AnphA.17081.s

P44-71 outer membrane protein

CENTER ID: AnphA.17081.s
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17081.s.A2.GE30760 Mature protein( AnphA.17081.s ) 26 429
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.714
RefSeq: YP_505245.1
UniProt: Q2GK56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVRSYSNLF LAGVMASIVG VTVSNAAKAD QDYGASLDAR RSSHFYIGLD YSPAFSKIRD FKIIESSGET AAVYPYFDDG RSHAIEYNKF DWNTPNPRIG FKDNTLLAIG SSIGYAFSRA RVEVEVGYES FKIKDRGKGG KKEKDGSAVY LLAKELAYSV ASNQQDKLAV ALSKVKGKDI VNFAKALEST SPTIGEKVCR TRSGSGEKNY ARYVEKTSRS SNDTSLCGDI GGTSAHNSPR VNVFKDFAAK TLGDGSRNWP TSSGTKSMEN DNANAVAADL VKNLNMEEKA IVAGLFSRTI EGGEAAKISA ISTTSVMLNV CYDFYGKESS IAPYACAGIG SNFVGIVDSH IVPKLAYKLK AGIGCQISPE ISIFAGGFYH RILGNGRYAD LPVKRLFYDE SPKGRTKNTA VANFSMSYAG GELGVRLAF
NT Sequence
ATGAGAGTTA GAAGTTATAG TAATCTGTTT TTAGCAGGAG TAATGGCATC CATAGTAGGA GTTACTGTTA GCAATGCCGC GAAAGCAGAT CAAGATTATG GAGCTTCTTT AGATGCTAGG AGGTCTAGTC ATTTTTACAT AGGGTTAGAT TATAGTCCGG CATTTAGTAA GATAAGAGAT TTTAAGATAA TAGAAAGTAG TGGTGAAACT GCGGCGGTAT ATCCGTATTT TGATGATGGG AGGAGCCATG CAATAGAATA CAATAAATTT GATTGGAATA CTCCAAATCC TCGTATAGGC TTTAAGGACA ATACACTTTT AGCTATAGGT AGCAGTATAG GTTATGCTTT TTCTAGAGCT AGAGTTGAAG TTGAAGTTGG CTACGAGTCC TTTAAGATAA AGGATCGGGG TAAGGGTGGA AAAAAAGAGA AGGATGGAAG TGCGGTATAT TTGCTCGCAA AGGAGCTTGC ATATAGTGTA GCATCTAATC AACAGGATAA GCTTGCTGTA GCATTATCTA AGGTTAAAGG GAAAGATATT GTTAATTTTG CAAAAGCCCT AGAAAGTACT AGCCCCACTA TTGGGGAAAA GGTTTGTCGA ACGCGCAGTG GAAGTGGTGA GAAAAATTAT GCTAGGTATG TAGAAAAAAC CTCGAGGTCC AGTAATGATA CTTCACTATG TGGAGATATT GGTGGAACCA GTGCTCATAA TAGTCCAAGG GTGAATGTCT TTAAGGATTT TGCGGCTAAA ACGCTAGGAG ATGGTAGTAG GAACTGGCCA ACATCTAGTG GGACTAAGAG TATGGAAAAC GATAATGCGA ACGCTGTAGC TGCAGATTTA GTTAAAAATC TCAATATGGA GGAGAAAGCC ATAGTAGCTG GATTATTTTC AAGGACTATT GAAGGGGGTG AGGCCGCAAA GATTAGTGCT ATTTCTACTA CTTCTGTTAT GCTGAATGTT TGTTATGATT TTTATGGAAA AGAGTCAAGT ATTGCTCCTT ACGCATGTGC AGGAATAGGT AGTAATTTCG TTGGTATAGT AGATAGCCAT ATCGTTCCTA AACTTGCTTA TAAATTAAAA GCTGGTATAG GCTGTCAGAT TTCTCCTGAA ATTTCAATTT TTGCAGGTGG GTTTTATCAT CGTATTTTAG GTAATGGGAG ATATGCGGAT CTTCCTGTAA AGCGCTTATT TTACGATGAA AGTCCAAAGG GGCGCACAAA AAATACCGCA GTTGCTAATT TCAGCATGAG TTATGCCGGT GGTGAGTTAG GTGTAAGGTT AGCCTTT
Details for AnphA.17081.s.A2.GE30760
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 46kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKADQDY GASLDARRSS HFYIGLDYSP AFSKIRDFKI IESSGETAAV YPYFDDGRSH AIEYNKFDWN TPNPRIGFKD NTLLAIGSSI GYAFSRARVE VEVGYESFKI KDRGKGGKKE KDGSAVYLLA KELAYSVASN QQDKLAVALS KVKGKDIVNF AKALESTSPT IGEKVCRTRS GSGEKNYARY VEKTSRSSND TSLCGDIGGT SAHNSPRVNV FXDFAAKTLG DGSRNWPTSS GTKSMENDNA NAVAADLVKN LNMEXKAIVA GLFSRTIEGG EXAKIXAIST XXVMLNVXXD FYGKXSSXAX XXMXXXVXXX XXXXXXXSXL XXLXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg cgaaagcaga tcaagattat ggagcttctt tagatgctag gaggtctagt catttttaca tagggttaga ttatagtccg gcatttagta agataagaga ttttaagata atagaaagta gtggtgaaac tgcggcggta tatccgtatt ttgatgatgg gaggagccat gcaatagaat acaataaatt tgattggaat actccaaatc ctcgtatagg ctttaaggac aatacacttt tagctatagg tagcagtata ggttatgctt tttctagagc tagagttgaa gttgaagttg gctacgagtc ctttaagata aaggatcggg gtaagggtgg aaaaaaagag aaggatggaa gtgcggtata tttgctcgca aaggagcttg catatagtgt agcatctaat caacaggata agcttgctgt agcattatct aaggttaaag ggaaagatat tgttaatttt gcaaaagccc tagaaagtac tagccccact attggggaaa aggtttgtcg aacgcgcagt ggaagtggtg agaaaaatta tgctaggtat gtagaaaaaa cctcgaggtc cagtaatgat acttcactat gtggagatat tggtggaacc agtgctcata atagtccaag ggtgaatgtc tttaangatt ttgcggctaa aacgctagga gatggtagta ggaactggcc aacatctagt gggactaaga gtatggaaaa cgataatgcg aacgctgtag ctgcagattt agttaaaaat ctcaatatgg agganaaagc catagtagct ggattatttt caaggactat tgaagggggt gaggncgcaa agattanngc tatttctact nnnnctgtna tgctgaatgt ttgnnatgat ttttatggna aanantcaag tnntgctncn tnncncatgc annannangt annnntttnn nnnnnnnann nnanagncnn atcnnnccta ancnngcttn nnannnaaan nnnngnannn ngnnnnncna nntnnnnnnn nttntnncng nnnnntnncn tcnnnnnnnn nnnnnnnnnn nggnnnnnnn nnnnnnnnnn nnnntntncn nnnnnnnnnn nnanngg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKADQDY GASLDARRSS HFYIGLDYSP AFSKIRDFKI IESSGETAAV YPYFDDGRSH AIEYNKFDWN TPNPRIGFKD NTLLAIGSSI GYAFSRARVE VEVGYESFKI KDRGKGGKKE KDGSAVYLLA KELAYSVASN QQDKLAVALS KVKGKDIVNF AKALESTSPT IGEKVCRTRS GSGEKNYARY VEKTSRSSND TSLCGDIGGT SAHNSPRVNV FKDFAAKTLG DGSRNWPTSS GTKSMENDNA NAVAADLVKN LNMEEKAIVA GLFSRTIEGG EAAKISAIST TSVMLNVCYD FYGKESSIAP YACAGIGSNF VGIVDSHIVP KLAYKLKAGI GCQISPEISI FAGGFYHRIL GNGRYADLPV KRLFYDESPK GRTKNTAVAN FSMSYAGGEL GVRLAF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgcgaaa gcagatcaag attatggagc ttctttagat gctaggaggt ctagtcattt ttacataggg ttagattata gtccggcatt tagtaagata agagatttta agataataga aagtagtggt gaaactgcgg cggtatatcc gtattttgat gatgggagga gccatgcaat agaatacaat aaatttgatt ggaatactcc aaatcctcgt ataggcttta aggacaatac acttttagct ataggtagca gtataggtta tgctttttct agagctagag ttgaagttga agttggctac gagtccttta agataaagga tcggggtaag ggtggaaaaa aagagaagga tggaagtgcg gtatatttgc tcgcaaagga gcttgcatat agtgtagcat ctaatcaaca ggataagctt gctgtagcat tatctaaggt taaagggaaa gatattgtta attttgcaaa agccctagaa agtactagcc ccactattgg ggaaaaggtt tgtcgaacgc gcagtggaag tggtgagaaa aattatgcta ggtatgtaga aaaaacctcg aggtccagta atgatacttc actatgtgga gatattggtg gaaccagtgc tcataatagt ccaagggtga atgtctttaa ggattttgcg gctaaaacgc taggagatgg tagtaggaac tggccaacat ctagtgggac taagagtatg gaaaacgata atgcgaacgc tgtagctgca gatttagtta aaaatctcaa tatggaggag aaagccatag tagctggatt attttcaagg actattgaag ggggtgaggc cgcaaagatt agtgctattt ctactacttc tgttatgctg aatgtttgtt atgattttta tggaaaagag tcaagtattg ctccttacgc atgtgcagga ataggtagta atttcgttgg tatagtagat agccatatcg ttcctaaact tgcttataaa ttaaaagctg gtataggctg tcagatttct cctgaaattt caatttttgc aggtgggttt tatcatcgta ttttaggtaa tgggagatat gcggatcttc ctgtaaagcg cttattttac gatgaaagtc caaaggggcg cacaaaaaat accgcagttg ctaatttcag catgagttat gccggtggtg agttaggtgt aaggttagcc ttttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa