| CENTER ID: | AnphA.18434.a |
| ORGANISM: | Anaplasma phagocytophilum HZ |
| ASSOCIATED DISEASE: | Human granulocytic anaplasmosis |
| CURRENT STATUS: | purified |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 1 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.18434.a.B1.GE34952 | full length | 1 | 376 | ||
| AnphA.18434.a.ET11.GE00435 | full length | 26 | 217 | ||
| AnphA.18434.a.ET12.GE00436 | full length | 2 | 217 | ||
| AnphA.18434.a.ET13.GE00437 | full length | 2 | 208 |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.18434.a.ET11.PD00376 | full length | 26 | 217 | ||
| AnphA.18434.a.ET11.PD00377 | full length | 26 | 217 | ||
| AnphA.18434.a.B1.PS01494 | full length | 1 | 376 |
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|212042.8.peg.914 |
| RefSeq: | YP_505436.1 |
| UniProt: | Q2GJL5 |
| HARVESTED ON: | 3/19/2012 |
| SEQUENCED ON: | 3/22/2012 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 83 |
| HARVESTED ON: | 4/3/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 22kDa |
| OBSERVED MW: | 22kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| HARVESTED ON: | 4/3/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 24kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| HARVESTED ON: | 4/3/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 23kDa |
| OBSERVED MW: | 23kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| PURIFICATION DATe: | 6/10/2015 |
| CONCENTRATION: | 20.3mg/ml |
| OBSERVED MW: | 22kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| PURIFICATION DATe: | 6/10/2015 |
| CONCENTRATION: | 20.2mg/ml |
| OBSERVED MW: | 22kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 28 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| PURIFICATION DATe: | 5/16/2012 |
| CONCENTRATION: | 29.1mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 150µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 83 |