SSGCID: Target Page

AsfuA.00174.a

Serine/threonine-protein phosphatase 2B catalytic subunit (EC 3.1.3.16) (Calmodulin-dependent calcineurin A subunit)

CENTER ID:	AsfuA.00174.a
ORGANISM:	Aspergillus fumigatus
ASSOCIATED DISEASE:	Aspergillosis
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AsfuA.00174.a.FW11.GE00468	full length		2	435

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:Afu5g09360
RefSeq:	XP_753703.1
UniProt:	Q4WUR1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEDGTQVSTL ERVIKEVQAP ALSTPTDEMF WSPEDPSKPN LQFLKQHFYR EGRLTEEQAL WIIHAGTQIL RSEPNLLEMD APITVCGDVH GQYYDLMKLF EVGGDPSETR YLFLGDYVDR GYFSIECVLY LWALKIWYPN SLWLLRGNHE CRHLTDYFTF KLECKHKYSE RIYEACIESF CALPLAAVMN KQFLCIHGGL SPELHTLEDI KSIDRFREPP THGLMCDILW ADPLEEFGQE KTGDYFVHNS VRGCSYFFSY PAACAFLEKN NLLSIIRAHE AQDAGYRMYR KTRTTGFPSV MTIFSAPNYL DVYNNKAAVL KYENNVMNIR QFNCTPHPYW LPNFMDVFTW SLPFVGEKIT DMLIAILNTC SKEELEDETP TSVSPSAPSP PLPMDVESSE FKRRAIKNKI LAIGRLSRVF QVLREESERV TELKTAAGGR LPAGTLMLGA EGIKQAITNF EDARKVDLQN ERLPPSHEEV IKRSEEERRA ALERAQQEAD NDTGLATVAR RISMSAGSGR SRRQRDAARE TREA

NT Sequence

atggatcaag cactggcgcg cgcggtgagg gacaagaagc ccgtcccgga gatcgacttc accctacaca ccatggagga tggcactcaa gtctccacgc tggaacgagt gatcaaagag gtgcaagcgc cggctttaag tactcccacg gatgaaatgt tctggtctcc tgaagacccc tcgaagccca acctccagtt cctgaaacag catttctatc gggaaggtcg tcttacagag gagcaggcgc tatggattat acatgccgga actcagattc tgagatctga acctaatctg ctagagatgg atgcgccgat caccgtctgt ggcgacgttc acggtcaata ctatgacttg atgaaactct tcgaagtcgg tggcgatccg tccgagacga ggtatctttt cctgggtgat tatgtcgaca gaggttattt cagcattgag tgcgttttat atctctgggc cctgaagatc tggtacccga acagcctttg gctgctccga ggcaaccacg aatgcaggca tctcacggat tacttcacct tcaagctgga gtgcaagcat aaatacagcg agcgtatcta tgaggcctgc attgaatcat tctgcgcgtt gccgctggcg gcggtcatga acaagcaatt cctgtgtata catggtggcc taagtcctga acttcacact ctcgaagata tcaaatcgat tgatcgtttc agagagcccc cgactcacgg tttgatgtgc gacatcctgt gggctgatcc gcttgaagag ttcggacaag aaaaaacggg agattacttt gttcacaaca gtgttcgagg ctgttcttac ttcttctcgt accccgcggc ttgcgctttc cttgaaaaga acaacctatt gtcgatcatt cgagcacacg aggcgcagga cgctggatat cgcatgtacc gaaaaactcg cacgaccgga tttccaagtg tgatgactat cttcagtgct cccaactact tggatgtcta caacaacaag gctgccgtgc tgaagtacga gaacaacgtc atgaatatcc gacagttcaa ctgcacgcct cacccgtact ggttgccaaa cttcatggac gtgtttacgt ggtcgcttcc tttcgtcggt gagaagatca ccgatatgct catcgccatt ctcaatacct gctccaagga ggagctcgaa gacgagactc ctacctccgt gtcaccttct gctccgtctc ctccgctccc aatggacgtg gagagcagcg aattcaaaag acgggcgatc aagaacaaga ttcttgccat cggacgtctc tcgcgtgtct tccaggtgtt gcgggaggag tctgagagag ttacggaact caagacagct gctggtggcc gtctcccagc tggtaccctg atgcttggcg ccgaaggaat caaacaggct attaccaact ttgaggacgc tcgcaaggtt gacttgcaga acgagcgtct tccaccgtct cacgaggagg tcatcaagag aagcgaagag gaaagacggg ctgccctcga gcgtgcgcaa caggaggcag acaatgatac cggcttggct accgtcgccc ggagaattag catgtccgcc ggctctggca ggtctcgtcg gcaaagagat gccgcccggg agactaggga agcctaa

Details for AsfuA.00174.a.FW11.GE00468

HARVESTED ON:	8/10/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Data Unavailable
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGSSHHHHHH SSGENLYFQG SEDGTQVSTL ERVIKEVQAP ALSTPTDEMF WSPEDPSKPN LQFLKQHFYR EGRLTEEQAL WIIHAGTQIL RSEPNLLEMD APITVCGDVH GQYYDLMKLF EVGGDPSETR YLFLGDYVDR GYFSIECVLY LWALKIWYPN SLWLLRGNHE CRHLTDYFTF KLECKHKYSE RIYEACIESF CALPLAAVMN KQFLCIHGGL SPELHTLEDI KSIDRFREPP THGLMCDILW ADPLEEFGQE KTGDYFVHNS VRGCSYFFSY PAACAFLEKN NLLSIIRAHE AQDAGYRMYR KTRTTGFPSV MTIFSAPNYL DVYNNKAAVL KYENNVMNIR QFNCTPHPYW LPNFMDVFTW SLPFVGEKIT DMLIAILNTC SKEELEDETP TSVSPSAPSP PLPMDVESSE FKRRAIKNKI LAIGRLSRVF QVLREESERV TELK

Full NT Sequence (Expression Vector + Insert)

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg cggatctatg ggcagcagcc atcaccatca tcaccactct tccggcgaga acctgtattt ccagggatcc gaggatggta cccaggtttc cactctggag cgtgtgatca aagaagtgca ggcaccagca ctgagcactc cgactgacga aatgttctgg tctccggaag atccgtctaa accaaacctg cagttcctga aacaacactt ctatcgcgag ggccgtctga ctgaagaaca ggctctgtgg ataatccacg ctggtactca gatccttcgc tccgaaccaa acttactgga aatggacgca cctataaccg tgtgcggtga cgttcatggt cagtactatg acctgatgaa actgtttgag gtgggcggtg atccatctga gactcgttac ctgttcttag gcgattacgt tgaccgtggt tactttagca tcgagtgtgt cctgtacctg tgggccctga aaatctggta tccgaactcc ctctggctcc tgcgtggcaa tcatgaatgc cgccacctga ccgactattt cacctttaaa ctggaatgca agcacaaata cagcgaacgc atctacgaag catgcattga atccttctgc gcactgccat tagctgctgt tatgaacaag cagttcctgt gcatccacgg cggtctgagc ccggaactcc acacgttgga agatatcaag tctattgacc gtttccgcga gcctccaacc cacggtctga tgtgtgatat tctgtgggct gacccgctgg aagaatttgg tcaggagaag accggcgatt acttcgtaca taactctgtt cgcggttgta gctatttctt ctcctacccg gcagcctgcg cattcttaga gaagaataac ctgctgagca ttattcgtgc tcatgaagct caagatgccg gctatcgcat gtaccgtaag acccgcacta ccggctttcc atccgtgatg actatcttta gcgctccaaa ttaccttgat gtttataaca ataaagctgc tgtactgaaa tatgagaata acgtgatgaa cattcgtcag ttcaattgta ctcctcaccc gtactggctg cctaacttca tggacgtgtt cacctggtct ctcccattcg ttggtgaaaa aatcaccgac atgctcatcg caatcctgaa tacttgctca aaggaggagt tggaggatga gacacccaca tcagtctcac ccagtgcccc cagtccaccc cttcctatgg atgtggagag ttcggagttc aagcggagag ccattaagaa taagattctt gccattggac ggcttagtag ggtctttcaa gtgcttcgag aggagtcaga gagagtgaca gagttgaagt gataagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctaggag atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgg