AnphA.17380.a

Riboflavin biosynthesis protein RibD

CENTER ID: AnphA.17380.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17380.a.A1.GE30771 Full length( AnphA.17380.a ) 1 364
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17380.a.A1.PW30658 Full length( AnphA.17380.a ) 1 364

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.32
RefSeq: YP_504671.1
UniProt: Q2GLT0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVPDERFMAM ALTLARRGLG NVYPNPAVGC VVIRDGVVVG RGWTVAGGRP HAEIVALQNA GNRAIGATVY VTLEPCCHDG VTGPCTSALI NAGVSRVVIG VQDPDYRVSG KGISSLEAAG IEVKYGVLQQ QAEALNAGFF YSKVLHRPLI TAKLATTLDG KISFDEGCDR WITNDLTRKW VHKQRAMYDA ILVGSNTVVA DDPMLDCRLP GLEKHSPIRV VVDRSGKLLA HHKIVCTAGT IPTYIVTDND PQCVFENVKY IKVDGGTEFL ENMVRALTAQ LGITRLFVEG GGILITELLK KKLIDRFIWT RANKISGSKG LNSITDIDAQ FGQCCKFVKT DTLSFFDDTV DLFDVKYTQL GSNL
NT Sequence
ATGGTGCCTG ACGAGAGATT CATGGCTATG GCCTTGACCT TAGCGCGCAG AGGATTGGGT AATGTTTATC CAAACCCTGC GGTAGGATGT GTTGTAATAC GTGATGGTGT GGTTGTAGGC AGAGGGTGGA CTGTTGCGGG AGGTAGACCT CATGCTGAGA TCGTAGCACT ACAAAATGCA GGGAATCGTG CTATAGGAGC TACGGTATAT GTCACACTTG AGCCGTGTTG TCATGATGGT GTAACAGGGC CATGTACTTC GGCATTAATC AATGCTGGGG TTAGTAGAGT TGTTATAGGA GTGCAGGATC CGGATTACAG AGTATCTGGC AAGGGAATTA GTTCTCTAGA AGCTGCTGGT ATAGAGGTTA AATACGGGGT TTTGCAGCAG CAAGCAGAGG CCTTAAATGC TGGTTTTTTC TACAGCAAAG TACTACATAG ACCATTAATT ACGGCTAAGC TGGCAACGAC TCTAGATGGG AAAATATCGT TTGATGAAGG GTGTGATCGC TGGATAACAA ACGATCTTAC CAGAAAATGG GTGCATAAGC AGCGTGCAAT GTATGACGCT ATCTTGGTGG GTAGCAATAC CGTTGTAGCC GATGATCCAA TGCTAGACTG TAGGTTACCG GGGCTGGAAA AGCATTCTCC AATACGGGTA GTCGTTGATA GGTCTGGTAA ATTGTTGGCG CATCATAAAA TAGTTTGTAC AGCAGGTACC ATTCCCACAT ACATTGTTAC GGATAACGAT CCTCAGTGCG TTTTCGAGAA TGTAAAATAT ATTAAAGTTG ATGGAGGAAC AGAATTTTTG GAAAACATGG TACGTGCTCT CACCGCTCAG TTAGGCATTA CTAGGCTGTT TGTTGAGGGA GGGGGTATCT TGATCACGGA GCTGCTAAAA AAAAAGTTGA TAGACCGTTT TATATGGACT AGGGCAAATA AAATAAGTGG CAGTAAAGGA TTGAATTCCA TTACCGACAT TGATGCGCAG TTTGGCCAGT GCTGTAAGTT TGTAAAGACA GATACGCTAT CTTTTTTTGA CGATACAGTG GACCTTTTCG ATGTTAAATA CACTCAATTA GGAAGCAACC TC
Details for AnphA.17380.a.A1.GE30771
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVPDERFMAM ALTLARRGLG NVYPNPAVGC VVIRDGVVVG RGWTVAGGRP HAEIVALQNA GNRAIGATVY VTLEPCCHDG VTGPCTSALI NAGVSRVVIG VQDPDYRVSG KGISSLEAAG IEVKYGVLQQ QAEALNAGFF YSKVLHRPLI TAKLATTLDG KISFDEGCDR WITNDLTRKW VHKQRAMYDA ILVGSNTVVA DDPMLDCRLP GLEKHSPIRV VVDRSGKLLA HHKIVCTAGT IPTYIVTDND PQCVFENVKY IKVDGGTEFL ENMVRALTAQ LGITRLFVEG GGILITELLK KKLIDRFIWT RANKISGSKG LNSITDIDAQ FGQCCKFVKT DTLSFFDDTV DLFDVKYTQL GS
Validated NT Sequence
tgcttcctaa ttgagtgtat ttaacatcga aaaggtccac tgtatcgtca aaaaaagata gcgtatctgt ctttacaaac ttacagcact ggccaaactg cgcatcaatg tcggtaatgg aattcaatcc tttactgcca cttattttat ttgccctagt ccatataaaa cggtctatca actttttttt tagcagctcc gtgatcaaga taccccctcc ctcaacaaac agcctagtaa tgcctaactg agcggtgaga gcacgtacca tgttttccaa aaattctgtt cctccatcaa ctttaatata ttttacattc tcgaaaacgc actgaggatc gttatccgta acaatgtatg tgggaatggt acctgctgta caaactattt tatgatgcgc caacaattta ccagacctat caacgactac ccgtattgga gaatgctttt ccagccccgg taacctacag tctagcattg gatcatcggc tacaacggta ttgctaccca ccaagatagc gtcatacatt gcacgctgct tatgcaccca ttttctggta agatcgtttg ttatccagcg atcacaccct tcatcaaacg atattttccc atctagagtc gttgccagct tagccgtaat taatggtcta tgtagtactt tgctgtagaa aaaaccagca tttaaggcct ctgcttgctg ctgcaaaacc ccgtatttaa cctctatacc agcagcttct agagaactaa ttcccttgcc agatactctg taatccggat cctgcactcc tataacaact ctactaaccc cagcattgat taatgccgaa gtacatggcc ctgttacacc atcatgacaa cacggctcaa gtgtgacata taccgtagct cctatagcac gattccctgc attttgtagt gctacgatct cagcatgagg tctacctccc gcaacagtcc accctctgcc tacaaccaca ccatcacgta ttacaacaca tcctaccgca gggtttggat aaacattacc caatcctctg cgcgctaagg tcaaggccat agccatgaat ctctcgtcag gcaccatcga accaggaccc tgggtctgag cttccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPDERFMA MALTLARRGL GNVYPNPAVG CVVIRDGVVV GRGWTVAGGR PHAEIVALQN AGNRAIGATV YVTLEPCCHD GVTGPCTSAL INAGVSRVVI GVQDPDYRVS GKGISSLEAA GIEVKYGVLQ QQAEALNAGF FYSKVLHRPL ITAKLATTLD GKISFDEGCD RWITNDLTRK WVHKQRAMYD AILVGSNTVV ADDPMLDCRL PGLEKHSPIR VVVDRSGKLL AHHKIVCTAG TIPTYIVTDN DPQCVFENVK YIKVDGGTEF LENMVRALTA QLGITRLFVE GGGILITELL KKKLIDRFIW TRANKISGSK GLNSITDIDA QFGQCCKFVK TDTLSFFDDT VDLFDVKYTQ LGSNL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcctgac gagagattca tggctatggc cttgacctta gcgcgcagag gattgggtaa tgtttatcca aaccctgcgg taggatgtgt tgtaatacgt gatggtgtgg ttgtaggcag agggtggact gttgcgggag gtagacctca tgctgagatc gtagcactac aaaatgcagg gaatcgtgct ataggagcta cggtatatgt cacacttgag ccgtgttgtc atgatggtgt aacagggcca tgtacttcgg cattaatcaa tgctggggtt agtagagttg ttataggagt gcaggatccg gattacagag tatctggcaa gggaattagt tctctagaag ctgctggtat agaggttaaa tacggggttt tgcagcagca agcagaggcc ttaaatgctg gttttttcta cagcaaagta ctacatagac cattaattac ggctaagctg gcaacgactc tagatgggaa aatatcgttt gatgaagggt gtgatcgctg gataacaaac gatcttacca gaaaatgggt gcataagcag cgtgcaatgt atgacgctat cttggtgggt agcaataccg ttgtagccga tgatccaatg ctagactgta ggttaccggg gctggaaaag cattctccaa tacgggtagt cgttgatagg tctggtaaat tgttggcgca tcataaaata gtttgtacag caggtaccat tcccacatac attgttacgg ataacgatcc tcagtgcgtt ttcgagaatg taaaatatat taaagttgat ggaggaacag aatttttgga aaacatggta cgtgctctca ccgctcagtt aggcattact aggctgtttg ttgagggagg gggtatcttg atcacggagc tgctaaaaaa aaagttgata gaccgtttta tatggactag ggcaaataaa ataagtggca gtaaaggatt gaattccatt accgacattg atgcgcagtt tggccagtgc tgtaagtttg taaagacaga tacgctatct ttttttgacg atacagtgga ccttttcgat gttaaataca ctcaattagg aagcaacctc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for AnphA.17380.a.A1.PW30658
PURIFICATION DATe: 2/11/2011
CONCENTRATION: 30.61mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MVPDERFMAM ALTLARRGLG NVYPNPAVGC VVIRDGVVVG RGWTVAGGRP HAEIVALQNA GNRAIGATVY VTLEPCCHDG VTGPCTSALI NAGVSRVVIG VQDPDYRVSG KGISSLEAAG IEVKYGVLQQ QAEALNAGFF YSKVLHRPLI TAKLATTLDG KISFDEGCDR WITNDLTRKW VHKQRAMYDA ILVGSNTVVA DDPMLDCRLP GLEKHSPIRV VVDRSGKLLA HHKIVCTAGT IPTYIVTDND PQCVFENVKY IKVDGGTEFL ENMVRALTAQ LGITRLFVEG GGILITELLK KKLIDRFIWT RANKISGSKG LNSITDIDAQ FGQCCKFVKT DTLSFFDDTV DLFDVKYTQL GS
Validated NT Sequence
tgcttcctaa ttgagtgtat ttaacatcga aaaggtccac tgtatcgtca aaaaaagata gcgtatctgt ctttacaaac ttacagcact ggccaaactg cgcatcaatg tcggtaatgg aattcaatcc tttactgcca cttattttat ttgccctagt ccatataaaa cggtctatca actttttttt tagcagctcc gtgatcaaga taccccctcc ctcaacaaac agcctagtaa tgcctaactg agcggtgaga gcacgtacca tgttttccaa aaattctgtt cctccatcaa ctttaatata ttttacattc tcgaaaacgc actgaggatc gttatccgta acaatgtatg tgggaatggt acctgctgta caaactattt tatgatgcgc caacaattta ccagacctat caacgactac ccgtattgga gaatgctttt ccagccccgg taacctacag tctagcattg gatcatcggc tacaacggta ttgctaccca ccaagatagc gtcatacatt gcacgctgct tatgcaccca ttttctggta agatcgtttg ttatccagcg atcacaccct tcatcaaacg atattttccc atctagagtc gttgccagct tagccgtaat taatggtcta tgtagtactt tgctgtagaa aaaaccagca tttaaggcct ctgcttgctg ctgcaaaacc ccgtatttaa cctctatacc agcagcttct agagaactaa ttcccttgcc agatactctg taatccggat cctgcactcc tataacaact ctactaaccc cagcattgat taatgccgaa gtacatggcc ctgttacacc atcatgacaa cacggctcaa gtgtgacata taccgtagct cctatagcac gattccctgc attttgtagt gctacgatct cagcatgagg tctacctccc gcaacagtcc accctctgcc tacaaccaca ccatcacgta ttacaacaca tcctaccgca gggtttggat aaacattacc caatcctctg cgcgctaagg tcaaggccat agccatgaat ctctcgtcag gcaccatcga accaggaccc tgggtctgag cttccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPDERFMA MALTLARRGL GNVYPNPAVG CVVIRDGVVV GRGWTVAGGR PHAEIVALQN AGNRAIGATV YVTLEPCCHD GVTGPCTSAL INAGVSRVVI GVQDPDYRVS GKGISSLEAA GIEVKYGVLQ QQAEALNAGF FYSKVLHRPL ITAKLATTLD GKISFDEGCD RWITNDLTRK WVHKQRAMYD AILVGSNTVV ADDPMLDCRL PGLEKHSPIR VVVDRSGKLL AHHKIVCTAG TIPTYIVTDN DPQCVFENVK YIKVDGGTEF LENMVRALTA QLGITRLFVE GGGILITELL KKKLIDRFIW TRANKISGSK GLNSITDIDA QFGQCCKFVK TDTLSFFDDT VDLFDVKYTQ LGSNL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCTGAC GAGAGATTCA TGGCTATGGC CTTGACCTTA GCGCGCAGAG GATTGGGTAA TGTTTATCCA AACCCTGCGG TAGGATGTGT TGTAATACGT GATGGTGTGG TTGTAGGCAG AGGGTGGACT GTTGCGGGAG GTAGACCTCA TGCTGAGATC GTAGCACTAC AAAATGCAGG GAATCGTGCT ATAGGAGCTA CGGTATATGT CACACTTGAG CCGTGTTGTC ATGATGGTGT AACAGGGCCA TGTACTTCGG CATTAATCAA TGCTGGGGTT AGTAGAGTTG TTATAGGAGT GCAGGATCCG GATTACAGAG TATCTGGCAA GGGAATTAGT TCTCTAGAAG CTGCTGGTAT AGAGGTTAAA TACGGGGTTT TGCAGCAGCA AGCAGAGGCC TTAAATGCTG GTTTTTTCTA CAGCAAAGTA CTACATAGAC CATTAATTAC GGCTAAGCTG GCAACGACTC TAGATGGGAA AATATCGTTT GATGAAGGGT GTGATCGCTG GATAACAAAC GATCTTACCA GAAAATGGGT GCATAAGCAG CGTGCAATGT ATGACGCTAT CTTGGTGGGT AGCAATACCG TTGTAGCCGA TGATCCAATG CTAGACTGTA GGTTACCGGG GCTGGAAAAG CATTCTCCAA TACGGGTAGT CGTTGATAGG TCTGGTAAAT TGTTGGCGCA TCATAAAATA GTTTGTACAG CAGGTACCAT TCCCACATAC ATTGTTACGG ATAACGATCC TCAGTGCGTT TTCGAGAATG TAAAATATAT TAAAGTTGAT GGAGGAACAG AATTTTTGGA AAACATGGTA CGTGCTCTCA CCGCTCAGTT AGGCATTACT AGGCTGTTTG TTGAGGGAGG GGGTATCTTG ATCACGGAGC TGCTAAAAAA AAAGTTGATA GACCGTTTTA TATGGACTAG GGCAAATAAA ATAAGTGGCA GTAAAGGATT GAATTCCATT ACCGACATTG ATGCGCAGTT TGGCCAGTGC TGTAAGTTTG TAAAGACAGA TACGCTATCT TTTTTTGACG ATACAGTGGA CCTTTTCGAT GTTAAATACA CTCAATTAGG AAGCAACCTC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA