SSGCID: Target Page

AsfuA.19172.a

Pantothenate kinase, putative (PanK)

CENTER ID:	AsfuA.19172.a
ORGANISM:	Aspergillus fumigatus
ASSOCIATED DISEASE:	Aspergillosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AsfuA.19172.a.B11.GE42496	full length		1	456

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AsfuA.19172.a.B11.PW38533	full length		1	456

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:Afu3g07180
UniProt:	Q4WWV3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSMSSPKSNS VGPDAVAPKR VTNSFTERTG RLERAITNPG AVKINVKGAF IVDEEPRSKS PASSTDGVHY ESQDIRLPHH TGLVSHVAVD IGGSLAKLVY FTKELDSPDN GGRLNFINFE THRIDLCIDF IRQLKDEHER LNGPSRDELC VVATGGGAYK YYDKLKETLK VNIIREDEME CLITGLDFFI TEIPNEVFTY SEAEPMQFAE ARPDVYPYLL VNIGSGVSMI KVSGPKQFER VGGTHLGGGT FWGLMSLLTG TRNFDDMLAM ADRGDNSGVD MLVGDIYGMD YSKIGLKSTA IASTFGKVLR LQNAAERHAE DGEGLCNGGP EQQNGEVKFR HEDMSRSLLY AISNNIGQIA YLQSEKHQVK HIYFGGSFIR GHHQTMNTLS YAIRFWSKGE KQAYFLRHEG YLGAVGAFLR RQPENWGRRN SIHDASSAIQ AAKEAIPKKD EGLSST

NT Sequence

atgtccatgt cctccccaaa atccaattcg gtcggcccag atgcggtcgc acctaagcgt gtgaccaact cattcacaga gcgaaccggc cggcttgaac gagctatcac caaccctgga gctgtcaaga tcaacgtcaa aggtgctttc attgtcgacg aagagccccg gtcgaaaagt cccgcgtcat cgacggacgg cgttcactac gagagccagg atattcgtct acctcatcat actggcctcg tgagccatgt cgctgtcgat attggaggct cgctggcgaa gctggtatat tttaccaagg aattggactc tcctgacaac ggtggtcgcc tgaatttcat caactttgag actcaccgga tcgatttgtg cattgacttc atccggcaat taaaagatga acatgagagg ctcaatggcc cctctaggga tgagttgtgt gtcgtcgcta caggaggagg tgcctacaag tactacgata agctgaaaga gacgctgaaa gtgaacatca tacgggaaga cgaaatggaa tgcctcataa caggacttga cttctttatt accgagattc cgaatgaagt gtttacatac agcgaagccg agccgatgca gtttgccgag gcccgaccgg atgtttaccc gtacctatta gtcaacattg gttcaggagt atcaatgatc aaagtatctg gcccaaagca atttgagcgt gtcggtggta ctcaccttgg cggtggcaca ttctggggcc tgatgtcgtt gctgaccggt actcggaact ttgacgacat gctggctatg gcggaccggg gcgacaacag tggggtggac atgttagttg gcgatatcta tggcatggac tatagcaaga tcggacttaa gagcactgcc attgctagta catttggcaa ggtccttcgt ttacaaaatg ctgccgagcg acatgcggag gatggggaag gtctctgcaa tggcggccct gagcaacaaa atggtgaagt caagtttcgt catgaagaca tgagccgaag cttgctgtat gctatcagta acaacattgg gcagattgcc tacttacaat ctgagaagca ccaggttaag cacatatact ttggaggctc gtttattcgg gggcatcacc agacgatgaa tacattgtcg tatgccatcc gattctggtc taagggtgag aaacaagcct acttcctacg ccatgaaggc tacctcggcg ctgttggtgc atttctcagg aggcaaccag agaactgggg tcgcagaaat agtattcatg acgcatcttc cgcaattcag gcggcaaagg aagccatccc caagaaagat gaagggttga gctcaacgta g

Details for AsfuA.19172.a.B11.GE42496

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS MSSPKSNSVG PDAVAPKRVT NSFTERTGRL ERAITNPGAV KINVKGAFIV DEEPRSKSPA SSTDGVHYES QDIRLPHHTG LVSHVAVDIG GSLAKLVYFT KELDSPDNGG RLNFINFETH RIDLCIDFIR QLKDEHERLN GPSRDELCVV ATGGGAYKYY DKLKETLKVN IIREDEMECL ITGLDFFITE IPNEVFTYSE AEPMQFAEAR PDVYPYLLVN IGSGVSMIKV SGPKQFERVG GTHLGGGTFW GLMSLLTGTR NFDDMLAMAD RGDNSGVDML VGDIYGMDYS KIGLKSTAIA STFGKVLRLQ NAAERHAEDG EGLCNGGPEQ QNGEVKFRHE DMSRSLLYAI SNNIGQIAYL QSEKHQVKHI YFGGSFIRGH HQTMNTLSYA IRFWSKGEKQ AYFLRHEGYL GAVGAFLRRQ PENWGRRNSI HDASSAIQAA KEAIPKKDEG LSST

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca tgagctcgcc gaaaagcaac tcggtgggtc cggacgcggt agctccgaaa cgtgtaacta atagcttcac cgaacgtact ggccgtctgg aacgcgctat cactaacccg ggtgcagtca aaattaacgt taagggtgct ttcatcgtgg acgaagagcc gcgtagcaag tcaccggctt ctagcaccga tggcgtgcat tacgaatcac aggacatccg tctgccgcac cacactgggc tggtgagcca cgtcgctgtt gatatcgggg gtagcctggc taagctggtg tatttcacga aagaactgga cagcccagat aacggtggtc gtctgaactt catcaacttt gagactcacc gcatcgacct gtgcatcgat ttcatccgtc agctgaagga cgagcacgaa cgtctgaacg gcccttctcg tgatgaactg tgtgtcgttg ctactggtgg cggcgcttac aaatactacg ataagttgaa agagactctg aaggttaaca ttatccgcga agacgaaatg gagtgcctga tcacgggcct ggacttcttc atcaccgaga ttccgaacga agtgttcacc tactcagaag ctgagccgat gcagttcgca gaagcccgtc cggatgttta cccgtatctg ctggtgaaca tcggcagcgg cgtttcgatg atcaaagtgt ctggtccgaa acagttcgaa cgcgttggtg ggactcacct ggggggtggt accttttggg gtctgatgtc tctgctgacc ggcacccgca acttcgatga tatgctggct atggctgatc gcggtgacaa ctccggcgta gacatgctgg tgggtgacat ttatggtatg gattattcta agatcggcct gaagtccaca gcgatcgctt ccaccttcgg taaagtgctg cgcctgcaga acgcagcgga acgccatgca gaggatggcg aaggtctgtg caacggtggt ccggaacaac agaacggcga agtaaaattc cgtcacgaag acatgagccg ctctctgctg tacgccattt ccaacaatat cggtcagatt gcgtacctgc agtctgagaa acaccaggtt aaacacatct acttcggtgg ttcgttcatc cgtggtcatc accagaccat gaacactctg agttacgcta ttcgtttctg gtctaaaggc gaaaaacagg catatttcct gcgccacgaa ggttacctcg gcgctgtggg tgcgttcctg cgtcgtcagc cggaaaactg gggtcgccgc aacagcatcc atgatgcgtc ctctgcaatc caggctgcga aagaagctat tcctaagaaa gatgaaggcc tgtcctcaac ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for AsfuA.19172.a.B11.PW38533

PURIFICATION DATe:	10/1/2018
CONCENTRATION:	32.35mg/ml
OBSERVED MW:	51kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	17
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCA TGAGCTCGCC GAAAAGCAAC TCGGTGGGTC CGGACGCGGT AGCTCCGAAA CGTGTAACTA ATAGCTTCAC CGAACGTACT GGCCGTCTGG AACGCGCTAT CACTAACCCG GGTGCAGTCA AAATTAACGT TAAGGGTGCT TTCATCGTGG ACGAAGAGCC GCGTAGCAAG TCACCGGCTT CTAGCACCGA TGGCGTGCAT TACGAATCAC AGGACATCCG TCTGCCGCAC CACACTGGGC TGGTGAGCCA CGTCGCTGTT GATATCGGGG GTAGCCTGGC TAAGCTGGTG TATTTCACGA AAGAACTGGA CAGCCCAGAT AACGGTGGTC GTCTGAACTT CATCAACTTT GAGACTCACC GCATCGACCT GTGCATCGAT TTCATCCGTC AGCTGAAGGA CGAGCACGAA CGTCTGAACG GCCCTTCTCG TGATGAACTG TGTGTCGTTG CTACTGGTGG CGGCGCTTAC AAATACTACG ATAAGTTGAA AGAGACTCTG AAGGTTAACA TTATCCGCGA AGACGAAATG GAGTGCCTGA TCACGGGCCT GGACTTCTTC ATCACCGAGA TTCCGAACGA AGTGTTCACC TACTCAGAAG CTGAGCCGAT GCAGTTCGCA GAAGCCCGTC CGGATGTTTA CCCGTATCTG CTGGTGAACA TCGGCAGCGG CGTTTCGATG ATCAAAGTGT CTGGTCCGAA ACAGTTCGAA CGCGTTGGTG GGACTCACCT GGGGGGTGGT ACCTTTTGGG GTCTGATGTC TCTGCTGACC GGCACCCGCA ACTTCGATGA TATGCTGGCT ATGGCTGATC GCGGTGACAA CTCCGGCGTA GACATGCTGG TGGGTGACAT TTATGGTATG GATTATTCTA AGATCGGCCT GAAGTCCACA GCGATCGCTT CCACCTTCGG TAAAGTGCTG CGCCTGCAGA ACGCAGCGGA ACGCCATGCA GAGGATGGCG AAGGTCTGTG CAACGGTGGT CCGGAACAAC AGAACGGCGA AGTAAAATTC CGTCACGAAG ACATGAGCCG CTCTCTGCTG TACGCCATTT CCAACAATAT CGGTCAGATT GCGTACCTGC AGTCTGAGAA ACACCAGGTT AAACACATCT ACTTCGGTGG TTCGTTCATC CGTGGTCATC ACCAGACCAT GAACACTCTG AGTTACGCTA TTCGTTTCTG GTCTAAAGGC GAAAAACAGG CATATTTCCT GCGCCACGAA GGTTACCTCG GCGCTGTGGG TGCGTTCCTG CGTCGTCAGC CGGAAAACTG GGGTCGCCGC AACAGCATCC ATGATGCGTC CTCTGCAATC CAGGCTGCGA AAGAAGCTAT TCCTAAGAAA GATGAAGGCC TGTCCTCAAC CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT