AnphA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID: AnphA.17987.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17987.a.B1.GE41149 full length 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1175
UniProt: Q2GJ06

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGEISRGLD FSISREDLLQ ALNYVSSVIE RRNSIAVLSC VRIEAKGSVI EFTSTDLDIS IEACVQANVA TGGVAVASAQ LLYDIVRKLS GEADVRFKLN DGKLLVACGN ARFYLHVISP EKFPDIEGSS LDCGFDIPAT SLAELLNKTK FSMSVEESRY NLRGVYFHVD DEMLCCVATD GHRLSLAKIS KPEGLHSGIA VIIPRKTVNE VLKIIGHGGG DAAQMVSVGI SNRKICFRYK GYFMVSKVLD GTFPDYAAII PTECGNSVSI DASVLQSAVD RVSVVVFDKV KAIKLSFTEG KLVLSSASSD QGDASEELSV DYEGDEVVIG LNARYLMEVL GSIKGVCEIV FSDGSAAVLV KDGEYGMHIV MPMRI
NT Sequence
atgagcggtg agataagtag agggcttgat ttcagtatca gtcgagaaga tctgctacaa gcgctgaatt atgttagcag tgttatcgag cggcgtaaca gtattgctgt tctgtcctgt gttaggatag aagctaaggg ctcagttatt gagtttactt ctacagattt agacatatca atagaagcgt gtgtacaggc caatgttgct acgggaggtg tggctgtagc gtctgcgcag ctgctgtacg atatagttag aaagctttct ggtgaagcgg acgttaggtt caaacttaat gatggcaagc tgctagtggc gtgtggaaat gcgagattct atcttcacgt tatatctccg gagaagtttc ctgacattga gggtagttcc cttgattgcg ggtttgatat acctgcgact agtcttgcgg agcttttgaa taagaccaag ttttccatgt ctgttgagga aagtaggtat aatctccggg gtgtgtattt ccatgttgat gacgaaatgc tatgttgtgt ggcaacagat ggtcatcgtt tatcgctggc gaagataagt aagcctgagg gtttgcattc tggtattgcg gttataatcc ctcgtaagac cgtgaatgag gttttgaaga tcattggcca tggtggaggc gatgcggcgc aaatggtctc tgtaggaata tctaatagga aaatatgctt taggtataag ggctacttta tggtgtccaa ggtgttggac ggtacgtttc cagactacgc agccattatc ccaacggaat gcggtaatag tgtatcaatt gacgcgagcg tattgcagag tgcagtagac cgtgtatcag tagtagtatt cgacaaggta aaggcaatca agctctcgtt tacagagggt aagttggtat taagttcagc gtcgtcagat cagggagatg caagcgaaga gttgtctgta gactatgaag gagatgaagt tgtaatagga ttaaatgcta gatacctaat ggaggtattg ggaagcatca agggtgtgtg cgaaatagta tttagcgacg gtagtgcggc ggtactggtt aaggatggcg aatatggtat gcacatcgtt atgcctatga gaatatag
Details for AnphA.17987.a.B1.GE41149
HARVESTED ON: data unavailable
SEQUENCED ON: 3/2/2017
EXPECTED MW: 41kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS GEISRGLDFS ISREDLLQAL NYVSSVIERR NSIAVLSCVR IEAKGSVIEF TSTDLDISIE ACVQANVATG GVAVASAQLL YDIVRKLSGE ADVRFKLNDG KLLVACGNAR FYLHVISPEK FPDIEGSSLD CGFDIPATSL AELLNKTKFS MSVEESRYNL RGVYFHVDDE MLCCVATDGH RLSLAKISKP EGLHSGIAVI IPRKTVNEVL KIIGHGGGDA AQMVSVGISN RKICFRYKGY FMVSKVLDGT FPDYAAIIPT ECGNSVSIDA SVLQSAVDRV SVVVFDKVKA IKLSFTEGKL VLSSASSDQG DASEELSVDY EGDEVVIGLN ARYLMEVLGS IKGVCEIVFS DGSAAVLVKD GEYGMHIVMP MRI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg gtgagataag tagagggctt gatttcagta tcagtcgaga agatctgcta caagcgctga attatgttag cagtgttatc gagcggcgta acagtattgc tgttctgtcc tgtgttagga tagaagctaa gggctcagtt attgagttta cttctacaga tttagacata tcaatagaag cgtgtgtaca ggccaatgtt gctacgggag gtgtggctgt agcgtctgcg cagctgctgt acgatatagt tagaaagctt tctggtgaag cggacgttag gttcaaactt aatgatggca agctgctagt ggcgtgtgga aatgcgagat tctatcttca cgttatatct ccggagaagt ttcctgacat tgagggtagt tcccttgatt gcgggtttga tatacctgcg actagtcttg cggagctttt gaataagacc aagttttcca tgtctgttga ggaaagtagg tataatctcc ggggtgtgta tttccatgtt gatgacgaaa tgctatgttg tgtggcaaca gatggtcatc gtttatcgct ggcgaagata agtaagcctg agggtttgca ttctggtatt gcggttataa tccctcgtaa gaccgtgaat gaggttttga agatcattgg ccatggtgga ggcgatgcgg cgcaaatggt ctctgtagga atatctaata ggaaaatatg ctttaggtat aagggctact ttatggtgtc caaggtgttg gacggtacgt ttccagacta cgcagccatt atcccaacgg aatgcggtaa tagtgtatca attgacgcga gcgtattgca gagtgcagta gaccgtgtat cagtagtagt attcgacaag gtaaaggcaa tcaagctctc gtttacagag ggtaagttgg tattaagttc agcgtcgtca gatcagggag atgcaagcga agagttgtct gtagactatg aaggagatga agttgtaata ggattaaatg ctagatacct aatggaggta ttgggaagca tcaagggtgt gtgcgaaata gtatttagcg acggtagtgc ggcggtactg gttaaggatg gcgaatatgg tatgcacatc gttatgccta tgagaatatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat