AsfuA.00629.a
Acetyl-coenzyme A synthetase (EC 6.2.1.1)
| CENTER ID: | AsfuA.00629.a |
| ORGANISM: | Aspergillus fumigatus |
| ASSOCIATED DISEASE: | Aspergillosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AsfuA.00629.a.FS11.GE00454 | full length | 2 | 671 | ||
| AsfuA.00629.a.HA11.GE00474 | full length | 40 | 667 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AsfuA.00629.a.FS11.PD00398 | full length | 2 | 671 | ||
| AsfuA.00629.a.HA11.PD00404 | full length | 40 | 667 | ||
| AsfuA.00629.a.FS11.PD00415 | full length | 2 | 671 |
Structures
7KDN
DEPOSITED: 10/9/2020
DETERMINATION: XRay
CLONE: AsfuA.00629.a.FS11.GE00454
PROTEIN: AsfuA.00629.a.FS11.PD00486
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:Afu4g11080 |
| RefSeq: | XP_751720.1 |
| UniProt: | Q4WQ02 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDGDVKPAK PAVVLEANDV DTFHVPKAFY EKHPSKTHLK DLDEYKKLYD ESIRSPDTFW ARMARELLTF DKDFQTTHIG SLENGDNAWF VEGRLNASFN CVDRHAIKNP NKVAIIYEAD EPNEGRIITY GELLREVSRV AWVLKQRGVK KGDTVAIYLP MIPEAVVAFL ACARIGAIHS VVFAGFSSDS LRDRVLDAGS KVVITTDEGK RGGKVIGTKR IVDEALKQCP DVTSVLVYKR TGAEVPWTNG RDIWWHEEVE KYPCYVAPES MSSEDPLFLL YTSGSTGKPK GVMHTTAGYL LGAAMTGKYV FDIHDDDRFF CGGDVGWITG HTYVVYAPLL LGCSTVVFES TPAYPNFSRY WDVIEKHKVT QFYVAPTALR LLKRAGDEHI HHKMEHLRIL GSVGEPIAAE VWKWYFEKVG KEEAHICDTY WQTETGSHVI SPLGGITSTK PGSASLPFFG IEPAIIDPVS GEEISGNDVE GVLAFKQPWP SMARTVWGAH KRYMDTYLNV YKGYYFTGDG AGRDHDGYYW IRGRVDDVVN VSGHRLSTAE IEAALIEHHQ VAEAAVVGIA DELTGQAVNA FVSLKDGNET TEQVRKDLVM QVRKSIGPFA APKAVFVVED LPKTRSGKIM RRILRKILSG EEDSLGDTST LSDPSVVNKI IETVHAARAK
NT Sequence
atgtctgacg gcgacgttaa gcccgcaaag ccggccgtgg tgctcgaggc caacgatgtc gacactttcc acgtccccaa agctttctat gagaagcacc cttcgaagac tcatctgaaa gatttggacg agtataagaa actatacgac gagtctatcc gtagccctga cacgttctgg gcccgcatgg cccgtgagct gctcactttc gacaaagact tccaaaccac gcacatcggg tcgctcgaga atggagacaa tgcctggttc gtcgaaggtc gcttgaatgc ctcgttcaac tgcgtggatc gtcatgccat caagaaccct aataaggtcg cgatcatcta cgaggccgat gagcctaatg agggccgcat cattacctac ggcgagctcc tccgcgaagt ctcccgcgtt gcctgggtcc tgaagcagcg gggtgtcaag aagggtgaca ctgtcgcgat ctaccttccc atgattcctg aagccgttgt ggcttttctc gcctgtgccc gtatcggtgc cattcactcc gttgtcttcg ctggtttctc ctcggactcc ctgcgtgacc gtgtccttga tgctggatcc aaggtcgtta tcaccacgga cgagggtaag cgtggtggca aggtgattgg caccaagcgc attgtcgatg aggccctcaa gcagtgcccc gatgtgacca gtgtgctggt ctacaagcgc actggtgccg aagtcccttg gaccaatggg cgtgacattt ggtggcatga ggaggtcgag aagtacccct gctatgtcgc tcctgagtcg atgagctccg aggaccccct cttcctcctc tacacctctg gttcgactgg taagcccaag ggtgttatgc acactactgc cggctacctc cttggtgccg cgatgaccgg caagtacgtc tttgacattc atgacgacga tcgcttcttc tgcggtggtg acgttggctg gatcaccgga cacacctacg tcgtctatgc gcctcttctt cttggttgct ctaccgtcgt tttcgaaagc actcccgcct accccaactt ctcccgctac tgggatgtca ttgagaagca caaggtcacc cagttctatg tcgcccccac agctctgcgt ctgctgaagc gtgccggtga tgagcacatc caccacaaga tggagcacct gcgtatcctg ggctccgtcg gtgaaccaat tgccgctgag gtctggaagt ggtactttga gaaggtcgga aaggaggaag ctcacatttg cgacacctac tggcaaactg agaccggctc gcacgtcatc agtcccctcg gtggaattac ttccaccaag ccaggcagtg catccttgcc cttcttcggc attgaacccg ctatcatcga ccccgtctcc ggcgaggaga tctctggcaa cgatgtcgag ggtgttcttg ctttcaagca gccttggcct agcatggccc ggaccgtgtg gggtgcccac aagcgctata tggacacata cctgaacgtc tacaagggct actacttcac tggagatggc gctggccgtg accacgacgg atattactgg attcgcggtc gtgtcgacga cgtggtcaat gtctctggac accgtttgtc tactgctgag atcgaggccg ctcttattga gcaccaccag gttgccgaag cggctgtcgt cggtattgcc gatgaactca ctggccaagc tgtcaacgcc ttcgtttctc tcaaggacgg caacgagacc accgaacaag tccgcaagga tcttgtgatg caagttcgca agtcaatcgg tccatttgcc gcacccaagg cggttttcgt cgtagaagac cttcccaaga cccgcagtgg caagattatg cgccgtatcc tacggaagat tctgagcggt gaagaagata gcttgggtga tacctcaacc ttgtcggatc cttccgtggt caacaagatc attgagactg ttcatgctgc ccgagccaag tag
Details for AsfuA.00629.a.FS11.GE00454
| HARVESTED ON: | 8/7/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 76kDa |
| OBSERVED MW: | 76kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | Data not available |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGSDGD VKPAKPAVVL EANDVDTFHV PKAFYEKHPS KTHLKDLDEY KKLYDESIRS PDTFWARMAR ELLTFDKDFQ TTHIGSLENG DNAWFVEGRL NASFNCVDRH AIKNPNKVAI IYEADEPNEG RIITYGELLR EVSRVAWVLK QRGVKKGDTV AIYLPMIPEA VVAFLACARI GAIHSVVFAG FSSDSLRDRV LDAGSKVVIT TDEGKRGGKV IGTKRIVDEA LKQCPDVTSV LVYKRTGAEV PWTNGRDIWW HEEVEKYPCY VAPESMSSED PLFLLYTSGS TGKPKGVMHT TAGYLLGAAM TGKYVFDIHD DDRFFCGGDV GWITGHTYVV YAPLLLGCST VVFESTPAYP NFSRYWDVIE KHKVTQFYVA PTALRLLKRA GDEHIHHKME HLRILGSVGE PIAAEVWKWY FEKVGKEEAH ICDTYWQTET GSHVISPLGG ITSTKPGSAS LPFFGIEPAI IDPVSGEEIS GNDVEGVLAF KQPWPSMART VWGAHKRYMD TYLNVYKGYY FTGDGAGRDH DGYYWIRGRV DDVVNVSGHR LSTAEIEAAL IEHHQVAEAA VVGIADELTG QAVNAFVSLK DGNETTEQVR KDLVMQVRKS IGPFAAPKAV FVVEDLPKTR SGKIMRRILR KILSGEEDSL GDTSTLSDPS VVNKIIETVH AARAK
Full NT Sequence (Expression Vector + Insert)
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aacttttaga gaccaaggag gtaaaaaatg caccatcacc atcatcacca ccatgaaaat ctgtatttcc agggtagcga cggcgacgtg aagccagcca aaccggcagt tgtgctggaa gcgaatgacg tcgatacctt ccacgttccg aaagcatttt acgagaaaca tccgagcaag acgcacctga aagacctgga cgagtacaaa aagttatatg atgagtccat ccgttcgccg gatacctttt gggcacgtat ggctcgcgag ctgctgacgt ttgataaaga cttccaaacc acccacatcg gtagcttgga gaacggtgat aatgcatggt tcgttgaggg tcgtctgaac gccagcttta actgtgtcga ccgtcatgca atcaagaacc cgaacaaagt cgcgatcatt tacgaggctg acgagccgaa cgagggccgc atcatcactt atggtgagct gctgcgtgag gttagccgcg tggcgtgggt cctgaaacag cgtggtgtca aaaagggcga tacggttgcg atttacttgc cgatgattcc ggaagccgtt gtggcgttcc tggcgtgtgc acgcatcggt gcgattcact ctgtggtttt cgcgggtttt agcagcgata gcctgcgcga ccgcgtgctg gatgctggta gcaaagttgt tatcacgacc gacgagggca agcgcggtgg caaggttatc ggtaccaagc gtattgttga cgaagcgttg aaacagtgcc ctgacgtcac gagcgtgctg gtgtacaagc gtacgggcgc agaagtgccg tggacgaatg gtcgcgatat ttggtggcac gaagaggtcg agaagtatcc atgctacgtg gcaccagagt ccatgtcctc cgaggacccg ctgtttctgc tgtacacctc cggtagcact ggtaagccga agggtgttat gcacaccacc gcgggttatt tgctgggtgc ggcgatgacc ggtaaatacg tctttgacat tcacgacgac gatcgcttct tctgcggtgg cgatgtcggc tggatcacgg gccacaccta cgtcgtctat gccccgctgc tgctgggttg cagcaccgtg gtctttgaaa gcaccccggc atacccgaat ttcagccgtt actgggacgt gattgaaaag cacaaagtca cgcagttcta tgtggcgccg acggcgctcc gtctgctgaa gcgtgcgggc gacgagcaca tccatcacaa aatggagcac ttgcgtatcc tgggtagcgt tggcgagcct attgctgcag aggtgtggaa atggtatttt gaaaaagtcg gcaaagaaga ggcacacatc tgtgatactt actggcagac ggaaaccggt tcgcatgtaa ttagcccgct gggtggcatc accagcacta aaccgggcag cgcgagcctg cctttcttcg gtattgagcc ggctatcatt gatccggtga gcggcgaaga aatcagcggt aacgacgtcg agggcgtcct ggcctttaaa cagccgtggc cgagcatggc gcgcacggtg tggggtgcgc ataagcgtta catggatacc tatctgaatg tatacaaggg ctactatttc accggtgacg gtgcgggtcg tgatcatgac ggttactatt ggattcgtgg ccgtgtggat gatgttgtta atgtgagcgg tcaccgcctt agcacggccg aaattgaagc tgcgctgatt gagcatcatc aagtggccga ggcagccgtt gtcggcatcg ccgacgaact gaccggccaa gccgttaatg cgtttgtgtc gctgaaggac ggtaacgaaa cgaccgaaca agttcgtaaa gacctggtca tgcaagtccg taagtctatc ggcccgttcg cggcaccgaa agctgttttt gttgtggaag atttgccgaa aacccgtagc ggtaagatta tgcgtcgcat tctgcgtaag attctgtccg gtgaagagga cagcctgggt gatacgagca ccctgtctga tccgagcgtg gtgaacaaga tcatcgaaac cgttcacgcg gcacgtgcca agtaatgagg ttgaggtctc accccctagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgcccctga gacgcgtcaa tcgagttcgt acctaagggc gacaccccct aattagcccg ggcgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat ggggagtccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaacaagg ggtgttatga gccatattca ggtataaatg ggctcgcgat aatgttcaga attggttaat tggttgtaac actgacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag aatatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgccactt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcgttcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cactcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg cctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taagcggcgc gccatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatatgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaggac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagata gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgccagtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtga
Details for AsfuA.00629.a.HA11.GE00474
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 72kDa |
| OBSERVED MW: | 72kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGKDLD EYKKLYDESI RSPDTFWARM ARELLTFDKD FQTTHIGSLE NGDNAWFVEG RLNASFNCVD RHAIKNPNKV AIIYEADEPN EGRIITYGEL LREVSRVAWV LKQRGVKKGD TVAIYLPMIP EAVVAFLACA RIGAIHSVVF AGFSSDSLRD RVLDAGSKVV ITTDEGKRGG KVIGTKRIVD EALKQCPDVT SVLVYKRTGA EVPWTNGRDI WWHEEVEKYP CYVAPESMSS EDPLFLLYTS GSTGKPKGVM HTTAGYLLGA AMTGKYVFDI HDDDRFFCGG DVGWITGHTY VVYAPLLLGC STVVFESTPA YPNFSRYWDV IEKHKVTQFY VAPTALRLLK RAGDEHIHHK MEHLRILGSV GEPIAAEVWK WYFEKVGKEE AHICDTYWQT ETGSHVISPL GGITSTKPGS ASLPFFGIEP AIIDPVSGEE ISGNDVEGVL AFKQPWPSMA RTVWGAHKRY MDTYLNVYKG YYFTGDGAGR DHDGYYWIRG RVDDVVNVSG HRLSTAEIEA ALIEHHQVAE AAVVGIADEL TGQAVNAFVS LKDGNETTEQ VRKDLVMQVR KSIGPFAAPK AVFVVEDLPK TRSGKIMRRI LRKILSGEED SLGDTSTLSD PSVVNKIIET VHA
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgcaccatc accaccacca tcatcacgag aatctgtact tccaaggcaa agacctggac gagtacaaaa agttatatga tgagtccatc cgttcgccgg ataccttttg ggcacgtatg gctcgcgagc tgctgacgtt tgataaagac ttccaaacca cccacatcgg tagcttggag aacggtgata atgcatggtt cgttgagggt cgtctgaacg ccagctttaa ctgtgtcgac cgtcatgcaa tcaagaaccc gaacaaagtc gcgatcattt acgaggctga cgagccgaac gagggccgca tcatcactta tggtgagctg ctgcgtgagg ttagccgcgt ggcgtgggtc ctgaaacagc gtggtgtcaa aaagggcgat acggttgcga tttacttgcc gatgattccg gaagccgttg tggcgttcct ggcgtgtgca cgcatcggtg cgattcactc tgtggttttc gcgggtttta gcagcgatag cctgcgcgac cgcgtgctgg atgctggtag caaagttgtt atcacgaccg acgagggcaa gcgcggtggc aaggttatcg gtaccaagcg tattgttgac gaagcgttga aacagtgccc tgacgtcacg agcgtgctgg tgtacaagcg tacgggcgca gaagtgccgt ggacgaatgg tcgcgatatt tggtggcacg aagaggtcga gaagtatcca tgctacgtgg caccagagtc catgtcctcc gaggacccgc tgtttctgct gtacacctcc ggtagcactg gtaagccgaa gggtgttatg cacaccaccg cgggttattt gctgggtgcg gcgatgaccg gtaaatacgt ctttgacatt cacgacgacg atcgcttctt ctgcggtggc gatgtcggct ggatcacggg ccacacctac gtcgtctatg ccccgctgct gctgggttgc agcaccgtgg tctttgaaag caccccggca tacccgaatt tcagccgtta ctgggacgtg attgaaaagc acaaagtcac gcagttctat gtggcgccga cggcgctccg tctgctgaag cgtgcgggcg acgagcacat ccatcacaaa atggagcact tgcgtatcct gggtagcgtt ggcgagccta ttgctgcaga ggtgtggaaa tggtattttg aaaaagtcgg caaagaagag gcacacatct gtgatactta ctggcagacg gaaaccggtt cgcatgtaat tagcccgctg ggtggcatca ccagcactaa accgggcagc gcgagcctgc ctttcttcgg tattgagccg gctatcattg atccggtgag cggcgaagaa atcagcggta acgacgtcga gggcgtcctg gcctttaaac agccgtggcc gagcatggcg cgcacggtgt ggggtgcgca taagcgttac atggatacct atctgaatgt atacaagggc tactatttca ccggtgacgg tgcgggtcgt gatcatgacg gttactattg gattcgtggc cgtgtggatg atgttgttaa tgtgagcggt caccgcctta gcacggccga aattgaagct gcgctgattg agcatcatca agtggccgag gcagccgttg tcggcatcgc cgacgaactg accggccaag ccgttaatgc gtttgtgtcg ctgaaggacg gtaacgaaac gaccgaacaa gttcgtaaag acctggtcat gcaagtccgt aagtctatcg gcccgttcgc ggcaccgaaa gctgtttttg ttgtggaaga tttgccgaaa acccgtagcg gtaagattat gcgtcgcatt ctgcgtaaga ttctgtccgg tgaagaggac agcctgggtg atacgagcac cctgtctgat ccgagcgtgg tgaacaagat catcgaaacc gttcacgcgt gataagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for AsfuA.00629.a.FS11.PD00398
| PURIFICATION DATe: | 9/27/2015 |
| CONCENTRATION: | 19.9mg/ml |
| OBSERVED MW: | 76kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol |
| EXPRESSION HOST: | Data not available |
| VIAL COUNT (approx.): | 22 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGSDGD VKPAKPAVVL EANDVDTFHV PKAFYEKHPS KTHLKDLDEY KKLYDESIRS PDTFWARMAR ELLTFDKDFQ TTHIGSLENG DNAWFVEGRL NASFNCVDRH AIKNPNKVAI IYEADEPNEG RIITYGELLR EVSRVAWVLK QRGVKKGDTV AIYLPMIPEA VVAFLACARI GAIHSVVFAG FSSDSLRDRV LDAGSKVVIT TDEGKRGGKV IGTKRIVDEA LKQCPDVTSV LVYKRTGAEV PWTNGRDIWW HEEVEKYPCY VAPESMSSED PLFLLYTSGS TGKPKGVMHT TAGYLLGAAM TGKYVFDIHD DDRFFCGGDV GWITGHTYVV YAPLLLGCST VVFESTPAYP NFSRYWDVIE KHKVTQFYVA PTALRLLKRA GDEHIHHKME HLRILGSVGE PIAAEVWKWY FEKVGKEEAH ICDTYWQTET GSHVISPLGG ITSTKPGSAS LPFFGIEPAI IDPVSGEEIS GNDVEGVLAF KQPWPSMART VWGAHKRYMD TYLNVYKGYY FTGDGAGRDH DGYYWIRGRV DDVVNVSGHR LSTAEIEAAL IEHHQVAEAA VVGIADELTG QAVNAFVSLK DGNETTEQVR KDLVMQVRKS IGPFAAPKAV FVVEDLPKTR SGKIMRRILR KILSGEEDSL GDTSTLSDPS VVNKIIETVH AARAK
Full NT Sequence (Expression Vector + Insert)
TTAATAAGAT GATCTTCTTG AGATCGTTTT GGTCTGCGCG TAATCTCTTG CTCTGAAAAC GAAAAAACCG CCTTGCAGGG CGGTTTTTCG AAGGTTCTCT GAGCTACCAA CTCTTTGAAC CGAGGTAACT GGCTTGGAGG AGCGCAGTCA CCAAAACTTG TCCTTTCAGT TTAGCCTTAA CCGGCGCATG ACTTCAAGAC TAACTCCTCT AAATCAATTA CCAGTGGCTG CTGCCAGTGG TGCTTTTGCA TGTCTTTCCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGACTGA ACGGGGGGTT CGTGCATACA GTCCAGCTTG GAGCGAACTG CCTACCCGGA ACTGAGTGTC AGGCGTGGAA TGAGACAAAC GCGGCCATAA CAGCGGAATG ACACCGGTAA ACCGAAAGGC AGGAACAGGA GAGCGCACGA GGGAGCCGCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC ATAGTAAGCC AGTATACACT CCGCTAGCGC TGAGGTCCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGGCG AGAGTAGGGA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTCT GTGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCCCT GGGCGGTTCT GATAACGAGT AATCGTTAAT CCGCAAATAA CGTAAAAACC CGCTTCGGCG GGTTTTTTTA TGGGGGGAGT TTAGGGAAAG AGCATTTGTC AGAATATTTA AGGGCGCCTG TCACTTTGCT TGATATATGA GAATTATTTA ACCTTATAAA TGAGAAAAAA GCAACGCACT TTAAATAAGA TACGTTGCTT TTTCGATTGA TGAACACCTA TAATTAAACT ATTCATCTAT TATTTATGAT TTTTTGTATA TACAATATTT CTAGTTTGTT AAAGAGAATT AAGAAAATAA ATCTCGAAAA TAATAAAGGG AAAATCAGTT TTTGATATCA AAATTATACA TGTCAACGAT AATACAAAAT ATAATACAAA CTATAAGATG TTATCAGTAT TTATTATGCA TTTAGAATAA ATTTTGTGTC GCCCTTCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC CTCTAGAAAT AATTTTGTTT AACTTTTAGA GACCAAGGAG GTAAAAAATG CACCATCACC ATCATCACCA CCATGAAAAT CTGTATTTCC AGGGTAGCGA CGGCGACGTG AAGCCAGCCA AACCGGCAGT TGTGCTGGAA GCGAATGACG TCGATACCTT CCACGTTCCG AAAGCATTTT ACGAGAAACA TCCGAGCAAG ACGCACCTGA AAGACCTGGA CGAGTACAAA AAGTTATATG ATGAGTCCAT CCGTTCGCCG GATACCTTTT GGGCACGTAT GGCTCGCGAG CTGCTGACGT TTGATAAAGA CTTCCAAACC ACCCACATCG GTAGCTTGGA GAACGGTGAT AATGCATGGT TCGTTGAGGG TCGTCTGAAC GCCAGCTTTA ACTGTGTCGA CCGTCATGCA ATCAAGAACC CGAACAAAGT CGCGATCATT TACGAGGCTG ACGAGCCGAA CGAGGGCCGC ATCATCACTT ATGGTGAGCT GCTGCGTGAG GTTAGCCGCG TGGCGTGGGT CCTGAAACAG CGTGGTGTCA AAAAGGGCGA TACGGTTGCG ATTTACTTGC CGATGATTCC GGAAGCCGTT GTGGCGTTCC TGGCGTGTGC ACGCATCGGT GCGATTCACT CTGTGGTTTT CGCGGGTTTT AGCAGCGATA GCCTGCGCGA CCGCGTGCTG GATGCTGGTA GCAAAGTTGT TATCACGACC GACGAGGGCA AGCGCGGTGG CAAGGTTATC GGTACCAAGC GTATTGTTGA CGAAGCGTTG AAACAGTGCC CTGACGTCAC GAGCGTGCTG GTGTACAAGC GTACGGGCGC AGAAGTGCCG TGGACGAATG GTCGCGATAT TTGGTGGCAC GAAGAGGTCG AGAAGTATCC ATGCTACGTG GCACCAGAGT CCATGTCCTC CGAGGACCCG CTGTTTCTGC TGTACACCTC CGGTAGCACT GGTAAGCCGA AGGGTGTTAT GCACACCACC GCGGGTTATT TGCTGGGTGC GGCGATGACC GGTAAATACG TCTTTGACAT TCACGACGAC GATCGCTTCT TCTGCGGTGG CGATGTCGGC TGGATCACGG GCCACACCTA CGTCGTCTAT GCCCCGCTGC TGCTGGGTTG CAGCACCGTG GTCTTTGAAA GCACCCCGGC ATACCCGAAT TTCAGCCGTT ACTGGGACGT GATTGAAAAG CACAAAGTCA CGCAGTTCTA TGTGGCGCCG ACGGCGCTCC GTCTGCTGAA GCGTGCGGGC GACGAGCACA TCCATCACAA AATGGAGCAC TTGCGTATCC TGGGTAGCGT TGGCGAGCCT ATTGCTGCAG AGGTGTGGAA ATGGTATTTT GAAAAAGTCG GCAAAGAAGA GGCACACATC TGTGATACTT ACTGGCAGAC GGAAACCGGT TCGCATGTAA TTAGCCCGCT GGGTGGCATC ACCAGCACTA AACCGGGCAG CGCGAGCCTG CCTTTCTTCG GTATTGAGCC GGCTATCATT GATCCGGTGA GCGGCGAAGA AATCAGCGGT AACGACGTCG AGGGCGTCCT GGCCTTTAAA CAGCCGTGGC CGAGCATGGC GCGCACGGTG TGGGGTGCGC ATAAGCGTTA CATGGATACC TATCTGAATG TATACAAGGG CTACTATTTC ACCGGTGACG GTGCGGGTCG TGATCATGAC GGTTACTATT GGATTCGTGG CCGTGTGGAT GATGTTGTTA ATGTGAGCGG TCACCGCCTT AGCACGGCCG AAATTGAAGC TGCGCTGATT GAGCATCATC AAGTGGCCGA GGCAGCCGTT GTCGGCATCG CCGACGAACT GACCGGCCAA GCCGTTAATG CGTTTGTGTC GCTGAAGGAC GGTAACGAAA CGACCGAACA AGTTCGTAAA GACCTGGTCA TGCAAGTCCG TAAGTCTATC GGCCCGTTCG CGGCACCGAA AGCTGTTTTT GTTGTGGAAG ATTTGCCGAA AACCCGTAGC GGTAAGATTA TGCGTCGCAT TCTGCGTAAG ATTCTGTCCG GTGAAGAGGA CAGCCTGGGT GATACGAGCA CCCTGTCTGA TCCGAGCGTG GTGAACAAGA TCATCGAAAC CGTTCACGCG GCACGTGCCA AGTAATGAGG TTGAGGTCTC ACCCCCTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCCCCTGA GACGCGTCAA TCGAGTTCGT ACCTAAGGGC GACACCCCCT AATTAGCCCG GGCGAAAGGC CCAGTCTTTC GACTGAGCCT TTCGTTTTAT TTGATGCCTG GCAGTTCCCT ACTCTCGCAT GGGGAGTCCC CACACTACCA TCGGCGCTAC GGCGTTTCAC TTCTGAGTTC GGCATGGGGT CAGGTGGGAC CACCGCGCTA CTGCCGCCAG GCAAACAAGG GGTGTTATGA GCCATATTCA GGTATAAATG GGCTCGCGAT AATGTTCAGA ATTGGTTAAT TGGTTGTAAC ACTGACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AATATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCACTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCGTTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CACTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG CCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAAGCGGCGC GCCATCGAAT GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATATGAA ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAGGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGATATCT CGGTAGTGGG ATACGACGAT ACCGAAGATA GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCAGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGA
Details for AsfuA.00629.a.HA11.PD00404
| PURIFICATION DATe: | 10/4/2015 |
| CONCENTRATION: | 20.22mg/ml |
| OBSERVED MW: | 72kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 10mM Tris pH 8.5, 20mM NaCl |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 24 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGKDLD EYKKLYDESI RSPDTFWARM ARELLTFDKD FQTTHIGSLE NGDNAWFVEG RLNASFNCVD RHAIKNPNKV AIIYEADEPN EGRIITYGEL LREVSRVAWV LKQRGVKKGD TVAIYLPMIP EAVVAFLACA RIGAIHSVVF AGFSSDSLRD RVLDAGSKVV ITTDEGKRGG KVIGTKRIVD EALKQCPDVT SVLVYKRTGA EVPWTNGRDI WWHEEVEKYP CYVAPESMSS EDPLFLLYTS GSTGKPKGVM HTTAGYLLGA AMTGKYVFDI HDDDRFFCGG DVGWITGHTY VVYAPLLLGC STVVFESTPA YPNFSRYWDV IEKHKVTQFY VAPTALRLLK RAGDEHIHHK MEHLRILGSV GEPIAAEVWK WYFEKVGKEE AHICDTYWQT ETGSHVISPL GGITSTKPGS ASLPFFGIEP AIIDPVSGEE ISGNDVEGVL AFKQPWPSMA RTVWGAHKRY MDTYLNVYKG YYFTGDGAGR DHDGYYWIRG RVDDVVNVSG HRLSTAEIEA ALIEHHQVAE AAVVGIADEL TGQAVNAFVS LKDGNETTEQ VRKDLVMQVR KSIGPFAAPK AVFVVEDLPK TRSGKIMRRI LRKILSGEED SLGDTSTLSD PSVVNKIIET VHA
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGCACCATC ACCACCACCA TCATCACGAG AATCTGTACT TCCAAGGCAA AGACCTGGAC GAGTACAAAA AGTTATATGA TGAGTCCATC CGTTCGCCGG ATACCTTTTG GGCACGTATG GCTCGCGAGC TGCTGACGTT TGATAAAGAC TTCCAAACCA CCCACATCGG TAGCTTGGAG AACGGTGATA ATGCATGGTT CGTTGAGGGT CGTCTGAACG CCAGCTTTAA CTGTGTCGAC CGTCATGCAA TCAAGAACCC GAACAAAGTC GCGATCATTT ACGAGGCTGA CGAGCCGAAC GAGGGCCGCA TCATCACTTA TGGTGAGCTG CTGCGTGAGG TTAGCCGCGT GGCGTGGGTC CTGAAACAGC GTGGTGTCAA AAAGGGCGAT ACGGTTGCGA TTTACTTGCC GATGATTCCG GAAGCCGTTG TGGCGTTCCT GGCGTGTGCA CGCATCGGTG CGATTCACTC TGTGGTTTTC GCGGGTTTTA GCAGCGATAG CCTGCGCGAC CGCGTGCTGG ATGCTGGTAG CAAAGTTGTT ATCACGACCG ACGAGGGCAA GCGCGGTGGC AAGGTTATCG GTACCAAGCG TATTGTTGAC GAAGCGTTGA AACAGTGCCC TGACGTCACG AGCGTGCTGG TGTACAAGCG TACGGGCGCA GAAGTGCCGT GGACGAATGG TCGCGATATT TGGTGGCACG AAGAGGTCGA GAAGTATCCA TGCTACGTGG CACCAGAGTC CATGTCCTCC GAGGACCCGC TGTTTCTGCT GTACACCTCC GGTAGCACTG GTAAGCCGAA GGGTGTTATG CACACCACCG CGGGTTATTT GCTGGGTGCG GCGATGACCG GTAAATACGT CTTTGACATT CACGACGACG ATCGCTTCTT CTGCGGTGGC GATGTCGGCT GGATCACGGG CCACACCTAC GTCGTCTATG CCCCGCTGCT GCTGGGTTGC AGCACCGTGG TCTTTGAAAG CACCCCGGCA TACCCGAATT TCAGCCGTTA CTGGGACGTG ATTGAAAAGC ACAAAGTCAC GCAGTTCTAT GTGGCGCCGA CGGCGCTCCG TCTGCTGAAG CGTGCGGGCG ACGAGCACAT CCATCACAAA ATGGAGCACT TGCGTATCCT GGGTAGCGTT GGCGAGCCTA TTGCTGCAGA GGTGTGGAAA TGGTATTTTG AAAAAGTCGG CAAAGAAGAG GCACACATCT GTGATACTTA CTGGCAGACG GAAACCGGTT CGCATGTAAT TAGCCCGCTG GGTGGCATCA CCAGCACTAA ACCGGGCAGC GCGAGCCTGC CTTTCTTCGG TATTGAGCCG GCTATCATTG ATCCGGTGAG CGGCGAAGAA ATCAGCGGTA ACGACGTCGA GGGCGTCCTG GCCTTTAAAC AGCCGTGGCC GAGCATGGCG CGCACGGTGT GGGGTGCGCA TAAGCGTTAC ATGGATACCT ATCTGAATGT ATACAAGGGC TACTATTTCA CCGGTGACGG TGCGGGTCGT GATCATGACG GTTACTATTG GATTCGTGGC CGTGTGGATG ATGTTGTTAA TGTGAGCGGT CACCGCCTTA GCACGGCCGA AATTGAAGCT GCGCTGATTG AGCATCATCA AGTGGCCGAG GCAGCCGTTG TCGGCATCGC CGACGAACTG ACCGGCCAAG CCGTTAATGC GTTTGTGTCG CTGAAGGACG GTAACGAAAC GACCGAACAA GTTCGTAAAG ACCTGGTCAT GCAAGTCCGT AAGTCTATCG GCCCGTTCGC GGCACCGAAA GCTGTTTTTG TTGTGGAAGA TTTGCCGAAA ACCCGTAGCG GTAAGATTAT GCGTCGCATT CTGCGTAAGA TTCTGTCCGG TGAAGAGGAC AGCCTGGGTG ATACGAGCAC CCTGTCTGAT CCGAGCGTGG TGAACAAGAT CATCGAAACC GTTCACGCGT GATAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T
Details for AsfuA.00629.a.FS11.PD00415
| PURIFICATION DATe: | 1/4/2016 |
| CONCENTRATION: | 10.1mg/ml |
| OBSERVED MW: | 76kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 10 mM Tris pH 8.5, 20 mM NaCl |
| EXPRESSION HOST: | Data not available |
| VIAL COUNT (approx.): | 52 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGSDGD VKPAKPAVVL EANDVDTFHV PKAFYEKHPS KTHLKDLDEY KKLYDESIRS PDTFWARMAR ELLTFDKDFQ TTHIGSLENG DNAWFVEGRL NASFNCVDRH AIKNPNKVAI IYEADEPNEG RIITYGELLR EVSRVAWVLK QRGVKKGDTV AIYLPMIPEA VVAFLACARI GAIHSVVFAG FSSDSLRDRV LDAGSKVVIT TDEGKRGGKV IGTKRIVDEA LKQCPDVTSV LVYKRTGAEV PWTNGRDIWW HEEVEKYPCY VAPESMSSED PLFLLYTSGS TGKPKGVMHT TAGYLLGAAM TGKYVFDIHD DDRFFCGGDV GWITGHTYVV YAPLLLGCST VVFESTPAYP NFSRYWDVIE KHKVTQFYVA PTALRLLKRA GDEHIHHKME HLRILGSVGE PIAAEVWKWY FEKVGKEEAH ICDTYWQTET GSHVISPLGG ITSTKPGSAS LPFFGIEPAI IDPVSGEEIS GNDVEGVLAF KQPWPSMART VWGAHKRYMD TYLNVYKGYY FTGDGAGRDH DGYYWIRGRV DDVVNVSGHR LSTAEIEAAL IEHHQVAEAA VVGIADELTG QAVNAFVSLK DGNETTEQVR KDLVMQVRKS IGPFAAPKAV FVVEDLPKTR SGKIMRRILR KILSGEEDSL GDTSTLSDPS VVNKIIETVH AARAK
Full NT Sequence (Expression Vector + Insert)
TTAATAAGAT GATCTTCTTG AGATCGTTTT GGTCTGCGCG TAATCTCTTG CTCTGAAAAC GAAAAAACCG CCTTGCAGGG CGGTTTTTCG AAGGTTCTCT GAGCTACCAA CTCTTTGAAC CGAGGTAACT GGCTTGGAGG AGCGCAGTCA CCAAAACTTG TCCTTTCAGT TTAGCCTTAA CCGGCGCATG ACTTCAAGAC TAACTCCTCT AAATCAATTA CCAGTGGCTG CTGCCAGTGG TGCTTTTGCA TGTCTTTCCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGACTGA ACGGGGGGTT CGTGCATACA GTCCAGCTTG GAGCGAACTG CCTACCCGGA ACTGAGTGTC AGGCGTGGAA TGAGACAAAC GCGGCCATAA CAGCGGAATG ACACCGGTAA ACCGAAAGGC AGGAACAGGA GAGCGCACGA GGGAGCCGCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC ATAGTAAGCC AGTATACACT CCGCTAGCGC TGAGGTCCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGGCG AGAGTAGGGA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTCT GTGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCCCT GGGCGGTTCT GATAACGAGT AATCGTTAAT CCGCAAATAA CGTAAAAACC CGCTTCGGCG GGTTTTTTTA TGGGGGGAGT TTAGGGAAAG AGCATTTGTC AGAATATTTA AGGGCGCCTG TCACTTTGCT TGATATATGA GAATTATTTA ACCTTATAAA TGAGAAAAAA GCAACGCACT TTAAATAAGA TACGTTGCTT TTTCGATTGA TGAACACCTA TAATTAAACT ATTCATCTAT TATTTATGAT TTTTTGTATA TACAATATTT CTAGTTTGTT AAAGAGAATT AAGAAAATAA ATCTCGAAAA TAATAAAGGG AAAATCAGTT TTTGATATCA AAATTATACA TGTCAACGAT AATACAAAAT ATAATACAAA CTATAAGATG TTATCAGTAT TTATTATGCA TTTAGAATAA ATTTTGTGTC GCCCTTCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC CTCTAGAAAT AATTTTGTTT AACTTTTAGA GACCAAGGAG GTAAAAAATG CACCATCACC ATCATCACCA CCATGAAAAT CTGTATTTCC AGGGTAGCGA CGGCGACGTG AAGCCAGCCA AACCGGCAGT TGTGCTGGAA GCGAATGACG TCGATACCTT CCACGTTCCG AAAGCATTTT ACGAGAAACA TCCGAGCAAG ACGCACCTGA AAGACCTGGA CGAGTACAAA AAGTTATATG ATGAGTCCAT CCGTTCGCCG GATACCTTTT GGGCACGTAT GGCTCGCGAG CTGCTGACGT TTGATAAAGA CTTCCAAACC ACCCACATCG GTAGCTTGGA GAACGGTGAT AATGCATGGT TCGTTGAGGG TCGTCTGAAC GCCAGCTTTA ACTGTGTCGA CCGTCATGCA ATCAAGAACC CGAACAAAGT CGCGATCATT TACGAGGCTG ACGAGCCGAA CGAGGGCCGC ATCATCACTT ATGGTGAGCT GCTGCGTGAG GTTAGCCGCG TGGCGTGGGT CCTGAAACAG CGTGGTGTCA AAAAGGGCGA TACGGTTGCG ATTTACTTGC CGATGATTCC GGAAGCCGTT GTGGCGTTCC TGGCGTGTGC ACGCATCGGT GCGATTCACT CTGTGGTTTT CGCGGGTTTT AGCAGCGATA GCCTGCGCGA CCGCGTGCTG GATGCTGGTA GCAAAGTTGT TATCACGACC GACGAGGGCA AGCGCGGTGG CAAGGTTATC GGTACCAAGC GTATTGTTGA CGAAGCGTTG AAACAGTGCC CTGACGTCAC GAGCGTGCTG GTGTACAAGC GTACGGGCGC AGAAGTGCCG TGGACGAATG GTCGCGATAT TTGGTGGCAC GAAGAGGTCG AGAAGTATCC ATGCTACGTG GCACCAGAGT CCATGTCCTC CGAGGACCCG CTGTTTCTGC TGTACACCTC CGGTAGCACT GGTAAGCCGA AGGGTGTTAT GCACACCACC GCGGGTTATT TGCTGGGTGC GGCGATGACC GGTAAATACG TCTTTGACAT TCACGACGAC GATCGCTTCT TCTGCGGTGG CGATGTCGGC TGGATCACGG GCCACACCTA CGTCGTCTAT GCCCCGCTGC TGCTGGGTTG CAGCACCGTG GTCTTTGAAA GCACCCCGGC ATACCCGAAT TTCAGCCGTT ACTGGGACGT GATTGAAAAG CACAAAGTCA CGCAGTTCTA TGTGGCGCCG ACGGCGCTCC GTCTGCTGAA GCGTGCGGGC GACGAGCACA TCCATCACAA AATGGAGCAC TTGCGTATCC TGGGTAGCGT TGGCGAGCCT ATTGCTGCAG AGGTGTGGAA ATGGTATTTT GAAAAAGTCG GCAAAGAAGA GGCACACATC TGTGATACTT ACTGGCAGAC GGAAACCGGT TCGCATGTAA TTAGCCCGCT GGGTGGCATC ACCAGCACTA AACCGGGCAG CGCGAGCCTG CCTTTCTTCG GTATTGAGCC GGCTATCATT GATCCGGTGA GCGGCGAAGA AATCAGCGGT AACGACGTCG AGGGCGTCCT GGCCTTTAAA CAGCCGTGGC CGAGCATGGC GCGCACGGTG TGGGGTGCGC ATAAGCGTTA CATGGATACC TATCTGAATG TATACAAGGG CTACTATTTC ACCGGTGACG GTGCGGGTCG TGATCATGAC GGTTACTATT GGATTCGTGG CCGTGTGGAT GATGTTGTTA ATGTGAGCGG TCACCGCCTT AGCACGGCCG AAATTGAAGC TGCGCTGATT GAGCATCATC AAGTGGCCGA GGCAGCCGTT GTCGGCATCG CCGACGAACT GACCGGCCAA GCCGTTAATG CGTTTGTGTC GCTGAAGGAC GGTAACGAAA CGACCGAACA AGTTCGTAAA GACCTGGTCA TGCAAGTCCG TAAGTCTATC GGCCCGTTCG CGGCACCGAA AGCTGTTTTT GTTGTGGAAG ATTTGCCGAA AACCCGTAGC GGTAAGATTA TGCGTCGCAT TCTGCGTAAG ATTCTGTCCG GTGAAGAGGA CAGCCTGGGT GATACGAGCA CCCTGTCTGA TCCGAGCGTG GTGAACAAGA TCATCGAAAC CGTTCACGCG GCACGTGCCA AGTAATGAGG TTGAGGTCTC ACCCCCTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCCCCTGA GACGCGTCAA TCGAGTTCGT ACCTAAGGGC GACACCCCCT AATTAGCCCG GGCGAAAGGC CCAGTCTTTC GACTGAGCCT TTCGTTTTAT TTGATGCCTG GCAGTTCCCT ACTCTCGCAT GGGGAGTCCC CACACTACCA TCGGCGCTAC GGCGTTTCAC TTCTGAGTTC GGCATGGGGT CAGGTGGGAC CACCGCGCTA CTGCCGCCAG GCAAACAAGG GGTGTTATGA GCCATATTCA GGTATAAATG GGCTCGCGAT AATGTTCAGA ATTGGTTAAT TGGTTGTAAC ACTGACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AATATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCACTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCGTTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CACTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG CCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAAGCGGCGC GCCATCGAAT GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATATGAA ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAGGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGATATCT CGGTAGTGGG ATACGACGAT ACCGAAGATA GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCAGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGA