SSGCID: Target Page

BabiA.01256.a

TrwD component of type IV secretion system (Trw type IV secretion machinery)

CENTER ID:	BabiA.01256.a
ORGANISM:	Bartonella birtlesii
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BabiA.01256.a.B1.GE35907	full length		1	354

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BabiA.01256.a.B1.PS01666	full length		1	354

External Resources

RESOURCE	REFERENCE ID
UniProt:	D0AAZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMSTASLHVN GIQKDQAVAQ LLQPLDHFLE DPKITELSIN RPCEVWTKNF EGWHVHNVPE LTAAFLQTLI TAIIVYNGMA PRSINYVRLP GGQRGTIIQT PAVIDGTLSF VIRKHSLIVK TLEELNNEGV FDNFADVSFN KPLEKEAENL LSKQDFTRLE PFEVQLLRLK RDKKILEFLE ECVLHKRNII IAGKTGSGKT TFARSLIEKV PIEERMITIE DVHELFLPNH PNHVHMLYGN GAGRVPADEC LDACLRQSPD RIFLAELRGN EAWEYLNSLN TGHPGSITTT HANNALQTFE RCATLIKRSE VGRQLDLEMI KVVLYTTVDV VLFFKDRKLS EIFYDPIFAK SKIV

NT Sequence

gtgatgtcta cagcctcgtt acacgttaat ggaatacaaa aagaccaagc agttgcgcaa cttctgcaac cgcttgatca tttcttagaa gatccaaaaa ttactgaatt atcgataaac cgcccctgtg aagtgtggac aaaaaacttc gagggttggc atgtgcataa tgtaccagaa ttaacggctg cttttttgca aacgcttatt acagccatta tcgtttataa tggtatggcg ccccgaagta ttaattatgt acgcttacct ggtggacaac gtggtaccat tatacaaacg ccagcggtta tcgatggcac actatctttt gtaattcgta agcattccct catagttaag acactagagg aactgaacaa tgaaggtgtg tttgataatt ttgccgatgt cagttttaat aagcctttag aaaaggaagc agaaaatctg ttatcgaagc aagatttcac acgactagag ccatttgaag tacaactttt gcgtctcaaa cgcgataaaa aaattcttga gtttttggaa gaatgcgtgc tgcataaacg caacatcatt attgcaggca aaacaggatc aggaaaaacg acatttgccc gctcgctcat tgaaaaagta cccatagaag aacgcatgat cactattgag gatgtccatg agctttttct gcctaaccat ccaaatcacg ttcatatgct gtatggtaac ggtgcaggtc gtgttccagc tgatgaatgt ttagatgcat gtctgcgtca atcgcctgac cgtatttttc ttgccgaact acgtggcaac gaagcatggg aatatctcaa ctcgttaaac actggacacc caggatcaat tacaacaaca cacgccaaca atgctttgca aacatttgaa cgatgcgcca cattaataaa aagatcagaa gttggtcgtc agcttgattt agaaatgata aaagtagtgc tttacactac agtggatgtg gtgttattct ttaaagatag aaaactctct gaaatcttct atgatcccat ttttgctaaa tcaaagatag tttaa

Details for BabiA.01256.a.B1.GE35907

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	41kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	76

Validated AA Sequence

MAPRSINYVR LPGGQRGTII QTPAVIDGTL SFVIRKHSLI VKTLEELNNE GVFDNFADVS FNKPLEKEAE NLLSKQDFTR LEPFEVQLLR LKRDKKILEF LEECVLHKRN IIIAGKTGSG KTTFARSLIE KVPIEERMIT IEDVHELFLP NHPNHVHMLY GNGAGRVPAD ECLDACLRQS PDRIFLAELR GNEAWEYLNS LNTGHPGSIT TTHANNALQT FERCATLIKR SEVGRQLDLE MIKVVLYTTV DVVLFFKDRK LSEIFYDPIF AKSKIV

Validated NT Sequence

tcacttaaac tatctttgat ttagcaaaaa tgggatcata gaagatttca gagagttttc tatctttaaa gaataacacc acatccactg tagtgtaaag cactactttt atcatttcta aatcaagctg acgaccaact tctgatcttt ttattaatgt ggcgcatcgt tcaaatgttt gcaaagcatt gttggcgtgt gttgttgtaa ttgatcctgg gtgtccagtg tttaacgagt tgagatattc ccatgcttcg ttgccacgta gttcggcaag aaaaatacgg tcaggcgatt gacgcagaca tgcatctaaa cattcatcag ctggaacacg acctgcaccg ttaccataca gcatatgaac gtgatttgga tggttaggca gaaaaagctc atggacatcc tcaatagtga tcatgcgttc ttctatgggt actttttcaa tgagcgagcg ggcaaatgtc gtttttcctg atcctgtttt gcctgcaata atgatgttgc gtttatgcag cacgcattct tccaaaaact caagaatttt tttatcgcgt ttgagacgca aaagttgtac ttcaaatggc tctagtcgtg tgaaatcttg cttcgataac agattttctg cttccttttc taaaggctta ttaaaactga catcggcaaa attatcaaac acaccttcat tgttcagttc ctctagtgtc ttaactatga gggaatgctt acgaattaca aaagatagtg tgccatcgat aaccgctggc gtttgtataa tggtaccacg ttgtccacca ggtaagcgta cataattaat acttcggggc gccataccat tataaacgat aatggctgta ataagcgttt gcaaaaaagc agccgttaat tctggtacat tatgcacatg ccaaccctcg aagttttttg tccacacttc acaggggcgg tttatcgata attcagtaat ttttggatct tctaagaaat gatcaagcgg ttgcagaagt tgcgcaactg cttggtcttt ttgtattcca ttaacgtgta acgaggctgt aga

Expected Protein Sequence

MAHHHHHHMV MSTASLHVNG IQKDQAVAQL LQPLDHFLED PKITELSINR PCEVWTKNFE GWHVHNVPEL TAAFLQTLIT AIIVYNGMAP RSINYVRLPG GQRGTIIQTP AVIDGTLSFV IRKHSLIVKT LEELNNEGVF DNFADVSFNK PLEKEAENLL SKQDFTRLEP FEVQLLRLKR DKKILEFLEE CVLHKRNIII AGKTGSGKTT FARSLIEKVP IEERMITIED VHELFLPNHP NHVHMLYGNG AGRVPADECL DACLRQSPDR IFLAELRGNE AWEYLNSLNT GHPGSITTTH ANNALQTFER CATLIKRSEV GRQLDLEMIK VVLYTTVDVV LFFKDRKLSE IFYDPIFAKS KIV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga tgtctacagc ctcgttacac gttaatggaa tacaaaaaga ccaagcagtt gcgcaacttc tgcaaccgct tgatcatttc ttagaagatc caaaaattac tgaattatcg ataaaccgcc cctgtgaagt gtggacaaaa aacttcgagg gttggcatgt gcataatgta ccagaattaa cggctgcttt tttgcaaacg cttattacag ccattatcgt ttataatggt atggcgcccc gaagtattaa ttatgtacgc ttacctggtg gacaacgtgg taccattata caaacgccag cggttatcga tggcacacta tcttttgtaa ttcgtaagca ttccctcata gttaagacac tagaggaact gaacaatgaa ggtgtgtttg ataattttgc cgatgtcagt tttaataagc ctttagaaaa ggaagcagaa aatctgttat cgaagcaaga tttcacacga ctagagccat ttgaagtaca acttttgcgt ctcaaacgcg ataaaaaaat tcttgagttt ttggaagaat gcgtgctgca taaacgcaac atcattattg caggcaaaac aggatcagga aaaacgacat ttgcccgctc gctcattgaa aaagtaccca tagaagaacg catgatcact attgaggatg tccatgagct ttttctgcct aaccatccaa atcacgttca tatgctgtat ggtaacggtg caggtcgtgt tccagctgat gaatgtttag atgcatgtct gcgtcaatcg cctgaccgta tttttcttgc cgaactacgt ggcaacgaag catgggaata tctcaactcg ttaaacactg gacacccagg atcaattaca acaacacacg ccaacaatgc tttgcaaaca tttgaacgat gcgccacatt aataaaaaga tcagaagttg gtcgtcagct tgatttagaa atgataaaag tagtgcttta cactacagtg gatgtggtgt tattctttaa agatagaaaa ctctctgaaa tcttctatga tcccattttt gctaaatcaa agatagtttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BabiA.01256.a.B1.PS01666

PURIFICATION DATe:	11/1/2012
CONCENTRATION:	38.4mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	76

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTGA TGTCTACAGC CTCGTTACAC GTTAATGGAA TACAAAAAGA CCAAGCAGTT GCGCAACTTC TGCAACCGCT TGATCATTTC TTAGAAGATC CAAAAATTAC TGAATTATCG ATAAACCGCC CCTGTGAAGT GTGGACAAAA AACTTCGAGG GTTGGCATGT GCATAATGTA CCAGAATTAA CGGCTGCTTT TTTGCAAACG CTTATTACAG CCATTATCGT TTATAATGGT ATGGCGCCCC GAAGTATTAA TTATGTACGC TTACCTGGTG GACAACGTGG TACCATTATA CAAACGCCAG CGGTTATCGA TGGCACACTA TCTTTTGTAA TTCGTAAGCA TTCCCTCATA GTTAAGACAC TAGAGGAACT GAACAATGAA GGTGTGTTTG ATAATTTTGC CGATGTCAGT TTTAATAAGC CTTTAGAAAA GGAAGCAGAA AATCTGTTAT CGAAGCAAGA TTTCACACGA CTAGAGCCAT TTGAAGTACA ACTTTTGCGT CTCAAACGCG ATAAAAAAAT TCTTGAGTTT TTGGAAGAAT GCGTGCTGCA TAAACGCAAC ATCATTATTG CAGGCAAAAC AGGATCAGGA AAAACGACAT TTGCCCGCTC GCTCATTGAA AAAGTACCCA TAGAAGAACG CATGATCACT ATTGAGGATG TCCATGAGCT TTTTCTGCCT AACCATCCAA ATCACGTTCA TATGCTGTAT GGTAACGGTG CAGGTCGTGT TCCAGCTGAT GAATGTTTAG ATGCATGTCT GCGTCAATCG CCTGACCGTA TTTTTCTTGC CGAACTACGT GGCAACGAAG CATGGGAATA TCTCAACTCG TTAAACACTG GACACCCAGG ATCAATTACA ACAACACACG CCAACAATGC TTTGCAAACA TTTGAACGAT GCGCCACATT AATAAAAAGA TCAGAAGTTG GTCGTCAGCT TGATTTAGAA ATGATAAAAG TAGTGCTTTA CACTACAGTG GATGTGGTGT TATTCTTTAA AGATAGAAAA CTCTCTGAAA TCTTCTATGA TCCCATTTTT GCTAAATCAA AGATAGTTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT