BabiA.18407.a

TrwE component of type IV secretion system (Trw type IV secretion machinery)

CENTER ID: BabiA.18407.a
ORGANISM: Bartonella birtlesii
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BabiA.18407.a.B2.GE35914 soluble domain 65 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: D0AAZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFDNKKGDEI NKNAERGYAE QKHIEGAYGS SELGLERRPI IPGARALLMV GLIVIIAVPI ALTWKALKMR NSIEVEEEKP QQTVQQIIPN YVPRVIEEPK VEEEEKKPED STQEIPATLA KLLQTTIPPH LIKDSEELAR KRMLSSGLNS GGSSEGSTEP SKNNASNDDR NSGALFDHLQ PVRLGHSQAV QLNNRDLLIT QGTQIDCTLE TKIITTQPGM TTCHLTRDVY STSGRVVLLD RGSKVVGFYP SGIQQGQTRV FVQWSRIETP SGVIVNLDSP GTGPLGEAGI GGWIDTHFWQ RFGGAIMVSI IGDLGEWVKD KIWRKDRDNK ENKERVQSQG VHNAESVVTD VLQNSINITP TLYKNQGERV NIFVARDLDF SDVYSLVTR
NT Sequence
atgtttgaca ataagaaagg agatgagatc aataaaaacg cggaacgagg ttacgccgag caaaaacata ttgaaggtgc ctatggtagt tctgagttag gtttggagcg tcgtccaata attcccggtg ctcgtgcatt attaatggta ggactcattg tcataatagc ggtaccaatt gctctgactt ggaaagcttt aaaaatgcgc aatagtatag aagttgagga agaaaaacct caacagacag tgcagcaaat cataccaaac tatgtccctc gcgttataga ggagccaaaa gtagaagaag aagaaaaaaa acctgaagat tcaacacagg aaattccagc aaccttagca aaacttcttc aaacaacaat tcctccacat ttgatcaaag attctgaaga gttggcgcgc aaacgtatgc ttagttccgg tcttaacagt ggcggtagta gtgaaggctc gacagaaccg tccaaaaata acgcatctaa tgacgataga aatagtggtg cattatttga tcaccttcag ccggtacgct taggtcattc acaagcagta cagctcaata accgtgatct cttgatcaca caaggaacac agatagattg tacactagaa acaaaaatta tcacaaccca accgggaatg acaacgtgtc atttaacacg tgatgtttat tctacaagtg gtcgggttgt tttgctcgat cgtggctcta aggttgttgg tttttatcca agtggcatac aacaaggaca aacacgcgtt ttcgtgcaat ggtcgcgcat tgaaacacct tccggtgtta tcgttaatct tgactcaccc ggtacaggtc ctcttggaga agctggtatt ggtggttgga ttgatacgca tttttggcaa cgatttggcg gagcaattat ggtgagcatc attggtgatt tgggagaatg ggtaaaagac aaaatttggc gaaaagatcg agataataaa gagaataagg aaagagtaca atcccaaggc gtacataacg ctgaatcggt cgtgactgat gttctccaaa actcaatcaa cattacacca acactttata aaaaccaagg cgagcgcgtg aatatttttg ttgctcgaga tctggatttc agtgatgtct acagcctcgt tacacgttaa
Details for BabiA.18407.a.B2.GE35914
HARVESTED ON: 8/28/2012
SEQUENCED ON: 10/23/2013
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMK ALKMRNSIEV EEEKPQQTVQ QIIPNYVPRV IEEPKVEEEE KKPEDSTQEI PATLAKLLQT TIPPHLIKDS EELARKRMLS SGLNSGGSSE GSTEPSKNNA SNDDRNSGAL FDHLQPVRLG QSQAVQLNNR DLLITQGTQI DCTLETKIIT TQPGMTTCHL TRDVYSTSGR VVLLDRGSKV VGFYQSGIQQ GQTRVFVQWS RIETPSGVIV NLDSPGTGPL GEAGIGGWID THFWQRFGGA IMVSIIGDLG EWVKDKIWRK DRDNKENXGK STIPRRT
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa gctttaaaaa tgcgcaatag tatagaagtt gaggaagaaa aacctcaaca gacagtgcag caaatcatac caaactatgt ccctcgcgtt atagaggagc caaaagtaga agaagaagaa aaaaaacctg aagattcaac acaggaaatt ccagcaacct tagcaaaact tcttcaaaca acaattcctc cacatttgat caaagattct gaagagttgg cgcgcaaacg tatgcttagt tccggtctta acagtggcgg tagtagtgaa ggctcgacag aaccgtcaaa aaataacgca tctaatgacg atagaaatag tggtgcatta tttgatcacc ttcagccggt acgcttaggt caatcacaag cagtacagct caataaccgt gatctcttga tcacacaagg aacacagata gattgtacac tagaaacaaa aattatcaca acccaaccgg gaatgacaac gtgtcattta acacgtgatg tttattctac aagtggtcgg gttgttttgc tcgatcgtgg ctctaaggtt gttggttttt atcaaagtgg catacaacaa ggacaaacac gcgttttcgt gcaatggtcg cgcattgaaa caccttccgg tgttatcgtt aatcttgact cacccggtac aggtcctctt ggagaagctg gtattggtgg ttggattgat acgcattttt ggcaacgatt tggcggagca attatggtga gcatcattgg tgatttggga gaatgggtaa aagacaaaat ttggcgaaaa gatcgagata ataaagagaa taanggaaag agtacaatcc caaggcgtac ataacgctga atcggtcgtg actgatgttc tccaaaactc aatcnacatt acnccnacac tttataaaaa cnnnggcgag cgcgtgnnna tttttgtngc tcgagatctg gntttcagtg ang
Expected Protein Sequence
MAHHHHHHMK ALKMRNSIEV EEEKPQQTVQ QIIPNYVPRV IEEPKVEEEE KKPEDSTQEI PATLAKLLQT TIPPHLIKDS EELARKRMLS SGLNSGGSSE GSTEPSKNNA SNDDRNSGAL FDHLQPVRLG HSQAVQLNNR DLLITQGTQI DCTLETKIIT TQPGMTTCHL TRDVYSTSGR VVLLDRGSKV VGFYPSGIQQ GQTRVFVQWS RIETPSGVIV NLDSPGTGPL GEAGIGGWID THFWQRFGGA IMVSIIGDLG EWVKDKIWRK DRDNKENKER VQSQGVHNAE SVVTDVLQNS INITPTLYKN QGERVNIFVA RDLDFSDVYS LVTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag ctttaaaaat gcgcaatagt atagaagttg aggaagaaaa acctcaacag acagtgcagc aaatcatacc aaactatgtc cctcgcgtta tagaggagcc aaaagtagaa gaagaagaaa aaaaacctga agattcaaca caggaaattc cagcaacctt agcaaaactt cttcaaacaa caattcctcc acatttgatc aaagattctg aagagttggc gcgcaaacgt atgcttagtt ccggtcttaa cagtggcggt agtagtgaag gctcgacaga accgtccaaa aataacgcat ctaatgacga tagaaatagt ggtgcattat ttgatcacct tcagccggta cgcttaggtc attcacaagc agtacagctc aataaccgtg atctcttgat cacacaagga acacagatag attgtacact agaaacaaaa attatcacaa cccaaccggg aatgacaacg tgtcatttaa cacgtgatgt ttattctaca agtggtcggg ttgttttgct cgatcgtggc tctaaggttg ttggttttta tccaagtggc atacaacaag gacaaacacg cgttttcgtg caatggtcgc gcattgaaac accttccggt gttatcgtta atcttgactc acccggtaca ggtcctcttg gagaagctgg tattggtggt tggattgata cgcatttttg gcaacgattt ggcggagcaa ttatggtgag catcattggt gatttgggag aatgggtaaa agacaaaatt tggcgaaaag atcgagataa taaagagaat aaggaaagag tacaatccca aggcgtacat aacgctgaat cggtcgtgac tgatgttctc caaaactcaa tcaacattac accaacactt tataaaaacc aaggcgagcg cgtgaatatt tttgttgctc gagatctgga tttcagtgat gtctacagcc tcgttacacg ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat