SSGCID: Target Page

AnphA.17081.t

P44-87 outer membrane protein, truncation

CENTER ID:	AnphA.17081.t
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.17081.t.A1.GE30761	Full length( AnphA.17081.t )		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.153
RefSeq:	YP_504767.1
UniProt:	Q2GLI4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEISHSEIDG KVCKTKSAGT GKNPCDHSQK PCSTNAYYAR RTQKSRSSGK TSLCGDSGYS GQELITGGHY SSPSVFRNFV KDTLQGNGSE NWPTSTGEGS ESNDNAIAVA KDLVNELTPE ERTIVAGLLA KIIEGSEVIE IRAISSTSVT MNICSDITIS NILMPYVCVG PGMSFVSVVD GHTAAKFAYR LKAGLSYKFS KEVTAFAGGF YHHVIGDGVY DDLPLRHLSD DISPVKHAKE TAIARFVMRY FGGEFGVRLA F

NT Sequence

GTGGAGATTT CTCATTCCGA GATTGATGGC AAGGTTTGTA AGACGAAGTC GGCGGGAACT GGAAAAAATC CGTGTGATCA TAGCCAAAAG CCGTGTAGTA CGAATGCGTA TTATGCGAGG AGAACGCAGA AGAGTAGGAG TTCGGGAAAA ACGTCTTTAT GCGGGGACAG TGGGTATAGC GGGCAGGAGC TAATAACGGG TGGGCATTAT AGCAGTCCAA GCGTATTCCG GAATTTTGTC AAAGACACAC TACAAGGAAA TGGTAGTGAG AACTGGCCTA CATCTACTGG AGAAGGAAGT GAGAGTAACG ACAACGCCAT AGCCGTTGCT AAGGACCTAG TAAATGAACT TACTCCTGAA GAACGAACCA TAGTGGCTGG GTTACTTGCT AAAATTATTG AAGGAAGCGA GGTTATTGAG ATTAGGGCCA TCTCTTCGAC TTCAGTTACA ATGAATATTT GCTCAGATAT CACGATAAGT AATATCTTAA TGCCGTATGT TTGTGTTGGT CCAGGGATGA GCTTTGTTAG TGTTGTTGAT GGTCACACTG CTGCAAAGTT TGCATATCGG TTAAAGGCAG GTCTGAGTTA TAAATTTTCG AAAGAAGTTA CAGCTTTTGC AGGTGGTTTT TACCATCACG TTATAGGAGA TGGTGTTTAT GATGATCTGC CATTGCGGCA TTTATCTGAT GATATTAGTC CTGTGAAACA TGCTAAGGAA ACCGCCATTG CTAGATTCGT CATGAGGTAC TTTGGCGGGG AATTTGGTGT TAGGCTCGCT TTT

Details for AnphA.17081.t.A1.GE30761

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVEISHSEI DGKVCKTKSA GTGKNPCDHS QKPCSTNAYY ARRTQKSRSS GKTSLCGDSG YSGQELITGG HYSSPSVFRN FVKDTLQGNG SENWPTSTGE GSESNDNAIA VAKDLVNELT PEERTIVAGL LAKIIEGSEV IEIRAISSTS VTMNICSDIT ISNILMPYVC VGPGMSFVSV VDGHTAAKFA YRLKAGLSYK FSKEVTAFAG GFYHHVIGDG VYDDLPLRHL SDDISPVKHA KETAIARFVM RYFGGEFGVR LAF

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg agatttctca ttccgagatt gatggcaagg tttgtaagac gaagtcggcg ggaactggaa aaaatccgtg tgatcatagc caaaagccgt gtagtacgaa tgcgtattat gcgaggagaa cgcagaagag taggagttcg ggaaaaacgt ctttatgcgg ggacagtggg tatagcgggc aggagctaat aacgggtggg cattatagca gtccaagcgt attccggaat tttgtcaaag acacactaca aggaaatggt agtgagaact ggcctacatc tactggagaa ggaagtgaga gtaacgacaa cgccatagcc gttgctaagg acctagtaaa tgaacttact cctgaagaac gaaccatagt ggctgggtta cttgctaaaa ttattgaagg aagcgaggtt attgagatta gggccatctc ttcgacttca gttacaatga atatttgctc agatatcacg ataagtaata tcttaatgcc gtatgtttgt gttggtccag ggatgagctt tgttagtgtt gttgatggtc acactgctgc aaagtttgca tatcggttaa aggcaggtct gagttataaa ttttcgaaag aagttacagc ttttgcaggt ggtttttacc atcacgttat aggagatggt gtttatgatg atctgccatt gcggcattta tctgatgata ttagtcctgt gaaacatgct aaggaaaccg ccattgctag attcgtcatg aggtactttg gcggggaatt tggtgttagg ctcgcttttt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatncnggc tgctaanaaa gcccgaaagg aagctgnnnt tggctgctgc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggagatt tctcattccg agattgatgg caaggtttgt aagacgaagt cggcgggaac tggaaaaaat ccgtgtgatc atagccaaaa gccgtgtagt acgaatgcgt attatgcgag gagaacgcag aagagtagga gttcgggaaa aacgtcttta tgcggggaca gtgggtatag cgggcaggag ctaataacgg gtgggcatta tagcagtcca agcgtattcc ggaattttgt caaagacaca ctacaaggaa atggtagtga gaactggcct acatctactg gagaaggaag tgagagtaac gacaacgcca tagccgttgc taaggaccta gtaaatgaac ttactcctga agaacgaacc atagtggctg ggttacttgc taaaattatt gaaggaagcg aggttattga gattagggcc atctcttcga cttcagttac aatgaatatt tgctcagata tcacgataag taatatctta atgccgtatg tttgtgttgg tccagggatg agctttgtta gtgttgttga tggtcacact gctgcaaagt ttgcatatcg gttaaaggca ggtctgagtt ataaattttc gaaagaagtt acagcttttg caggtggttt ttaccatcac gttataggag atggtgttta tgatgatctg ccattgcggc atttatctga tgatattagt cctgtgaaac atgctaagga aaccgccatt gctagattcg tcatgaggta ctttggcggg gaatttggtg ttaggctcgc tttttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa