SSGCID: Target Page

AnphA.17081.r

P44-70 outer membrane protein

CENTER ID:	AnphA.17081.r
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.17081.r.A2.GE30759	Mature protein( AnphA.17081.r )		47	442

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.713
RefSeq:	YP_505244.1
UniProt:	Q2GK57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVKGNGSIFM TSVLGVRCMS ARSCRKLFLG SIMLSFAVLM TSQEVQAMHS GQNISDMGES NYFYVGLNYS PVFSKIRDFT ISESSGETKA VYPYLRDGKS VKLETNRFDW SVPDPQIRFK NSVLLAMEGS IGYTMGALRF EVEVGYERFK IKSRYGKGIR GEESDTIYLL AKELAYDVVA NKTDALAAAL AKVKGQDIVH FANTLKITHP WINEQICNRG NDLLDNGGLK DSKKGSCNSK DKKGGKLEQS FTAALGDQGA QKWPKINNGG KKLTGKSNGK PYALDASATV AGDISVLDRE SKVMVAGLLA RTIEGSEIVE IKAISSTSVM LNACYDIFSE GFGIMPYTCI GLGSNFVSIV DGKVTPKLSY KLKAGLSYKF SPEVSAFAGG FYQRVVGDGE YVDLPVKRLS YDLSPQGRTK ETAVAKFSMS YVGGEFGIRF AF

NT Sequence

ATGGTTAAGG GGAATGGGTC GATTTTCATG ACCTCAGTTT TAGGAGTTAG GTGTATGAGC GCTAGAAGTT GTAGGAAGCT GTTTTTAGGA AGTATAATGC TGTCTTTTGC GGTGCTAATG ACTAGTCAGG AAGTCCAAGC TATGCATTCT GGACAGAATA TTTCCGATAT GGGAGAATCT AATTATTTCT ATGTAGGATT AAATTATAGT CCAGTATTTA GCAAGATAAG AGATTTTACT ATAAGTGAAA GCAGTGGAGA AACCAAGGCA GTATATCCAT ACCTAAGGGA TGGGAAGAGC GTAAAATTAG AGACCAATAG ATTTGATTGG AGTGTACCTG ATCCGCAAAT TAGGTTTAAG AACAGCGTGC TACTAGCTAT GGAAGGAAGT ATTGGCTATA CCATGGGTGC TCTCAGATTT GAAGTTGAAG TTGGTTATGA GCGTTTTAAA ATCAAAAGTC GCTACGGCAA GGGTATAAGA GGAGAGGAAT CCGATACAAT ATATCTACTA GCTAAGGAGC TGGCTTATGA TGTTGTTGCA AACAAAACTG ACGCGCTTGC TGCCGCTCTT GCGAAAGTGA AAGGACAAGA CATTGTTCAT TTTGCCAATA CTCTGAAGAT TACTCATCCT TGGATTAATG AGCAAATTTG CAATAGAGGA AACGATCTTT TGGACAACGG TGGGCTCAAA GACAGTAAGA AAGGTTCTTG CAATAGTAAA GATAAAAAGG GCGGAAAGTT GGAGCAGAGC TTTACAGCGG CTTTAGGTGA TCAAGGAGCG CAGAAGTGGC CTAAGATCAA TAATGGAGGT AAAAAATTAA CCGGTAAAAG CAACGGGAAG CCTTATGCGT TGGATGCATC AGCTACTGTA GCAGGGGATA TCAGTGTTCT TGATAGGGAA TCAAAAGTAA TGGTGGCAGG TCTATTAGCC AGGACTATAG AAGGAAGTGA GATTGTAGAG ATAAAAGCAA TTTCTTCCAC GTCTGTCATG CTTAACGCTT GTTATGATAT TTTCAGTGAG GGGTTTGGTA TTATGCCGTA TACCTGCATT GGTCTTGGGA GTAACTTTGT TAGTATAGTT GATGGAAAAG TAACTCCGAA GTTATCTTAC AAGTTAAAGG CAGGATTGAG TTATAAGTTC TCTCCTGAAG TTTCAGCATT TGCAGGAGGG TTTTATCAGC GTGTTGTTGG TGATGGAGAA TATGTAGATC TTCCGGTAAA GCGCTTATCT TATGATCTTA GTCCTCAGGG ACGTACAAAA GAAACGGCTG TTGCGAAATT CAGCATGAGT TATGTAGGTG GTGAATTTGG CATAAGGTTT GCTTTT

Details for AnphA.17081.r.A2.GE30759

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	45kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	66

Validated AA Sequence

Validated NT Sequence

attttnttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta tgcattctgg acagaatatt tccgatatgg gagaatctaa ttatttctat gtaggattaa attatagtcc agtatttagc aagataagag attttactat aagtgaaagc agtggagaaa ccaaggcagt atatccatac ctaagggatg ggaagagcgt aaaattagag accaatagat ttgattggag tgtacctgat ccgcaaatta ggtttaagaa cagcgtgcta ctagctatgg aaggaagtat tggctatacc atgggtgctc tcagatttga agttgaagtt ggttatgagc gttttaaaat caaaagtcgc tacggcaagg gtataagagg agaggaatcc gatacaatat atctactagc taaggagctg gcttatgatg ttgttgcaaa caaaactgac gcgcttgctg ccgctcttgc gaaagtgaaa ggacaagaca ttgttcattt tgccaatact ctgaagatta ctcatccttg gattaatgag caaatttgca atanaggaaa cgatcttttg gacaacggtg ggctcaaaga cagtaagaaa ggttcttgcn ntagtaaaga taaaaagggc ggaaagttgg agcagagctt tacagcggct ttaggtgatc aaggagcgca gaantggcct aagatcaata atggaggnaa aaaattaacc ggtaaaagca acgggaagcc ttatgcgttg gatgcatcag ctactgtagc nnggatatca gtgttcttgn tagggaatca aaagtaatgg tggcnggtct attagccang actatagnnn naantgagat tgtagagana aaagcaattt cttccncgtc tgtcatgctt aacgcttgtt atganntttt cagtgagggg nggnnntatg ncgtanacct ncntnnnncn tgggnngtnn cnnngntant nntantnnat ggnaaagtnn ntccnnnnnn nctnnannnn naggcaggan gagttannnt ncnnnncnnn nnnnnncnnn nnttnnnnan annnnttnnc nnnnnnnntn nnnnnnnngn nnannnngnn nnannnnnnn nnnnnnnnnn cnncnnnnnn nnnnnnaann nannnnnnnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAMHSGQNI SDMGESNYFY VGLNYSPVFS KIRDFTISES SGETKAVYPY LRDGKSVKLE TNRFDWSVPD PQIRFKNSVL LAMEGSIGYT MGALRFEVEV GYERFKIKSR YGKGIRGEES DTIYLLAKEL AYDVVANKTD ALAAALAKVK GQDIVHFANT LKITHPWINE QICNRGNDLL DNGGLKDSKK GSCNSKDKKG GKLEQSFTAA LGDQGAQKWP KINNGGKKLT GKSNGKPYAL DASATVAGDI SVLDRESKVM VAGLLARTIE GSEIVEIKAI SSTSVMLNAC YDIFSEGFGI MPYTCIGLGS NFVSIVDGKV TPKLSYKLKA GLSYKFSPEV SAFAGGFYQR VVGDGEYVDL PVKRLSYDLS PQGRTKETAV AKFSMSYVGG EFGIRFAF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctatgcat tctggacaga atatttccga tatgggagaa tctaattatt tctatgtagg attaaattat agtccagtat ttagcaagat aagagatttt actataagtg aaagcagtgg agaaaccaag gcagtatatc catacctaag ggatgggaag agcgtaaaat tagagaccaa tagatttgat tggagtgtac ctgatccgca aattaggttt aagaacagcg tgctactagc tatggaagga agtattggct ataccatggg tgctctcaga tttgaagttg aagttggtta tgagcgtttt aaaatcaaaa gtcgctacgg caagggtata agaggagagg aatccgatac aatatatcta ctagctaagg agctggctta tgatgttgtt gcaaacaaaa ctgacgcgct tgctgccgct cttgcgaaag tgaaaggaca agacattgtt cattttgcca atactctgaa gattactcat ccttggatta atgagcaaat ttgcaataga ggaaacgatc ttttggacaa cggtgggctc aaagacagta agaaaggttc ttgcaatagt aaagataaaa agggcggaaa gttggagcag agctttacag cggctttagg tgatcaagga gcgcagaagt ggcctaagat caataatgga ggtaaaaaat taaccggtaa aagcaacggg aagccttatg cgttggatgc atcagctact gtagcagggg atatcagtgt tcttgatagg gaatcaaaag taatggtggc aggtctatta gccaggacta tagaaggaag tgagattgta gagataaaag caatttcttc cacgtctgtc atgcttaacg cttgttatga tattttcagt gaggggtttg gtattatgcc gtatacctgc attggtcttg ggagtaactt tgttagtata gttgatggaa aagtaactcc gaagttatct tacaagttaa aggcaggatt gagttataag ttctctcctg aagtttcagc atttgcagga gggttttatc agcgtgttgt tggtgatgga gaatatgtag atcttccggt aaagcgctta tcttatgatc ttagtcctca gggacgtaca aaagaaacgg ctgttgcgaa attcagcatg agttatgtag gtggtgaatt tggcataagg tttgcttttt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa