BabiA.17987.a
DNA polymerase III subunit beta (EC 2.7.7.7)
| CENTER ID: | BabiA.17987.a |
| ORGANISM: | Bartonella birtlesii |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BabiA.17987.a.B1.GE41235 | full length | 1 | 373 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BabiA.17987.a.B1.PS38344 | full length | 1 | 373 |
Structures
6DEG
DEPOSITED: 5/11/2018
DETERMINATION: XRay
CLONE: BabiA.17987.a.B1.GE41235
PROTEIN: BabiA.17987.a.B1.PS38344
6PTR
DEPOSITED: 7/16/2019
DETERMINATION: XRay
CLONE: BabiA.17987.a.B1.GE41235
PROTEIN: BabiA.17987.a.B1.PS38344
Publications by SSGCID
Exquisite selectivity of griselimycin extends to beta subunit of DNA polymerases from Gram-negative bacterial pathogens
Abendroth JA, Barrett L, Choi R, Dranow DM, Edwards TE, Hewitt SN, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Subramanian S, Udell HS, Van Voorhis W, Wallace E, Mayclin SJ, Hammerson B, Chun IKH, Barrett KF, Conrady DG, Mueller R, Tillery L, Lukat P, Blankenfeldt W, Higgins T, Bowatte K, Smutney H, Stigliano M, Fenwick MK, Craig J, Peng V, DeRocher AE
Commun Biol - 2024
volume 7, issue 1,
PMID: 39639052; PMCID: PMC11621423
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|1094552.3.peg.1290 |
| UniProt: | J1IY24 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRITVDRSQF FKSLGRVHRV VERRNTVPIL SNVLIDAENG SVQLKATDLD LEVTESFTVN IEKAGAITVP AYLLYDIVRK LPDGSEIVLS VDENQASAMS IVSGCTHFQL QCLPKIDFPE SLPGQFGCRF FLSASKLKHL LDCTQFAIST EETRYYLNGI YFHIVHDDVL KLRLVATDGH RLAQVDMEAP SGVDGMPGVI IPRKAVGELQ KLLSEEIDGD VCIELSETKI RFSLGSVVFT SKLVDGTFPD YQRVIPLGND RKLIVNRQDF SSAVDRVSTI SNDRGRAVKL TIEHGQLKLV VNNPDSGSAE DQLAATYTSE PLEIGFNSRY LLDIAGQLSS DEMVFMLSDA VAPALIRDNN NAEVLYVLMP VRV
NT Sequence
atgcgtatta ccgttgatcg cagccagttt tttaaatctc ttggtcgtgt tcaccgtgtg gtggaacgac gaaataccgt tcctatttta tctaatgtat taattgatgc agaaaatggg agtgtgcaat taaaagcaac ggatcttgat ttagaagtta cagaatcttt tacagtcaat attgaaaaag caggggcaat aactgttcca gcatatttat tgtatgatat agttcgaaaa cttcctgatg gtagtgagat tgtcttgtca gttgatgaga atcaagcaag tgctatgtct attgtttcag gttgtacaca ttttcagctt caatgtcttc cgaaaataga ttttccagag tctcttcctg gtcagtttgg ttgccgtttt tttctatcgg catcgaagtt aaagcatttg cttgattgta cgcaatttgc tatctctact gaagaaacac gttattatct taatggcatt tattttcata ttgtgcatga cgatgtttta aaattgcgtt tggttgcaac tgatggccac cgtttggcac aggttgatat ggaggccccc tcaggtgttg atggcatgcc aggtgttatt attcctcgta aggctgtagg ggagttgcaa aagcttcttt cagaagaaat tgatggtgat gtatgtatcg aactctcaga gacaaagatt cgtttttctt taggttcagt ggtttttaca tcaaagttag ttgatggaac atttccagat tatcaacgcg ttattcccct tggaaatgat agaaaattga ttgtcaatag acaggatttt tcttctgctg ttgaccgtgt ttcaacaatt tcgaatgacc gtgggcgtgc ggtaaaattg acaattgagc atgggcaatt aaagcttgtc gtgaataatc ctgattcggg tagtgcggag gatcaattag cggcaactta tacatcagaa ccacttgaaa tagggtttaa ttcacgttat cttttggata tcgctgggca actttcaagt gatgagatgg tttttatgtt gtcagatgct gtagcaccag ctctcattcg tgataataat aacgcagagg tgctttatgt actgatgccc gttcgcgttt ag
Details for BabiA.17987.a.B1.GE41235
| HARVESTED ON: | 3/8/2017 |
| SEQUENCED ON: | 3/9/2017 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMR ITVDRSQFFK SLGRVHRVVE RRNTVPILSN VLIDAENGSV QLKATDLDLE VTESFTVNIE KAGAITVPAY LLYDIVRKLP DGSEIVLSVD ENQASAMSIV SGCTHFQLQC LPKIDFPESL PGQFGCRFFL SASKLKHLLD CTQFAISTEE TRYYLNGIYF HIVHDDVLKL RLVATDGHRL AQVDMEAPSG VDGMPGVIIP RKAVGELQKL LSEEIDGDVC IELSETKIRF SLGSVVFTSK LVDGTFPDYQ RVIPLGNDRK LIVNRQDFSS AVDRVSTISN DRGRAVKLTI EHGQLKLVVN NPDSGSAEDQ LAATYTSEPL EIGFNSRYLL DIAGQLSSDE MVFMLSDAVA PALIRDNNNA EV
Validated NT Sequence
atggctcacc accaccacca ccatatgcgt attaccgttg atcgcagtca gttttttaaa tctcttggtc gtgttcaccg tgtggtggaa cgacgaaata ccgttcctat tttatctaat gtattaattg atgcagaaaa tgggagtgtg caattaaaag caacggatct tgatttagaa gttacagaat cttttacagt caatattgaa aaagcagggg caataactgt tccagcatat ttattgtatg atatagttcg aaaacttcct gatggtagtg agattgtctt gtcagttgat gagaatcaag caagtgctat gtctattgtt tcaggttgta cacattttca gcttcaatgt cttccgaaaa tagattttcc agagtctctt cctggtcagt ttggttgccg tttttttcta tcggcatcga agttaaagca tttgcttgat tgtacgcaat ttgctatctc tactgaagaa acacgttatt atcttaatgg catttatttt catattgtgc atgacgatgt tttaaaattg cgtttggttg caactgatgg ccaccgtttg gcacaggttg atatggaggc cccctcaggt gttgatggca tgccaggtgt tattattcct cgtaaggctg taggggagtt gcaaaagctt ctttcagaag aaattgatgg tgatgtatgt atcgaactct cagagacaaa gattcgtttt tctttaggtt cagtggtttt tacatcaaag ttagttgatg gaacatttcc agattatcaa cgcgttattc cccttggaaa tgatagaaaa ttgattgtca atagacagga tttttcttct gctgttgacc gtgtttcaac aatttcgaat gaccgtgggc gtgcggtaaa attgacaatt gagcatgggc aattaaagct tgtcgtgaat aatcctgatt cgggtagtgc ggaggatcaa ttagcggcaa cttatacatc agaaccactt gaaatagggt ttaattcacg ttatcttttg gatatcgctg ggcaactttc aagtgatgag atggttttta tgttgtcaga tgctgtagca ccagctctca ttcgtgataa taataacgca gaggtgct
Expected Protein Sequence
MAHHHHHHMR ITVDRSQFFK SLGRVHRVVE RRNTVPILSN VLIDAENGSV QLKATDLDLE VTESFTVNIE KAGAITVPAY LLYDIVRKLP DGSEIVLSVD ENQASAMSIV SGCTHFQLQC LPKIDFPESL PGQFGCRFFL SASKLKHLLD CTQFAISTEE TRYYLNGIYF HIVHDDVLKL RLVATDGHRL AQVDMEAPSG VDGMPGVIIP RKAVGELQKL LSEEIDGDVC IELSETKIRF SLGSVVFTSK LVDGTFPDYQ RVIPLGNDRK LIVNRQDFSS AVDRVSTISN DRGRAVKLTI EHGQLKLVVN NPDSGSAEDQ LAATYTSEPL EIGFNSRYLL DIAGQLSSDE MVFMLSDAVA PALIRDNNNA EVLYVLMPVR V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgta ttaccgttga tcgcagccag ttttttaaat ctcttggtcg tgttcaccgt gtggtggaac gacgaaatac cgttcctatt ttatctaatg tattaattga tgcagaaaat gggagtgtgc aattaaaagc aacggatctt gatttagaag ttacagaatc ttttacagtc aatattgaaa aagcaggggc aataactgtt ccagcatatt tattgtatga tatagttcga aaacttcctg atggtagtga gattgtcttg tcagttgatg agaatcaagc aagtgctatg tctattgttt caggttgtac acattttcag cttcaatgtc ttccgaaaat agattttcca gagtctcttc ctggtcagtt tggttgccgt ttttttctat cggcatcgaa gttaaagcat ttgcttgatt gtacgcaatt tgctatctct actgaagaaa cacgttatta tcttaatggc atttattttc atattgtgca tgacgatgtt ttaaaattgc gtttggttgc aactgatggc caccgtttgg cacaggttga tatggaggcc ccctcaggtg ttgatggcat gccaggtgtt attattcctc gtaaggctgt aggggagttg caaaagcttc tttcagaaga aattgatggt gatgtatgta tcgaactctc agagacaaag attcgttttt ctttaggttc agtggttttt acatcaaagt tagttgatgg aacatttcca gattatcaac gcgttattcc ccttggaaat gatagaaaat tgattgtcaa tagacaggat ttttcttctg ctgttgaccg tgtttcaaca atttcgaatg accgtgggcg tgcggtaaaa ttgacaattg agcatgggca attaaagctt gtcgtgaata atcctgattc gggtagtgcg gaggatcaat tagcggcaac ttatacatca gaaccacttg aaatagggtt taattcacgt tatcttttgg atatcgctgg gcaactttca agtgatgaga tggtttttat gttgtcagat gctgtagcac cagctctcat tcgtgataat aataacgcag aggtgcttta tgtactgatg cccgttcgcg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BabiA.17987.a.B1.PS38344
| PURIFICATION DATe: | 8/30/2017 |
| CONCENTRATION: | 13.7mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR ITVDRSQFFK SLGRVHRVVE RRNTVPILSN VLIDAENGSV QLKATDLDLE VTESFTVNIE KAGAITVPAY LLYDIVRKLP DGSEIVLSVD ENQASAMSIV SGCTHFQLQC LPKIDFPESL PGQFGCRFFL SASKLKHLLD CTQFAISTEE TRYYLNGIYF HIVHDDVLKL RLVATDGHRL AQVDMEAPSG VDGMPGVIIP RKAVGELQKL LSEEIDGDVC IELSETKIRF SLGSVVFTSK LVDGTFPDYQ RVIPLGNDRK LIVNRQDFSS AVDRVSTISN DRGRAVKLTI EHGQLKLVVN NPDSGSAEDQ LAATYTSEPL EIGFNSRYLL DIAGQLSSDE MVFMLSDAVA PALIRDNNNA EV
Validated NT Sequence
atggctcacc accaccacca ccatatgcgt attaccgttg atcgcagtca gttttttaaa tctcttggtc gtgttcaccg tgtggtggaa cgacgaaata ccgttcctat tttatctaat gtattaattg atgcagaaaa tgggagtgtg caattaaaag caacggatct tgatttagaa gttacagaat cttttacagt caatattgaa aaagcagggg caataactgt tccagcatat ttattgtatg atatagttcg aaaacttcct gatggtagtg agattgtctt gtcagttgat gagaatcaag caagtgctat gtctattgtt tcaggttgta cacattttca gcttcaatgt cttccgaaaa tagattttcc agagtctctt cctggtcagt ttggttgccg tttttttcta tcggcatcga agttaaagca tttgcttgat tgtacgcaat ttgctatctc tactgaagaa acacgttatt atcttaatgg catttatttt catattgtgc atgacgatgt tttaaaattg cgtttggttg caactgatgg ccaccgtttg gcacaggttg atatggaggc cccctcaggt gttgatggca tgccaggtgt tattattcct cgtaaggctg taggggagtt gcaaaagctt ctttcagaag aaattgatgg tgatgtatgt atcgaactct cagagacaaa gattcgtttt tctttaggtt cagtggtttt tacatcaaag ttagttgatg gaacatttcc agattatcaa cgcgttattc cccttggaaa tgatagaaaa ttgattgtca atagacagga tttttcttct gctgttgacc gtgtttcaac aatttcgaat gaccgtgggc gtgcggtaaa attgacaatt gagcatgggc aattaaagct tgtcgtgaat aatcctgatt cgggtagtgc ggaggatcaa ttagcggcaa cttatacatc agaaccactt gaaatagggt ttaattcacg ttatcttttg gatatcgctg ggcaactttc aagtgatgag atggttttta tgttgtcaga tgctgtagca ccagctctca ttcgtgataa taataacgca gaggtgct
Expressed Protein Sequence
MAHHHHHHMR ITVDRSQFFK SLGRVHRVVE RRNTVPILSN VLIDAENGSV QLKATDLDLE VTESFTVNIE KAGAITVPAY LLYDIVRKLP DGSEIVLSVD ENQASAMSIV SGCTHFQLQC LPKIDFPESL PGQFGCRFFL SASKLKHLLD CTQFAISTEE TRYYLNGIYF HIVHDDVLKL RLVATDGHRL AQVDMEAPSG VDGMPGVIIP RKAVGELQKL LSEEIDGDVC IELSETKIRF SLGSVVFTSK LVDGTFPDYQ RVIPLGNDRK LIVNRQDFSS AVDRVSTISN DRGRAVKLTI EHGQLKLVVN NPDSGSAEDQ LAATYTSEPL EIGFNSRYLL DIAGQLSSDE MVFMLSDAVA PALIRDNNNA EVLYVLMPVR V
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTA TTACCGTTGA TCGCAGCCAG TTTTTTAAAT CTCTTGGTCG TGTTCACCGT GTGGTGGAAC GACGAAATAC CGTTCCTATT TTATCTAATG TATTAATTGA TGCAGAAAAT GGGAGTGTGC AATTAAAAGC AACGGATCTT GATTTAGAAG TTACAGAATC TTTTACAGTC AATATTGAAA AAGCAGGGGC AATAACTGTT CCAGCATATT TATTGTATGA TATAGTTCGA AAACTTCCTG ATGGTAGTGA GATTGTCTTG TCAGTTGATG AGAATCAAGC AAGTGCTATG TCTATTGTTT CAGGTTGTAC ACATTTTCAG CTTCAATGTC TTCCGAAAAT AGATTTTCCA GAGTCTCTTC CTGGTCAGTT TGGTTGCCGT TTTTTTCTAT CGGCATCGAA GTTAAAGCAT TTGCTTGATT GTACGCAATT TGCTATCTCT ACTGAAGAAA CACGTTATTA TCTTAATGGC ATTTATTTTC ATATTGTGCA TGACGATGTT TTAAAATTGC GTTTGGTTGC AACTGATGGC CACCGTTTGG CACAGGTTGA TATGGAGGCC CCCTCAGGTG TTGATGGCAT GCCAGGTGTT ATTATTCCTC GTAAGGCTGT AGGGGAGTTG CAAAAGCTTC TTTCAGAAGA AATTGATGGT GATGTATGTA TCGAACTCTC AGAGACAAAG ATTCGTTTTT CTTTAGGTTC AGTGGTTTTT ACATCAAAGT TAGTTGATGG AACATTTCCA GATTATCAAC GCGTTATTCC CCTTGGAAAT GATAGAAAAT TGATTGTCAA TAGACAGGAT TTTTCTTCTG CTGTTGACCG TGTTTCAACA ATTTCGAATG ACCGTGGGCG TGCGGTAAAA TTGACAATTG AGCATGGGCA ATTAAAGCTT GTCGTGAATA ATCCTGATTC GGGTAGTGCG GAGGATCAAT TAGCGGCAAC TTATACATCA GAACCACTTG AAATAGGGTT TAATTCACGT TATCTTTTGG ATATCGCTGG GCAACTTTCA AGTGATGAGA TGGTTTTTAT GTTGTCAGAT GCTGTAGCAC CAGCTCTCAT TCGTGATAAT AATAACGCAG AGGTGCTTTA TGTACTGATG CCCGTTCGCG TTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT