AnphA.17482.a

GTP-binding protein YchF

CENTER ID: AnphA.17482.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17482.a.A1.GE30784 Full length( AnphA.17482.a ) 1 363
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17482.a.A1.PW30671 Full length( AnphA.17482.a ) 1 363

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1376
RefSeq: YP_505800.1
UniProt: Q2GIK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGLNCGIVGL PNVGKSTLFN ALTQTTLAEV ANYPFCTIEP NAGRTIVRDA RLKTLASMAG SQNTIFSQVE FVDIAGLVRG ASAGEGLGNK FLGHIREVDA IMHVLRCFED EDIQHVNNAV NPVHDAEIVE MELIMADMES VKRRIMNVAK AAKSDKTQHS QKELLEKILA ALEDGRPARS VADVAPQELK HLQLLTSKPV MYVCNVEDTN AATGNALVEA VKEMAQRSGS HCCYLSAKLE TDILSFEDEE MRSNFLEELG MAESGIDTAV RTIYNLLDLI TFFTLGPKEA RAWSIMRNTP ADKAAGTIHT DFEKGFIKAE TISFEDYIQC GGEAECKKAG KVRFEGRDYI VQDGDIMHFR VNK
NT Sequence
ATGGGACTAA ACTGCGGGAT AGTAGGCCTA CCTAATGTTG GGAAGTCTAC ATTATTTAAT GCTCTAACAC AAACCACGCT TGCAGAGGTG GCTAACTATC CGTTCTGTAC GATAGAGCCT AATGCGGGCA GGACTATTGT CAGAGATGCT AGACTCAAAA CCTTGGCATC CATGGCAGGT TCGCAGAATA CTATTTTCAG CCAGGTAGAA TTCGTTGATA TTGCGGGTTT GGTGCGTGGG GCGAGTGCAG GAGAAGGTTT GGGGAACAAG TTTCTCGGCC ATATCCGGGA AGTTGATGCC ATTATGCACG TTTTACGGTG TTTTGAAGAT GAAGATATAC AGCATGTGAA TAATGCTGTT AACCCAGTGC ATGATGCAGA AATCGTGGAA ATGGAACTGA TCATGGCTGA TATGGAAAGC GTGAAGCGTC GCATTATGAA CGTTGCCAAA GCAGCTAAGT CCGACAAAAC ACAACATAGT CAAAAGGAAC TACTAGAAAA GATTTTAGCA GCTTTGGAGG ACGGTAGACC TGCAAGATCT GTAGCGGATG TTGCTCCCCA GGAGCTTAAG CACTTGCAGC TTTTAACTAG CAAACCCGTT ATGTACGTAT GTAATGTGGA GGACACTAAT GCGGCTACTG GTAATGCCCT AGTTGAGGCT GTAAAAGAGA TGGCGCAACG CTCTGGCAGC CATTGCTGTT ATCTTTCGGC GAAGCTAGAA ACAGATATAC TCAGCTTCGA AGATGAAGAG ATGAGAAGTA ATTTTCTTGA AGAGCTGGGT ATGGCGGAAT CGGGTATCGA TACAGCGGTA AGAACAATTT ACAATTTATT AGATCTCATA ACTTTCTTTA CCCTAGGCCC CAAGGAGGCG CGTGCGTGGT CTATAATGCG CAATACGCCC GCGGATAAAG CTGCTGGAAC TATACACACC GACTTTGAAA AGGGTTTCAT AAAGGCTGAA ACTATAAGCT TTGAGGACTA CATTCAATGT GGGGGTGAGG CTGAATGTAA AAAGGCTGGT AAGGTACGGT TTGAAGGCAG AGATTATATA GTACAAGATG GGGACATAAT GCACTTTAGA GTCAACAAG
Details for AnphA.17482.a.A1.GE30784
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MGLNCGIVGL PNVGKSTLFN ALTQTTLAEV ANYPFCTIEP NAGRTIVRDA RLKTLASMAG SQNTIFSQVE FVDIAGLVRG ASAGEGLGNK FLGHIREVDA IMHVLRCFED EDIQHVNNAV NPVHDAEIVE MELIMADMES VKRRIMNVAK AAKSDKTQHS QKELLEKILA ALEDGRPARS VADVAPQELK HLQLLTSKPV MYVCNVEDTN AATGNALVEA VKEMAQRSGS HCCYLSAKLE TDILSFEDEE MRSNFLEELG MAESGIDTAV RTIYNLLDLI TFFTLGPKEA RAWSIMRNTP ADKAAGTIHT DFEKGFIKAE TISFEDYIQC GGEAECKKAG KVRFEGRDYI VQDGDIMHFR V
Validated NT Sequence
tgactctaaa gtgcattatg tccccatctt gtactatata atctctgcct tcaaaccgta ccttaccagc ctttttacat tcagcctcac ccccacattg aatgtagtcc tcaaagctta tagtttcagc ctttatgaaa cccttttcaa agtcggtgtg tatagttcca gcagctttat ccgcgggcgt attgcgcatt atagaccacg cacgcgcctc cttggggcct agggtaaaga aagttatgag atctaataaa ttgtaaattg ttcttaccgc tgtatcgata cccgattccg ccatacccag ctcttcaaga aaattacttc tcatctcttc atcttcgaag ctgagtatat ctgtttctag cttcgccgaa agataacagc aatggctgcc agagcgttgc gccatctctt ttacagcctc aactagggca ttaccagtag ccgcattagt gtcctccaca ttacatacgt acataacggg tttgctagtt aaaagctgca agtgcttaag ctcctgggga gcaacatccg ctacagatct tgcaggtcta ccgtcctcca aagctgctaa aatcttttct agtagttcct tttgactatg ttgtgttttg tcggacttag ctgctttggc aacgttcata atgcgacgct tcacgctttc catatcagcc atgatcagtt ccatttccac gatttctgca tcatgcactg ggttaacagc attattcaca tgctgtatat cttcatcttc aaaacaccgt aaaacgtgca taatggcatc aacttcccgg atatggccga gaaacttgtt ccccaaacct tctcctgcac tcgccccacg caccaaaccc gcaatatcaa cgaattctac ctggctgaaa atagtattct gcgaacctgc catggatgcc aaggttttga gtctagcatc tctgacaata gtcctgcccg cattaggctc tatcgtacag aacggatagt tagccacctc tgcaagcgtg gtttgtgtta gagcattaaa taatgtagac ttcccaacat taggtaggcc tactatcccg cagtttagtc ccatcgaacc aggaccctgg gtctgagctt ccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLNCGIVG LPNVGKSTLF NALTQTTLAE VANYPFCTIE PNAGRTIVRD ARLKTLASMA GSQNTIFSQV EFVDIAGLVR GASAGEGLGN KFLGHIREVD AIMHVLRCFE DEDIQHVNNA VNPVHDAEIV EMELIMADME SVKRRIMNVA KAAKSDKTQH SQKELLEKIL AALEDGRPAR SVADVAPQEL KHLQLLTSKP VMYVCNVEDT NAATGNALVE AVKEMAQRSG SHCCYLSAKL ETDILSFEDE EMRSNFLEEL GMAESGIDTA VRTIYNLLDL ITFFTLGPKE ARAWSIMRNT PADKAAGTIH TDFEKGFIKA ETISFEDYIQ CGGEAECKKA GKVRFEGRDY IVQDGDIMHF RVNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggactaaac tgcgggatag taggcctacc taatgttggg aagtctacat tatttaatgc tctaacacaa accacgcttg cagaggtggc taactatccg ttctgtacga tagagcctaa tgcgggcagg actattgtca gagatgctag actcaaaacc ttggcatcca tggcaggttc gcagaatact attttcagcc aggtagaatt cgttgatatt gcgggtttgg tgcgtggggc gagtgcagga gaaggtttgg ggaacaagtt tctcggccat atccgggaag ttgatgccat tatgcacgtt ttacggtgtt ttgaagatga agatatacag catgtgaata atgctgttaa cccagtgcat gatgcagaaa tcgtggaaat ggaactgatc atggctgata tggaaagcgt gaagcgtcgc attatgaacg ttgccaaagc agctaagtcc gacaaaacac aacatagtca aaaggaacta ctagaaaaga ttttagcagc tttggaggac ggtagacctg caagatctgt agcggatgtt gctccccagg agcttaagca cttgcagctt ttaactagca aacccgttat gtacgtatgt aatgtggagg acactaatgc ggctactggt aatgccctag ttgaggctgt aaaagagatg gcgcaacgct ctggcagcca ttgctgttat ctttcggcga agctagaaac agatatactc agcttcgaag atgaagagat gagaagtaat tttcttgaag agctgggtat ggcggaatcg ggtatcgata cagcggtaag aacaatttac aatttattag atctcataac tttctttacc ctaggcccca aggaggcgcg tgcgtggtct ataatgcgca atacgcccgc ggataaagct gctggaacta tacacaccga ctttgaaaag ggtttcataa aggctgaaac tataagcttt gaggactaca ttcaatgtgg gggtgaggct gaatgtaaaa aggctggtaa ggtacggttt gaaggcagag attatatagt acaagatggg gacataatgc actttagagt caacaagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for AnphA.17482.a.A1.PW30671
PURIFICATION DATe: 2/11/2011
CONCENTRATION: 28.26mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MGLNCGIVGL PNVGKSTLFN ALTQTTLAEV ANYPFCTIEP NAGRTIVRDA RLKTLASMAG SQNTIFSQVE FVDIAGLVRG ASAGEGLGNK FLGHIREVDA IMHVLRCFED EDIQHVNNAV NPVHDAEIVE MELIMADMES VKRRIMNVAK AAKSDKTQHS QKELLEKILA ALEDGRPARS VADVAPQELK HLQLLTSKPV MYVCNVEDTN AATGNALVEA VKEMAQRSGS HCCYLSAKLE TDILSFEDEE MRSNFLEELG MAESGIDTAV RTIYNLLDLI TFFTLGPKEA RAWSIMRNTP ADKAAGTIHT DFEKGFIKAE TISFEDYIQC GGEAECKKAG KVRFEGRDYI VQDGDIMHFR V
Validated NT Sequence
tgactctaaa gtgcattatg tccccatctt gtactatata atctctgcct tcaaaccgta ccttaccagc ctttttacat tcagcctcac ccccacattg aatgtagtcc tcaaagctta tagtttcagc ctttatgaaa cccttttcaa agtcggtgtg tatagttcca gcagctttat ccgcgggcgt attgcgcatt atagaccacg cacgcgcctc cttggggcct agggtaaaga aagttatgag atctaataaa ttgtaaattg ttcttaccgc tgtatcgata cccgattccg ccatacccag ctcttcaaga aaattacttc tcatctcttc atcttcgaag ctgagtatat ctgtttctag cttcgccgaa agataacagc aatggctgcc agagcgttgc gccatctctt ttacagcctc aactagggca ttaccagtag ccgcattagt gtcctccaca ttacatacgt acataacggg tttgctagtt aaaagctgca agtgcttaag ctcctgggga gcaacatccg ctacagatct tgcaggtcta ccgtcctcca aagctgctaa aatcttttct agtagttcct tttgactatg ttgtgttttg tcggacttag ctgctttggc aacgttcata atgcgacgct tcacgctttc catatcagcc atgatcagtt ccatttccac gatttctgca tcatgcactg ggttaacagc attattcaca tgctgtatat cttcatcttc aaaacaccgt aaaacgtgca taatggcatc aacttcccgg atatggccga gaaacttgtt ccccaaacct tctcctgcac tcgccccacg caccaaaccc gcaatatcaa cgaattctac ctggctgaaa atagtattct gcgaacctgc catggatgcc aaggttttga gtctagcatc tctgacaata gtcctgcccg cattaggctc tatcgtacag aacggatagt tagccacctc tgcaagcgtg gtttgtgtta gagcattaaa taatgtagac ttcccaacat taggtaggcc tactatcccg cagtttagtc ccatcgaacc aggaccctgg gtctgagctt ccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLNCGIVG LPNVGKSTLF NALTQTTLAE VANYPFCTIE PNAGRTIVRD ARLKTLASMA GSQNTIFSQV EFVDIAGLVR GASAGEGLGN KFLGHIREVD AIMHVLRCFE DEDIQHVNNA VNPVHDAEIV EMELIMADME SVKRRIMNVA KAAKSDKTQH SQKELLEKIL AALEDGRPAR SVADVAPQEL KHLQLLTSKP VMYVCNVEDT NAATGNALVE AVKEMAQRSG SHCCYLSAKL ETDILSFEDE EMRSNFLEEL GMAESGIDTA VRTIYNLLDL ITFFTLGPKE ARAWSIMRNT PADKAAGTIH TDFEKGFIKA ETISFEDYIQ CGGEAECKKA GKVRFEGRDY IVQDGDIMHF RVNK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGACTAAAC TGCGGGATAG TAGGCCTACC TAATGTTGGG AAGTCTACAT TATTTAATGC TCTAACACAA ACCACGCTTG CAGAGGTGGC TAACTATCCG TTCTGTACGA TAGAGCCTAA TGCGGGCAGG ACTATTGTCA GAGATGCTAG ACTCAAAACC TTGGCATCCA TGGCAGGTTC GCAGAATACT ATTTTCAGCC AGGTAGAATT CGTTGATATT GCGGGTTTGG TGCGTGGGGC GAGTGCAGGA GAAGGTTTGG GGAACAAGTT TCTCGGCCAT ATCCGGGAAG TTGATGCCAT TATGCACGTT TTACGGTGTT TTGAAGATGA AGATATACAG CATGTGAATA ATGCTGTTAA CCCAGTGCAT GATGCAGAAA TCGTGGAAAT GGAACTGATC ATGGCTGATA TGGAAAGCGT GAAGCGTCGC ATTATGAACG TTGCCAAAGC AGCTAAGTCC GACAAAACAC AACATAGTCA AAAGGAACTA CTAGAAAAGA TTTTAGCAGC TTTGGAGGAC GGTAGACCTG CAAGATCTGT AGCGGATGTT GCTCCCCAGG AGCTTAAGCA CTTGCAGCTT TTAACTAGCA AACCCGTTAT GTACGTATGT AATGTGGAGG ACACTAATGC GGCTACTGGT AATGCCCTAG TTGAGGCTGT AAAAGAGATG GCGCAACGCT CTGGCAGCCA TTGCTGTTAT CTTTCGGCGA AGCTAGAAAC AGATATACTC AGCTTCGAAG ATGAAGAGAT GAGAAGTAAT TTTCTTGAAG AGCTGGGTAT GGCGGAATCG GGTATCGATA CAGCGGTAAG AACAATTTAC AATTTATTAG ATCTCATAAC TTTCTTTACC CTAGGCCCCA AGGAGGCGCG TGCGTGGTCT ATAATGCGCA ATACGCCCGC GGATAAAGCT GCTGGAACTA TACACACCGA CTTTGAAAAG GGTTTCATAA AGGCTGAAAC TATAAGCTTT GAGGACTACA TTCAATGTGG GGGTGAGGCT GAATGTAAAA AGGCTGGTAA GGTACGGTTT GAAGGCAGAG ATTATATAGT ACAAGATGGG GACATAATGC ACTTTAGAGT CAACAAGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA