BaboA.00161.a

adenosine deaminase, putative

CENTER ID:	BaboA.00161.a
ORGANISM:	Babesia bovis T2Bo
ASSOCIATED DISEASE:	Babesiosis (atypical)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaboA.00161.a.A1.GE34096	full length		1	362

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	PiroplasmaDB:BBOV_I001470
OrthoMCL:	OG5_127245
RefSeq:	XP_001608799.1
UniProt:	A7AW03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKTMDVYPT NAELQKMGEW ERKSIWKKMP KMELHMHGDI ACPVNKLIDI YNDYADENDR LTEQDILQNI ICNYKIKDLV EFIDKTRMIC KVFANNSKIL ERSSKEAILA KYREGVVGLE FRFSPAYLAE GCGYSYDEVV NDVLSGMEQA KKQIKTPFEV GAIYIGEIGI HDQFPFSESV DYFFKHRDKF IGFDNAGYPA DFAPFADQFK RLVDAGVNLT LHAGETPPDC NERLAMALDF GAKRIGHGIE CAKSPEMMKR LIDEDVILEV CPKSNWITNP SINMSDHPIR KIYDAGVKVC INTDDPMMMT NSLHEEYDLL FTHLGFGYAD FVKMNTWALE KSFLSKEAKL TLKKAYFPNA NI

NT Sequence

atgttgaaaa caatggatgt atatcccact aacgccgaac tccagaagat gggagaatgg gagcggaaat ctatatggaa gaagatgcca aagatggagc ttcatatgca tggagacatt gcatgccctg ttaataagtt aattgacata tataacgatt atgcggatga aaatgatcgt ctaaccgaac aagatatctt gcaaaatatt atatgtaatt acaaaattaa ggatttagtg gaattcatcg ataagacacg tatgatatgt aaagtttttg caaacaattc aaaaatcctc gaacgctcat ctaaggaagc tattcttgct aaatacaggg aaggtgtagt gggattggag ttcaggtttt ccccggctta ccttgccgaa ggatgtggtt attcgtatga tgaggtggtt aatgatgttc tgtctggtat ggagcaagct aaaaaacaaa taaagacacc attcgaggtt ggagcaatat atattggtga gataggtatc catgatcagt tcccgttttc agaatcagtt gattactttt tcaagcatcg tgacaaattt attggttttg acaatgcggg atatcctgcc gactttgcac catttgcgga tcaattcaag cgtcttgtag atgccggcgt taatctcact cttcatgcag gagaaacgcc acctgattgt aatgaacgtt tggcaatggc tcttgacttt ggtgccaaac gtataggtca tggtattgaa tgtgccaaat cacccgaaat gatgaagagg ttgatagatg aggatgttat actagaggtt tgtccaaagt cgaattggat taccaaccca tcaattaata tgtcggatca cccaattagg aagatatatg atgctggtgt taaggtgtgt atcaataccg atgatcctat gatgatgacc aacagcctac acgaggaata tgatctgctg ttcacccatt taggatttgg atatgcagat ttcgttaaga tgaatacatg ggcattagaa aagtcattcc ttagcaaaga ggcaaaattg accctcaaga aagcatactt ccctaacgca aacatataa

Details for BaboA.00161.a.A1.GE34096

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	43kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttgcgttagg gaagtatgct ttcttgaggg tcaattttgc ctctttgcta aggaatgact tttctaatgc ccatgtattc atcttaacga aatctgcata tccaaatcct aaatgggtga acagcagatc atattcctcg tgtaggctgt tggtcatcat cataggatca tcggtattga tacacacctt aacaccagca tcatatatct tcctaattgg gtgatccgac atattaattg atgggttggt aatccaattc gactttggac aaacctctag tataacatcc tcatctatca acctcttcat catttcgggt gatttggcac attcaatacc atgacctata cgtttggcac caaagtcaag agccattgcc aaacgttcat tacaatcagg tggcgtttct cctgcatgaa gagtgagatt aacgccggca tctacaagac gcttgaattg atccgcaaat ggtgcaaagt cggcaggata tcccgcattg tcaaaaccaa taaatttgtc acgatgcttg aaaaagtaat caactgattc tgaaaacggg aactgatcat ggatacctat ctcaccaata tatattgctc caacctcgaa tggtgtcttt atttgttttt tagcttgctc cataccagac agaacatcat taaccacctc atcatacgaa taaccacatc cttcggcaag gtaagccggg gaaaacctga actccaatcc cactacacct tccctgtatt tagcaagaat agcttcctta gatgagcgtt cgaggatttt tgaattgttt gcaaaaactt tacatatcat acgtgtctta tcgatgaatt ccactaaatc cttaattttg taattacata taatattttg caagatatct tgttcggtta gacgatcatt ttcatccgca taatcgttat atatgtcaat taacttatta acagggcatg caatgtctcc atgcatatga agctccatct ttggcatctt cttccatata gatttccgct cccattctcc catcttctgg agttcggcgt tagtgggata tacatccatt gttttcaaca tcgaaccagg accctgggtc tgagcttcca gggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLKTMDVYP TNAELQKMGE WERKSIWKKM PKMELHMHGD IACPVNKLID IYNDYADEND RLTEQDILQN IICNYKIKDL VEFIDKTRMI CKVFANNSKI LERSSKEAIL AKYREGVVGL EFRFSPAYLA EGCGYSYDEV VNDVLSGMEQ AKKQIKTPFE VGAIYIGEIG IHDQFPFSES VDYFFKHRDK FIGFDNAGYP ADFAPFADQF KRLVDAGVNL TLHAGETPPD CNERLAMALD FGAKRIGHGI ECAKSPEMMK RLIDEDVILE VCPKSNWITN PSINMSDHPI RKIYDAGVKV CINTDDPMMM TNSLHEEYDL LFTHLGFGYA DFVKMNTWAL EKSFLSKEAK LTLKKAYFPN ANI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaaaaca atggatgtat atcccactaa cgccgaactc cagaagatgg gagaatggga gcggaaatct atatggaaga agatgccaaa gatggagctt catatgcatg gagacattgc atgccctgtt aataagttaa ttgacatata taacgattat gcggatgaaa atgatcgtct aaccgaacaa gatatcttgc aaaatattat atgtaattac aaaattaagg atttagtgga attcatcgat aagacacgta tgatatgtaa agtttttgca aacaattcaa aaatcctcga acgctcatct aaggaagcta ttcttgctaa atacagggaa ggtgtagtgg gattggagtt caggttttcc ccggcttacc ttgccgaagg atgtggttat tcgtatgatg aggtggttaa tgatgttctg tctggtatgg agcaagctaa aaaacaaata aagacaccat tcgaggttgg agcaatatat attggtgaga taggtatcca tgatcagttc ccgttttcag aatcagttga ttactttttc aagcatcgtg acaaatttat tggttttgac aatgcgggat atcctgccga ctttgcacca tttgcggatc aattcaagcg tcttgtagat gccggcgtta atctcactct tcatgcagga gaaacgccac ctgattgtaa tgaacgtttg gcaatggctc ttgactttgg tgccaaacgt ataggtcatg gtattgaatg tgccaaatca cccgaaatga tgaagaggtt gatagatgag gatgttatac tagaggtttg tccaaagtcg aattggatta ccaacccatc aattaatatg tcggatcacc caattaggaa gatatatgat gctggtgtta aggtgtgtat caataccgat gatcctatga tgatgaccaa cagcctacac gaggaatatg atctgctgtt cacccattta ggatttggat atgcagattt cgttaagatg aatacatggg cattagaaaa gtcattcctt agcaaagagg caaaattgac cctcaagaaa gcatacttcc ctaacgcaaa catataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa