SSGCID: Target Page

BabiA.17384.a

TrwF component of type IV secretion system (Trw type IV secretion machinery)

CENTER ID:	BabiA.17384.a
ORGANISM:	Bartonella birtlesii
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BabiA.17384.a.B2.GE35908	mature protein		24	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1094552.3.peg.1090
UniProt:	D0AAZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKLTFLALL SSFSFLYTVP VQALKTPSNS QYDHRIRYVT YNVADVVQIE TVLGVATHII LEEGERYITH AFGDSDAYAF AHKGRHIFIK PKAELANTNL IVVTDKRSYK FRLQFRNDRA GATYELAFHY PDTNTQKSEA NSQHLDIERG FHQGVKGYNL SYTMSGNQDI APINAWDNGR ITYFKFPANM DMPSIYLVDA EGNESLIPRT VIGSSNDIIA VHKVNPKWLI RLGKRALAVF NEAYDPNGVA NTTGTVSSMV HRVNKGGK

NT Sequence

atgaaaaaac ttacgttcct tgccttacta tcttcatttt cctttcttta cacggtgcct gtacaagcac tcaaaacccc atcaaattca caatatgatc atcgcattcg ctatgtaaca tataatgtgg ctgatgtggt tcaaattgaa accgttcttg gggtggcaac acatatcatc cttgaagagg gtgaacgcta catcacgcat gcttttggtg attctgatgc ttatgccttt gcacataaag gacgacatat ctttattaaa cccaaagcag aattagctaa tacgaatctc atcgttgtaa cagacaaacg tagctataaa tttcgattac agttccgaaa tgatcgtgct ggagccacct acgaattagc tttccattat cctgatacaa atactcaaaa gtcagaagca aacagccaac accttgatat tgaacgcggt tttcaccaag gtgtaaaagg ctataacctc agctatacca tgagtggcaa ccaagatatt gctccaatta atgcttggga caatgggcgt attacctatt tcaaatttcc tgccaacatg gatatgccat caatttacct agtagatgct gaaggtaacg agagcttaat accacgtacc gttataggca gttccaatga tattattgcc gttcataagg tcaatcccaa atggctcata cgacttggaa aacgtgcttt agctgttttc aacgaagctt atgatcccaa tggtgtagcg aatacaactg ggacagtatc ttcgatggtc catcgcgtta acaaaggagg taaataa

Details for BabiA.17384.a.B2.GE35908

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML KTPSNSQYDH RIRYVTYNVA DVVQIETVLG VATHIILEEG ERYITHAFGD SDAYAFAHKG RHIFIKPKAE LANTNLIVVT DKRSYKFRLQ FRNDRAGATY ELAFHYPDTN TQKSEANSQH LDIERGFHQG VKGYNLSYTM SGNQDIAPIN AWDNGRITYF KFPANMDMPS IYLVDAEGNE SLIPRTVIGS SNDIIAVHKV NPKWLIRLGK RALAVFNEAY DPNGVANTTG TVSSMVHRVN KGGK

Validated NT Sequence

atcttactca cttatttacc tcctttgtta acgcgatgga ccatcgaaga tactgtccca gttgtattcg ctacaccatt gggatcataa gcttcgttga aaacagctaa agcacgtttt ccaagtcgta tgagccattt gggattgacc ttatgaacgg caataatatc attggaactg cctataacgg tacgtggtat taagctctcg ttaccttcag catctactag gtaaattgat ggcatatcca tgttggcagg aaatttgaaa taggtaatac gcccattgtc ccaagcatta attggagcaa tatcttggtt gccactcatg gtatagctga ggttatagcc ttttacacct tggtgaaaac cgcgttcaat atcaaggtgt tggctgtttg cttctgactt ttgagtattt gtatcaggat aatggaaagc taattcgtag gtggctccag cacgatcatt tcggaactgt aatcgaaatt tatagctacg tttgtctgtt acaacgatga gattcgtatt agctaattct gctttgggtt taataaagat atgtcgtcct ttatgtgcaa aggcataagc atcagaatca ccaaaagcat gcgtgatgta gcgttcaccc tcttcaagga tgatatgtgt tgccacccca agaacggttt caatttgaac cacatcagcc acattatatg ttacatagcg aatgcgatga tcatattgtg aatttgatgg ggttttgagc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca aaaccccatc aaattcacaa tatgatcatc gcattcgcta tgtaacatat aatgtggctg atgtggttca aattgaaacc gttcttgggg tggcaacaca tatcatcctt gaagagggtg aacgctacat cacgcatgct tttggtgatt ctgatgctta tgcctttgca cataaaggac gacatatctt tattaaaccc aaagcagaat tagctaatac gaatctcatc gttgtaacag acaaacgtag ctataaattt cgattacagt tccgaaatga tcgtgctgga gccacctacg aattagcttt ccattatcct gatacaaata ctcaaaagtc agaagcaaac agccaacacc ttgatattga acgcggtttt caccaaggtg taaaaggcta taacctcagc tataccatga gtggcaacca agatattgct ccaattaatg cttgggacaa tgggcgtatt acctatttca aatttcctgc caacatggat atgccatcaa tttacctagt agatgctgaa ggtaacgaga gcttaatacc acgtaccgtt ataggcagtt ccaatgatat tattgccgtt cataaggtca atcccaaatg gctcatacga cttggaaaac gtgctttagc tgttttcaac gaagcttatg atcccaatgg tgtagcgaat acaactggga cagtatcttc gatggtccat cgcgttaaca aaggaggtaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat