SSGCID: Target Page

BabiA.18409.a

TrwN component of type IV secretion system (Trw type IV secretion machinery)

CENTER ID:	BabiA.18409.a
ORGANISM:	Bartonella birtlesii
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BabiA.18409.a.B2.GE35916	mature protein		27	217

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	D0AAY0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLMIISNFI MFSALCTPIQ QSTAFSAVVV KESSANVCAI NDNGLLQQPS LFGKTVVKNE QYTQNGHNFD VYWEKNNNEN LKWFYSFFPT LLAPYRDFKV VQTALKHCCE QISPHYSIRQ AVLNFHNIEN FKNGFANADV QKISSHIEMK TSTFVDKESQ KLMKLQRENE EQIIELESLS LFLEEDAFTH KASGVHDAFK TENSSLVEPS LEEQQEQ

NT Sequence

gtgcctctta tgattatttc aaatttcata atgttttctg ctctatgcac accaatccaa caatctacag cattttcggc tgtggtcgta aaggaatctt cggcaaacgt ctgtgcaata aatgataatg gattattaca gcagccctct ttgtttggaa aaacagttgt gaaaaatgaa cagtatacac agaatggaca taattttgac gtatattggg aaaaaaataa caatgaaaat ctgaaatggt tttattcttt ttttcctact ttacttgccc cttataggga ctttaaggtt gtgcagactg ctttaaagca ttgttgtgag caaataagcc cacactacag tattagacag gctgtactca attttcataa tatagaaaac tttaaaaatg gttttgccaa tgctgatgta cagaaaatct cttcacacat cgaaatgaaa acttctacgt ttgtagataa agaatcacaa aaacttatga aacttcaaag agaaaatgaa gaacagataa ttgaactgga atctctgtct cttttcttag aagaagatgc ttttacacat aaagcaagtg gtgttcatga tgcttttaaa actgagaatt cttcattggt agagccttca ttggaagaac aacaggaaca ataa

Details for BabiA.18409.a.B2.GE35916

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA VVVKESSANV CAINDNGLLQ QPSLFGKTVV KNEQYTQNGH NFDVYWEKNN NENLKWFYSF FPTLLAPYRD FKVVQTALKH CCEQISPHYS IRQAVLNFHN IENFKNGFAN ADVQKISSHI EMKTSTFVDK ESQKLMKLQR ENEEQIIELE SLSLFLEEDA FTHKASGVHD AFKTENSSLV EPSLEEQQEQ

Validated NT Sequence

ttgtttactt taagaaggag anataccatg gctcaccacc accaccacca tatggctgtg gtcgtaaagg aatcttcggc aaacgtctgt gcaataaatg ataatggatt attacagcag ccctctttgt ttggaaaaac agttgtgaaa aatgaacagt atacacagaa tggacataat tttgacgtat attgggaaaa aaataacaat gaaaatctga aatggtttta ttcttttttt cctactttac ttgcccctta tagggacttt aaggttgtgc agactgcttt aaagcattgt tgtgagcaaa taagcccaca ctacagtatt agacaggctg tactcaattt tcataatata gaaaacttta aaaatggttt tgccaatgct gatgtacaga aaatctcttc acacatcgaa atgaaaactt ctacgtttgt agataaagaa tcacaaaaac ttatgaaact tcaaagagaa aatgaagaac agataattga actggaatct ctgtctcttt tcttagaaga agatgctttt acacataaag caagtggtgt tcatgatgct tttaaaactg agaattcttc attggtagag ccttcattgg aagaacaaca ggaacaataa gtgagtaaga taggatccgg ctgcnnnnnn cccccnnnnn nnnnnnnnnn nnttngntgn ntgcnnnctn tgnnnnnnnt nnntnnanng nnnncgnnct ncnncnnntc ctgtcnnncn nnnnnngnnn nnccannnnn nnncnnnnnn nnnnnncnnn nnncnnnnnn agnaganacn actcncnnnn nnnnnnnnnn nnnannnnnn ngnnnannnt acgccatcnn nnnnnnnttn ccnanncnnn gngannngnn nnnnnctntn nanntnnnat nttccnnnnn nnnnnnntnn nnnnnca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg tggtcgtaaa ggaatcttcg gcaaacgtct gtgcaataaa tgataatgga ttattacagc agccctcttt gtttggaaaa acagttgtga aaaatgaaca gtatacacag aatggacata attttgacgt atattgggaa aaaaataaca atgaaaatct gaaatggttt tattcttttt ttcctacttt acttgcccct tatagggact ttaaggttgt gcagactgct ttaaagcatt gttgtgagca aataagccca cactacagta ttagacaggc tgtactcaat tttcataata tagaaaactt taaaaatggt tttgccaatg ctgatgtaca gaaaatctct tcacacatcg aaatgaaaac ttctacgttt gtagataaag aatcacaaaa acttatgaaa cttcaaagag aaaatgaaga acagataatt gaactggaat ctctgtctct tttcttagaa gaagatgctt ttacacataa agcaagtggt gttcatgatg cttttaaaac tgagaattct tcattggtag agccttcatt ggaagaacaa caggaacaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat