AsfuA.01011.a

Calcineurin Ca2+-binding regulatory subunit CnaB

CENTER ID:	AsfuA.01011.a
ORGANISM:	Aspergillus fumigatus
ASSOCIATED DISEASE:	Aspergillosis
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AsfuA.01011.a.FX11.GE00469	full length		21	194

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:Afu6g04540
RefSeq:	XP_747624.1
UniProt:	Q4WDF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEQPSEPNNA AMYDARRRRA SVGTSQLLDN IVSASNFDRD EVDRLRKRFM KLDKDSSGTI DRDEFLSLPQ VSSNPLATRM IAIFDEDGGG DVDFQEFVSG LSAFSSKGNK EEKLRFAFKV YDIDRDGYIS NGELFIVLKM MVGNNLKDVQ LQQIVDKTIM EADKDRDGKI SFEEFTEMVE NTDVSLSMTL SMF

NT Sequence

atggagcagc ccagtgaacc aaataatgcc gctatgtatg atgcacgcag gcgtagagca tctgttggga catcacagtt attagacaac attgtctcgg cttcgaattt cgacagagat gaggtggaca ggctccgcaa gcgcttcatg aaacttgaca aggatagctc cggcaccatc gatcgtgacg agttcctttc cctcccacaa gtgtcctcaa acccgctcgc aacgaggatg atcgcgatct tcgacgaaga cggcggcggg gatgttgact tccaggagtt tgtgtcgggg ctgtcggctt tcagctccaa gggtaacaaa gaggagaagc tccggttcgc gttcaaggtc tacgacattg accgggacgg ctatatctcc aacggcgagc tgttcatcgt gcttaagatg atggtgggca ataacctgaa ggacgtgcag ctgcagcaga tcgtggataa gacgatcatg gaagcggata aggaccgcga cggcaagatc agcttcgagg agttcacgga gatggtggag aataccgatg tcagcttgag catgacactg agtatgttct ag

Details for AsfuA.01011.a.FX11.GE00469

HARVESTED ON:	8/10/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	20kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Data Unavailable
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MSVGTSQLLD NIVSASNFDR DEVDRLRKRF MKLDKDSSGT IDRDEFLSLP QVSSNPLATR MIAIFDEDGG GDVDFQEFVS GLSAFSSKGN KEEKLRFAFK VYDIDRDGYI SNGELFIVLK MMVGNNLKDV QLQQIVDKTI MEADKDRDGK ISFEEFTEMV ENTDVSLSMT LSMF

Full NT Sequence (Expression Vector + Insert)

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg cggatccatg tctgtaggca cttctcagct cctggacaac atagtttctg ctagcaactt tgaccgtgac gaggttgatc gtctgcgtaa acgtttcatg aagctcgaca aggactcctc tggcaccatt gaccgtgatg aatttctgag ccttcctcag gtatcttcca acccactggc aactcgtatg atagctatct tcgatgagga cggcggtggc gacgtagatt tccaagaatt tgtttccggt ctgagcgcat tctcctctaa gggcaataag gaagagaagt tacgctttgc atttaaggtt tatgatatcg atcgtgacgg ctatatcagc aacggcgagc tgttcatagt tctgaagatg atggtaggta acaacctcaa ggacgttcag ctgcagcaga ttgttgacaa gactataatg gaggcagaca aggatcgtga cggtaaaatc tcttttgaag aatttaccga aatggttgag aatactgacg tgtccctgag catgacctta tccatgttct gataagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctaggag atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgg