BabiA.18004.a

Succinate dehydrogenase FAD subunit (EC 1.3.99.1) (Trw type IV secretion machinery)

CENTER ID: BabiA.18004.a
ORGANISM: Bartonella birtlesii
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BabiA.18004.a.B1.GE35910 full length 1 614
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: D0AB00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGTVSSLGA RAGNAPYCTV DHKFDVVVVG AGGAGLRATL GMAEQGLKTA CITKVFPTRS HTVAAQGGIA ASLSNMGPDN WQWHLYDTVK GSDWLGDTDA MEYLVRNAPA AVYELEHYGV PFSRTQEGKI YQRPFGGHTT QFGEGPPVQR TCAAADRTGH AILHTLYGQS LKHNAQFFIE YFALDLIMTD GVCTGVVAWN LDDGTIHRFS AKMVVLATGG YGRAYFSATS AHTCTGDGGG MIARAGLPLQ DMEFVQFHPT GIYGSGCLIT EGARGEGGYL INSEGERFME RYAPSFKDLA SRDLVSRCIT LEIREGRGVG PRLDHIHLVL NHIDPAILHE RLPGISESAR IFAGVDVTRE PIPILPTVHY NMGGIPTNYY GEVLNPTADS PDRIQPGLMA VGEAGCASVH GANRLGSNSL IDLVVFGRAA AIRAGEVIDR DAEIPPLNEA AVDEIMARFD RLRFAQGATP TAVLREKMQR TMQEDAAVFR TEDSLQQGCR RISQIWDGLA DLKVTDRSLI WNSDLVETLE LENLMANAIT TVYSAEARLE SRGAHAREDY PERDDKNWRK HTLSHLSKEG KVTLSYRPVH VDPLTAEADG GIDLKRIAPK KRVY
NT Sequence
atggcaggta cagtatcatc tctaggcgcg agagcaggga atgctcctta ttgtactgtc gatcataaat tcgatgttgt ggttgttggg gcaggtggtg caggattgcg ggcaacactt gggatggcag agcaaggatt aaaaacagct tgtattacaa aagtttttcc aacaagatca catactgttg cggcgcaagg gggtattgca gcttcacttt ccaatatggg acctgacaat tggcaatggc atttgtatga tactgtaaag ggatctgatt ggttgggtga taccgatgca atggaatatt tggtgcgtaa tgcacctgct gctgtctatg aattagaaca ttatggggtg cctttttcac gtacgcaaga aggtaaaatt tatcaacgtc cttttggtgg gcatacaaca caatttggtg aaggtccacc tgttcagcgg acttgtgcag cagctgatcg cacaggacat gccattttgc atacacttta tggacagagt ttaaagcata atgcgcagtt ttttattgaa tattttgcac ttgatctcat catgaccgat ggagtctgca ccggtgtggt ggcatggaat cttgatgatg gcacgataca ccgtttttct gccaaaatgg ttgttctcgc aaccggtggt tatgggcgtg cttatttttc agcaacatcg gctcatactt gtacgggtga tgggggtgga atgattgcac gtgccggatt accactgcag gatatggaat ttgttcagtt tcatcccaca gggatttatg gttctggctg tctcattaca gaaggggcac ggggtgaagg tggttatttg attaattctg agggtgaacg ttttatggag cgctatgcac cttcctttaa agatttggct tcacgggatt tggtatcgcg ttgtataacc cttgaaattc gcgaagggcg cggtgtcggt ccaagattgg atcatattca tttggttctc aatcacattg atcccgccat attgcacgaa cgtttaccag gaatttccga atcagcacgc atttttgctg gcgttgatgt gacaagagaa cctattccta tccttcctac ggtgcattat aatatggggg gaattcctac caattattat ggagaggtac ttaatccaac ggctgattcg cctgatcgta ttcaaccagg tttgatggca gtgggagagg ctggatgtgc ttctgttcat ggtgctaatc gtttgggatc aaattcgtta attgatcttg ttgtatttgg tcgtgctgct gcaattcgtg ccggtgaagt cattgatcga gatgcggaaa ttcctcctct caatgaggcg gcggttgatg aaattatggc acgttttgat cgtttacgtt ttgctcaagg agcaacacca acagctgtat tgcgtgaaaa aatgcaacgc actatgcaag aagatgctgc tgttttccgt acggaagatt ctttgcaaca aggatgtcgg cggataagtc aaatttggga tggattagct gatcttaaag tcacagatcg ctcactgata tggaattctg atttggttga aacattagag cttgaaaatc tgatggctaa tgcaattacc actgtttatt ccgcagaagc gcgtttggaa agtcgaggcg ctcatgcacg tgaagattat ccagagcgtg acgataaaaa ttggcgcaaa catacgcttt cacatttatc aaaagaaggt aaggtaactt tgtcctatcg acctgttcat gttgatccat taacagctga agcagatggt ggtattgatt taaaacgcat tgcacctaaa aagcgtgttt attaa
Details for BabiA.18004.a.B1.GE35910
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 45
Validated AA Sequence
MAHHHHHHMA GTVSSLGARA GNAPYCTVDH KFDVVVVGAG GAGLRATLGM AEQGLKTACI TKVFPTRSHT VAAQGGIAAS LSNMGPDNWQ WHLYDTVKGS DWLGDTDAME YLVRNAPAAV YELEHYGVPF SRTQEGKIYQ RPFGGHTTQF GEGPPVQRTC AAADRTGHAI LHTLYGQSLK HNAQFFIEYF ALDLIMTDGV CTGVVAWNLD DGTIHRFSAK MVVLATGGYG RAYFSATSAH TCTGDGGGMI ARAGXPLXDM EFVQFHPTGX YGSGCLITEX GTG
Validated NT Sequence
ttgtnnnnnt taagaaggag atataccatg gctcaccacc accaccacca tatggcaggt acagtatcat ctctaggcgc gagagcaggg aatgctcctt attgtactgt cgatcataaa ttcgatgttg tggttgttgg ggcaggtggt gcaggattgc gggcaacact tgggatggca gagcaaggat taaaaacagc ttgtattaca aaagtttttc caacaagatc acatactgtt gcggcgcaag ggggtattgc agcttcactt tccaatatgg gacctgacaa ttggcaatgg catttgtatg atactgtaaa gggatctgat tggttgggtg ataccgatgc aatggaatat ttggtgcgta atgcacctgc tgctgtctat gaattagaac attatggggt gcctttttca cgtacgcaag aaggtaaaat ttatcaacgt ccttttggtg ggcatacaac acaatttggt gaaggtccac ctgttcagcg gacttgtgca gcagctgatc gcacaggaca tgccattttg catacacttt atggacagag tttaaagcat aatgcgcagt tttttattga atattttgca cttgatctca tcatgaccga tggagtctgc accggtgtgg tggcatggaa tcttgatgat ggcacgatac accgtttttc tgccaaaatg gttgttctcg caaccggtgg ttatgggcgt gcttattttt cagcaacatc ggctcatact tgtacgggtg atgggggtgg aatgattgca cgtgccggan taccactgca ngatatggaa tttgttcagt ttcatcccac agggntttat ggttctggct gtctcattac agaanggggc acggggtgaa ggtggttatt tgattaattc tgagggtgaa cgttttatgg agcgctatgc accttccttt nanatttggc ttcncgggat ttggtatcgc gttgtannan ccntngaaan tcgcgaaggg cgcnnnnnnn nnnannntca nattcnttng nnnancncnn nntncnnnnn ntnnannann tnncnnnntn nnancancnn tnnncnnnnn nnannnnnnn nnnnnnnnnn nnnnnnnann nnnnnngnnn nnnnnnnnnn nnnnnnnnnn nntnnnnnnn ccc
Expected Protein Sequence
MAHHHHHHMA GTVSSLGARA GNAPYCTVDH KFDVVVVGAG GAGLRATLGM AEQGLKTACI TKVFPTRSHT VAAQGGIAAS LSNMGPDNWQ WHLYDTVKGS DWLGDTDAME YLVRNAPAAV YELEHYGVPF SRTQEGKIYQ RPFGGHTTQF GEGPPVQRTC AAADRTGHAI LHTLYGQSLK HNAQFFIEYF ALDLIMTDGV CTGVVAWNLD DGTIHRFSAK MVVLATGGYG RAYFSATSAH TCTGDGGGMI ARAGLPLQDM EFVQFHPTGI YGSGCLITEG ARGEGGYLIN SEGERFMERY APSFKDLASR DLVSRCITLE IREGRGVGPR LDHIHLVLNH IDPAILHERL PGISESARIF AGVDVTREPI PILPTVHYNM GGIPTNYYGE VLNPTADSPD RIQPGLMAVG EAGCASVHGA NRLGSNSLID LVVFGRAAAI RAGEVIDRDA EIPPLNEAAV DEIMARFDRL RFAQGATPTA VLREKMQRTM QEDAAVFRTE DSLQQGCRRI SQIWDGLADL KVTDRSLIWN SDLVETLELE NLMANAITTV YSAEARLESR GAHAREDYPE RDDKNWRKHT LSHLSKEGKV TLSYRPVHVD PLTAEADGGI DLKRIAPKKR VY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag gtacagtatc atctctaggc gcgagagcag ggaatgctcc ttattgtact gtcgatcata aattcgatgt tgtggttgtt ggggcaggtg gtgcaggatt gcgggcaaca cttgggatgg cagagcaagg attaaaaaca gcttgtatta caaaagtttt tccaacaaga tcacatactg ttgcggcgca agggggtatt gcagcttcac tttccaatat gggacctgac aattggcaat ggcatttgta tgatactgta aagggatctg attggttggg tgataccgat gcaatggaat atttggtgcg taatgcacct gctgctgtct atgaattaga acattatggg gtgccttttt cacgtacgca agaaggtaaa atttatcaac gtccttttgg tgggcataca acacaatttg gtgaaggtcc acctgttcag cggacttgtg cagcagctga tcgcacagga catgccattt tgcatacact ttatggacag agtttaaagc ataatgcgca gttttttatt gaatattttg cacttgatct catcatgacc gatggagtct gcaccggtgt ggtggcatgg aatcttgatg atggcacgat acaccgtttt tctgccaaaa tggttgttct cgcaaccggt ggttatgggc gtgcttattt ttcagcaaca tcggctcata cttgtacggg tgatgggggt ggaatgattg cacgtgccgg attaccactg caggatatgg aatttgttca gtttcatccc acagggattt atggttctgg ctgtctcatt acagaagggg cacggggtga aggtggttat ttgattaatt ctgagggtga acgttttatg gagcgctatg caccttcctt taaagatttg gcttcacggg atttggtatc gcgttgtata acccttgaaa ttcgcgaagg gcgcggtgtc ggtccaagat tggatcatat tcatttggtt ctcaatcaca ttgatcccgc catattgcac gaacgtttac caggaatttc cgaatcagca cgcatttttg ctggcgttga tgtgacaaga gaacctattc ctatccttcc tacggtgcat tataatatgg ggggaattcc taccaattat tatggagagg tacttaatcc aacggctgat tcgcctgatc gtattcaacc aggtttgatg gcagtgggag aggctggatg tgcttctgtt catggtgcta atcgtttggg atcaaattcg ttaattgatc ttgttgtatt tggtcgtgct gctgcaattc gtgccggtga agtcattgat cgagatgcgg aaattcctcc tctcaatgag gcggcggttg atgaaattat ggcacgtttt gatcgtttac gttttgctca aggagcaaca ccaacagctg tattgcgtga aaaaatgcaa cgcactatgc aagaagatgc tgctgttttc cgtacggaag attctttgca acaaggatgt cggcggataa gtcaaatttg ggatggatta gctgatctta aagtcacaga tcgctcactg atatggaatt ctgatttggt tgaaacatta gagcttgaaa atctgatggc taatgcaatt accactgttt attccgcaga agcgcgtttg gaaagtcgag gcgctcatgc acgtgaagat tatccagagc gtgacgataa aaattggcgc aaacatacgc tttcacattt atcaaaagaa ggtaaggtaa ctttgtccta tcgacctgtt catgttgatc cattaacagc tgaagcagat ggtggtattg atttaaaacg cattgcacct aaaaagcgtg tttattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat