AsfuA.19525.a

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID: AsfuA.19525.a
ORGANISM: Aspergillus fumigatus
ASSOCIATED DISEASE: Aspergillosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AsfuA.19525.a.B3.GE42497 soluble domain #2 623 1130
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:Afu2g03700
UniProt: A0A0J5PMV7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATSLITRKL RSAEATNDVE PGWLKRQVTG VLQSISSHAC QHPIHTIVVI ALLASTTYVG LLEGSLFDSV RNSRNIAGQV DVDTLLQGSR NLRLGESTSW KWQVKDSLAS QDYTGVNHLA LTTFIFSDSL SKSSSIAPAA TELPIPSNAS AQPVPFTPNL FSPFSHDSSL AFTLPFDQVP QFLKAVQEIP DTSSDEDDGE QKKWIMRAAR GPAYGSGGAI KLWLADAWGS FVDLIKHAET IDIVIMTLGY LSMHLSFVSL FFSMRRLGSN FWLAATVLFS GVFAFLFGLL VTTKLGVPIN VLLLSEGLPF LVVTIGFEKP IILTRAVLTA AADNRGRAGQ ASSSTTKSIQ DSIQTAIKEQ GFEIIRDYCI EIAILIAGAA SGVQGGLRQF CFLAAWILFF DCVLLFTFYT TILCIKLEIN RIKRHITLRK ALEEDGITHR VAENVASSND WPLTESESSN KTSIFGRKLR SSSVRRFKIL MVGGFVLVNV VNLSTIPFRD SSQGAGLPLL SRVSNVFAPT PIDPFKVAEN GLDSIYVSAK SQMMETVVTV IPPIKYKLEY PSVHYAALGE SRTFDIEYTD QFLDAVGGRV IESLLKSIED PIISKWIIAA LTLSIILNGY LFNAARWSIK EPEVAPVPAA PTVAVVEAKK EYPKIDLNPD TPKRSLEECE AFLKEKRAAY LSDEELIELS LRGKIPGYAL EKTMENEDLM SRVDAFTRAV KIRRAVVART PATSAITGSL ECSKLPYKDY NYTLVHGACC ENVIGYLPLP LGVAGPLTID GQSYFIPMAT TEGVLVASTS RGAKAINAGG GAVTVLTGDG MTRGPCVGFP TLARAAAAKV WIDSEEGRSI LTAAFNSTSR FARLQHLKTA LAGTYLYIRF KTTTGDAMGM NMISKGVEKA LHVMATECGF DDMATISVSG NFCTDKKAAA INWIDGRGKS VVAEAIIPGD VVRSVLKSDV NALVELNTSK NLIGSAMAGS VGGFNAHASN IVTAVFLATG QDPAQNVESS SCITTMRNLN GNLQIAVSMP SIEVGTIGGG TILEAQSAML DMLGVRGSHP TNPGDNARQL ARIVAAAVLA GELSLCSALA AGHLVRAHMA HNRSAATTRT STPVSAAVSA ARGLTMSSSE
NT Sequence
atggctacct ctctgattac aaggaagctt cgttcggcag aagccacgaa cgatgtcgaa ccgggatggc taaagcgtca ggtcactggc gttttacagt ccatctcgag tcacgcttgt cagcacccta tacacaccat tgtggttatt gcattgctcg ccagtacgac gtacgtcggt ctccttgaag ggagtttgtt cgacagcgtc aggaactcca ggaatattgc tggtcaagtg gacgtcgata ctctgcttca gggcagccgg aatcttcggt tgggggagag tacatcttgg aaatggcagg tgaaggacag tttagcgagc caggactaca cgggcgtcaa ccatcttgcc ttaacaacct tcatcttctc ggattccctg tccaagtcat cttcaatcgc tcctgccgca acagaattac cgattccttc caatgcctct gctcaaccag tgccatttac acctaacttg ttctcgccct tctcccatga ctcttctctt gcattcacct tgccgtttga tcaggtccca caatttctga aagcggtgca ggagatcccg gacacctcct ccgatgagga cgacggggaa caaaagaaat ggatcatgcg ggccgctcgt ggcccggctt atggctctgg tggtgctatc aagctctggt tggcagatgc gtggggttca ttcgtggatt tgatcaagca cgcggaaact attgatattg tcatcatgac cctgggatac ctttcgatgc accttagctt cgtgtccctc ttcttctcga tgcggcgtct cggctctaac ttctggttgg ctgccacagt cctcttctcg ggcgtttttg catttttgtt tggtcttctg gtcacaacga agcttggcgt cccgatcaat gtccttttgc tgtccgaggg ccttccattc cttgttgtca ctatcggttt cgagaagcca atcattctta ccagggctgt tctcactgcg gcagcggaca acaggggcag agctggtcaa gcttcgtcaa gcaccacaaa gtccattcag gactctattc agaccgcgat caaggaacaa ggttttgaga ttatccgcga ttactgcatc gagatcgcca ttcttattgc cggggccgct tctggagtcc agggtgggtt gcgacaattc tgttttctcg cggcgtggat cttgtttttc gactgcgttc tacttttcac tttctacacc accatcctct gcatcaaact cgaaattaac cggatcaagc gccacatcac tcttcgcaaa gctctggaag aagatggtat cacccatcgg gtggccgaga atgtcgcttc cagtaatgat tggcctctga ccgaatctga gagcagcaac aagactagca tctttgggag aaagctccgc tccagcagtg ttcgcagatt caagattttg atggtaggag gttttgtctt ggtcaatgtg gtgaatctat ccaccatccc attccgggac tcaagccagg gtgctggcct acctctattg tctcgtgtct ctaatgtgtt tgcgcctacg cccattgacc ctttcaaggt cgcagaaaat gggcttgact caatctatgt ctctgcaaag agccagatga tggagacggt tgtgacagtc atccctccaa tcaagtacaa gcttgaatat ccttctgtac actacgccgc tttgggtgaa agccgcactt tcgatatcga gtacaccgat cagttcctcg acgccgttgg cgggcgtgtc atcgagagtc ttctcaagag tatcgaagat cctatcatca gcaagtggat cattgctgct ctgaccctga gcattatctt gaacggctac ttgttcaatg ccgctcgctg gagcattaag gagcccgaag ttgcccctgt ccctgccgcc cccacggtgg ctgtggtgga agcgaagaag gaatacccta agatcgactt gaaccccgac actcccaaga ggagtttaga agaatgtgag gccttcttga aagagaagcg agccgcttac ttgagcgatg aagaattgat tgaactttcc ctgcgaggca agattcctgg ctatgcgttg gaaaagacca tggagaacga ggacctcatg agccgtgtgg atgcgttcac tagagcggtc aagatccgtc gagctgttgt tgctcgaact cctgccactt ccgctatcac aggctctttg gaatgttcaa agctcccata caaggattac aactatacgc ttgtgcacgg tgcctgctgt gagaatgtca ttggatatct gccattgcct ttgggagtgg ctggcccctt gaccattgat ggccagagct actttattcc catggctacc acggaaggtg tgctggtggc cagcacaagt cgaggcgcca aggcgattaa cgcaggtggt ggcgcggtca ctgtcctgac tggcgacggc atgactcgtg gcccttgtgt cggtttccct actcttgcac gcgcggcggc tgctaaggtc tggattgatt cagaagaggg tcgaagcatt ttgactgcgg cttttaactc taccagtcgg tttgcccgcc ttcaacatct taagacggct ctcgctggca cttatcttta catccggttc aagaccacaa caggtgacgc catggggatg aatatgatct ctaaaggtgt cgaaaaggcg ctccacgtta tggcgactga gtgcggcttt gacgacatgg ctaccatttc cgtttctggt aacttctgta ctgacaaaaa ggctgctgct attaactgga ttgacggtcg tggcaagtcc gtggtggctg aggccatcat tcctggtgat gttgtccgga gcgtgctcaa gagtgacgta aatgccctgg tcgagctcaa taccagtaag aacttgattg gaagtgctat ggccggaagc gtgggtgggt tcaacgctca cgcatcgaac attgtcactg ctgttttctt ggcaacgggt caggatcctg cgcagaatgt cgagagcagc agctgtatca cgaccatgag aaacctcaat ggcaacctcc agatcgctgt gtctatgcct tccatcgaag ttggcacaat cggcggtggc acgattctcg aagcacaatc tgccatgctt gatatgctag gtgtccgtgg ttcccaccct accaatccag gtgacaacgc tcggcaactt gctcgaattg tcgctgccgc tgttcttgcg ggagagctga gcttgtgctc tgcacttgcc gccggccacc ttgtcagagc gcacatggcg cacaaccgca gtgctgctac gacacggact tctacccccg tctcagcagc cgtgagtgct gctcggggat tgacgatgtc ctcctcggag tga
Details for AsfuA.19525.a.B3.GE42497
HARVESTED ON: data unavailable
SEQUENCED ON: 7/10/2018
EXPECTED MW: 54kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMN AARWSIKEPE VAPVPAAPTV AVVEAKKEYP KIDLNPDTPK RSLEECEAFL KEKRAAYLSD EELIELSLRG KIPGYALEKT MENEDLMSRV DAFTRAVKIR RAVVARTPAT SAITGSLECS KLPYKDYNYT LVHGACCENV IGYLPLPLGV AGPLTIDGQS YFIPMATTEG VLVASTSRGA KAINAGGGAV TVLTGDGMTR GPCVGFPTLA RAAAAKVWID SEEGRSILTA AFNSTSRFAR LQHLKTALAG TYLYIRFKTT TGDAMGMNMI SKGVEKALHV MATECGFDDM ATISVSGNFC TDKKAAAINW IDGRGKSVVA EAIIPGDVVR SVLKSDVNAL VELNTSKNLI GSAMAGSVGG FNAHASNIVT AVFLATGQDP AQNVESSSCI TTMRK
Validated NT Sequence
atcttactca cttactccga ggaggacatc gtcaatcccc gagcagcact cacggctgct gagacggggg tagaagtccg tgtcgtagca gcactgcggt tgtgcgccat gtgcgctctg acaaggtggc cggcggcaag tgcagagcac aagctcagct ctcccgcaag aacagcggca gcgacaattc gagcaagttg ccgagcgttg tcacctggat tggtagggtg ggaaccacgg acacctagca tatcaagcat ggcagattgt gcttcgagaa tcgtgccacc gccgattgtg ccaacttcga tggaaggcat agacacagcg atctggaggt tgccattgag gcttagaaaa ttggctagtt agtactcgag caacgaaaac aaattgagaa tgtatgacaa ctcactttct catggtcgtg atacagctgc tgctctcgac attctgcgca ggatcctgac ccgttgccaa gaaaacagca gtgacaatgt tcgatgcgtg agcgttgaac ccacccacgc ttccggccat agcacttcca atcaagttct tactggtatt gagctcgacc agggcattta cgtcactctt gagcacgctc cggacaacat caccaggaat gatggcctca gccaccacgg acttgccacg accgtcaatc cagttaatag cagcagcctt tttgtcagta cagaagttac cagaaacgga aatggtagcc atgtcgtcaa agccgcactc agtcgccata acgtggagcg ccttttcgac acctttagag atcatattca tccccatggc gtcacctgtt gtggtcttga accggatgta aagataagtg ccagcgagag ccgtcttaag atgttgaagg cgggcaaacc gactggtaga gttaaaagcc gcagtcaaaa tgcttcgacc ctcttctgaa tcaatccaga ccttagcagc cgccgcgcgt gcaagagtag ggaaaccgac acaagggcca cgagtcatgc cgtcgccagt caggacagtg accgcgccac cacctgcgtt aatcgccttg gcgcctcgac ttgtgctggc caccagcaca ccttccgtgg tagccatggg aataaagtag ctctggccat caatggtcaa ggggccagcc actcccaaag gcaatggcag atatccaatg acattctcac agcaggcacc gtgcacaagg gtatagttgt aatccttgta tgggagcttt gaacattcca aagagcctgt gatagcggaa gtggcaggag ttcgagcaac aacagctcga cggatcttga ccgctctagt gaacgcatcc acacggctca tgaggtcctc gttctccatg gtcttttcca acgcatagcc aggaatcttg cctcgcaggg aaagttcaat caattcttca tcgctcaagt aagcggctcg cttctctttc aagaaggcct cacattcttc taaactcctc ttgggagtgt cggggttcaa gtcgatctta gggtattcct tcttcgcttc caccacagcc accgtggggg cggcagggac aggggcaact tcgggctcct taatgctcca gcgagcggca ttcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHNA ARWSIKEPEV APVPAAPTVA VVEAKKEYPK IDLNPDTPKR SLEECEAFLK EKRAAYLSDE ELIELSLRGK IPGYALEKTM ENEDLMSRVD AFTRAVKIRR AVVARTPATS AITGSLECSK LPYKDYNYTL VHGACCENVI GYLPLPLGVA GPLTIDGQSY FIPMATTEGV LVASTSRGAK AINAGGGAVT VLTGDGMTRG PCVGFPTLAR AAAAKVWIDS EEGRSILTAA FNSTSRFARL QHLKTALAGT YLYIRFKTTT GDAMGMNMIS KGVEKALHVM ATECGFDDMA TISVSGNFCT DKKAAAINWI DGRGKSVVAE AIIPGDVVRS VLKSDVNALV ELNTSKNLIG SAMAGSVGGF NAHASNIVTA VFLATGQDPA QNVESSSCIT TMRNLNGNLQ IAVSMPSIEV GTIGGGTILE AQSAMLDMLG VRGSHPTNPG DNARQLARIV AAAVLAGELS LCSALAAGHL VRAHMAHNRS AATTRTSTPV SAAVSAARGL TMSSSE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatgccg ctcgctggag cattaaggag cccgaagttg cccctgtccc tgccgccccc acggtggctg tggtggaagc gaagaaggaa taccctaaga tcgacttgaa ccccgacact cccaagagga gtttagaaga atgtgaggcc ttcttgaaag agaagcgagc cgcttacttg agcgatgaag aattgattga actttccctg cgaggcaaga ttcctggcta tgcgttggaa aagaccatgg agaacgagga cctcatgagc cgtgtggatg cgttcactag agcggtcaag atccgtcgag ctgttgttgc tcgaactcct gccacttccg ctatcacagg ctctttggaa tgttcaaagc tcccatacaa ggattacaac tatacgcttg tgcacggtgc ctgctgtgag aatgtcattg gatatctgcc attgcctttg ggagtggctg gccccttgac cattgatggc cagagctact ttattcccat ggctaccacg gaaggtgtgc tggtggccag cacaagtcga ggcgccaagg cgattaacgc aggtggtggc gcggtcactg tcctgactgg cgacggcatg actcgtggcc cttgtgtcgg tttccctact cttgcacgcg cggcggctgc taaggtctgg attgattcag aagagggtcg aagcattttg actgcggctt ttaactctac cagtcggttt gcccgccttc aacatcttaa gacggctctc gctggcactt atctttacat ccggttcaag accacaacag gtgacgccat ggggatgaat atgatctcta aaggtgtcga aaaggcgctc cacgttatgg cgactgagtg cggctttgac gacatggcta ccatttccgt ttctggtaac ttctgtactg acaaaaaggc tgctgctatt aactggattg acggtcgtgg caagtccgtg gtggctgagg ccatcattcc tggtgatgtt gtccggagcg tgctcaagag tgacgtaaat gccctggtcg agctcaatac cagtaagaac ttgattggaa gtgctatggc cggaagcgtg ggtgggttca acgctcacgc atcgaacatt gtcactgctg ttttcttggc aacgggtcag gatcctgcgc agaatgtcga gagcagcagc tgtatcacga ccatgagaaa cctcaatggc aacctccaga tcgctgtgtc tatgccttcc atcgaagttg gcacaatcgg cggtggcacg attctcgaag cacaatctgc catgcttgat atgctaggtg tccgtggttc ccaccctacc aatccaggtg acaacgctcg gcaacttgct cgaattgtcg ctgccgctgt tcttgcggga gagctgagct tgtgctctgc acttgccgcc ggccaccttg tcagagcgca catggcgcac aaccgcagtg ctgctacgac acggacttct acccccgtct cagcagccgt gagtgctgct cggggattga cgatgtcctc ctcggagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at