AnphA.17384.a

Type IV secretion system protein VirB9

CENTER ID: AnphA.17384.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17384.a.A2.GE34597 Mature protein( AnphA.17384.a ) 20 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17384.a.A2.PW35207 Mature protein( AnphA.17384.a ) 20 271

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.87
RefSeq: YP_504712.1
UniProt: Q2GLN9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSALLIFVV VLLYSSAGLS KQEPRSIAAD DHIKTLNYNP QSIHKYTGFY GYQSSILFEA GETISTVSMG DSTGWQLVPQ GNRLFIKPVA DNADTNVTIL TNRRVYYFEL HAEEASGLDD PRLAYEVRLV YPSLEGSIGG NVSAVGGDVL FPSYQTDVPD LRNPDVARKG LNFNYTVSHT PGSEDIVPLR VFDDGKFTYM QFSGVNGDLP SVFNVDAQGY ESLINFRMVG DYVVIERTSR AFTLRYGSST ACIFNEKRAF PVVKGAERRR K
NT Sequence
ATGAGAAGTG CTTTACTGAT TTTCGTTGTA GTGTTGCTCT ACAGTTCGGC TGGACTTTCT AAACAAGAGC CACGTTCTAT AGCGGCTGAT GATCACATTA AGACCTTGAA CTATAATCCC CAGTCTATTC ACAAATACAC CGGATTTTAC GGGTATCAGT CTAGTATCTT ATTTGAGGCA GGGGAAACGA TTTCCACAGT GTCTATGGGT GATTCAACAG GTTGGCAGCT TGTACCACAG GGTAATAGGT TGTTTATCAA ACCTGTCGCT GACAACGCAG ATACAAACGT TACTATTCTG ACCAATAGAC GCGTTTATTA TTTTGAGTTA CACGCTGAAG AGGCTTCCGG GTTGGACGAT CCTAGGCTGG CTTATGAAGT TCGCCTTGTA TACCCTAGCT TGGAGGGAAG TATCGGCGGC AACGTCTCTG CTGTTGGTGG AGATGTATTA TTTCCATCGT ACCAAACTGA TGTACCGGAC TTAAGGAATC CAGACGTCGC GAGGAAGGGG CTGAATTTTA ATTACACTGT ATCTCATACT CCGGGCTCAG AGGATATTGT GCCCCTTAGG GTATTTGACG ACGGTAAGTT TACGTATATG CAGTTTTCTG GCGTTAATGG TGATTTACCA TCAGTCTTCA ACGTAGATGC GCAAGGATAT GAGTCCTTGA TAAATTTTAG AATGGTCGGA GATTATGTGG TCATTGAACG TACATCTAGA GCATTTACTC TACGGTACGG CTCTAGCACA GCTTGTATAT TCAATGAGAA AAGGGCATTC CCTGTAGTGA AGGGTGCTGA AAGACGCCGG AAA
Details for AnphA.17384.a.A2.GE34597
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKQEPRSI AADDHIKTLN YNPQSIHKYT GFYGYQSSIL FEAGETISTV SMGDSTGWQL VPQGNRLFIK PVADNADTNV TILTNRRVYY FELHAEEASG LDDPRLAYEV RLVYPSLEGS IGGNVSAVGG DVLFPSYQTD VPDLRNPDVA RKGLNFNYTV SHTPGSEDIV PLRVFDDGKF TYMQFSGVNG DLPSVFNVDA QGYESLINFR MVGDYVVIER TSRAFTLRYG SSTACIFNEK RAFPVVKGAE RRRK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcta aacaagagcc acgttctata gcggctgatg atcacattaa gaccttgaac tataatcccc agtctattca caaatacacc ggattttacg ggtatcagtc tagtatctta tttgaggcag gggaaacgat ttccacagtg tctatgggtg attcaacagg ttggcagctt gtaccacagg gtaataggtt gtttatcaaa cctgtcgctg acaacgcaga tacaaacgtt actattctga ccaatagacg cgtttattat tttgagttac acgctgaaga ggcttccggg ttggacgatc ctaggctggc ttatgaagtt cgccttgtat accctagctt ggagggaagt atcggcggca acgtctctgc tgttggtgga gatgtattat ttccatcgta ccaaactgat gtaccggact taaggaatcc agacgtcgcg aggaaggggc tgaattttaa ttacactgta tctcatactc cgggctcaga ggatattgtg ccccttaggg tatttgacga cggtaagttt acgtatatgc agttttctgg cgttaatggt gatttaccat cagtcttcaa cgtagatgcg caaggatatg agtccttgat aaattttaga atggtcggag attatgtggt cattgaacgt acatctagag catttactct acggtacggc tctagcacag cttgtatatt caatgagaaa agggcattcc ctgtagtgaa gggtgctgaa agacgccgga aataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKQEPRSI AADDHIKTLN YNPQSIHKYT GFYGYQSSIL FEAGETISTV SMGDSTGWQL VPQGNRLFIK PVADNADTNV TILTNRRVYY FELHAEEASG LDDPRLAYEV RLVYPSLEGS IGGNVSAVGG DVLFPSYQTD VPDLRNPDVA RKGLNFNYTV SHTPGSEDIV PLRVFDDGKF TYMQFSGVNG DLPSVFNVDA QGYESLINFR MVGDYVVIER TSRAFTLRYG SSTACIFNEK RAFPVVKGAE RRRK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctaaacaa gagccacgtt ctatagcggc tgatgatcac attaagacct tgaactataa tccccagtct attcacaaat acaccggatt ttacgggtat cagtctagta tcttatttga ggcaggggaa acgatttcca cagtgtctat gggtgattca acaggttggc agcttgtacc acagggtaat aggttgttta tcaaacctgt cgctgacaac gcagatacaa acgttactat tctgaccaat agacgcgttt attattttga gttacacgct gaagaggctt ccgggttgga cgatcctagg ctggcttatg aagttcgcct tgtataccct agcttggagg gaagtatcgg cggcaacgtc tctgctgttg gtggagatgt attatttcca tcgtaccaaa ctgatgtacc ggacttaagg aatccagacg tcgcgaggaa ggggctgaat tttaattaca ctgtatctca tactccgggc tcagaggata ttgtgcccct tagggtattt gacgacggta agtttacgta tatgcagttt tctggcgtta atggtgattt accatcagtc ttcaacgtag atgcgcaagg atatgagtcc ttgataaatt ttagaatggt cggagattat gtggtcattg aacgtacatc tagagcattt actctacggt acggctctag cacagcttgt atattcaatg agaaaagggc attccctgta gtgaagggtg ctgaaagacg ccggaaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for AnphA.17384.a.A2.PW35207
PURIFICATION DATe: 3/9/2012
CONCENTRATION: 27.09mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKQEPRSI AADDHIKTLN YNPQSIHKYT GFYGYQSSIL FEAGETISTV SMGDSTGWQL VPQGNRLFIK PVADNADTNV TILTNRRVYY FELHAEEASG LDDPRLAYEV RLVYPSLEGS IGGNVSAVGG DVLFPSYQTD VPDLRNPDVA RKGLNFNYTV SHTPGSEDIV PLRVFDDGKF TYMQFSGVNG DLPSVFNVDA QGYESLINFR MVGDYVVIER TSRAFTLRYG SSTACIFNEK RAFPVVKGAE RRRK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcta aacaagagcc acgttctata gcggctgatg atcacattaa gaccttgaac tataatcccc agtctattca caaatacacc ggattttacg ggtatcagtc tagtatctta tttgaggcag gggaaacgat ttccacagtg tctatgggtg attcaacagg ttggcagctt gtaccacagg gtaataggtt gtttatcaaa cctgtcgctg acaacgcaga tacaaacgtt actattctga ccaatagacg cgtttattat tttgagttac acgctgaaga ggcttccggg ttggacgatc ctaggctggc ttatgaagtt cgccttgtat accctagctt ggagggaagt atcggcggca acgtctctgc tgttggtgga gatgtattat ttccatcgta ccaaactgat gtaccggact taaggaatcc agacgtcgcg aggaaggggc tgaattttaa ttacactgta tctcatactc cgggctcaga ggatattgtg ccccttaggg tatttgacga cggtaagttt acgtatatgc agttttctgg cgttaatggt gatttaccat cagtcttcaa cgtagatgcg caaggatatg agtccttgat aaattttaga atggtcggag attatgtggt cattgaacgt acatctagag catttactct acggtacggc tctagcacag cttgtatatt caatgagaaa agggcattcc ctgtagtgaa gggtgctgaa agacgccgga aataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKQEPRSI AADDHIKTLN YNPQSIHKYT GFYGYQSSIL FEAGETISTV SMGDSTGWQL VPQGNRLFIK PVADNADTNV TILTNRRVYY FELHAEEASG LDDPRLAYEV RLVYPSLEGS IGGNVSAVGG DVLFPSYQTD VPDLRNPDVA RKGLNFNYTV SHTPGSEDIV PLRVFDDGKF TYMQFSGVNG DLPSVFNVDA QGYESLINFR MVGDYVVIER TSRAFTLRYG SSTACIFNEK RAFPVVKGAE RRRK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTAAACAA GAGCCACGTT CTATAGCGGC TGATGATCAC ATTAAGACCT TGAACTATAA TCCCCAGTCT ATTCACAAAT ACACCGGATT TTACGGGTAT CAGTCTAGTA TCTTATTTGA GGCAGGGGAA ACGATTTCCA CAGTGTCTAT GGGTGATTCA ACAGGTTGGC AGCTTGTACC ACAGGGTAAT AGGTTGTTTA TCAAACCTGT CGCTGACAAC GCAGATACAA ACGTTACTAT TCTGACCAAT AGACGCGTTT ATTATTTTGA GTTACACGCT GAAGAGGCTT CCGGGTTGGA CGATCCTAGG CTGGCTTATG AAGTTCGCCT TGTATACCCT AGCTTGGAGG GAAGTATCGG CGGCAACGTC TCTGCTGTTG GTGGAGATGT ATTATTTCCA TCGTACCAAA CTGATGTACC GGACTTAAGG AATCCAGACG TCGCGAGGAA GGGGCTGAAT TTTAATTACA CTGTATCTCA TACTCCGGGC TCAGAGGATA TTGTGCCCCT TAGGGTATTT GACGACGGTA AGTTTACGTA TATGCAGTTT TCTGGCGTTA ATGGTGATTT ACCATCAGTC TTCAACGTAG ATGCGCAAGG ATATGAGTCC TTGATAAATT TTAGAATGGT CGGAGATTAT GTGGTCATTG AACGTACATC TAGAGCATTT ACTCTACGGT ACGGCTCTAG CACAGCTTGT ATATTCAATG AGAAAAGGGC ATTCCCTGTA GTGAAGGGTG CTGAAAGACG CCGGAAATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA