SSGCID: Target Page

AnphA.17299.a

TolB family protein

CENTER ID:	AnphA.17299.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.17299.a.A2.GE30765	Mature protein( AnphA.17299.a )		23	435

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.17299.a.A2.PS01013	Mature protein( AnphA.17299.a )		23	435

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1112
RefSeq:	YP_505595.1
UniProt:	Q2GJ56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIRLGVATF FIFLLFCCGG AVASLRLSIT KGNTGKLSIA FAPLHSFESN LAQKIGESCT DIILKDLNNT GLFDARIEIL DPTSDGAAYS NVWRELKHDI LLTGNVQELS HGRMGIKLFV WDVAAEKKLS GKSFNFEIGG WRRAAHTVAD HIYTRITGNG GYFDSKIAYI ADMLGERRIA LMDYDGANSV YASIDNNGWL SFPRFSPDTK SIVYTLTSQS SPSGRVMLYD MRREVSSLLI GMSGIVSSVR FSPSGREILL SESLNGSTDI YSFSLISKKL TRLTADEFMN TSASYSPDEK SIVFASDRDG GSHLYIMDAN GSNPRKISSG SGRYMSPVWS PNGTWIAFTK VKGARCYIGI MRPNGSKERM IASDCFAEGP SWAPNGQAVL YTKHVQRVEH LATELAMVDL SSMNRRAINT PSDALIAHWS NTMQN

NT Sequence

ATGTCAATTA GGCTTGGAGT TGCTACGTTT TTCATATTTT TATTATTCTG TTGTGGGGGT GCTGTTGCCT CTTTGCGTCT AAGTATCACT AAGGGCAACA CTGGGAAACT AAGTATAGCT TTTGCGCCCT TGCACTCTTT TGAGAGCAAT TTAGCGCAGA AGATTGGCGA GAGTTGCACA GATATCATCT TAAAGGACTT GAACAACACA GGGTTATTTG ATGCGCGTAT TGAGATTCTT GATCCAACCT CAGATGGCGC AGCTTACTCC AATGTATGGA GAGAATTGAA GCATGACATC CTGCTTACAG GCAATGTGCA GGAGCTTTCA CACGGAAGAA TGGGTATCAA GTTATTCGTG TGGGATGTAG CTGCGGAAAA GAAATTGTCT GGCAAGTCTT TTAACTTTGA AATAGGAGGG TGGCGTCGGG CTGCTCATAC CGTTGCAGAT CATATTTATA CCAGGATTAC TGGCAATGGC GGGTACTTCG ACTCAAAAAT TGCGTACATT GCGGATATGC TGGGTGAGAG AAGAATAGCT CTGATGGATT ATGATGGAGC TAACAGTGTT TACGCTTCTA TAGATAATAA TGGATGGCTA TCTTTCCCTA GATTTTCTCC TGATACAAAA AGCATAGTGT ACACTCTCAC ATCGCAGTCT TCTCCTTCTG GGAGAGTTAT GTTGTACGAT ATGAGGAGAG AGGTTTCTTC GCTGCTAATA GGCATGTCTG GTATTGTGTC GTCGGTGAGA TTTTCGCCAA GTGGCCGCGA AATATTGCTA TCAGAATCGC TTAATGGCAG CACTGACATA TACTCCTTTA GTCTTATAAG CAAAAAACTT ACGAGGCTGA CCGCTGATGA GTTCATGAAC ACGTCTGCTT CTTATTCCCC TGATGAGAAA TCTATAGTAT TTGCTTCGGA CCGAGACGGT GGATCTCATC TCTATATTAT GGATGCTAAT GGAAGTAACC CACGCAAAAT AAGCTCAGGC TCAGGCAGGT ACATGTCTCC TGTATGGTCT CCCAACGGAA CTTGGATAGC ATTTACTAAG GTCAAGGGTG CTCGTTGTTA TATTGGGATC ATGCGTCCTA ATGGTAGTAA GGAAAGAATG ATAGCGTCGG ATTGCTTTGC TGAAGGACCG TCTTGGGCGC CGAATGGTCA GGCAGTTTTA TATACTAAAC ATGTTCAAAG AGTGGAGCAT TTGGCCACAG AACTTGCTAT GGTAGACTTA TCAAGCATGA ATAGGAGGGC TATAAATACT CCAAGTGACG CCTTGATCGC ACATTGGTCG AATACAATGC AAAAT

Details for AnphA.17299.a.A2.GE30765

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	94

Validated AA Sequence

MASLRLSITX GNXGKLXIAF APLHSFESNL XXKIGESCTD IILKDLNNTG LFDARIEILD PTSDGAAYSN VWRELKHDIL LTGNVQELSH GRMGIKLFVW DVAAEKKLSG KSFNFEIGGW RRAAHTVADH IYTRITGNGG YFDSKIAYIA DMLGERRIAL MDYDGANSVY ASIDNNGWLS FPRFSPDTKS IVYTLTSQSS PSGRVMLYDM RREVSSLLIG MSGIVSSVRF SPSGREILLS ESLNGSTDIY SFSLISKKLT RLTADEFMNT SASYSPDEKS IVFASDRDGG SHLYIMDANG SNPRKISSGS GRYMSPVWSP NGTWIAFTKV KGARCYIGIM RPNGSKERMI ASDCFAEGPS WAPNGQAVLY TKHVQRVEHL ATELAMVDLS SMNRRAINTP SDALIAHWSN T

Validated NT Sequence

cattgtattc gaccaatgtg cgatcaaggc gtcacttgga gtatttatag ccctcctatt catgcttgat aagtctacca tagcaagttc tgtggccaaa tgctccactc tttgaacatg tttagtatat aaaactgcct gaccattcgg cgcccaagac ggtccttcag caaagcaatc cgacgctatc attctttcct tactaccatt aggacgcatg atcccaatat aacaacgagc acccttgacc ttagtaaatg ctatccaagt tccgttggga gaccatacag gagacatgta cctgcctgag cctgagctta ttttgcgtgg gttacttcca ttagcatcca taatatagag atgagatcca ccgtctcggt ccgaagcaaa tactatagat ttctcatcag gggaataaga agcagacgtg ttcatgaact catcagcggt cagcctcgta agttttttgc ttataagact aaaggagtat atgtcagtgc tgccattaag cgattctgat agcaatattt cgcggccact tggcgaaaat ctcaccgacg acacaatacc agacatgcct attagcagcg aagaaacctc tctcctcata tcgtacaaca taactctccc agaaggagaa gactgcgatg tgagagtgta cactatgctt tttgtatcag gagaaaatct agggaaagat agccatccat tattatctat agaagcgtaa acactgttag ctccatcata atccatcaga gctattcttc tctcacccag catatccgca atgtacgcaa tttttgagtc gaagtacccg ccattgccag taatcctggt ataaatatga tctgcaacgg tatgagcagc ccgacgccac cctcctattt caaagttaaa agacttgcca gacaatttct tttccgcagc tacatcccac acgaataact tgatacccat tcttccgtgt gaaagctcct gcacattgcc tgtaagcagg atgtcatgct tcaattctct ccatacattg gagtaagctg cgccatctga ggttggatca agaatctcaa tacgcgcatc aaataaccct gtgttgttca agtcctttaa gatgatatct gtgcaactct cgccaatctt ntgcgntaaa ttgctctcaa aagagtgcaa gggcgcaaaa gctatantta gtttcccagn gttgccntta gtgatactta gacgcaaaga ggccatcgaa ccaggncccn gggtctgagc ttccaggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASLRLSIT KGNTGKLSIA FAPLHSFESN LAQKIGESCT DIILKDLNNT GLFDARIEIL DPTSDGAAYS NVWRELKHDI LLTGNVQELS HGRMGIKLFV WDVAAEKKLS GKSFNFEIGG WRRAAHTVAD HIYTRITGNG GYFDSKIAYI ADMLGERRIA LMDYDGANSV YASIDNNGWL SFPRFSPDTK SIVYTLTSQS SPSGRVMLYD MRREVSSLLI GMSGIVSSVR FSPSGREILL SESLNGSTDI YSFSLISKKL TRLTADEFMN TSASYSPDEK SIVFASDRDG GSHLYIMDAN GSNPRKISSG SGRYMSPVWS PNGTWIAFTK VKGARCYIGI MRPNGSKERM IASDCFAEGP SWAPNGQAVL YTKHVQRVEH LATELAMVDL SSMNRRAINT PSDALIAHWS NTMQN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcctctttg cgtctaagta tcactaaggg caacactggg aaactaagta tagcttttgc gcccttgcac tcttttgaga gcaatttagc gcagaagatt ggcgagagtt gcacagatat catcttaaag gacttgaaca acacagggtt atttgatgcg cgtattgaga ttcttgatcc aacctcagat ggcgcagctt actccaatgt atggagagaa ttgaagcatg acatcctgct tacaggcaat gtgcaggagc tttcacacgg aagaatgggt atcaagttat tcgtgtggga tgtagctgcg gaaaagaaat tgtctggcaa gtcttttaac tttgaaatag gagggtggcg tcgggctgct cataccgttg cagatcatat ttataccagg attactggca atggcgggta cttcgactca aaaattgcgt acattgcgga tatgctgggt gagagaagaa tagctctgat ggattatgat ggagctaaca gtgtttacgc ttctatagat aataatggat ggctatcttt ccctagattt tctcctgata caaaaagcat agtgtacact ctcacatcgc agtcttctcc ttctgggaga gttatgttgt acgatatgag gagagaggtt tcttcgctgc taataggcat gtctggtatt gtgtcgtcgg tgagattttc gccaagtggc cgcgaaatat tgctatcaga atcgcttaat ggcagcactg acatatactc ctttagtctt ataagcaaaa aacttacgag gctgaccgct gatgagttca tgaacacgtc tgcttcttat tcccctgatg agaaatctat agtatttgct tcggaccgag acggtggatc tcatctctat attatggatg ctaatggaag taacccacgc aaaataagct caggctcagg caggtacatg tctcctgtat ggtctcccaa cggaacttgg atagcattta ctaaggtcaa gggtgctcgt tgttatattg ggatcatgcg tcctaatggt agtaaggaaa gaatgatagc gtcggattgc tttgctgaag gaccgtcttg ggcgccgaat ggtcaggcag ttttatatac taaacatgtt caaagagtgg agcatttggc cacagaactt gctatggtag acttatcaag catgaatagg agggctataa atactccaag tgacgccttg atcgcacatt ggtcgaatac aatgcaaaat taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for AnphA.17299.a.A2.PS01013

PURIFICATION DATe:	3/24/2011
CONCENTRATION:	4.96mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	100µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCTCTTTG CGTCTAAGTA TCACTAAGGG CAACACTGGG AAACTAAGTA TAGCTTTTGC GCCCTTGCAC TCTTTTGAGA GCAATTTAGC GCAGAAGATT GGCGAGAGTT GCACAGATAT CATCTTAAAG GACTTGAACA ACACAGGGTT ATTTGATGCG CGTATTGAGA TTCTTGATCC AACCTCAGAT GGCGCAGCTT ACTCCAATGT ATGGAGAGAA TTGAAGCATG ACATCCTGCT TACAGGCAAT GTGCAGGAGC TTTCACACGG AAGAATGGGT ATCAAGTTAT TCGTGTGGGA TGTAGCTGCG GAAAAGAAAT TGTCTGGCAA GTCTTTTAAC TTTGAAATAG GAGGGTGGCG TCGGGCTGCT CATACCGTTG CAGATCATAT TTATACCAGG ATTACTGGCA ATGGCGGGTA CTTCGACTCA AAAATTGCGT ACATTGCGGA TATGCTGGGT GAGAGAAGAA TAGCTCTGAT GGATTATGAT GGAGCTAACA GTGTTTACGC TTCTATAGAT AATAATGGAT GGCTATCTTT CCCTAGATTT TCTCCTGATA CAAAAAGCAT AGTGTACACT CTCACATCGC AGTCTTCTCC TTCTGGGAGA GTTATGTTGT ACGATATGAG GAGAGAGGTT TCTTCGCTGC TAATAGGCAT GTCTGGTATT GTGTCGTCGG TGAGATTTTC GCCAAGTGGC CGCGAAATAT TGCTATCAGA ATCGCTTAAT GGCAGCACTG ACATATACTC CTTTAGTCTT ATAAGCAAAA AACTTACGAG GCTGACCGCT GATGAGTTCA TGAACACGTC TGCTTCTTAT TCCCCTGATG AGAAATCTAT AGTATTTGCT TCGGACCGAG ACGGTGGATC TCATCTCTAT ATTATGGATG CTAATGGAAG TAACCCACGC AAAATAAGCT CAGGCTCAGG CAGGTACATG TCTCCTGTAT GGTCTCCCAA CGGAACTTGG ATAGCATTTA CTAAGGTCAA GGGTGCTCGT TGTTATATTG GGATCATGCG TCCTAATGGT AGTAAGGAAA GAATGATAGC GTCGGATTGC TTTGCTGAAG GACCGTCTTG GGCGCCGAAT GGTCAGGCAG TTTTATATAC TAAACATGTT CAAAGAGTGG AGCATTTGGC CACAGAACTT GCTATGGTAG ACTTATCAAG CATGAATAGG AGGGCTATAA ATACTCCAAG TGACGCCTTG ATCGCACATT GGTCGAATAC AATGCAAAAT TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA