SSGCID: Target Page

AnphA.17081.x

Major surface protein 4

CENTER ID:	AnphA.17081.x
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.17081.x.B1.GE41148	full length		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1332
RefSeq:	YP_505768.1
UniProt:	Q2GIN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNYRELLVGS LSAAAVCACS LLISGSSFAY SGNNDASDVS GVMNGSFYVS GSYSPSFPSI SSFAISESDR GGSYVKGYNK NLSTLNVSDP ASFTQHDPSF KFAKSLLTSF DGATGYAIGG ARVEVEVGYK KFETLAESDY KHVESHNFVA VGRDATLTPD NFFVMKIDSV KDISVMLNAC YDVMHTDLPV SPYMCAGLGA SFINIADHVT SKLAYRGKVG VSYKLTPEIS LIAGGFYHGI FDEQYAGIPA SNRVNIAGGA AAKVKANIAS YGFNIGARFA FN

NT Sequence

atgaattaca gagaattgct tgtagggagc ctatctgcgg ccgcagtatg tgcctgctcc cttttaatta gtggatcctc atttgcgtat tcaggcaata atgatgcgtc tgatgttagc ggtgttatga acggcagctt ttacgtaagt ggtagctaca gtccgtcatt tccttctata tcttcatttg ctattagtga gtctgacagg ggaggatctt acgtaaaagg ttataacaag aatttgagca cgttgaatgt ttcagatcct gccagcttca cgcaacatga tccctcattc aagttcgcta agagtttact aacatctttt gacggtgcaa ctggctatgc tattggcgga gctagagtgg aagttgaagt tggatataaa aagtttgaaa cgctcgctga aagtgactac aagcatgtag aatcacataa ttttgttgct gtgggtcgtg acgcgacatt aactccagac aacttctttg taatgaaaat agacagcgtc aaagatatat ctgtaatgct taacgcttgt tacgacgtta tgcatactga cttgcctgta tctccttaca tgtgtgctgg gttaggggcg agctttatta atatcgccga tcacgttaca agtaagttgg cttatagagg aaaggtaggc gtaagttaca agcttactcc tgaaatatct ttaatagctg gaggttttta ccacggaatt ttcgatgaac agtatgcagg tattcccgct agtaaccgtg taaacatagc aggtggtgct gcagccaagg taaaggcaaa tatagctagt tacggcttca acataggagc aagatttgct ttcaattaa

Details for AnphA.17081.x.B1.GE41148

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN YRELLVGSLS AAAVCACSLL ISGSSFAYSG NNDASDVSGV MNGSFYVSGS YSPSFPSISS FAISESDRGG SYVKGYNKNL STLNVSDPAS FTQHDPSFKF AKSLLTSFDG ATGYAIGGAR VEVEVGYKKF ETLAESDYKH VESHNFVAVG RDATLTPDNF FVMKIDSVKD ISVMLNACYD VMHTDLPVSP YMCAGLGASF INIADHVTSK LAYRGKVGVS YKLTPEISLI AGGFYHGIFD EQYAGIPASN RVNIAGGAAA KVKANIASYG FNIGARFAFN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt acagagaatt gcttgtaggg agcctatctg cggccgcagt atgtgcctgc tcccttttaa ttagtggatc ctcatttgcg tattcaggca ataatgatgc gtctgatgtt agcggtgtta tgaacggcag cttttacgta agtggtagct acagtccgtc atttccttct atatcttcat ttgctattag tgagtctgac aggggaggat cttacgtaaa aggttataac aagaatttga gcacgttgaa tgtttcagat cctgccagct tcacgcaaca tgatccctca ttcaagttcg ctaagagttt actaacatct tttgacggtg caactggcta tgctattggc ggagctagag tggaagttga agttggatat aaaaagtttg aaacgctcgc tgaaagtgac tacaagcatg tagaatcaca taattttgtt gctgtgggtc gtgacgcgac attaactcca gacaacttct ttgtaatgaa aatagacagc gtcaaagata tatctgtaat gcttaacgct tgttacgacg ttatgcatac tgacttgcct gtatctcctt acatgtgtgc tgggttaggg gcgagcttta ttaatatcgc cgatcacgtt acaagtaagt tggcttatag aggaaaggta ggcgtaagtt acaagcttac tcctgaaata tctttaatag ctggaggttt ttaccacgga attttcgatg aacagtatgc aggtattccc gctagtaacc gtgtaaacat agcaggtggt gctgcagcca aggtaaaggc aaatatagct agttacggct tcaacatagg agcaagattt gctttcaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat