AnphA.17383.a

Type IV secretion system protein VirB9

CENTER ID: AnphA.17383.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17383.a.A2.GE30775 Mature protein( AnphA.17383.a ) 27 281
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17383.a.A2.PS00998 Mature protein( AnphA.17383.a ) 27 281

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1500
RefSeq: YP_505897.1
UniProt: Q2GIA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMNFYKNFYV ALVTAFALFS MSKACFASTN IGVPVSVDSR IKTFVYSQNE VFPVVFNYGY HSYIEFSQGE TVRVMALGDN ANWKIRPVDN KLYVMPLEKE GHTNMLIETS KGRSYAFDLI STAIPLSGGA ASSINKLGKT NSALADLAYV VRFYYPQSDR NFDIMGQKLE ISPPSLASSL DADDVEIEPN ATRTNYMFTG GSAHVSLAPT QAFDDGYLTY FQFGKNNKEI PKIYVVKKDG KKVPCKMLLL RDYVIVEGVH ELFYLDFGDG RSVEVVNQAL S
NT Sequence
ATGATGAATT TCTATAAAAA TTTTTATGTT GCTTTGGTAA CGGCTTTCGC GCTGTTCTCT ATGAGTAAAG CATGCTTTGC CAGCACAAAT ATTGGCGTAC CAGTTTCTGT AGATAGTAGA ATAAAGACAT TTGTCTACAG TCAGAATGAG GTTTTTCCGG TTGTGTTCAA CTATGGCTAT CATTCCTACA TAGAATTCTC GCAAGGTGAG ACGGTGCGAG TTATGGCTTT AGGAGATAAT GCAAATTGGA AGATAAGGCC GGTGGACAAC AAGTTATACG TCATGCCCTT GGAAAAAGAG GGGCACACTA ATATGCTCAT AGAAACGAGT AAGGGGCGGA GTTACGCTTT TGATTTGATA TCGACTGCGA TTCCTCTGAG TGGAGGTGCT GCATCTAGTA TTAACAAGTT GGGAAAGACT AATTCTGCAT TAGCAGACTT AGCTTATGTA GTGCGTTTTT ACTATCCGCA GAGTGATAGA AATTTTGATA TCATGGGACA GAAACTTGAG ATATCTCCTC CTAGCCTGGC TAGCAGTTTA GATGCTGATG ATGTGGAAAT AGAGCCAAAT GCCACTAGAA CCAACTATAT GTTTACTGGT GGAAGTGCTC ACGTTTCTCT AGCTCCTACG CAGGCTTTTG ATGATGGATA TCTGACATAC TTTCAGTTTG GTAAAAATAA CAAAGAAATT CCCAAAATCT ACGTTGTGAA GAAGGATGGA AAAAAAGTTC CATGTAAAAT GCTGCTTCTT AGGGACTATG TAATAGTTGA GGGGGTGCAT GAGCTGTTTT ACCTTGATTT TGGTGATGGC AGGAGTGTAG AAGTTGTGAA TCAGGCTCTT AGT
Details for AnphA.17383.a.A2.GE30775
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 74
Validated AA Sequence
MALGDNANWK IRPVDNKLYV MPLEKEGHTN MLIETSKGRS YAFDLISTAI PLSGGAASSI NKLGKTNSAL ADLAYVVRFY YPQSDRNFDI MGQKLEISPP SLASSLDADD VEIEPNATRT NYMFTGGSAH VSLAPTQAFD DGYLTYFQFG KNNKEIPKIY VVKKDGKKVP CKMLLLRDYV IVEGVHELFY LDFGDGRSVE VVNQALS
Validated NT Sequence
ttattaacta agagcctgat tcacaacttc tacactcctg ccatcaccaa aatcaaggta aaacagctca tgcaccccct caactattac atagtcccta agaagcagca ttttacatgg aacttttttt ccatccttct tcacaacgta gattttggga atttctttgt tatttttacc aaactgaaag tatgtcagat atccatcatc aaaagcctgc gtaggagcta gagaaacgtg agcacttcca ccagtaaaca tatagttggt tctagtggca tttggctcta tttccacatc atcagcatct aaactgctag ccaggctagg aggagatatc tcaagtttct gtcccatgat atcaaaattt ctatcactct gcggatagta aaaacgcact acataagcta agtctgctaa tgcagaatta gtctttccca acttgttaat actagatgca gcacctccac tcagaggaat cgcagtcgat atcaaatcaa aagcgtaact ccgcccctta ctcgtttcta tgagcatatt agtgtgcccc tctttttcca agggcatgac gtataacttg ttgtccaccg gccttatctt ccaatttgca ttatctccta aagccataac tcgcaccgtc tcaccttgcg agaattctat gtaggaatga tagccatagt tgaacacaac cggaaaaacc tcattctgac tgtagacaaa tgtctttatt ctactatcta cagaaactgg tacg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASTNIGVP VSVDSRIKTF VYSQNEVFPV VFNYGYHSYI EFSQGETVRV MALGDNANWK IRPVDNKLYV MPLEKEGHTN MLIETSKGRS YAFDLISTAI PLSGGAASSI NKLGKTNSAL ADLAYVVRFY YPQSDRNFDI MGQKLEISPP SLASSLDADD VEIEPNATRT NYMFTGGSAH VSLAPTQAFD DGYLTYFQFG KNNKEIPKIY VVKKDGKKVP CKMLLLRDYV IVEGVHELFY LDFGDGRSVE VVNQALS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccagcaca aatattggcg taccagtttc tgtagatagt agaataaaga catttgtcta cagtcagaat gaggtttttc cggttgtgtt caactatggc tatcattcct acatagaatt ctcgcaaggt gagacggtgc gagttatggc tttaggagat aatgcaaatt ggaagataag gccggtggac aacaagttat acgtcatgcc cttggaaaaa gaggggcaca ctaatatgct catagaaacg agtaaggggc ggagttacgc ttttgatttg atatcgactg cgattcctct gagtggaggt gctgcatcta gtattaacaa gttgggaaag actaattctg cattagcaga cttagcttat gtagtgcgtt tttactatcc gcagagtgat agaaattttg atatcatggg acagaaactt gagatatctc ctcctagcct ggctagcagt ttagatgctg atgatgtgga aatagagcca aatgccacta gaaccaacta tatgtttact ggtggaagtg ctcacgtttc tctagctcct acgcaggctt ttgatgatgg atatctgaca tactttcagt ttggtaaaaa taacaaagaa attcccaaaa tctacgttgt gaagaaggat ggaaaaaaag ttccatgtaa aatgctgctt cttagggact atgtaatagt tgagggggtg catgagctgt tttaccttga ttttggtgat ggcaggagtg tagaagttgt gaatcaggct cttagttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for AnphA.17383.a.A2.PS00998
PURIFICATION DATe: 3/9/2011
CONCENTRATION: 10.28mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 74
Protocol Notes
notes unavailable
Validated AA Sequence
MALGDNANWK IRPVDNKLYV MPLEKEGHTN MLIETSKGRS YAFDLISTAI PLSGGAASSI NKLGKTNSAL ADLAYVVRFY YPQSDRNFDI MGQKLEISPP SLASSLDADD VEIEPNATRT NYMFTGGSAH VSLAPTQAFD DGYLTYFQFG KNNKEIPKIY VVKKDGKKVP CKMLLLRDYV IVEGVHELFY LDFGDGRSVE VVNQALS
Validated NT Sequence
ttattaacta agagcctgat tcacaacttc tacactcctg ccatcaccaa aatcaaggta aaacagctca tgcaccccct caactattac atagtcccta agaagcagca ttttacatgg aacttttttt ccatccttct tcacaacgta gattttggga atttctttgt tatttttacc aaactgaaag tatgtcagat atccatcatc aaaagcctgc gtaggagcta gagaaacgtg agcacttcca ccagtaaaca tatagttggt tctagtggca tttggctcta tttccacatc atcagcatct aaactgctag ccaggctagg aggagatatc tcaagtttct gtcccatgat atcaaaattt ctatcactct gcggatagta aaaacgcact acataagcta agtctgctaa tgcagaatta gtctttccca acttgttaat actagatgca gcacctccac tcagaggaat cgcagtcgat atcaaatcaa aagcgtaact ccgcccctta ctcgtttcta tgagcatatt agtgtgcccc tctttttcca agggcatgac gtataacttg ttgtccaccg gccttatctt ccaatttgca ttatctccta aagccataac tcgcaccgtc tcaccttgcg agaattctat gtaggaatga tagccatagt tgaacacaac cggaaaaacc tcattctgac tgtagacaaa tgtctttatt ctactatcta cagaaactgg tacg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASTNIGVP VSVDSRIKTF VYSQNEVFPV VFNYGYHSYI EFSQGETVRV MALGDNANWK IRPVDNKLYV MPLEKEGHTN MLIETSKGRS YAFDLISTAI PLSGGAASSI NKLGKTNSAL ADLAYVVRFY YPQSDRNFDI MGQKLEISPP SLASSLDADD VEIEPNATRT NYMFTGGSAH VSLAPTQAFD DGYLTYFQFG KNNKEIPKIY VVKKDGKKVP CKMLLLRDYV IVEGVHELFY LDFGDGRSVE VVNQALS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCAGCACA AATATTGGCG TACCAGTTTC TGTAGATAGT AGAATAAAGA CATTTGTCTA CAGTCAGAAT GAGGTTTTTC CGGTTGTGTT CAACTATGGC TATCATTCCT ACATAGAATT CTCGCAAGGT GAGACGGTGC GAGTTATGGC TTTAGGAGAT AATGCAAATT GGAAGATAAG GCCGGTGGAC AACAAGTTAT ACGTCATGCC CTTGGAAAAA GAGGGGCACA CTAATATGCT CATAGAAACG AGTAAGGGGC GGAGTTACGC TTTTGATTTG ATATCGACTG CGATTCCTCT GAGTGGAGGT GCTGCATCTA GTATTAACAA GTTGGGAAAG ACTAATTCTG CATTAGCAGA CTTAGCTTAT GTAGTGCGTT TTTACTATCC GCAGAGTGAT AGAAATTTTG ATATCATGGG ACAGAAACTT GAGATATCTC CTCCTAGCCT GGCTAGCAGT TTAGATGCTG ATGATGTGGA AATAGAGCCA AATGCCACTA GAACCAACTA TATGTTTACT GGTGGAAGTG CTCACGTTTC TCTAGCTCCT ACGCAGGCTT TTGATGATGG ATATCTGACA TACTTTCAGT TTGGTAAAAA TAACAAAGAA ATTCCCAAAA TCTACGTTGT GAAGAAGGAT GGAAAAAAAG TTCCATGTAA AATGCTGCTT CTTAGGGACT ATGTAATAGT TGAGGGGGTG CATGAGCTGT TTTACCTTGA TTTTGGTGAT GGCAGGAGTG TAGAAGTTGT GAATCAGGCT CTTAGTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA