AnphA.17300.a

Outer membrane efflux protein

CENTER ID: AnphA.17300.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17300.a.A2.GE30766 Mature protein( AnphA.17300.a ) 39 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1187
RefSeq: YP_505658.1
UniProt: Q2GIZ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILHTINDWK YTVNWRLPMR KALLLFTLVL MKISSGAYGT SLEEALDKTL NNNLAIKANM YKTKAKKREV VNSAIANILP QVLYELKIQK HGSHQSSYSL ISLTQPVFNG TAIFSIEKAK HLYAIQDITF SMEQQKTLLG TIKTYMSALS AYEVDKLNEH NLKVMQEHLL SAEKRFSVGE ITKTELMQAK AKLAQAKSEA MSAKGRLRAV EASYTHMVGE QPIDLQYPKR PLQIPQSLEE AFDIAKSNNL YLRLSSNLYQ VSKRDVEIAI AKKVLPSLHV TATETFPGND VGRTIHRLEF TLRLPIFEHG VGLIDIDRTN QERQHNLFSM HEAVKKMEAS IISAWEDLIT SRFVLQSASE TVKYTEVALE AITQEANLNL KTTLDVLDIE QELLKAKVNL VNSQSNLIIN QYNLLALIGQ LSID
NT Sequence
TTGATTTTAC ATACCATTAA CGATTGGAAG TACACAGTTA ATTGGCGGTT GCCTATGCGC AAAGCATTAT TACTTTTCAC ACTCGTACTA ATGAAAATAT CATCGGGAGC TTATGGTACA TCACTTGAGG AAGCACTGGA TAAGACTCTC AACAATAACT TGGCTATCAA AGCCAATATG TACAAGACGA AGGCTAAAAA ACGTGAGGTA GTTAATTCTG CAATAGCAAA CATTCTACCA CAAGTTTTAT ACGAACTTAA AATACAAAAA CATGGCTCTC ATCAATCAAG CTATTCCTTG ATATCCCTTA CCCAGCCGGT GTTCAACGGG ACCGCAATAT TTAGCATTGA AAAAGCAAAG CACCTATACG CCATCCAGGA TATCACATTC TCTATGGAAC AACAGAAAAC TCTTCTAGGT ACCATAAAAA CATATATGAG TGCTTTATCA GCCTACGAAG TAGATAAACT GAATGAGCAT AACCTAAAGG TGATGCAGGA GCATTTGCTG TCAGCCGAAA AACGCTTTTC TGTTGGTGAG ATTACAAAAA CTGAGCTAAT GCAGGCAAAA GCAAAGCTGG CACAAGCAAA ATCCGAAGCC ATGAGCGCAA AAGGTAGACT GAGAGCTGTG GAGGCCAGTT ATACTCACAT GGTGGGGGAA CAACCTATAG ATCTACAATA TCCTAAGCGC CCTCTACAAA TCCCGCAATC TCTCGAAGAA GCTTTTGATA TCGCTAAAAG CAATAATCTA TATCTACGAT TGTCTAGCAA CCTCTACCAA GTCTCAAAAC GTGATGTGGA AATTGCTATT GCTAAAAAGG TTTTGCCCTC TTTGCATGTA ACAGCTACTG AAACATTTCC AGGTAACGAT GTAGGACGTA CAATTCACCG GCTAGAGTTC ACGTTGCGTC TGCCCATTTT CGAGCATGGT GTTGGGTTGA TAGATATAGA CCGCACTAAT CAGGAGCGTC AACACAATCT CTTCTCTATG CATGAAGCAG TCAAAAAGAT GGAAGCATCT ATTATAAGCG CTTGGGAAGA TCTGATAACA TCAAGGTTTG TACTCCAATC TGCAAGCGAA ACTGTAAAGT ACACAGAAGT TGCTTTAGAA GCCATAACAC AAGAAGCAAA CTTAAACTTA AAGACTACCT TGGATGTATT GGATATTGAG CAGGAACTTC TGAAAGCAAA AGTAAACCTA GTTAATTCAC AGAGCAATTT AATTATAAAT CAGTACAATT TATTGGCTTT AATCGGACAG TTGAGCATTG AT
Details for AnphA.17300.a.A2.GE30766
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 80
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGTSLEEAL DKTLNNNLAI KANMYKTKAK KREVVNSAIA NILPQVLYEL KIQKHGSHQS SYSLISLTQP VFNGTAIFSI EKAKHLYAIQ DITFSMEQQK TLLGTIKTYM SALSAYEVDK LNEHNLKVMQ EHLLSAEKRV SVGEITKTEL MQAKAKLAQA KSEAMSAKGR LRAVEASYTH MVGEQPIDLQ YPKRPLQIPQ SLEEAFDIAK SNNLYLRLSS NLYQVSKRDV EIAIAKKVLP SLHVTATETF PGNDVXRTIH RLEFTLRLXI FXHGVGLIDI DRTNXXRQXX LFSMHEAVKX XXXIYYXRLX XLXXXXXXXX LXXXCKXXXX XXXXXXXKXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggta catcacttga ggaagcactg gataagactc tcaacaataa cttggctatc aaagccaata tgtacaagac gaaggctaaa aaacgtgagg tagttaattc tgcaatagca aacattctac cacaagtttt atacgaactt aaaatacaaa aacatggctc tcatcaatca agctattcct tgatatccct tacccagccg gtgttcaacg ggaccgcaat atttagcatt gaaaaagcaa agcacctata cgccatccag gatatcacat tctctatgga acaacagaaa actcttctag gtaccataaa aacatatatg agtgctttat cagcctacga agtagataaa ctgaatgagc ataacctaaa ggtgatgcag gagcatttgc tgtcagccga aaaacgcgtt tctgttggtg agattacaaa aactgagcta atgcaggcaa aagcaaagct ggcacaagca aaatccgaag ccatgagcgc aaaaggtaga ctgagagctg tggaggccag ttatactcac atggtggggg aacaacctat agatctacaa tatcctaagc gccctctaca aatcccgcaa tctctcgaag aagcttttga tatcgctaaa agcaataatc tatatctacg attgtctagc aacctctacc aagtctcaaa acgtgatgtg gaaattgcta ttgctaaaaa ggttttgccc tctttgcatg taacagctac tgaaacattt ccaggtaacg atgtangacg tacaattcac cggctagagt tcacgttgcg tctgnccatt ttcnagcatg gtgttgggtt gatagatata gaccgcacta atcnnnagcg tcaacannat ctcttctcta tgcatgaagc agtcaaaaan ntngnannca tctattatna ncgcttggna nnnctganan atcnagnttn gnacnncnnt ctgnnncnaa antgtaaann acncnnnant ntttnnnnnc nnannnnnnn naaannnnnn nnnnnctncn nnnnnnnngn nntnnnnnnn nnntnnnnnn nnnnnnnnnn nnaannnann nnnnnnnnta nnnnnnnnna nnnnnnnnnn nt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGTSLEEAL DKTLNNNLAI KANMYKTKAK KREVVNSAIA NILPQVLYEL KIQKHGSHQS SYSLISLTQP VFNGTAIFSI EKAKHLYAIQ DITFSMEQQK TLLGTIKTYM SALSAYEVDK LNEHNLKVMQ EHLLSAEKRF SVGEITKTEL MQAKAKLAQA KSEAMSAKGR LRAVEASYTH MVGEQPIDLQ YPKRPLQIPQ SLEEAFDIAK SNNLYLRLSS NLYQVSKRDV EIAIAKKVLP SLHVTATETF PGNDVGRTIH RLEFTLRLPI FEHGVGLIDI DRTNQERQHN LFSMHEAVKK MEASIISAWE DLITSRFVLQ SASETVKYTE VALEAITQEA NLNLKTTLDV LDIEQELLKA KVNLVNSQSN LIINQYNLLA LIGQLSID
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtacatca cttgaggaag cactggataa gactctcaac aataacttgg ctatcaaagc caatatgtac aagacgaagg ctaaaaaacg tgaggtagtt aattctgcaa tagcaaacat tctaccacaa gttttatacg aacttaaaat acaaaaacat ggctctcatc aatcaagcta ttccttgata tcccttaccc agccggtgtt caacgggacc gcaatattta gcattgaaaa agcaaagcac ctatacgcca tccaggatat cacattctct atggaacaac agaaaactct tctaggtacc ataaaaacat atatgagtgc tttatcagcc tacgaagtag ataaactgaa tgagcataac ctaaaggtga tgcaggagca tttgctgtca gccgaaaaac gcttttctgt tggtgagatt acaaaaactg agctaatgca ggcaaaagca aagctggcac aagcaaaatc cgaagccatg agcgcaaaag gtagactgag agctgtggag gccagttata ctcacatggt gggggaacaa cctatagatc tacaatatcc taagcgccct ctacaaatcc cgcaatctct cgaagaagct tttgatatcg ctaaaagcaa taatctatat ctacgattgt ctagcaacct ctaccaagtc tcaaaacgtg atgtggaaat tgctattgct aaaaaggttt tgccctcttt gcatgtaaca gctactgaaa catttccagg taacgatgta ggacgtacaa ttcaccggct agagttcacg ttgcgtctgc ccattttcga gcatggtgtt gggttgatag atatagaccg cactaatcag gagcgtcaac acaatctctt ctctatgcat gaagcagtca aaaagatgga agcatctatt ataagcgctt gggaagatct gataacatca aggtttgtac tccaatctgc aagcgaaact gtaaagtaca cagaagttgc tttagaagcc ataacacaag aagcaaactt aaacttaaag actaccttgg atgtattgga tattgagcag gaacttctga aagcaaaagt aaacctagtt aattcacaga gcaatttaat tataaatcag tacaatttat tggctttaat cggacagttg agcattgatt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa