BaboA.00258.a

seryl-tRNA synthetase, putative

CENTER ID:	BaboA.00258.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.00258.a.A2.GE34094	mature protein		25	536

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_IV001340
OrthoMCL:	OG5_126677
RefSeq:	XP_001609299.1
UniProt:	A7AVA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKVGTIVII VAAVLPFIYI HNAHSISFPV NQGFLNSASR VFRYPNRYND IIEISLHNSY VSPVYSAHTE ERSHPDFTDN DLDKRINEAL KHNFVRRLEN STGYIPTSFD ICVLAAVPEL FHENFSIRHE NHLDTLLNLR KLYLQKVEEE SQLSQLSNRR SDLAKDYLTS GTSQPSIKES SITLRNEIKS LEASIRKLKD DIRSLVNRLP NIVSEDVPKT DTVVQKVYSG TTKAKGQQTT IPHDEVIARF SEFFTSKSTK IAGTGFSAFS GDISRLERAL INFMLDTHHK LFGYKEVSVP FVVNSSTLEA TGHLQRFEND LFKLDDRHQC SGDPGYLIPT GEIPLISLLS NTRLQQNKLP LWLMAYTPCF RAEIQDYGRE TRGLIRNHQF GKVELICVCD ATASTDFHEL MISHIEFILE SLDLPYRRVL LPANELGSTS NKTIDLEVYF PSLRKYIEVS SCSNTLDYQS NKLGILTRSG SKVHCINGSG LAIGRTLSAI LENYQVIKDS GRLEIAIPHI LQPYLEGDQS LAEPLP

NT Sequence

atgagcaagg taggaactat tgtgattatc gtagctgctg tattaccatt tatatacatc cataatgctc attctataag ctttcctgtt aatcaagggt ttttgaatag cgcttcacga gtttttagat atcctaatag atataacgac atcatagaaa ttagcttaca taattcgtat gtatctcctg tctattcggc acatactgag gaacgatctc acccagattt cactgacaac gatttggata agcgtataaa cgaagcacta aaacataatt ttgtaagacg ccttgagaat tctacaggct acattcctac gagctttgat atatgtgttt tggcagctgt accagagtta ttccatgaaa atttcagcat acgtcacgaa aaccatttgg acactcttct aaatctacgt aaattgtatc tacagaaagt tgaagaggag agtcagttgt cacaactgtc taatcgtcgt tctgatttgg caaaggatta tcttacgtct ggcacctccc agccgtcgat caaagaatct tctataaccc tgcgcaatga gataaaatct ctagaggcaa gtataaggaa attgaaagat gacattaggt ccctagtgaa cagattgcca aacatagtgt cagaggatgt acccaagacc gacaccgtag tgcagaaagt atatagcggt actacgaagg ctaagggaca acaaaccaca atcccacatg atgaggttat tgcgcgcttc agtgaattct ttacttcaaa atcaactaaa attgcaggta cagggttttc tgcattcagt ggagatataa gccgattaga aagagcactg attaatttca tgcttgatac acatcacaag ctatttggtt acaaagaagt atccgtgcca tttgttgtca acagctccac tttggaagct acaggccacc tacaacgatt tgaaaacgat cttttcaaac tggacgatcg tcaccaatgt agcggcgatc ccggttactt gatcccaaca ggagaaatac ctcttatatc acttttgagt aacacaaggc tacaacaaaa taaattgcct ttatggttaa tggcatacac tccatgcttc agagcggaaa tacaggatta cggtagagaa acacgtggac ttattcgaaa ccaccaattt ggcaaagttg aattgatctg cgtatgtgat gccactgcgt caacggattt ccatgaacta atgatatcac atatagaatt catattggag tctttagatt tgccctaccg cagggtacta ttgccagcta acgaactggg ttcaacgtca aacaaaacaa ttgaccttga agtatacttc ccttctctaa ggaaatacat agaagtctcc agttgcagta acaccttgga ctaccaatct aataagctgg gcatattaac gcgatcaggc tcaaaggtgc actgtattaa cggttctgga cttgcaatag gacgtacact ttcagcaata ttggagaact accaagttat aaaggatagt ggacgtctgg agattgccat tccacatata cttcaaccat atctggaagg cgatcagtct ttagcagaac cattgccatg a

Details for BaboA.00258.a.A2.GE34094

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/5/2012
EXPECTED MW:	60kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	61

Validated AA Sequence

Validated NT Sequence

annnnnnttn nnnnntttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg tctataagct ttcctgttaa tcaagggttt ttgaatagcg cttcacgagt ttttagatat cctaatagat ataacgacat catagaaatt agcttacata attcgtatgt atctcctgtc tattcggcac atactgagga acgatctcac ccagatttca ctgacaacga tttggataag cgtataaacg aagcactaaa acataatttt gtaagacgcc ttgagaattc tacaggctac attcctacga gctttgatat atgtgttttg gcagctgtac cagagttatt ccatgaaaat ttcagcatac gtcacgaaaa ccatttggac actcttctaa atctacgtaa attgtatcta cagaaagttg aagaggagag tcagttgtca caactgtcta atcgtcgttc tgatttggca aaggattatc ttacgtctgg cacctcccag ccgtcgatca aagaatcttc tataaccctg cgcaatgaga taaaatctct agaggcaagt ataaggaaat tgaaagatga cattaggtcc ctagtgaaca gattgccaaa catagtgtca gaggatgtac ccaagaccga caccgtagtg cagaaagtat atagcggtac tacgaaggct aagggacaac aaaccacaat cccacatgat gaggttattg cgcgcttcag tgaattcttt acttcaaaat caactaaaat tgcaggtaca gggttttctg cattcagtgg agatataagc cgantagaaa gagcactgat taatttcatg cttgatacac atcacaagct atttggttac aaagaagtat ccgtgccatt tgttgtcaac agctccnctt tggaagctac nggcnnccta caacgatttg aaacgatctt tcaaactggn cgatcgtcan cnatgtagcg ncnatccnng ntantnatnc nanngnanaa nncnntnnnt nanttttnag tannnnnnta nannaaanna nnnnntnnng gtnnnnntan nnncnngctn naannnnant ngnnnnnnaa nnnnnnntnn nnnnannnnn nnnnnnnnan nnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSISFPVNQ GFLNSASRVF RYPNRYNDII EISLHNSYVS PVYSAHTEER SHPDFTDNDL DKRINEALKH NFVRRLENST GYIPTSFDIC VLAAVPELFH ENFSIRHENH LDTLLNLRKL YLQKVEEESQ LSQLSNRRSD LAKDYLTSGT SQPSIKESSI TLRNEIKSLE ASIRKLKDDI RSLVNRLPNI VSEDVPKTDT VVQKVYSGTT KAKGQQTTIP HDEVIARFSE FFTSKSTKIA GTGFSAFSGD ISRLERALIN FMLDTHHKLF GYKEVSVPFV VNSSTLEATG HLQRFENDLF KLDDRHQCSG DPGYLIPTGE IPLISLLSNT RLQQNKLPLW LMAYTPCFRA EIQDYGRETR GLIRNHQFGK VELICVCDAT ASTDFHELMI SHIEFILESL DLPYRRVLLP ANELGSTSNK TIDLEVYFPS LRKYIEVSSC SNTLDYQSNK LGILTRSGSK VHCINGSGLA IGRTLSAILE NYQVIKDSGR LEIAIPHILQ PYLEGDQSLA EPLP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctataagc tttcctgtta atcaagggtt tttgaatagc gcttcacgag tttttagata tcctaataga tataacgaca tcatagaaat tagcttacat aattcgtatg tatctcctgt ctattcggca catactgagg aacgatctca cccagatttc actgacaacg atttggataa gcgtataaac gaagcactaa aacataattt tgtaagacgc cttgagaatt ctacaggcta cattcctacg agctttgata tatgtgtttt ggcagctgta ccagagttat tccatgaaaa tttcagcata cgtcacgaaa accatttgga cactcttcta aatctacgta aattgtatct acagaaagtt gaagaggaga gtcagttgtc acaactgtct aatcgtcgtt ctgatttggc aaaggattat cttacgtctg gcacctccca gccgtcgatc aaagaatctt ctataaccct gcgcaatgag ataaaatctc tagaggcaag tataaggaaa ttgaaagatg acattaggtc cctagtgaac agattgccaa acatagtgtc agaggatgta cccaagaccg acaccgtagt gcagaaagta tatagcggta ctacgaaggc taagggacaa caaaccacaa tcccacatga tgaggttatt gcgcgcttca gtgaattctt tacttcaaaa tcaactaaaa ttgcaggtac agggttttct gcattcagtg gagatataag ccgattagaa agagcactga ttaatttcat gcttgataca catcacaagc tatttggtta caaagaagta tccgtgccat ttgttgtcaa cagctccact ttggaagcta caggccacct acaacgattt gaaaacgatc ttttcaaact ggacgatcgt caccaatgta gcggcgatcc cggttacttg atcccaacag gagaaatacc tcttatatca cttttgagta acacaaggct acaacaaaat aaattgcctt tatggttaat ggcatacact ccatgcttca gagcggaaat acaggattac ggtagagaaa cacgtggact tattcgaaac caccaatttg gcaaagttga attgatctgc gtatgtgatg ccactgcgtc aacggatttc catgaactaa tgatatcaca tatagaattc atattggagt ctttagattt gccctaccgc agggtactat tgccagctaa cgaactgggt tcaacgtcaa acaaaacaat tgaccttgaa gtatacttcc cttctctaag gaaatacata gaagtctcca gttgcagtaa caccttggac taccaatcta ataagctggg catattaacg cgatcaggct caaaggtgca ctgtattaac ggttctggac ttgcaatagg acgtacactt tcagcaatat tggagaacta ccaagttata aaggatagtg gacgtctgga gattgccatt ccacatatac ttcaaccata tctggaaggc gatcagtctt tagcagaacc attgccataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa