ctgtaatgta tcctgaaccc gatccactag ccacaaagtg tggtactttc aacagggtgc cacccaatgt cacattgtat atctcgggac cgtgttgcat atcataacct cccaatatta taccacagta cagctgctca cggtactcat gtacaatctt gtgtgtcgca gtagccagtg ctttaagagg agggtaaacc atactctcgt cgaatctgta actatattca ctctcagtag gcatttcgct gtcaatctgc atcgcatcgt cactaccaat agcatcatca ccagacgcac gtccgctctt gcgcaattgt agacataaag cctgtgcatg ataacgtact ataacagata gcatctgcga atcggcagct gaaccggaac gtagcataaa aacatttgga agaatgcgag taatcttgcg agccacacga tttacaacaa taggaccaga tgatgtacga ctatccgcag ccaaaagtat accatcacga taaatcatcg cgataatagt cgtacccatg cttatgtcat gagcacgatc gtcagccgcg gtggagccag acacacactc ctgtgcgatt ccggcttccg aaatgccatc ataataacta caatctacca tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMVDCSYYD GISEAGIAQE CVSGSTAADD RAHDISMGTT IIAMIYRDGI LLAADSRTSS GPIVVNRVAR KITRILPNVF MLRSGSAADS QMLSVIVRYH AQALCLQLRK SGRASGDDAI GSDDAMQIDS EMPTESEYSY RFDESMVYPP LKALATATHK IVHEYREQLY CGIILGGYDM QHGPEIYNVT LGGTLLKVPH FVASGSGSGY ITAFLHDNYE PDMSRDQCLS LLKRAIKYAI DIDNSSGGLM RAVSVSRTGV SEFCFTF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGGTAGAT TGTAGTTATT ATGATGGCAT TTCGGAAGCC GGAATCGCAC AGGAGTGTGT GTCTGGCTCC ACCGCGGCTG ACGATCGTGC TCATGACATA AGCATGGGTA CGACTATTAT CGCGATGATT TATCGTGATG GTATACTTTT GGCTGCGGAT AGTCGTACAT CATCTGGTCC TATTGTTGTA AATCGTGTGG CTCGCAAGAT TACTCGCATT CTTCCAAATG TTTTTATGCT ACGTTCCGGT TCAGCTGCCG ATTCGCAGAT GCTATCTGTT ATAGTACGTT ATCATGCACA GGCTTTATGT CTACAATTGC GCAAGAGCGG ACGTGCGTCT GGTGATGATG CTATTGGTAG TGACGATGCG ATGCAGATTG ACAGCGAAAT GCCTACTGAG AGTGAATATA GTTACAGATT CGACGAGAGT ATGGTTTACC CTCCTCTTAA AGCACTGGCT ACTGCGACAC ACAAGATTGT ACATGAGTAC CGTGAGCAGC TGTACTGTGG TATAATATTG GGAGGTTATG ATATGCAACA CGGTCCCGAG ATATACAATG TGACATTGGG TGGCACCCTG TTGAAAGTAC CACACTTTGT GGCTAGTGGA TCGGGTTCAG GATACATTAC AGCATTTTTA CACGACAACT ACGAGCCTGA CATGTCTCGC GATCAGTGTT TGTCACTGCT AAAGAGGGCT ATCAAGTACG CGATTGACAT CGATAATAGT TCAGGCGGTC TAATGCGTGC GGTATCTGTT TCTCGTACCG GCGTGTCTGA GTTTTGCTTC ACTTTCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for BaboA.00409.a.A1.PS01591
PURIFICATION DATe: 8/7/2012
CONCENTRATION: 77.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMVDCSYYD GISEAGIAQE CVSGSTAADD RAHDISMGTT IIAMIYRDGI LLAADSRTSS GPIVVNRVAR KITRILPNVF MLRSGSAADS QMLSVIVRYH AQALCLQLRK SGRASGDDAI GSDDAMQIDS EMPTESEYSY RFDESMVYPP LKALATATHK IVHEYREQLY CGIILGGYDM QHGPEIYNVT LGGTLLKVPH FVASGSGSGY ITAFLHDNYE PDMSRDQCLS LLKRAIKYAI DIDNSSGGLM RAVSVSRTGV SEFCFTF
Validated NT Sequence
ttattagaaa gtgaagcaaa actcagacac gccggtacga gaaacagata ccgcacgcat tagaccgcct gaactattat cgatgtcaat cgcgtacttg atagccctct ttagcagtga caaacactga tcgcgagaca tgtcaggctc gtagttgtcg tgtaaaaatg ctgtaatgta tcctgaaccc gatccactag ccacaaagtg tggtactttc aacagggtgc cacccaatgt cacattgtat atctcgggac cgtgttgcat atcataacct cccaatatta taccacagta cagctgctca cggtactcat gtacaatctt gtgtgtcgca gtagccagtg ctttaagagg agggtaaacc atactctcgt cgaatctgta actatattca ctctcagtag gcatttcgct gtcaatctgc atcgcatcgt cactaccaat agcatcatca ccagacgcac gtccgctctt gcgcaattgt agacataaag cctgtgcatg ataacgtact ataacagata gcatctgcga atcggcagct gaaccggaac gtagcataaa aacatttgga agaatgcgag taatcttgcg agccacacga tttacaacaa taggaccaga tgatgtacga ctatccgcag ccaaaagtat accatcacga taaatcatcg cgataatagt cgtacccatg cttatgtcat gagcacgatc gtcagccgcg gtggagccag acacacactc ctgtgcgatt ccggcttccg aaatgccatc ataataacta caatctacca tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMVDCSYYD GISEAGIAQE CVSGSTAADD RAHDISMGTT IIAMIYRDGI LLAADSRTSS GPIVVNRVAR KITRILPNVF MLRSGSAADS QMLSVIVRYH AQALCLQLRK SGRASGDDAI GSDDAMQIDS EMPTESEYSY RFDESMVYPP LKALATATHK IVHEYREQLY CGIILGGYDM QHGPEIYNVT LGGTLLKVPH FVASGSGSGY ITAFLHDNYE PDMSRDQCLS LLKRAIKYAI DIDNSSGGLM RAVSVSRTGV SEFCFTF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGGTAGAT TGTAGTTATT ATGATGGCAT TTCGGAAGCC GGAATCGCAC AGGAGTGTGT GTCTGGCTCC ACCGCGGCTG ACGATCGTGC TCATGACATA AGCATGGGTA CGACTATTAT CGCGATGATT TATCGTGATG GTATACTTTT GGCTGCGGAT AGTCGTACAT CATCTGGTCC TATTGTTGTA AATCGTGTGG CTCGCAAGAT TACTCGCATT CTTCCAAATG TTTTTATGCT ACGTTCCGGT TCAGCTGCCG ATTCGCAGAT GCTATCTGTT ATAGTACGTT ATCATGCACA GGCTTTATGT CTACAATTGC GCAAGAGCGG ACGTGCGTCT GGTGATGATG CTATTGGTAG TGACGATGCG ATGCAGATTG ACAGCGAAAT GCCTACTGAG AGTGAATATA GTTACAGATT CGACGAGAGT ATGGTTTACC CTCCTCTTAA AGCACTGGCT ACTGCGACAC ACAAGATTGT ACATGAGTAC CGTGAGCAGC TGTACTGTGG TATAATATTG GGAGGTTATG ATATGCAACA CGGTCCCGAG ATATACAATG TGACATTGGG TGGCACCCTG TTGAAAGTAC CACACTTTGT GGCTAGTGGA TCGGGTTCAG GATACATTAC AGCATTTTTA CACGACAACT ACGAGCCTGA CATGTCTCGC GATCAGTGTT TGTCACTGCT AAAGAGGGCT ATCAAGTACG CGATTGACAT CGATAATAGT TCAGGCGGTC TAATGCGTGC GGTATCTGTT TCTCGTACCG GCGTGTCTGA GTTTTGCTTC ACTTTCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA