SSGCID: Target Page

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
AqaeA.00166.a.DG11.GE00612	full length	1	272
AqaeA.00166.a.DG12.GE00613	full length	1	275
AqaeA.00166.a.EV11.GE00586	full length	1	283
AqaeA.00166.a.EV12.GE41552	full length	1	283
AqaeA.00166.a.JA11.GE41553	full length	1	282
AqaeA.00166.a.JA12.GE41554	full length	1	282

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AqaeA.00166.a.DG12.PD00470	full length		1	275

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224324.8.peg.1368
RefSeq:	WP_010881151.1
UniProt:	O67648

Details for AqaeA.00166.a.DG11.GE00612

HARVESTED ON:	8/30/2016
SEQUENCED ON:	10/25/2017
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMGLE KTVKEKLSFE GVGIHTGEYS KLIIHPEKEG TGIRFFKNGV YIPARHEFVV HTNHSTDLGF KGQRIKTVEH ILSVLHLLEI TNVTIEVIGN EIPILDGSGW EFYEAIRKNI LNQNREIDYF VVEEPIIVED EGRLIKAEPS DTLEVTYEGE FKNFLGRQKF TFVEGNEEEI VLARTFAFDW EIEHIKKVGL GKGGSLKNTL VLGKDKVYNP EGLRYENEPV RHKVFDLIGD LYLLGSPVKG KFYSFRGGHS LNVKLVKELA KKQKLTR

Full NT Sequence (Expression Vector + Insert)

atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggaatg ggtttggaga aaactgtgaa agaaaaactg agcttcgagg gtgttggtat tcatacgggt gagtacagca agctgattat ccatccagag aaagagggca ccggcattcg tttcttcaaa aacggtgttt acatcccggc tcgtcatgaa tttgttgtcc acacgaatca cagcacggat ctgggcttta agggccagcg cattaagacc gtggagcaca ttctgtcggt gctgcatctg ctggaaatca ccaatgttac gattgaagtg atcggcaacg aaatcccgat tctggacggt agcggttggg aattttacga agcgatccgt aagaatatcc tgaatcaaaa ccgcgagatc gactattttg tggtcgagga accgatcatc gtggaagatg agggtcgtct gattaaagcc gagccgagcg acacgttgga agtcacctat gagggcgagt tcaagaactt tctgggccgt caaaagttca cctttgttga gggtaacgaa gaagaaattg ttctggcgcg cactttcgcg ttcgattggg aaattgagca catcaaaaaa gtcggcctgg gtaagggtgg ctccctgaag aacaccctgg tgctgggcaa agacaaagtc tataatcctg agggtctgcg ctatgagaat gaaccggttc gtcacaaagt ctttgacctc atcggtgacc tgtacttgct gggttccccg gtcaagggca agttctacag cttccgtggt ggccactctt tgaacgttaa actggtgaaa gaactggcga agaaacagaa actgacccgt tgataagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaacttga acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa ggagatggcg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atc

Details for AqaeA.00166.a.DG12.GE00613

HARVESTED ON:	8/26/2016
SEQUENCED ON:	10/25/2017
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMGLE KTVKEKLSFE GVGIHTGEYS KLIIHPEKEG TGIRFFKNGV YIPARHEFVV HTNHSTDLGF KGQRIKTVEH ILSVLHLLEI TNVTIEVIGN EIPILDGSGW EFYEAIRKNI LNQNREIDYF VVEEPIIVED EGRLIKAEPS DTLEVTYEGE FKNFLGRQKF TFVEGNEEEI VLARTFAFDW EIEHIKKVGL GKGGSLKNTL VLGKDKVYNP EGLRYENEPV RHKVFDLIGD LYLLGSPVKG KFYSFRGGHS LNVKLVKELA KKQ

Validated NT Sequence

tttctgtttc ttcgccagtt ctttcaccag tttaacgttc aaagagtggc caccacggaa gctgtagaac ttgcccttga ccggggaacc cagcaagtac aggtcaccga tgaggtcaaa gactttgtga cgaaccggtt cattctcata gcgcagaccc tcaggattat agactttgtc tttgcccagc accagggtgt tcttcaggga gccaccctta cccaggccga cttttttgat gtgctcaatt tcccaatcga acgcgaaagt gcgcgccaga acaatttctt cttcgttacc ctcaacaaag gtgaactttt gacggcccag aaagttcttg aactcgccct cataggtgac ttccaacgtg tcgctcggct cggctttaat cagacgaccc tcatcttcca cgatgatcgg ttcctcgacc acaaaatagt cgatctcgcg gttttgattc aggatattct tacggatcgc ttcgtaaaat tcccaaccgc taccgtccag aatcgggatt tcgttgccga tcacttcaat cgtaacattg gtgatttcca gcagatgcag caccgacaga atgtgctcca cggtcttaat gcgctggccc ttaaagccca gatccgtgct gtgattcgtg tggacaacaa attcatgacg agccgggatg taaacaccgt ttttgaagaa acgaatgccg gtgccctctt tctctggatg gataatcagc ttgctgtact cacccgtatg aataccaaca ccctcgaagc tcagtttttc tttcacagtt ttctccaaac ccattccacc gatttgttca cggtgtgctt cgataatgtc gttatcttcc atgtccagat cttccggagt ctggtctgcc tgaatacgga taccgtcgta cagaaagcgc agggaatcca tttccttgcc ctggcgttta gcgaatgctt ccatcaggcg acgcagcggg gtggtcttct taatcttgaa gaagatttcg ctgctaccgt cgctaacctt caggttgatg tgggtctccg gcttcacttc cggttttact tcgccgctat ggtggtggtg gtggtgggac at

Expected Protein Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMGLE KTVKEKLSFE GVGIHTGEYS KLIIHPEKEG TGIRFFKNGV YIPARHEFVV HTNHSTDLGF KGQRIKTVEH ILSVLHLLEI TNVTIEVIGN EIPILDGSGW EFYEAIRKNI LNQNREIDYF VVEEPIIVED EGRLIKAEPS DTLEVTYEGE FKNFLGRQKF TFVEGNEEEI VLARTFAFDW EIEHIKKVGL GKGGSLKNTL VLGKDKVYNP EGLRYENEPV RHKVFDLIGD LYLLGSPVKG KFYSFRGGHS LNVKLVKELA KKQKLTRDLP

Full NT Sequence (Expression Vector + Insert)

atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggaatg ggtttggaga aaactgtgaa agaaaaactg agcttcgagg gtgttggtat tcatacgggt gagtacagca agctgattat ccatccagag aaagagggca ccggcattcg tttcttcaaa aacggtgttt acatcccggc tcgtcatgaa tttgttgtcc acacgaatca cagcacggat ctgggcttta agggccagcg cattaagacc gtggagcaca ttctgtcggt gctgcatctg ctggaaatca ccaatgttac gattgaagtg atcggcaacg aaatcccgat tctggacggt agcggttggg aattttacga agcgatccgt aagaatatcc tgaatcaaaa ccgcgagatc gactattttg tggtcgagga accgatcatc gtggaagatg agggtcgtct gattaaagcc gagccgagcg acacgttgga agtcacctat gagggcgagt tcaagaactt tctgggccgt caaaagttca cctttgttga gggtaacgaa gaagaaattg ttctggcgcg cactttcgcg ttcgattggg aaattgagca catcaaaaaa gtcggcctgg gtaagggtgg ctccctgaag aacaccctgg tgctgggcaa agacaaagtc tataatcctg agggtctgcg ctatgagaat gaaccggttc gtcacaaagt ctttgacctc atcggtgacc tgtacttgct gggttccccg gtcaagggca agttctacag cttccgtggt ggccactctt tgaacgttaa actggtgaaa gaactggcga agaaacagaa actgacccgt gatctgccgt gataagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaacttgaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaag gagatggcgc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tc

Details for AqaeA.00166.a.EV11.GE00586

HARVESTED ON:	3/30/2016
SEQUENCED ON:	10/25/2017
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMGLE KTVKEKLSFE GVGIHTGEYS KLIIHPEKEG TGIRFFKNGV YIPARHEFVV HTNHSTDLGF KGQRIKTVEH ILSVLHLLEI TNVTIEVIGN EIPILDGSGW EFYEAIRKNI LNQNREIDYF VVEEPIIVED EGRLIKAEPS DTLEVTYEGE FKNFLGRQKF TFVEGNEEEI VLARTFCFDW EIEHIKKVGL GKGGSLKNTL VLGKDKVYNP EGLRYENEPV RHKVFDLIGD LYLLGSPVKG KFYSFRGGHS LNVKLVKELA KKQKLTR

Validated NT Sequence

atcacgggtc agtttctgtt tcttcgccag ttctttcacc agtttaacgt tcaaagagtg gccaccacgg aagctgtaga acttgccctt gaccggggaa cccagcaagt acaggtcacc gatgaggtca aagactttgt gacgaaccgg ttcattctca tagcgcagac cctcaggatt atagactttg tctttgccca gcaccagggt gttcttcagg gagccaccct tacccaggcc gacttttttg atgtgctcaa tttcccaatc gaaacagaaa gtgcgcgcca gaacaatttc ttcttcgtta ccctcaacaa aggtgaactt ttgacggccc agaaagttct tgaactcgcc ctcataggtg acttccaacg tgtcgctcgg ctcggcttta atcagacgac cctcatcttc cacgatgatc ggttcctcga ccacaaaata gtcgatctcg cggttttgat tcaggatatt cttacggatc gcttcgtaaa attcccaacc gctaccgtcc agaatcggga tttcgttgcc gatcacttca atcgtaacat tggtgatttc cagcagatgc agcaccgaca gaatgtgctc cacggtctta atgcgctggc ccttaaagcc cagatccgtg ctgtgattcg tgtggacaac aaattcatga cgagccggga tgtaaacacc gtttttgaag aaacgaatgc cggtgccctc tttctctgga tggataatca gcttgctgta ctcacccgta tgaataccaa caccctcgaa gctcagtttt tctttcacag ttttctccaa acccattcca ccgatttgtt cacggtgtgc ttcgataatg tcgttatctt ccatgtccag atcttccgga gtctggtctg cctgaatacg gataccgtcg tacagaaagc gcagggaatc catttccttg ccctggcgtt tagcgaatgc ttccatcagg cgacgcagcg gggtggtttt cttaatcttg aagaagattt cgctgctacc gtcgctaacc ttcaggttga tgtgggtttc cggcttcact tccggtttta cttcgccgct atggtggtgg tggtggtggg acat

Expected Protein Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMGLE KTVKEKLSFE GVGIHTGEYS KLIIHPEKEG TGIRFFKNGV YIPARHEFVV HTNHSTDLGF KGQRIKTVEH ILSVLHLLEI TNVTIEVIGN EIPILDGSGW EFYEAIRKNI LNQNREIDYF VVEEPIIVED EGRLIKAEPS DTLEVTYEGE FKNFLGRQKF TFVEGNEEEI VLARTFCFDW EIEHIKKVGL GKGGSLKNTL VLGKDKVYNP EGLRYENEPV RHKVFDLIGD LYLLGSPVKG KFYSFRGGHS LNVKLVKELA KKQKLTRDLP HLPSVQAL

Full NT Sequence (Expression Vector + Insert)

atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggaaaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaaaacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggaatg ggtttggaga aaactgtgaa agaaaaactg agcttcgagg gtgttggtat tcatacgggt gagtacagca agctgattat ccatccagag aaagagggca ccggcattcg tttcttcaaa aacggtgttt acatcccggc tcgtcatgaa tttgttgtcc acacgaatca cagcacggat ctgggcttta agggccagcg cattaagacc gtggagcaca ttctgtcggt gctgcatctg ctggaaatca ccaatgttac gattgaagtg atcggcaacg aaatcccgat tctggacggt agcggttggg aattttacga agcgatccgt aagaatatcc tgaatcaaaa ccgcgagatc gactattttg tggtcgagga accgatcatc gtggaagatg agggtcgtct gattaaagcc gagccgagcg acacgttgga agtcacctat gagggcgagt tcaagaactt tctgggccgt caaaagttca cctttgttga gggtaacgaa gaagaaattg ttctggcgcg cactttctgt ttcgattggg aaattgagca catcaaaaaa gtcggcctgg gtaagggtgg ctccctgaag aacaccctgg tgctgggcaa agacaaagtc tataatcctg agggtctgcg ctatgagaat gaaccggttc gtcacaaagt ctttgacctc atcggtgacc tgtacttgct gggttccccg gtcaagggca agttctacag cttccgtggt ggccactctt tgaacgttaa actggtgaaa gaactggcga agaaacagaa actgacccgt gatctgccgc acttaccgag cgttcaggca ctgtgataac aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtttccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtcacgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcaaggag atggcgcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatc

Details for AqaeA.00166.a.EV12.GE41552

HARVESTED ON:	6/30/2016
SEQUENCED ON:	10/25/2017
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Data Unavailable
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	TOP10
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMGLE KTVKEKLSFE GVGIHTGEYS KLIIHPEKEG TGIRFFKNGV YIPARHEFVV HTNHSTDLGF KGQRIKTVEH ILSVLHLLEI TNVTIEVIGN EIPILDGSGW EFYEAIRKNI LNQNREIDYF VVEEPIIVED EGRLIKAEPS DTLEVTYEGE FKNFLGRQKF TFVEGNEEEI VLARTFAFDW EIEHIKKVGL GKGGSLKNTL VLGKDKVYNP EGLRYENEPV RHKVFDLIGD LYLLGSPVKG KFYSFRGGHS LNVKLVKELA KKQKLTRD

Validated NT Sequence

gatcacgggt cagtttctgt ttcttcgcca gttctttcac cagtttaacg ttcaaagagt ggccaccacg gaagctgtag aacttgccct tgaccgggga acccagcaag tacaggtcac cgatgaggtc aaagactttg tgacgaaccg gttcattctc atagcgcaga ccctcaggat tatagacttt gtctttgccc agcaccaggg tgttcttcag ggagccaccc ttacccaggc cgactttttt gatgtgctca atttcccaat cgaacgcgaa agtgcgcgcc agaacaattt cttcttcgtt accctcaaca aaggtgaact tttgacggcc cagaaagttc ttgaactcgc cctcataggt gacttccaac gtgtcgctcg gctcggcttt aatcagacga ccctcatctt ccacgatgat cggttcctcg accacaaaat agtcgatctc gcggttttga ttcaggatat tcttacggat cgcttcgtaa aattcccaac cgctaccgtc cagaatcggg atttcgttgc cgatcacttc aatcgtaaca ttggtgattt ccagcagatg cagcaccgac agaatgtgct ccacggtctt aatgcgctgg cccttaaagc ccagatccgt gctgtgattc gtgtggacaa caaattcatg acgagccggg atgtaaacac cgtttttgaa gaaacgaatg ccggtgccct ctttctctgg atggataatc agcttgctgt actcacccgt atgaatacca acaccctcga agctcagttt ttctttcaca gttttctcca aacccattcc accgatttgt tcacggtgtg cttcgataat gtcgttatct tccatgtcca gatcttccgg agtctggtct gcctgaatac ggataccgtc gtacagaaag cgcagggaat ccatttcctt gccctggcgt ttagcgaatg cttccatcag gcgacgcagc ggggtggttt tcttaatctt gaagaagatt tcgctgctac cgtcgctaac cttcaggttg atgtgggttt ccggcttcac ttccggtttt acttcgccgc tatggtggtg gtggtggtgg gacat

Expected Protein Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMGLE KTVKEKLSFE GVGIHTGEYS KLIIHPEKEG TGIRFFKNGV YIPARHEFVV HTNHSTDLGF KGQRIKTVEH ILSVLHLLEI TNVTIEVIGN EIPILDGSGW EFYEAIRKNI LNQNREIDYF VVEEPIIVED EGRLIKAEPS DTLEVTYEGE FKNFLGRQKF TFVEGNEEEI VLARTFAFDW EIEHIKKVGL GKGGSLKNTL VLGKDKVYNP EGLRYENEPV RHKVFDLIGD LYLLGSPVKG KFYSFRGGHS LNVKLVKELA KKQKLTRDLP HLPSVQAL

Full NT Sequence (Expression Vector + Insert)

atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggaaaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaaaacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggaatg ggtttggaga aaactgtgaa agaaaaactg agcttcgagg gtgttggtat tcatacgggt gagtacagca agctgattat ccatccagag aaagagggca ccggcattcg tttcttcaaa aacggtgttt acatcccggc tcgtcatgaa tttgttgtcc acacgaatca cagcacggat ctgggcttta agggccagcg cattaagacc gtggagcaca ttctgtcggt gctgcatctg ctggaaatca ccaatgttac gattgaagtg atcggcaacg aaatcccgat tctggacggt agcggttggg aattttacga agcgatccgt aagaatatcc tgaatcaaaa ccgcgagatc gactattttg tggtcgagga accgatcatc gtggaagatg agggtcgtct gattaaagcc gagccgagcg acacgttgga agtcacctat gagggcgagt tcaagaactt tctgggccgt caaaagttca cctttgttga gggtaacgaa gaagaaattg ttctggcgcg cactttcgcg ttcgattggg aaattgagca catcaaaaaa gtcggcctgg gtaagggtgg ctccctgaag aacaccctgg tgctgggcaa agacaaagtc tataatcctg agggtctgcg ctatgagaat gaaccggttc gtcacaaagt ctttgacctc atcggtgacc tgtacttgct gggttccccg gtcaagggca agttctacag cttccgtggt ggccactctt tgaacgttaa actggtgaaa gaactggcga agaaacagaa actgacccgt gatctgccgc acttaccgag cgttcaggca ctgtgataac aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtttccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtcacgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcaaggag atggcgcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatc

Details for AqaeA.00166.a.JA11.GE41553

HARVESTED ON:	4/12/2017
SEQUENCED ON:	10/25/2017
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL21 DE3
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MGLEKTVKEK LSFEGVGIHT GEYSKLIIHP EKEGTGIRFF KNGVYIPARH EFVVHTNHST DLGFKGQRIK TVEHILSVLH LLEITNVTIE VIGNEIPILD GSGWEFYEAI RKNILNQNRE IDYFVVEEPI IVEDEGRLIK AEPSDTLEVT YEGEFKNFLG RQKFTFVEGN EEEIVLARTF CFDWEIEHIW KVGLGKGGSL KNTLVLGKDK VYNPEGLRYE NEPVRHKVFD LIGDLYLLGS PVKGKFYSFR GGHSLNVKLV KELAKKQKLT XDLPHLPSVQ AL

Validated NT Sequence

attcagagag cttggacgga aggaaggtga ggtaaatcan nagtaagttt ttgtttcttt gcaagctctt ttacgagttt tacgttgagc gagtgccctc ccctgaagga gtaaaactta ccttttacgg gacttccaag gaggtaaagg tctcctataa ggtcaaacac tttgtgtctt acaggttcgt tttcgtatct tagcccttca gggttgtaca ctttatcctt tccgagaacg agggtgtttt tcaggcttcc tcccttccca agaccgactt tccaaatgtg ttctatttcc cagtcaaagc aaaaagtccg cgcaaggact atttcttctt cgttcccttc aacaaaggta aacttctgcc ttcccaggaa atttttaaat tctccctcgt aggtgacctc aagcgtgtct gaaggttctg cctttatcaa tcttccctcg tcttccacga taatgggttc ttctacaaca aagtaatcta tttctctgtt ctgattgaga atatttttcc taatggcttc gtagaattcc cagccacttc cgtcaagtat agggatttcg ttccctatta cttctattgt cacgtttgtt atctcaagta ggtggagaac ggaaagtatg tgttcaaccg tttttattct ttgcccttta aagcctaaat ccgtggagtg atttgtgtga acgacgaact catgtcttgc gggtatgtaa actccgttct tgaaaaacct gattcctgtt ccttcttttt cgggatgtat gattaatttt gagtattcac ctgtgtgtat accgacaccc tcaaagctta atttctcttt aaccgtcttt tctaatccca tatggctgcc gcgcggcacc aggccgctgc tgtgatgatg atgatgatgg ctgctgcc

Expected Protein Sequence

MGSSHHHHHH SSGLVPRGSH MGLEKTVKEK LSFEGVGIHT GEYSKLIIHP EKEGTGIRFF KNGVYIPARH EFVVHTNHST DLGFKGQRIK TVEHILSVLH LLEITNVTIE VIGNEIPILD GSGWEFYEAI RKNILNQNRE IDYFVVEEPI IVEDEGRLIK AEPSDTLEVT YEGEFKNFLG RQKFTFVEGN EEEIVLARTF CFDWEIEHIW KVGLGKGGSL KNTLVLGKDK VYNPEGLRYE NEPVRHKVFD LIGDLYLLGS PVKGKFYSFR GGHSLNVKLV KELAKKQKLT RDLPHLPSVQ AL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg gattagaaaa gacggttaaa gagaaattaa gctttgaggg tgtcggtata cacacaggtg aatactcaaa attaatcata catcccgaaa aagaaggaac aggaatcagg tttttcaaga acggagttta catacccgca agacatgagt tcgtcgttca cacaaatcac tccacggatt taggctttaa agggcaaaga ataaaaacgg ttgaacacat actttccgtt ctccacctac ttgagataac aaacgtgaca atagaagtaa tagggaacga aatccctata cttgacggaa gtggctggga attctacgaa gccattagga aaaatattct caatcagaac agagaaatag attactttgt tgtagaagaa cccattatcg tggaagacga gggaagattg ataaaggcag aaccttcaga cacgcttgag gtcacctacg agggagaatt taaaaatttc ctgggaaggc agaagtttac ctttgttgaa gggaacgaag aagaaatagt ccttgcgcgg actttttgct ttgactggga aatagaacac atttggaaag tcggtcttgg gaagggagga agcctgaaaa acaccctcgt tctcggaaag gataaagtgt acaaccctga agggctaaga tacgaaaacg aacctgtaag acacaaagtg tttgacctta taggagacct ttacctcctt ggaagtcccg taaaaggtaa gttttactcc ttcaggggag ggcactcgct caacgtaaaa ctcgtaaaag agcttgcaaa gaaacaaaaa cttactcgtg atttacctca ccttccttcc gtccaagctc tctgactcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for AqaeA.00166.a.JA12.GE41554

HARVESTED ON:	4/20/2017
SEQUENCED ON:	10/25/2017
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL21 DE3
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MGLEKTVKEK LSFEGVGIHT GEYSKLIIHP EKEGTGIRFF KNGVYIPARH EFVVHTNHST DLGFKGQRIK TVEHILSVLH LLEITNVTIE VIGNEIPILD GSGWEFYEAI RKNILNQNRE IDYFVVEEPI IVEDEGRLIK AEPSDTLEVT YEGEFKNFLG RQKFTFVEGN EEEIVLARTF CFDWEIEHIW KVGLGKGSSL KNTLVLGKDK VYNPEGLRYE NEPVRHKVFD LIGDLYLLGS PVKGKFYSFR GGHSLNVKLV KELAKKQKLT RDLPHLPSVQ AL

Validated NT Sequence

attcagagag cttggacgga aggaaggtga ggtaaatcac gagtaagttt ttgtttcttt gcaagctctt ttacgagttt tacgttgagc gagtgccctc ccctgaagga gtaaaactta ccttttacgg gacttccaag gaggtaaagg tctcctataa ggtcaaacac tttgtgtctt acaggttcgt tttcgtatct tagcccttca gggttgtaca ctttatcctt tccgagaacg agggtgtttt tcaggcttga tcccttccca agaccgactt tccaaatgtg ttctatttcc cagtcaaagc aaaaagtccg cgcaaggact atttcttctt cgttcccttc aacaaaggta aacttctgcc ttcccaggaa atttttaaat tctccctcgt aggtgacctc aagcgtgtct gaaggttctg cctttatcaa tcttccctcg tcttccacga taatgggttc ttctacaaca aagtaatcta tttctctgtt ctgattgaga atatttttcc taatggcttc gtagaattcc cagccacttc cgtcaagtat agggatttcg ttccctatta cttctattgt cacgtttgtt atctcaagta ggtggagaac ggaaagtatg tgttcaaccg tttttattct ttgcccttta aagcctaaat ccgtggagtg atttgtgtga acgacgaact catgtcttgc gggtatgtaa actccgttct tgaaaaacct gattcctgtt ccttcttttt cgggatgtat gattaatttt gagtattcac ctgtgtgtat accgacaccc tcaaagctta atttctcttt aaccgtcttt tctaatccca tatggctgcc gcgcggcacc aggccgctgc tgtgatgatg atgatgatgg ctgctg

Expected Protein Sequence

MGSSHHHHHH SSGLVPRGSH MGLEKTVKEK LSFEGVGIHT GEYSKLIIHP EKEGTGIRFF KNGVYIPARH EFVVHTNHST DLGFKGQRIK TVEHILSVLH LLEITNVTIE VIGNEIPILD GSGWEFYEAI RKNILNQNRE IDYFVVEEPI IVEDEGRLIK AEPSDTLEVT YEGEFKNFLG RQKFTFVEGN EEEIVLARTF CFDWEIEHIW KVGLGKGSSL KNTLVLGKDK VYNPEGLRYE NEPVRHKVFD LIGDLYLLGS PVKGKFYSFR GGHSLNVKLV KELAKKQKLT RDLPHLPSVQ AL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg gattagaaaa gacggttaaa gagaaattaa gctttgaggg tgtcggtata cacacaggtg aatactcaaa attaatcata catcccgaaa aagaaggaac aggaatcagg tttttcaaga acggagttta catacccgca agacatgagt tcgtcgttca cacaaatcac tccacggatt taggctttaa agggcaaaga ataaaaacgg ttgaacacat actttccgtt ctccacctac ttgagataac aaacgtgaca atagaagtaa tagggaacga aatccctata cttgacggaa gtggctggga attctacgaa gccattagga aaaatattct caatcagaac agagaaatag attactttgt tgtagaagaa cccattatcg tggaagacga gggaagattg ataaaggcag aaccttcaga cacgcttgag gtcacctacg agggagaatt taaaaatttc ctgggaaggc agaagtttac ctttgttgaa gggaacgaag aagaaatagt ccttgcgcgg actttttgct ttgactggga aatagaacac atttggaaag tcggtcttgg gaagggatca agcctgaaaa acaccctcgt tctcggaaag gataaagtgt acaaccctga agggctaaga tacgaaaacg aacctgtaag acacaaagtg tttgacctta taggagacct ttacctcctt ggaagtcccg taaaaggtaa gttttactcc ttcaggggag ggcactcgct caacgtaaaa ctcgtaaaag agcttgcaaa gaaacaaaaa cttactcgtg atttacctca ccttccttcc gtccaagctc tctgactcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

CENTER ID:	AqaeA.00166.a
ORGANISM:	Aquifex aeolicus
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

AqaeA.00166.a

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (LpxC)

Ordering Clones & Proteins

Clones*

Proteins

External Resources

Sequences

AA Sequence

NT Sequence

PURIFICATION DATe:	10/23/2016
CONCENTRATION:	9.8mg/ml
OBSERVED MW:	42kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM Tris pH 7.5, 150 mM NaCl, 2 mM TCEP, 5% glycerol
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	50µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98