BabiA.18461.a

2-octaprenyl-6-methoxyphenol hydroxylase (Trw type IV secretion machinery)

CENTER ID: BabiA.18461.a
ORGANISM: Bartonella birtlesii
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BabiA.18461.a.B2.GE35920 soluble domain 43 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128116
PATRIC ID: fig|1094552.3.peg.1064
UniProt: D0AAX8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYSDQDKNIK PNKKAKSNKT AKQCDLLIAG GAIVGLTLAV SLKQAAPELK INIVDAAPQE NIPVSNIRTI ALAAASLRML KQLQCWEHIK SSCQPIHSMI ITDACTNDPV KPTLLTFEGE ITPDEPFATM VEHRELIKAL KKRVKELAIP VITNVRVIDF HQEEQQTTVT LSNAEIWQTK LLIAADGAHS KLREKAGLKN FSHPYKQTAI ICTIEHEKPH HGQAIQHFFP AGPFALLPLK GNRSAIVWNE SHKIAQYYLK ADTLIFETEL EKRIGHKLGK LSWNGERQAF PLQLSLTHQC IKPRFALIGD AAHTIHPLAG QGLNLGLRDS AALAEVIIET ARLGLDIGSL TALERYQSWR RFEVVRMAFS NDWLNKLFSN DNLFLRMIRD TGLGIVNKIP KIKKYFIQEA SGLTPHAPRL LQGLPL
NT Sequence
gtgtattctg accaagataa aaatataaaa cccaataaaa aagcaaaatc caataaaacg gcaaaacaat gcgatcttct cattgcgggg ggagcaattg tcggtttaac gctggcggta tcgctcaaac aagcagcccc tgaactaaaa atcaatatcg ttgatgctgc tcctcaagaa aatatccctg tttccaatat acggaccatc gcccttgctg ctgcttctct tcgtatgtta aaacaattgc aatgttggga acatataaaa tcttcttgcc aacccattca ctcaatgatc attaccgatg catgcacaaa tgatcccgtc aaaccaactc ttttaacctt tgaaggagag attactcctg atgagccttt cgctactatg gtagaacaca gagaactcat caaagctcta aaaaaacgtg taaaagaact cgctatccct gtcattacaa acgtgcgtgt cattgatttt caccaagaag agcaacaaac aaccgttact ttaagcaatg cagaaatttg gcaaaccaaa ttgcttattg cagctgatgg tgcccattcg aaattacgtg aaaaagcggg gcttaaaaat ttttctcacc cctataaaca aacagccatc atctgcacca tcgaacatga aaaacctcat catggacaag ccattcaaca ttttttccct gcaggtcctt tcgcacttct ccctctcaaa ggtaaccgat ccgctattgt atggaatgaa tcccataaaa tcgcacaata ttacctcaaa gccgatactc ttatttttga aacagaactc gaaaaacgca ttggccacaa acttgggaag ctttcttgga atggtgaacg tcaagctttt cctttgcagc tctctttaac acaccaatgc attaagcctc gttttgctct cattggtgat gctgcccaca caatccatcc cctcgcagga caagggctca atttaggatt acgcgacagc gccgcgctcg ccgaagtaat tattgaaaca gcacgattgg ggcttgatat tggctccctc accgctttag agcgctatca aagctggaga cgttttgaag ttgttcgtat ggctttcagc aatgactggc tcaacaaact cttttctaac gataatcttt tcttacgcat gatacgcgat accggtcttg gaattgttaa taaaatacca aaaataaaga aatattttat tcaagaagct tctggtctta cccctcatgc cccacgtcta ctacaaggac tccctcttta a
Details for BabiA.18461.a.B2.GE35920
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHLMK QAAPELKINI VDAAPQENIP VSNIRTIALA AASLRMLKQL QCWEHIKSSC QPIHSMIITD ACTNDPVKPT LLTFEGEITP DEPFATMVEH RELIKALKKR VKELAIPVIT NVRVIDFHQE EQQTTVTLSN AEIWQTKLLI AADGAHSKLR EKAGLKNFSH PYKQTAIICT IEHEKPHHGQ AIQHFFPAGP FALLPLKGNR SAIVWNESHK IAQYYLKADT LIFETELEKR IGHKLGKLSW NGERQAFPLQ LSLTHQCIKP RFALIGDAAH TIHPLAGQGL NLGLRDSAAL AEVIIETARL GLDIGSLTAL XRYQSWXRFX XXRMXFSXDW XXXXXXTXXF XXXXTXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
attttgtnnn nnttaagaag gagatatacc atggctcacc accaccacca ccttatgaaa caagcagccc ctgaactaaa aatcaatatc gttgatgctg ctcctcaaga aaatatccct gtttccaata tacggaccat cgcccttgct gctgcttctc ttcgtatgtt aaaacaattg caatgttggg aacatataaa atcttcttgc caacccattc actcaatgat cattaccgat gcatgcacaa atgatcccgt caaaccaact cttttaacct ttgaaggaga gattactcct gatgagcctt tcgctactat ggtagaacac agagaactca tcaaagctct aaaaaaacgt gtaaaagaac tcgctatccc tgtcattaca aacgtgcgtg tcattgattt tcaccaagaa gagcaacaaa caaccgttac tttaagcaat gcagaaattt ggcaaaccaa attgcttatt gcagctgatg gtgcccattc gaaattacgt gaaaaagcgg ggcttaaaaa tttttctcac ccctataaac aaacagccat catctgcacc atcgaacatg aaaaacctca tcatggacaa gccattcaac attttttccc tgcaggtcct ttcgcacttc tccctctcaa aggtaaccga tccgctattg tatggaatga atcccataaa atcgcacaat attacctcaa agccgatact cttatttttg aaacagaact cgaaaaacgc attggccaca aacttgggaa gctttcttgg aatggtgaac gtcaagcttt tcctttgcag ctctctttaa cacaccaatg cattaagcct cgttttgctc tcattggtga tgctgcccac acaatccatc ccctcgcagg acaagggctc aatttaggat tacgcgacag cgccgcgctc gccgaagtaa ttattgaaac agcacgattg gggctngata ttggctccct caccgcttta nancgctatc aaagctggng acgttttgan nttnnncgta tggntttcag cnntgactgg nnnnnnnntc ntttnntnac gannnnnttt nctnnncang nnacncnata nnnnnnnnnn nnnnnnaann nnnaaaanna nannnntttn tnnnnnnnnn nnnnnnnnnc nnnnnnnnnc cnnannnnnn nnnnnnnnnn nnnnnnnnnn nnnnc
Expected Protein Sequence
MAHHHHHHMK QAAPELKINI VDAAPQENIP VSNIRTIALA AASLRMLKQL QCWEHIKSSC QPIHSMIITD ACTNDPVKPT LLTFEGEITP DEPFATMVEH RELIKALKKR VKELAIPVIT NVRVIDFHQE EQQTTVTLSN AEIWQTKLLI AADGAHSKLR EKAGLKNFSH PYKQTAIICT IEHEKPHHGQ AIQHFFPAGP FALLPLKGNR SAIVWNESHK IAQYYLKADT LIFETELEKR IGHKLGKLSW NGERQAFPLQ LSLTHQCIKP RFALIGDAAH TIHPLAGQGL NLGLRDSAAL AEVIIETARL GLDIGSLTAL ERYQSWRRFE VVRMAFSNDW LNKLFSNDNL FLRMIRDTGL GIVNKIPKIK KYFIQEASGL TPHAPRLLQG LPL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac aagcagcccc tgaactaaaa atcaatatcg ttgatgctgc tcctcaagaa aatatccctg tttccaatat acggaccatc gcccttgctg ctgcttctct tcgtatgtta aaacaattgc aatgttggga acatataaaa tcttcttgcc aacccattca ctcaatgatc attaccgatg catgcacaaa tgatcccgtc aaaccaactc ttttaacctt tgaaggagag attactcctg atgagccttt cgctactatg gtagaacaca gagaactcat caaagctcta aaaaaacgtg taaaagaact cgctatccct gtcattacaa acgtgcgtgt cattgatttt caccaagaag agcaacaaac aaccgttact ttaagcaatg cagaaatttg gcaaaccaaa ttgcttattg cagctgatgg tgcccattcg aaattacgtg aaaaagcggg gcttaaaaat ttttctcacc cctataaaca aacagccatc atctgcacca tcgaacatga aaaacctcat catggacaag ccattcaaca ttttttccct gcaggtcctt tcgcacttct ccctctcaaa ggtaaccgat ccgctattgt atggaatgaa tcccataaaa tcgcacaata ttacctcaaa gccgatactc ttatttttga aacagaactc gaaaaacgca ttggccacaa acttgggaag ctttcttgga atggtgaacg tcaagctttt cctttgcagc tctctttaac acaccaatgc attaagcctc gttttgctct cattggtgat gctgcccaca caatccatcc cctcgcagga caagggctca atttaggatt acgcgacagc gccgcgctcg ccgaagtaat tattgaaaca gcacgattgg ggcttgatat tggctccctc accgctttag agcgctatca aagctggaga cgttttgaag ttgttcgtat ggctttcagc aatgactggc tcaacaaact cttttctaac gataatcttt tcttacgcat gatacgcgat accggtcttg gaattgttaa taaaatacca aaaataaaga aatattttat tcaagaagct tctggtctta cccctcatgc cccacgtcta ctacaaggac tccctctttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat