SSGCID: Target Page

BabiA.18408.a

TrwK component of type IV secretion system (Trw type IV secretion machinery)

CENTER ID:	BabiA.18408.a
ORGANISM:	Bartonella birtlesii
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BabiA.18408.a.B1.GE35915	full length		1	823

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1094552.3.peg.1076
UniProt:	D0AAY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMAVENSKRL ASETPVSLFI PYSHHITDTI ISTKNAEYLS VWKIDGRSHQ SASEEDIFQW TKELNNTLRG IASANLSLWT HIVRRRVYEY PDSAFDQMFC YQLDEKYRQS FTGYNLMVND LYLTVIFQPI ADKVMSFFSQ HERETLDQKK MRQESCIKAL NDINNTLAQS LKRYGAELLG AYEKNGHAYS SALEFLGMLI NGEYRPMPIC YERFADYMSI NRPFFSKWGA LGELRTSTGM RRFGMLEIKE YDTTTKPGQL NALLESDFEF VLTQSFSVLS RHAAKDYLQR HQRNLIDARD VATSQIEQID EALNQLVSGH FVMGEHHCTL TIYGETVQQV RDYMAKANAI LLDAAVLPKP VDLAIEAGYW AQLPANWKWR PRPAPITSLN FLSFSSFHNF MSGKPTGNPW GPAVTILKTN SKTPLYFNFH ASKIEEDSTD KRLLGNTALI GQSGSGKTVL LGFLLAQAQK FKPTTVVFDK DRGMEIIIRA MGGRYLTFKP GKQSGFNPFQ LPPTQDNLIF LKQFVKKLAE AGGEVTHHDE EEINQAVMSV MSSNIDRSLR RLSFLVQFLP NPHLNDYNAH PSVHARLIKW CEGGDYGWLF DNPTDALDLS THQIYGFDIT EFLDNSETRT PVMMYLLYRT EGMISGQRFM YIFDEFWKPL QDPYFEDLAK NKQKTIRKQN GIFVYATQEP SDALESNIAK TLIQQCATYI FLANPKANYE DYTQGFKLTD TEFELVKGLG EFSRQFLIKQ GDQSALAELN LGKFHMTIDG KTKECDFSEE LLVLSGTPDN AELAESIITK VGDDPAIWLP VFLQHIKTDR RET

NT Sequence

atgatggctg ttgaaaacag caaacgtctt gcctcagaga cacctgtaag tctgttcata ccctattcgc accatattac agatacaatt atctctacaa aaaatgcgga atatctatcg gtttggaaaa ttgatggacg ttcgcatcaa agtgcgtcag aggaagatat ttttcagtgg accaaagagc taaataatac cctacgtggt attgcatcag ccaatttatc attgtggaca catatcgtac gccgccgtgt ctatgaatat cctgattcag catttgacca aatgttttgc tatcagcttg atgaaaagta tcgacaaagt ttcactggtt ataatcttat ggtgaatgac ctgtatttga ctgttatttt ccaaccgata gcagataaag ttatgtcatt cttttctcag catgaacgtg aaacgctcga tcaaaaaaag atgcggcagg aatcatgtat caaagccctc aacgacatta acaacacttt agctcaatcg ttaaaacggt acggtgcaga gcttcttggt gcttatgaaa aaaatggcca tgcctattct tctgcgcttg aattccttgg aatgcttatt aacggcgaat ataggcctat gccaatctgt tatgagcgtt ttgccgacta tatgtctatt aatcggccat ttttctcaaa atggggtgct cttggagaat tacgcacaag cactggaatg cgtcgatttg gaatgctgga aattaaggaa tacgacacaa caaccaaacc aggtcaatta aatgctttgc tggaaagtga tttcgaattt gtcttgacac aaagcttttc agtactttcg cgacatgctg ctaaggatta tttgcaacgc caccaacgta atctgatcga tgctcgcgat gtggcaacaa gccaaattga gcaaattgat gaagcattaa accagcttgt cagtggacac tttgttatgg gtgaacatca ctgcacattg accatttatg gtgagacagt tcaacaagtc cgtgactata tggcaaaagc aaatgcaata ttgctagatg ctgcagtatt gccaaaacct gtagatttgg ctatagaagc tggttattgg gcacaactgc ccgcaaattg gaaatggcgg ccgcgtccag caccaatcac gtcactaaac tttttatcat tttcatcttt tcataatttt atgtcaggca aaccaactgg aaatccatgg gggccggcag tcacaattct gaaaacaaac agcaaaacac cgctttattt taattttcat gcttctaaaa ttgaggaaga ttcaacagat aaacgcttac ttggcaatac cgcccttatc gggcaatctg gttccggtaa aacagtgcta cttggttttc tgttagcgca agcacaaaag tttaaaccaa caaccgttgt ttttgataaa gatcgcggca tggaaattat tattagagct atgggtggtc gatatctaac attcaagcct ggtaagcaaa gtggtttcaa tccttttcaa cttcctccaa cacaagataa cctgattttt ctaaaacagt ttgttaaaaa attagctgaa gctggtggtg aagttacaca tcacgatgaa gaagaaatta accaagctgt catgtctgtc atgagcagca acattgacag atcgctgcgt cgtttgtctt ttcttgtaca attcttacca aatccacatt taaatgacta taatgctcac ccatctgttc atgctcgttt gataaaatgg tgcgaaggtg gtgattatgg gtggttattt gataacccta ctgatgcact cgatctttca acacaccaaa tttacggttt tgatattact gagttcttag ataactcaga aacgcgtaca ccagtcatga tgtatctgct ttaccgcaca gaaggaatga ttagcggaca gcgatttatg tatatttttg atgagttttg gaaacctttg caagatccat attttgaaga tctagcaaaa aataagcaaa aaactatccg taaacaaaat ggtatttttg tttacgctac acaagaaccc agtgatgcct tggaaagcaa tatagccaag acacttattc aacaatgtgc aacctacatt ttcttggcca atcccaaagc aaattacgaa gattatacac aaggttttaa actaacagat actgaatttg aactggttaa aggccttggt gaatttagtc gccagtttct catcaagcaa ggcgaccaat ctgcacttgc agagttaaat ctcggcaaat ttcatatgac aatcgatgga aaaactaaag aatgcgactt tagtgaagag cttttggtat tatcaggtac acctgataat gcagaattgg ctgaaagcat cattactaaa gtgggtgatg atccagcaat atggttacca gtttttttac aacatataaa aactgacagg agggaaacat ga

Details for BabiA.18408.a.B1.GE35915

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	95kDa
OBSERVED MW:	95kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	81
PERCENT COVERAGE:	43

Validated AA Sequence

Validated NT Sequence

tttgtttact ttaagaagga ganataccat ggctcaccac caccaccacc atatgatggc tgttgaaaac agcaaacgtc ttgcctcaga gacacctgta agtctgttca taccctattc gcaccatatt acagatacaa ttatctctac aaaaaatgcg gaatatctat cggtttggaa aattgatgga cgttcgcatc aaagtgcgtc agaggaagat atttttcagt ggaccaaaga gctaaataat accctacgtg gtattgcatc agccaattta tcattgtgga cacatatcgt acgccgccgt gtctatgaat atcctgattc agcatttgac caaatgtttt gctatcagct tgatgaaaag tatcgacaaa gtttcactgg ttataatctt atggtgaatg acctgtattt gactgttatt ttccaaccga tagcagataa agttatgtca ttcttttctc agcatgaacg tgaaacgctc gatcaaaaaa agatgcggca ggaatcatgt atcaaagccc tcaacgacat taacaacact ttagctcaat cgttaaaacg gtacggtgca gagcttcttg gtgcttatga aaaaaatggc catgcctatt cttctgcgct tgaattcctt ggaatgctta ttaacggcga atataggcct atgccaatct gttatgagcg ttttgccgac tatatgtcta ttaatcggcc atttttctca aaatggggtg ctcttggaga attacgcaca agcactggaa tgcgtcnatt tggaatgctg gaaattaagg aatacgacac aacaaccaaa ccangtcaat taaatgcttt gctggaaagt gatttcgaan ttgtctngac acaaagcttt tcagtacttt cgcgacatgc tgcnannnnn atttncancn nnnnnngtan tcngancgat gctcgcnann nnnnacnnnn nnnnnnnnnn nntnatgaca ntnannnctn ncnnngnnnn nnntnngggn naanntnnnn nnnntnncnn tttnngnnnn nnnnnnnnna nnnnnnnnnn nnnnnnnatn nnnannnngn nntnnnnann ccnnnannnn nangg

Expected Protein Sequence

MAHHHHHHMM AVENSKRLAS ETPVSLFIPY SHHITDTIIS TKNAEYLSVW KIDGRSHQSA SEEDIFQWTK ELNNTLRGIA SANLSLWTHI VRRRVYEYPD SAFDQMFCYQ LDEKYRQSFT GYNLMVNDLY LTVIFQPIAD KVMSFFSQHE RETLDQKKMR QESCIKALND INNTLAQSLK RYGAELLGAY EKNGHAYSSA LEFLGMLING EYRPMPICYE RFADYMSINR PFFSKWGALG ELRTSTGMRR FGMLEIKEYD TTTKPGQLNA LLESDFEFVL TQSFSVLSRH AAKDYLQRHQ RNLIDARDVA TSQIEQIDEA LNQLVSGHFV MGEHHCTLTI YGETVQQVRD YMAKANAILL DAAVLPKPVD LAIEAGYWAQ LPANWKWRPR PAPITSLNFL SFSSFHNFMS GKPTGNPWGP AVTILKTNSK TPLYFNFHAS KIEEDSTDKR LLGNTALIGQ SGSGKTVLLG FLLAQAQKFK PTTVVFDKDR GMEIIIRAMG GRYLTFKPGK QSGFNPFQLP PTQDNLIFLK QFVKKLAEAG GEVTHHDEEE INQAVMSVMS SNIDRSLRRL SFLVQFLPNP HLNDYNAHPS VHARLIKWCE GGDYGWLFDN PTDALDLSTH QIYGFDITEF LDNSETRTPV MMYLLYRTEG MISGQRFMYI FDEFWKPLQD PYFEDLAKNK QKTIRKQNGI FVYATQEPSD ALESNIAKTL IQQCATYIFL ANPKANYEDY TQGFKLTDTE FELVKGLGEF SRQFLIKQGD QSALAELNLG KFHMTIDGKT KECDFSEELL VLSGTPDNAE LAESIITKVG DDPAIWLPVF LQHIKTDRRE T

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgg ctgttgaaaa cagcaaacgt cttgcctcag agacacctgt aagtctgttc ataccctatt cgcaccatat tacagataca attatctcta caaaaaatgc ggaatatcta tcggtttgga aaattgatgg acgttcgcat caaagtgcgt cagaggaaga tatttttcag tggaccaaag agctaaataa taccctacgt ggtattgcat cagccaattt atcattgtgg acacatatcg tacgccgccg tgtctatgaa tatcctgatt cagcatttga ccaaatgttt tgctatcagc ttgatgaaaa gtatcgacaa agtttcactg gttataatct tatggtgaat gacctgtatt tgactgttat tttccaaccg atagcagata aagttatgtc attcttttct cagcatgaac gtgaaacgct cgatcaaaaa aagatgcggc aggaatcatg tatcaaagcc ctcaacgaca ttaacaacac tttagctcaa tcgttaaaac ggtacggtgc agagcttctt ggtgcttatg aaaaaaatgg ccatgcctat tcttctgcgc ttgaattcct tggaatgctt attaacggcg aatataggcc tatgccaatc tgttatgagc gttttgccga ctatatgtct attaatcggc catttttctc aaaatggggt gctcttggag aattacgcac aagcactgga atgcgtcgat ttggaatgct ggaaattaag gaatacgaca caacaaccaa accaggtcaa ttaaatgctt tgctggaaag tgatttcgaa tttgtcttga cacaaagctt ttcagtactt tcgcgacatg ctgctaagga ttatttgcaa cgccaccaac gtaatctgat cgatgctcgc gatgtggcaa caagccaaat tgagcaaatt gatgaagcat taaaccagct tgtcagtgga cactttgtta tgggtgaaca tcactgcaca ttgaccattt atggtgagac agttcaacaa gtccgtgact atatggcaaa agcaaatgca atattgctag atgctgcagt attgccaaaa cctgtagatt tggctataga agctggttat tgggcacaac tgcccgcaaa ttggaaatgg cggccgcgtc cagcaccaat cacgtcacta aactttttat cattttcatc ttttcataat tttatgtcag gcaaaccaac tggaaatcca tgggggccgg cagtcacaat tctgaaaaca aacagcaaaa caccgcttta ttttaatttt catgcttcta aaattgagga agattcaaca gataaacgct tacttggcaa taccgccctt atcgggcaat ctggttccgg taaaacagtg ctacttggtt ttctgttagc gcaagcacaa aagtttaaac caacaaccgt tgtttttgat aaagatcgcg gcatggaaat tattattaga gctatgggtg gtcgatatct aacattcaag cctggtaagc aaagtggttt caatcctttt caacttcctc caacacaaga taacctgatt tttctaaaac agtttgttaa aaaattagct gaagctggtg gtgaagttac acatcacgat gaagaagaaa ttaaccaagc tgtcatgtct gtcatgagca gcaacattga cagatcgctg cgtcgtttgt cttttcttgt acaattctta ccaaatccac atttaaatga ctataatgct cacccatctg ttcatgctcg tttgataaaa tggtgcgaag gtggtgatta tgggtggtta tttgataacc ctactgatgc actcgatctt tcaacacacc aaatttacgg ttttgatatt actgagttct tagataactc agaaacgcgt acaccagtca tgatgtatct gctttaccgc acagaaggaa tgattagcgg acagcgattt atgtatattt ttgatgagtt ttggaaacct ttgcaagatc catattttga agatctagca aaaaataagc aaaaaactat ccgtaaacaa aatggtattt ttgtttacgc tacacaagaa cccagtgatg ccttggaaag caatatagcc aagacactta ttcaacaatg tgcaacctac attttcttgg ccaatcccaa agcaaattac gaagattata cacaaggttt taaactaaca gatactgaat ttgaactggt taaaggcctt ggtgaattta gtcgccagtt tctcatcaag caaggcgacc aatctgcact tgcagagtta aatctcggca aatttcatat gacaatcgat ggaaaaacta aagaatgcga ctttagtgaa gagcttttgg tattatcagg tacacctgat aatgcagaat tggctgaaag catcattact aaagtgggtg atgatccagc aatatggtta ccagtttttt tacaacatat aaaaactgac aggagggaaa catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat