SSGCID: Target Page

AnphA.17381.a

Type IV secretion system protein VirB4, truncation

CENTER ID:	AnphA.17381.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.17381.a.A1.GE30772	Full length( AnphA.17381.a )		1	277

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.445
RefSeq:	YP_505022.1
UniProt:	Q2GKS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEWFKLLATT LSDKLTPDDI LRIDDAIEGN FKLKKEDRKL RNLVPFLGIG GEDTLASRMM MWHSEGSHAA LFDNDEDVLD FTKSRVFGFE MGYLLKDPMA LAPTLLYLFH KISISLDGTP SMIILDEAWA LIDNPVFAPR IKDWLKVLRK LNTFVIFATQ SVEDASKSQI SDTLVQQTAT QIFLPNLKAT SAYRDVFMLT EREYSLIKYT DPGTRFFLVK QGVSAVVARI DLRGLDDTIN VLSGRAETVA ILREIMEEVG DDPNVWLPIF YQRVKNA

NT Sequence

ATGGAGTGGT TTAAGTTATT GGCTACTACT CTGTCAGATA AGCTTACGCC TGACGATATA TTGCGCATTG ATGATGCTAT AGAGGGTAAT TTCAAGCTCA AAAAGGAAGA CAGAAAGTTA CGTAATCTGG TTCCTTTTTT GGGTATAGGT GGAGAAGATA CTCTAGCGAG TCGCATGATG ATGTGGCATT CAGAAGGGTC GCACGCGGCG CTTTTTGATA ATGACGAAGA TGTATTAGAC TTCACTAAGT CTAGAGTATT TGGATTTGAG ATGGGATATT TGCTAAAAGA TCCCATGGCT CTTGCTCCGA CGCTTTTGTA TTTGTTTCAC AAGATAAGTA TTTCTCTCGA TGGAACTCCT TCTATGATTA TCTTGGACGA AGCGTGGGCG CTGATAGATA ATCCTGTTTT TGCGCCTAGG ATTAAAGACT GGCTAAAAGT TCTTAGGAAG CTCAATACTT TTGTCATATT TGCGACACAG AGTGTTGAGG ATGCTAGTAA AAGTCAGATA AGTGATACCT TAGTTCAGCA GACTGCCACT CAGATATTTT TGCCTAATCT TAAGGCTACT AGTGCTTACA GGGATGTTTT CATGCTGACT GAGCGGGAGT ATTCTTTAAT AAAATATACA GATCCTGGCA CTAGGTTTTT CCTAGTCAAG CAGGGGGTCA GTGCTGTTGT GGCCAGGATA GATTTACGAG GGCTCGATGA CACGATAAAC GTTTTGTCAG GTAGGGCCGA GACCGTTGCC ATACTTCGTG AAATTATGGA AGAAGTAGGG GATGATCCAA ATGTGTGGTT GCCTATTTTT TATCAAAGGG TGAAAAATGC A

Details for AnphA.17381.a.A1.GE30772

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMEWFKLLAT TLSDKLTPDD ILRIDDAIEG NFKLKKEDRK LRNLVPFLGI GGEDTLASRM MMWHSEGSHA ALFDNDEDVL DFTKSRVFGF EMGYLLKDPM ALAPTLLYLF HKISISLDGT PSMIILDEAW ALIDNPVFAP RIKDWLKVLR KLNTFVIFAT QSVEDASKSQ ISDTLVQQTA TQIFLPNLKA TSAYRDVFML TEREYSLIKY TDPGTRFFLV KQGVSAVVAR IDLRGLDDTI NVLSGRAETV AILREIMEEV XDDPNVWLPI FYQX

Validated NT Sequence

attttgttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggagt ggtttaagtt attggctact actctgtcag ataagcttac gcctgacgat atattgcgca ttgatgatgc tatagagggt aatttcaagc tcaaaaagga agacagaaag ttacgtaatc tggttccttt tttgggtata ggtggagaag atactctagc gagtcgcatg atgatgtggc attcagaagg gtcgcacgcg gcgctttttg ataatgacga agatgtatta gacttcacta agtctagagt atttggattt gagatgggat atttgctaaa agatcccatg gctcttgctc cgacgctttt gtatttgttt cacaagataa gtatttctct cgatggaact ccttctatga ttatcttgga cgaagcgtgg gcgctgatag ataatcctgt ttttgcgcct aggattaaag actggctaaa agttcttagg aagctcaata cttttgtcat atttgcgaca cagagtgttg aggatgctag taaaagtcag ataagtgata ccttagttca gcagactgcc actcagatat ttttgcctaa tcttaaggct actagtgctt acagggatgt tttcatgctg actgagcggg agtattcttt aataaaatat acagatcctg gcactaggtt tttcctagtc aagcaggggg tcagtgctgt tgtggccagg atagatttac gagggctcga tgacacgata aacgttttgt caggtagggc cgagaccgtt gccatacttc gtgaaattat ggaagaagtn ngggatgatc caaatgtgtg gttgcctatt ttttatcaan ggtgaaaaat gcataataaa cagcacgaac aagttctgca gcnancttct cnagnnnnnc tgctancaaa nccngaaagg aagctgantt ggctgctgcc nnnnnnnaaa nnnnnnnnnn nanaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagtggttt aagttattgg ctactactct gtcagataag cttacgcctg acgatatatt gcgcattgat gatgctatag agggtaattt caagctcaaa aaggaagaca gaaagttacg taatctggtt ccttttttgg gtataggtgg agaagatact ctagcgagtc gcatgatgat gtggcattca gaagggtcgc acgcggcgct ttttgataat gacgaagatg tattagactt cactaagtct agagtatttg gatttgagat gggatatttg ctaaaagatc ccatggctct tgctccgacg cttttgtatt tgtttcacaa gataagtatt tctctcgatg gaactccttc tatgattatc ttggacgaag cgtgggcgct gatagataat cctgtttttg cgcctaggat taaagactgg ctaaaagttc ttaggaagct caatactttt gtcatatttg cgacacagag tgttgaggat gctagtaaaa gtcagataag tgatacctta gttcagcaga ctgccactca gatatttttg cctaatctta aggctactag tgcttacagg gatgttttca tgctgactga gcgggagtat tctttaataa aatatacaga tcctggcact aggtttttcc tagtcaagca gggggtcagt gctgttgtgg ccaggataga tttacgaggg ctcgatgaca cgataaacgt tttgtcaggt agggccgaga ccgttgccat acttcgtgaa attatggaag aagtagggga tgatccaaat gtgtggttgc ctatttttta tcaaagggtg aaaaatgcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa