AnphA.17322.a

PmbA protein

CENTER ID: AnphA.17322.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17322.a.A1.GE30770 Full length( AnphA.17322.a ) 1 443
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.758
RefSeq: YP_505286.1
UniProt: Q2GK15

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSALNIAEDI IKLIKSRGVE GEVVLTQHET TSVTQRMMRL EEMIASSGCN VGIRVIVGKK YSCISSNDLE RSEELVDAAV KMADLSPEDP FISVSDQQGT YTNTASDLMI FDDSIVEMDS IRDILQEMEG EALSVDSRIV NSEGASFSKS LTETALMTTR GFCGSYKKTY FSSSISVVAA SDDEKMEVDY SFAVKSKFSD LEKSRVLGRE AANRALRKLN ARELQTCKMP VLFENRVASS LLSNFASAIN GASIADKTSF LLNDLGTNVF GSDIYIVDDP LMVSGISSRP FDGEGILSTK RSIVERGVLK SWILDMRTAK QLALSTTGHA VRKDNASVSP GVSNFYIQRS DITVQELMSD IKRGLYITDL FGFGINFATG DYSQGAFGYM IENGDITYPV HGITIAGNLR DMFSSAQVAN DLIFLRSVNS PTLRFGDMVV SGA
NT Sequence
TTGAGTGCCC TGAATATAGC AGAAGATATA ATTAAACTTA TAAAGTCTCG GGGAGTTGAG GGAGAAGTCG TATTAACTCA GCACGAGACT ACCTCTGTTA CCCAGCGTAT GATGAGGCTC GAGGAAATGA TTGCGAGCAG CGGATGTAAT GTTGGGATAC GCGTGATTGT GGGTAAGAAG TATTCATGTA TTTCATCGAA TGATTTAGAG AGGTCTGAAG AGCTAGTAGA TGCTGCTGTA AAGATGGCAG ACTTATCTCC TGAGGATCCA TTTATCTCTG TAAGTGATCA ACAAGGAACG TATACCAATA CTGCTTCTGA TCTTATGATC TTCGATGATT CTATTGTTGA GATGGATTCT ATACGTGACA TTCTTCAGGA AATGGAGGGT GAAGCCCTGT CGGTGGATTC TAGAATAGTA AATTCTGAAG GAGCAAGTTT CTCGAAGTCG TTGACGGAGA CTGCCCTTAT GACAACAAGA GGGTTTTGTG GTTCATATAA GAAAACATAT TTCTCTTCAT CTATATCTGT AGTAGCGGCA TCTGATGATG AGAAGATGGA GGTAGACTAT AGCTTTGCGG TGAAGAGTAA ATTCAGTGAT TTAGAGAAGT CGAGGGTTTT AGGGAGAGAA GCTGCTAATC GGGCTCTGCG GAAGCTGAAT GCTCGCGAGC TTCAAACATG TAAAATGCCA GTTTTATTTG AAAATAGGGT GGCATCTTCA TTGTTGTCCA ATTTTGCTTC AGCTATTAAT GGAGCTTCTA TCGCTGATAA GACGTCTTTT CTTCTAAATG ATTTAGGAAC TAATGTTTTT GGCAGTGATA TTTATATAGT GGATGATCCT CTCATGGTAT CTGGTATATC ATCGCGTCCC TTTGATGGTG AGGGTATACT GTCTACAAAG CGGAGCATAG TAGAAAGAGG TGTTCTCAAG AGTTGGATTT TAGATATGAG AACGGCAAAA CAACTGGCAT TGTCCACGAC AGGTCATGCG GTAAGGAAAG ATAATGCGTC TGTTTCTCCC GGTGTAAGTA ATTTTTACAT ACAGAGATCG GATATTACAG TACAGGAATT AATGTCAGAT ATTAAGAGAG GGTTGTACAT CACTGATCTT TTCGGATTTG GAATAAATTT TGCTACAGGA GATTATAGTC AAGGAGCTTT TGGTTATATG ATCGAAAATG GAGATATAAC CTATCCAGTC CATGGAATTA CTATAGCAGG GAATCTACGA GATATGTTTT CAAGCGCACA AGTTGCGAAC GATTTAATTT TTCTCCGATC AGTAAACTCT CCGACATTAA GGTTCGGTGA TATGGTTGTT TCTGGTGCT
Details for AnphA.17322.a.A1.GE30770
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 70
Validated AA Sequence
MGTLXXXTXG PGSMLSALNI AXXIIKLIKX RGXXXEVXLX XHETTSVTXR MMRLEEMIAS XGCNVGIXVI VGXXYSXISS NDLXRXEXXV DAAVKMADLS PEDPFISVSD QQGTYTNTAS DLMIFDDSIV EMDSIRDILQ EMEGEALSVD SRIVNSEGAS FSKSLTETAL MTTRGFCGSY KKTYFSSSIS VVAASDDEKM EVDYSFAVKS KFXDLXKSRV LGREAANRAL RKLNARELQT CKMPVLFENR VXSSLLSNFA SAINGASIAD KTSFXLXDXX TNXFGSDXYI VXDPXMXSGX XSXPFDGXGX TXYXXXXXXX XXXXXXX
Validated NT Sequence
antttgttta ctttaanaag gaganatacc ntggctcatc accatcacca tcatatgggt accctgnnnn ctcanacccn gggtcctggn tcgatgttga gtgccctgaa tatagcnnan natataatta aacttataaa gncncgggga gntgangnag aagtcntatt anctcnncac gagactacct ctgttacccn ncgnatgatg aggctcgagg aaatgattgc nagcnncgga tgtaatgttg ggatacncgt gattgtgggt aanaantatt catgnatttc atcgaatgat ttanagaggn ctgaanannn ngtagatgct gctgtaaaga tggcagactt atctcctgag gatccattta tctctgtaag tgatcaacaa ggaacgtata ccaatactgc ttctgatctt atgatcttcg atgattctat tgttgagatg gattctatac gtgacattct tcaggaaatg gagggtgaag ccctgtcggt ggattctaga atagtaaatt ctgaaggagc aagtttctcg aagtcgttga cggagactgc ccttatgaca acaagagggt tttgtggttc atataagaaa acatatttct cttcatctat atctgtagta gcggcatctg atgatgagaa gatggaggta gactatagct ttgcggtgaa gagtaaattc antgatttan agaagtcgag ggttttaggg agagaagctg ctaatcgggc tctgcggaag ctgaatgctc gcgagcttca aacatgtaaa atgccagttt tatttgaaaa tagggtgnca tcttcattgt tgtccaattt tgcttcagct attaatggag cttctatcgc tgataagacg tcttttcntc taantgattt nnnnactaat gnntttggca gtgatnttta tatagtgnan gatcctcnca tgnnatctgg nannncatcg cnnccctttg atggnngagg gnatactgnc tacaannnnn nnnnnnnnaa ngannngntn nnnngnnntn gnntttagnn nnnnanaann gnaannannn nnntnnccnc nannggncan nnngnnnnnn nnnnnangng tcnnttnnnn nnnnnnnnnn anttttnnnn nnannnnnnn nnngnnnnnn nnnnnnnnnn nnnnnnnnng ntnnnnnnnn ntnngnnttn nnnnnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSALNIAE DIIKLIKSRG VEGEVVLTQH ETTSVTQRMM RLEEMIASSG CNVGIRVIVG KKYSCISSND LERSEELVDA AVKMADLSPE DPFISVSDQQ GTYTNTASDL MIFDDSIVEM DSIRDILQEM EGEALSVDSR IVNSEGASFS KSLTETALMT TRGFCGSYKK TYFSSSISVV AASDDEKMEV DYSFAVKSKF SDLEKSRVLG REAANRALRK LNARELQTCK MPVLFENRVA SSLLSNFASA INGASIADKT SFLLNDLGTN VFGSDIYIVD DPLMVSGISS RPFDGEGILS TKRSIVERGV LKSWILDMRT AKQLALSTTG HAVRKDNASV SPGVSNFYIQ RSDITVQELM SDIKRGLYIT DLFGFGINFA TGDYSQGAFG YMIENGDITY PVHGITIAGN LRDMFSSAQV ANDLIFLRSV NSPTLRFGDM VVSGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgagtgcc ctgaatatag cagaagatat aattaaactt ataaagtctc ggggagttga gggagaagtc gtattaactc agcacgagac tacctctgtt acccagcgta tgatgaggct cgaggaaatg attgcgagca gcggatgtaa tgttgggata cgcgtgattg tgggtaagaa gtattcatgt atttcatcga atgatttaga gaggtctgaa gagctagtag atgctgctgt aaagatggca gacttatctc ctgaggatcc atttatctct gtaagtgatc aacaaggaac gtataccaat actgcttctg atcttatgat cttcgatgat tctattgttg agatggattc tatacgtgac attcttcagg aaatggaggg tgaagccctg tcggtggatt ctagaatagt aaattctgaa ggagcaagtt tctcgaagtc gttgacggag actgccctta tgacaacaag agggttttgt ggttcatata agaaaacata tttctcttca tctatatctg tagtagcggc atctgatgat gagaagatgg aggtagacta tagctttgcg gtgaagagta aattcagtga tttagagaag tcgagggttt tagggagaga agctgctaat cgggctctgc ggaagctgaa tgctcgcgag cttcaaacat gtaaaatgcc agttttattt gaaaataggg tggcatcttc attgttgtcc aattttgctt cagctattaa tggagcttct atcgctgata agacgtcttt tcttctaaat gatttaggaa ctaatgtttt tggcagtgat atttatatag tggatgatcc tctcatggta tctggtatat catcgcgtcc ctttgatggt gagggtatac tgtctacaaa gcggagcata gtagaaagag gtgttctcaa gagttggatt ttagatatga gaacggcaaa acaactggca ttgtccacga caggtcatgc ggtaaggaaa gataatgcgt ctgtttctcc cggtgtaagt aatttttaca tacagagatc ggatattaca gtacaggaat taatgtcaga tattaagaga gggttgtaca tcactgatct tttcggattt ggaataaatt ttgctacagg agattatagt caaggagctt ttggttatat gatcgaaaat ggagatataa cctatccagt ccatggaatt actatagcag ggaatctacg agatatgttt tcaagcgcac aagttgcgaa cgatttaatt tttctccgat cagtaaactc tccgacatta aggttcggtg atatggttgt ttctggtgct taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa